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| 1 | Ephrin-B2 is differentially expressed in the intestinal epithelium in Crohn's disease and contributes to accelerated epithelial wound healing in vitro显示文摘AIM: Eph receptor tyrosine kinases and their membrane bound receptor-like ligands, the ephrins, represent a bi-directional cell-cell contact signaling system that directs epithelial movements in development. The meaning of this system in the adult human gut is unknown. We investigated the Eph/ephrin mRNA expression in the intestinal epithelium of healthy controls and patients with inflammatory bowel disease (IBD).METHODS: mRNA expression profiles of all Eph/ephrin family members in normal small intestine and colon were established by real-time RT-PCR. In addition, differential expression in IBD was investigated by cDNA array technology, and validated by both real-time RT-PCR and immunohistochemistry. Potential effects of enhanced EphB/ephrin-B signaling were analyzed in an in vitro IEC-6 cell scratch wound model.RESULTS: Human adult intestinal mucosa exhibits a complex pattern of Eph receptors and ephrins. Beside the known prominent co-expression of EphA2 and ephrinA1,we found abundantly co-expressed EphB2 and ephrin-B1/2.Interestingly, cDNA array data, validated by real-time PCR and immunohistochemistry, showed upregulation of ephrin-B2 in both perilesional and lesional intestinal epithelial cells of IBD patients, suggesting a role in epithelial homeostasis. Stimulation of ephrin-B signaling in ephrinB1/2 expressing rat IEC-6-cells with recombinant EphB1Fc resulted in a significant dose-dependent acceleration of wound closure. Furthermore, fluorescence microscopy showed that EphB1-Fc induced coordinated migration of wound edge cells is associated with enhanced formation of lamellipodial protrusions into the wound, increased actin stress fiber assembly and production of laminin at the wound edge.CONCLUSION: EphB/ephrin-B signaling might represent a novel protective mechanism that promotes intestinal epithelial wound healing, with potential impact on epithelial restitution in IBD. | Christian Hafner Stefanie Meyer Thomas Langmann Gerd Schmitz Frauke Bataille Ilja Hagen Bernd Becker Alexander Roesch Gerhard Rogler Michael Landthaler Thomas Vogt | 2005 | World Journal of Gastroenterology2005，11，26： | 9 |
| 2 | Quantification of Obstructive and Nonobstructive Coronary Lesions by 64-Slice Computed Tomography显示文摘 | Alexander W. Leber Andreas Knez Franz von Ziegler Alexander Becker Konstantin Nikolaou Stephan Paul Bernd Wintersperger Maximilian Reiser Christoph R. Becker Gerhard Steinbeck Peter Boekstegers | 2005 | Journal of the American College of Cardiology2005，，1： | 4 |
| 3 | Quantification of Obstructive and Nonobstructive Coronary Lesions by 64-Slice Computed Tomography显示文摘 | Alexander W. Leber Andreas Knez Franz von Ziegler Alexander Becker Konstantin Nikolaou Stephan Paul Bernd Wintersperger Maximilian Reiser Christoph R. Becker Gerhard Steinbeck Peter Boekstegers | 2005 | Journal of the American College of Cardiology2005，，1： | 2 |
| 4 | Usefulness of multislice spiral computed tomography angiography for determination of coronary artery stenoses显示文摘 | Andreas Knez Christoph R Becker Alexander Leber Bernd Ohnesorge Alexander Becker Carl White Ralph Haberl Maximilian F Reiser Gerhard Steinbeck | 2001 | The American Journal of Cardiology2001，，10： | 1 |
| 5 | Usefulness of multislice spiral computed tomography angiography for determination of coronary artery stenoses显示文摘 | Andreas Knez Christoph R Becker Alexander Leber Bernd Ohnesorge Alexander Becker Carl White Ralph Haberl Maximilian F Reiser Gerhard Steinbeck | 2001 | The American Journal of Cardiology2001，，10： | 1 |
| 6 | Adjuvant versus Neoadjuvant Radiochemotherapy for Locally Advanced Rectal Cancer A Progress Report of a Phase-III Randomized Trial (Protocol CAO/ARO/AIO-94)显示文摘 | Rolf Sauer Rainer Fietkau Christian Wittekind Peter Martus Claus R?del Werner Hohenberger Gerhard Jatzko Hubert Sabitzer Johann-Hinrich Karstens Heinz Becker Clemens Hess Rudolf Raab | 2001 | Strahlentherapie und Onkologie2001，，4： | 1 |
| 7 | Determination of Polycyclic Aromatic Sulfur Heterocyclic Compounds in Airborne Particulate by Gas Chromatography With Atomic Emission and Mass Spectrometric Detection 显示文摘 | Gerhard Becker Ulrika Nilsson Anders Colmsjo | | Journal of Chromatography A0，，： | 1 |
| 8 | Accuracy of 64-Slice Computed Tomography to Classify and Quantify Plaque Volumes in the Proximal Coronary System显示文摘 | Alexander W. Leber Alexander Becker Andreas Knez Franz von Ziegler Marc Sirol Konstantin Nikolaou Bernd Ohnesorge Zahi A. Fayad Christoph R. Becker Maximilian Reiser Gerhard Steinbeck Peter Boekstegers | 2006 | Journal of the American College of Cardiology2006，，： | 1 |
| 9 | Usefulness of multislice spiral computed tomography angiography for determination of coronary artery stenoses显示文摘 | Andreas Knez Christoph R Becker Alexander Leber Bernd Ohnesorge Alexander Becker Carl White Ralph Haberl Maximilian F Reiser Gerhard Steinbeck | 2001 | The American Journal of Cardiology2001，，10： | 1 |
| 10 | Rasmussen encephalitis: Incidence and course under randomized therapy with tacrolimus or intravenous immunoglobulins显示文摘 | Christian G. Bien Henning Tiemeier Robert Sassen Stefan Kuczaty Horst Urbach Marec von Lehe Albert J. Becker Thomas Bast Peter Herkenrath Michael Karenfort Bernd Kruse Gerhard Kurlemann Sabine Rona Susanne Schubert‐Bast Silvia Vieker Stefan Vlaho Bernd Wi | 2012 | Epilepsia2012，，3： | 1 |
| 11 | Rasmussen encephalitis: Incidence and course under randomized therapy with tacrolimus or intravenous immunoglobulins显示文摘 | Christian G. Bien Henning Tiemeier Robert Sassen Stefan Kuczaty Horst Urbach Marec von Lehe Albert J. Becker Thomas Bast Peter Herkenrath Michael Karenfort Bernd Kruse Gerhard Kurlemann Sabine Rona Susanne Schubert‐Bast Silvia Vieker Stefan Vlaho Bernd Wi | 2012 | Epilepsia2012，，3： | 1 |
| 12 | Global backtracking of anthropogenic radionuclides by means of a receptor oriented ensemble dispersion modelling system in support of Nuclear-Test-Ban Treaty verification 显示文摘 | Andreas Becker Gerhard Wotawa | 2007 | Atmospheric Environment2007，41，： | 1 |
| 13 | Thyroid and ovarian function in infertile women显示文摘 | Gerhard I Becker T Eggert-Kruse W | 1991 | Hum Reprod1991，6，3： | 1 |
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