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| 1 | Dispersive liquid-liquid microextraction,an effective tool for the determination of synthetic cannabinoids in oral fluid by liquid chromatography-tandem mass spectrometry显示文摘In the present work,dispersive liquid-liquid microextraction(DLLME)was used to extract six synthetic cannabinoids(JWH-018,JWH-019,JWH-073,JWH-200,or WIN 55,225,JWH-250,and AM-694)from oral fluids.A rapid baseline separation of the analytes was achieved on a bidentate octadecyl silica hydride phase(Cogent Bidentate C18;4.6 mm×250 mm,4μm)maintained at 37℃,by eluting in isocratic conditions(water:acetonitrile(25:75,V/V)).Detection was performed using positive electrospray ionization-tandem mass spectrometry.The parameters affecting DLLME(pH and ionic strength of the aqueous phase,type and volume of the extractant and dispersive solvent,vortex and centrifugation time)were optimized for maximizing yields.In particular,using 0.5 mL of oral fluid,acetonitrile(1 mL),was identified as the best option,both as a solvent to precipitate proteins and as a dispersing solvent in the DLLME procedure.To select an extraction solvent,a low transition temperature mixture(LTTM;composed of sesamol and chlorine chloride with a molar ratio of 1:3)and dichloromethane were compared;the latter(100μL)was proved to be a better extractant,with recoveries ranging from 73%to 101%by vortexing for 2 min.The method was validated according to the guidelines of Food and Drug Administration bioanalytical methods:intra-day and inter-day precisions ranged between 4%and 18%depending on the spike level and analyte;limits of detection spanned from 2 to 18 ng/mL;matrixmatched calibration curves were characterized by determination coefficients greater than 0.9914.Finally,the extraction procedure was compared with previous methods and with innovative techniques,presenting superior reliability,rapidity,simplicity,inexpensiveness,and efficiency. | Pierpaolo Tomai Alessandra Gentili Roberta Curini Rossella Gottardo Franco Tagliaro Salvatore Fanali | 2021 | Journal of Pharmaceutical Analysis2021，11，3： | 4 |
| 2 | Tumor microenvironment involvement in colorectal cancer progression via Wnt/β-catenin pathway:Providing understanding of the complex mechanisms of chemoresistance显示文摘Colorectal cancer(CRC)continues to be one of the main causes of death from cancer because patients progress unfavorably due to resistance to current therapies.Dysregulation of the Wnt/β-catenin pathway plays a fundamental role in the genesis and progression of several types of cancer,including CRC.In many subtypes of CRC,hyperactivation of theβ-catenin pathway is associated with mutations of the adenomatous polyposis coli gene.However,it can also be associated with other causes.In recent years,studies of the tumor microenvironment(TME)have demonstrated its importance in the development and progression of CRC.In this tumor nest,several cell types,structures,and biomolecules interact with neoplastic cells to pave the way for the spread of the disease.Cross-communications between tumor cells and the TME are then established primarily through paracrine factors,which trigger the activation of numerous signaling pathways.Crucial advances in the field of oncology have been made in the last decade.This Minireview aims to actualize what is known about the central role of the Wnt/β-catenin pathway in CRC chemoresistance and aggressiveness,focusing on crosscommunication between CRC cells and the TME.Through this analysis,our main objective was to increase the understanding of this complex disease considering a more global context.Since many treatments for advanced CRC fail due to mechanisms involving chemoresistance,the data here exposed and analyzed are of great interest for the development of novel and effective therapies. | María Belén Novoa Díaz María Julia Martín Claudia Gentili | 2022 | World Journal of Gastroenterology2022，28，26： | 3 |
