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| 1 | Interferon-α response in chronic hepatitis B-transfected HepG2.2.15 cells is partially restored by lamivudine treatment显示文摘AIM: To characterize the IFN-response and its modul- ation by the antiviral compound lamivudine in HBV- transfected HepG2.2.15 cells. METHODS: HepG2.2.15 and HepG2 cells were stimulated with various concentrations of IFN-a2a in the presence or absence of lamivudine. Then, total RNA was extracted and analysed by customised cDNA arrays and northern blot for interferon-inducible genes (ISGs). In addition, cellular proteins were extracted for EMSA and western blot. HBV replication was assessed by southern blot or ELISAs for HBsAg and HBeAg. RESULTS: Two genes (MxA, Cig5) with completely abolished and 4 genes (IFITM1, -2, -3, and 6-16) with partially reduced IFN-responses were identified in HepG2.2.15 cells. In 2 genes (IFITM1, 6-16), the response to IFN-a could be restored by treatment with lamivudine. This effect could not be explained by a direct modulation of the Jak/Stat signalling pathway since EMSA and western blot experiments revealed no suppression of Stat1 activation and ISGF3 formation after stimulation with IFN-a in HepG2.2.15 compared to HepG2 cells. CONCLUSION: These results are consistent with the assumption that chronic hepatitis B may specifically modulate the cellular response to IFN by a selective blockage of some ISGs. Antiviral treatment with lamivudine may partially restore ISG expression by reducing HBV gene expression and replication. | Shi-He Guan Mengji Lu Petra Grünewald Michael Roggendorf Guido Gerken Joerg F Schlaak | 2007 | World Journal of Gastroenterology2007，13，2： | 22 |
| 2 | Turnover rate and kinetic mechanism for the reaction of hydrodechlorination of 1, 1-dichlorotertrafluoroethane(CF3-CFCl2) over a polycrystalling Pd foil显示文摘 | Riberio F H Gerken C A Somorjai G A | 1997 | Catal Lett1997，45，： | 1 |
| 3 | Effects of tiotropium on lung hyperinflation dyspnoea and exercise tolerance in COPD显示文摘 | O'Donnell DE Fluge T Gerken F | 2004 | Eur Respir J2004，23，6： | 1 |
| 4 | Effects of tiotropioum on lung hyperinflation, dyspnoea and exercise tolerance in COPD显示文摘 | O'Donnell DE Fluge T Gerken F | 2004 | Eur Respir J2004，23，6： | 1 |
| 5 | Effects of tiotropium on lung hy-perinflation ,dyspnea,and exercise tolerance in patients with COPD显示文摘 | Donnell DE Fluge T Gerken F | 2004 | Eur Respir J2004，23，6： | 1 |
| 6 | Effects of tiotropium onlung hyperinflation,dyspnoea and exercise tolerance in COPD显示文摘 | O’Donnell D E Flüge T Gerken F | 2004 | EurRespir J2004，23，6： | 1 |
| 7 | Effects of tiotropium on lung hyperinflation, dyspnea and exercise tolerance in COPD 显示文摘 | O' Donnell DE Fluge T Gerken F | 2004 | Eur Respir J2004，23，： | 1 |
| 8 | Effects of tiotropium onlung hyperinflation dyspnoea and exercise tolerance in COPD显示文摘 | O'Donnell DE Flfige T Gerken F | 2004 | Eur Respir J2004，23，6： | 1 |
| 9 | Effects of tiotmpium onlung hy- perinflation, dyspnea and exercise tolerance in patients with COPD 显示文摘 | O'donnell DE Flidge T Gerken F | 2004 | Eur Respir J2004，23，5： | 1 |
| 10 | Effects of tiotropium on lung hyperinflation,dyspnoea and exercise tolerance in COPD显示文摘 | O'DONNELL DE FLUGE T GERKEN F | 2004 | Eur Respir J2004，23，6： | 1 |
| 11 | Effects of tiotropium on lung hyperinflation, dyspnoea and exercise tolerance in COPD显示文摘 | O' Donnell DE Fltige T Gerken F | 2004 | Eur Respir J2004，23，6： | 1 |
| 12 | Effects of tiotropium on lung hyperfintfation,dyspnoea and exercise tolerance in COPD显示文摘 | O'Donnell D E Fluge T Gerken F | 2004 | Eur Respir J2004，23，6： | 1 |
| 13 | Effects of tiotropium on lung hyperintlation, dyspnoea and exercise tolerance in COPD 显示文摘 | O'Donnell DE FlUge T Gerken F | 2004 | Eur Respir J2004，23，6： | 1 |
| 14 | Proteomics in Myzus persicae:effect of aphid host plant switch显示文摘 | Francis F Gerkens P Harmel N | 2006 | Insect Biochem Mol Biol2006，36，3： | 1 |
| 15 | Simultaneous determi- nation of 2,4-D and MCPA in canine plasma and urine by HPLC with fluorescence detection using 9-anthryldiazomethane (ADAM) 显示文摘 | Dickow L M Gerken D F Sams R A | 2001 | Joumal of Analytical Toxicology2001，25，1： | 1 |
| 16 | Infant sensitivity to distributional information can affect phonetic discrimination显示文摘 | MAYE J WERKER J F GERKEN L | 2002 | Cognition2002，82，： | 1 |
| 17 | Effects of tiotropiam on lung hyperinflation, dyspnea, and exercise tolerance in patients with COPD显示文摘 | Donnell DE Fluge T Gerken F | 2004 | Eur Respir J2004，23，： | 1 |
| 18 | Effects of tiotropium on lung hyperinflation, dyspnoea and exercise tolerance in COPD 显示文摘 | O'Donnell DE Fluge T Gerken F | 2004 | Eur Respir J2004，23，7： | 1 |
| 19 | Effects of tiotropium on lung hyperinflation,dyspnoea and exercise tolerance in COPD显示文摘 | O'Donnell DE Fluge T Gerken F | 2004 | Eur Respir J2004，23，7： | 1 |
| 20 | Turnover rate and kinetic mechanism for the reaction of hydrodechlorination of 1,1-dichlorotetrafluoroethane ( CF3 -CFC12 ) over a polycrystalline Pd foil 显示文摘 | Ribeiro F H Gerken C A Somorjai G A | 1997 | Catal Lett1997，45，34： | 1 |
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