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您的检索式：作者名="Gilbert Nshogoza"
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| 1 | Driving force of biomolecular liquid–liquid phase separation probed by nuclear magnetic resonance spectroscopy显示文摘The assembly of biomolecular condensates is driven by liquid–liquid phase separation. To understand the structure and functions of these condensates, it is essential to characterize the underlying driving forces, e.g., protein–protein and protein–RNA interactions. As both structured and low-complexity domains are involved in the phase separation process, NMR is probably the only technique that can be used to depict the binding topology and interaction modes for the structured and nonstructured domains simultaneously. Atomic-resolution analysis for the intramolecular and intermolecular interactions between any pair of components sheds light on the mechanism for phase separation and biomolecular condensate assembly and disassembly. Herein, we describe the procedures used for the most extensively employed NMR techniques to characterize key interactions for biomolecular phase separation. | Hanyu Zhang Weiwei Fan Gilbert Nshogoza Yaqian Liu Jia Gao Jihui Wu Yunyu Shi Xiaoming Tu Jiahai Zhang Ke Ruan | 2022 | Biophysics Reports2022，8，2： | 0 |
| 2 | 基于片段的核磁共振筛选方法识别NSD1 SET结构域的全新苗头化合物(英文)显示文摘包含SET结构域的核受体结合蛋白1(NSD1)是一种组蛋白甲基转移酶,它能够特异性的甲基化组蛋白H3赖氨酸第36位(H3K36).异常表达的NSD1主要发现于Sotos综合症患者体内,但它同样也能导致其他多种人类疾病的发生.目前已有靶向组蛋白甲基转移酶DOT1L和EZH2的小分子抑制剂报道,然而,靶向NSD1的化学探针分子尚未被发现.本文使用基于片段的核磁共振(NMR)筛选方法寻找到3个以NSD1蛋白作为靶点的苗头化合物,利用化学位移扰动分析技术测定了这些化合物与NSD1的结合亲和力.另外,利用分子对接方法选择获得苗头化合物与NSD1蛋白的最可能的结合模型.结果显示苗头化合物1结合于NSD1天然底物S-腺苷酸甲硫氨酸(SAM)的结合口袋中.我们的研究成果为进一步以结构为指导的从苗头化合物到先导化合物的衍化奠定了基础. | 汤衡 Gilbert NSHOGOZA 刘明清 刘亚茜 阮科 马荣声 高佳 | 2019 | 波谱学杂志2019，36，2： | 0 |
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