| 3 | Impact of HLA-G analysis in prevention, diagnosis and treatment of pathological conditions显示文摘Human leukocyte antigen-G(HLA-G) is a non-classical HLA class Ⅰ molecule that differs from classical HLA class Ⅰ molecules by low polymorphism and tissue distribution. HLA-G is a tolerogenic molecule with an immune-modulatory and anti-inflammatory function on both innate and adaptative immunity. This peculiar characteristic of HLA-G has led to investigations of its role in pathological conditions in order to define possible uses in diagnosis, prevention and treatment. In recent years, HLA-G has been shown to have an important implication in different inflammatory and autoimmune diseases, pregnancy complications, tumor development and aggressiveness, and susceptibility to viral infections. In fact, HLA-G molecules have been reported to alternate at both genetic and protein level in different disease situations, supporting its crucial role in pathological conditions. Specific pathologies show altered levels of soluble(s)HLA-G and different HLA-G gene polymorphisms seem to correlate with disease. This review aims to update scientific knowledge on the contribution of HLA-G in managing pathological conditions. | Daria Bortolotti Valentina Gentili Antonella Rotola Enzo Cassai Roberta Rizzo Dario Di Luca | 2014 | World Journal of Methodology2014，4，1： | 3 |
| 4 | Incidental extravascular findings in computed tomographic angiography for planning or monitoring endovascular aortic aneurysm repair: Smoker patients, increased lung cancer prevalence?显示文摘AIM To validate the feasibility of high resolution computed tomography(HRCT) of the lung prior to computed tomography angiography(CTA) in assessing incidental thoracic findings during endovascular aortic aneurysm repair(EVAR) planning or follow-up.METHODS We conducted a retrospective study among 181 patients(143 men, mean age 71 years, range 50-94) referred to our centre for CTA EVAR planning or followup. HRCT and CTA were performed before or after 1 or 12 mo respectively to EVAR in all patients. All HRCT examinations were reviewed by two radiologists with 15 and 8 years experience in thoracic imaging. The results were compared with histology, bronchoscopy or follow-up HRCT in 12, 8 and 82 nodules respectively. RESULTS There were a total of 102 suspected nodules in 92 HRCT examinations, with a mean of 1.79 nodules per patient and an average diameter of 9.2 mm(range 4-56 mm). Eightynine out of 181 HRCTs resulted negative for the presence of suspected nodules with a mean smoking history of 10 pack-years(p-y, range 5-18 p-y). Eighty-two out of 102(76.4%) of the nodules met criteria for computed tomography follow-up, to exclude the malignant evolution. Of the remaining 20 nodules, 10 out of 20(50%) nodules, suspected for malignancy, underwent biopsy and then surgical intervention that confirmed the neoplastic nature: 4(20%) adenocarcinomas, 4(20%) squamous cell carcinomas, 1(5%) small cell lung cancer and 1(5%) breast cancer metastasis); 8 out of 20(40%) underwent bronchoscopy(8 pneumonia) and 2 out of 20(10%) underwent biopsy with the diagnosis of sarcoidosis.CONCLUSION HRCT in EVAR planning and follow-up allows to correctly identify patients requiring additional treatments, especially in case of lung cancer. | Maria Antonietta Mazzei Susanna Guerrini Francesco Gentili Giuseppe Galzerano Francesco Setacci Domenico Benevento Francesco Giuseppe Mazzei Luca Volterrani Carlo Setacci | 2017 | World Journal of Radiology2017，9，7： | 3 |
