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| 1 | Virulence factors of Enterococcus strains isolated from patients with inflammatory bowel disease显示文摘AIM: To determine the features of Enterococcus that contribute to the development and maintenance of the inflammatory process in patients with inflammatory bowel disease (IBD). METHODS: Multiplex polymerase chain reaction (PCR) was applied to assess the presence of genes that encode virulence factors [surface aggregating protein (asa1), gelatinase (gelE), cytolysin (cylA), extracellular surface protein (esp) and hyaluronidase (hyl)] in the genomic DNA of 28 strains of Enterococcus isolated from the intestinal tissues of children with IBD (n =16) and of children without IBD (controls; n = 12). Additionally, strains with confirmed presence of the gelE gene were tested by PCR for the presence of quorum sensing genes (fsrA, fsrB, fsrC) that control the gelatinase production. Gelatinase activity was tested on agar plates containing 1.6% gelatin. We also analysed the ability of Enterococcus strains to release and decompose hydrogen peroxide (using Analytical Merckoquant peroxide test strips) and tested their ability to adhere to Caco-2 human gut epithelium cells and form biofilms in vitro. RESULTS: A comparison of the genomes of Enterococcus strains isolated from the inflamed mucosa of patients with IBD with those of the control group showed statistically significant differences in the frequency of theasa1 gene and thegelE gene. Furthermore, the cumulative occurrence of different virulence genes in the genome of a single strain ofEnterococcus isolated from the IBD patient group is greater than in a strain from the control group, although no significant difference was found. Statistically significant differences in the decomposition of hydrogen peroxide and adherence to the Caco-2 epithelial cell line between the strains from the patient group and control group were demonstrated. The results also showed that profuse biofilm production was more frequent amongEnterococcus strains isolated from children with IBD than in control strains. CONCLUSION: Enterococcus strains that adhere strongly to the intestinal epithelium, form biofilms and possess antioxidant defence mechanisms seem to have the greatest influence on the inflammatory process. | Edyta Golińska Anna Tomusiak Tomasz Gosiewski Gra yna Wiecek Agnieszka Machul Diana Mikoajczyk Magorzata Bulanda Piotr B Heczko Magdalena Strus | 2013 | World Journal of Gastroenterology2013，19，23： | 8 |
| 2 | Pt-Co nanostructures elec- trodeposited on graphene nanosheets for methanol electrooxidation 显示文摘 | OJANI R RAOOF J B GOLI M | 2014 | Journal of Power Sources2014，264，： | 1 |
| 3 | Sepcration and quantication of carotenoids in human plasma显示文摘 | KHACHIK F BEECHER G R GOLI M B | 1992 | Moth Enzymol1992，213，： | 1 |
| 4 | Tumour suppressor p53 inhibits human fibroblast growth factor 2 expression by a posttranscriptional mechanism显示文摘 | Goly B Creancier L Zanibellato C | 2001 | Oneogene2001，20，16： | 1 |
| 5 | Effect of food preparation on quality and quantative distribution of major carotenoid constituents of tomatoes and several green vegetables显示文摘 | Khachik F Goli M B Breecher G R | 1992 | J Agric Food Chem1992，40，： | 1 |
| 6 | Calculation of Rope Fatigue Strength on the Basis of Mechanical Proper-ties of Wires显示文摘 | Golis B Pilarczyk J W Blazejowski Z | 2005 | Wire Journal International2005，38，9： | 1 |
| 7 | Effect of food preparation on qualitative and quantitative distribution of major carotenoid constituents of tomatoes and several green vegetables 显示文摘 | Khachik F M Goli B | 1992 | J Agric Food Chem1992，56，： | 1 |
| 8 | Separation and identification of carotenoids and their oxidation products in extracts of human plasma显示文摘 | Khachik F Beecher G R Goli M B | 1992 | Anal Chem1992，64，： | 1 |
| 9 | Detection of apple stem pitting virus and pear vein yellows virus using reverse transcriptionpolymerase chain reaction显示文摘 | Malinowski I Komorowska B Golis T | 1998 | Acta Horticulturae1998，472，： | 1 |
| 10 | Separation and quantification of carotenoids in human plasma显示文摘 | KHACHIK F BEECHER G R GOLI M B | 1992 | Moth Enzymol1992，213，： | 1 |
| 11 | Blood leptin and C re- active protein provide more sensitive assessment than blood lipids and other inflammatory biomarkers in over- weight university students显示文摘 | Wang S Reed D B Goli S | 2011 | Nutrition Research2011，31，8： | 1 |
| 12 | Detection of apple stem pittng virus and pear vein yellow virus using reverse iranscription polymerase chain reaction 显示文摘 | Malinowski T Komorowska B Golis T | 1998 | Acta Hoticulturae1998，472，： | 1 |
| 13 | Calculation ofrope fatigue strength on the basis of mechanical properties of Wires 显示文摘 | Golis B Pilarczyk J W Blazejowski Z | 2005 | Wire Journal International2005，38，9： | 1 |
| 14 | Separation and identificationof carotenoids and their oxidation products in the extracts of humanplasma显示文摘 | KHACHIK F BEECHER G GOLI M B | 1992 | Analytical Chemistry1992，64，18： | 1 |
| 15 | Separation,identification,and quantification of carotenoids in fruits,vegetables and human plasma by high performance liquid chromatography显示文摘 | KHACHIK F BEECHER G R GOLI M B | 1991 | Pure and Appl Chem1991，63，1： | 1 |
| 16 | Effect of food preparation on qualitative and quantitative distribution of major carotenoidconstituents of tomatoes and several green vegetables显示文摘 | KHACHIK F GOLI M B BEECHER G R | 1992 | J Agric Food Chem1992，40，3： | 1 |
| 17 | Separation and quantitation of carotenoids in foods显示文摘 | KHACHIK F BEECHER G R GOLI M B | 1992 | Meth Enzymol1992，213，： | 1 |
| 18 | Effect of food preparation on qualitative and quantitative distribution of major carotenoid constitu- ents of tomatoes and several green vegetables显示文摘 | KHACHIK F GOLI M B BEECHER G | 1992 | Journal of Agricultural and Food Chemistry1992，40，： | 1 |
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