| 5 | Cognitive impairment in multiple sclerosis: lessons from cerebrospinal fluid biomarkers显示文摘Cognitive impairment is a common clinical manifestation of multiple sclerosis,but its pathophysiology is not completely understood.White and grey matter injury together with synaptic dysfunction do play a role.The measurement of biomarkers in the cerebrospinal fluid and the study of their association with cognitive impairment may provide interesting in vivo evidence of the biological mechanisms underlying multiple sclerosis-related cognitive impairment.So far,only a few studies on this topic have been published,giving interesting results that deserve further investigation.Cerebrospinal fluid biomarkers of different pathophysiological mechanisms seem to reflect different neuropsychological patterns of cognitive deficits in multiple sclerosis.The aim of this review is to discuss the studies that have correlated cerebrospinal fluid markers of immune,glial and neuronal pathology with cognitive impairment in multiple sclerosis.Although preliminary,these findings suggest that cerebrospinal fluid biomarkers show some correlation with cognitive performance in multiple sclerosis,thus providing interesting insights into the mechanisms underlying the involvement of specific cognitive domains. | Lorenzo Gaetani Nicola Salvadori Elena Chipi Lucia Gentili Angela Borrelli Lucilla Parnetti Massimiliano Di Filippo | 2021 | Neural Regeneration Research2021，16，1： | 2 |
| 6 | Estimates of sea surface nitrate concentrations from sea surface temperature and chlorophyll eoneetltration in upwelling areas a case study for the Benguela system 显示文摘 | Aria Sili6-Calzada Atmick Bdcaud Bernard Gentili | 2008 | Remote Sensing of Environment2008，112，： | 1 |
| 7 | Expression of the extracellular fatty acid binding protein (Ex-FABP)during muscle fiber formation显示文摘 | Gentili C Cemetfi S Tacchei C | 1998 | Exp Cell Res1998，242，2： | 1 |
| 8 | The CT, MRI, and radiographic appearance of lipohemarthrosis显示文摘 | Sorenson S Gentili A Seeger LL | 2001 | AJR2001，176，2： | 1 |
| 9 | Minimum weighted feedback verlex set on diamonds 显示文摘 | Carrabs F Cerulli R Gentili M | 2005 | Information Processing Letters2005，94，1： | 1 |
| 10 | The association between polycystic kidney disease and cerebral aneurysms显示文摘 | Fehlings MG Gentili F | 1991 | Can J Neurol Sci1991，18，4： | 1 |
| 11 | High- and lowresolution mass spectrometry coupled to liquid chromatography as confirmatory methods of anabolic residues in crude meat and infant foods 显示文摘 | GENTILI A SERGI M PERRET D | 2006 | Rapid Communications in Mass Spectrometry2006，20，12： | 1 |
| 12 | Comparing the catalytic efficiency of some mediators of laccase显示文摘 | Fabbrini M C Galli P Gentili | 2002 | Journal of Molecular Catalysis B:Enzymatic2002，16，56： | 1 |
| 13 | Anterior cruciate ligament tear: indirect signs at MRimaging显示文摘 | Gentili A Seeger LL Yao L | 1994 | Radiology1994，193，3： | 1 |
| 14 | Lymphadenectomy in the differentiated thyroid carcinoma显示文摘 | Montesani C Gentili V Pronio A | | 0，，： | 1 |
| 15 | A foreign body aspiration in a preschool child mimicking a multitrigger wheezing: a case report and review of the literature显示文摘 | Bertelli L Gentili A Modolon C | 2012 | Pediatr Emerg Care2012，28，12： | 1 |
| 16 | Infraclavicular with lateral approach and nerve stimulation: Extent of anesthesia and adverse effects显示文摘 | Jandard C Gentili ME< Girard F Ecoffey C Heck M Laxenaire MC Bouaziz H | 2002 | Reg Anesth Pain Med2002，27，1： | 1 |
| 17 | Diffuse Reflectance of Oceanic Shallow Waters:Influence of Water Depth and Bottom Albedo显示文摘 | Morel A Gentili B | 1994 | Limnology & Oceanography1994，39，： | 1 |
| 18 | Liquid chromatngraphy-tandem mass spectrometry for peffo Tning confirmatory analysis of veterinary drugs in animal-food products显示文摘 | Gentili A Perret D Marchese S | 2005 | Trends Anal Chem2005，24，： | 1 |
| 19 | Infraclavicular with lateral approach and nerve stimulation:Extent of anesthesia and adverse effects显示文摘 | Jandard C Gentili ME Girard F | 2002 | Reg Anesth Pain Med2002，27，： | 1 |
| 20 | A comparison of a single-stimulation lateral infraclavicular plexus block with a triple stimulation block显示文摘 | Delenze A Gentili ME Marret E | 2003 | Reg Anesth Pain Med2003，28，6： | 1 |
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