3篇
您的检索式：作者名="Gong Meijing"
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| 1 | Sliding modecontrol for uncertain neutral system with time~delays显示文摘 | Qu Shaocheng Gong Meijing Wang yongji | 2009 | Kybemetes2009，38，： | 1 |
| 2 | Chinese herbal compound Jinsiwei(金思维)improves synaptic plasticity in mice with sporadic Alzheimer’s disease induced by streptozotocin显示文摘OBJECTIVE:To investigate the efficacy of Jinsiwei(a patented Chinese herbal compound)on learning and memory impairment,the number of synapses and synaptic plasticity-related structural and functional protein expression in mice with sporadic Alzheimer’s disease(SAD)induced by streptozotocin.METHODS:Seventy-five C57/BL6J male mice were intracerebroventricularly injected with streptozotocin to establish the animal model of SAD.Mice were randomly divided into the model group(MG),donepezil group(DG),and the Jinsiwei high,medium,and low-dose groups(JH,JM,JL).Another fifteen C57/BL6J male mice were injected with artificial cerebrospinal fluid as the control group(CG).The intervention groups were intragastrically administrated with corresponding medicine,while the CG and MG were given 0.5%carboxymethyl cellulose by gavage.After 3 months,the Morris Water Maze test and step-down passive avoidance test were used to assess the learning and memory ability of mice.Synapses in hippocampal CA1 were observed by transmission electron microscope.Immunohistochemistry and western blotting were used to assess the distribution and expression levels of synaptic plasticity-related structural and functional proteins involving drebrin,cofilin,synapsin(syn),and N-methyl D-aspartate receptor subtype 2B(NR2B).RESULTS:The Morris Water Maze results showed that the escape latency in the Jinsiwei intervention groups was significantly shorter than that of the MG.Results of the step-down passive-avoidance test showed that the error times in the Jinsiwei intervention groups were significantly reduced compared with the MG.Transmission electron microscope results showed that the number of synapses in hippocampal CA1 was obviously increased in the Jinsiwei intervention groups compared with the MG.Immunohistochemical and western blotting results revealed that the positive cells and expression levels of drebrin,syn,and NR2B were significantly decreased in the MG and meanwhile cofilin significantly increased,while these changes were reversed after the Jinsiwei treatment.CONCLUSIONS:Jinsiwei can alleviate learning and memory impairments in a mouse model of SAD,increase the number of synapses and enhance synaptic plasticity via rescuing the expression of drebrin,syn,and NR2B and inhibiting cofilin expression. | TIAN Meijing HE Yannan ZHENG Mingcui QIN Gaofeng GONG Zhuoyan HUANG Shuaiyang WANG Pengwen | 2023 | Journal of Traditional Chinese Medicine2023，43，1： | 0 |
| 3 | Reading Time and DNA Sequence Preference of TET3 CXXC Domain Revealed by Single-Molecule Profiling显示文摘Recognition of CpG dinucleotide DNA in epigenetic information flow plays a pivotal role for cellular differentiation and development.The TET3 CXXC domain binds to CpG DNA,serving a basic epigenetic information reading mechanism.During the selective recognition of a CpG motif by a CXXC domain from crowded binding sites in a gene sequence,the protein-DNA interactions are beyond CpG dinu-cleotide.However,the selective binding dynamics of CpG within a long DNA context by epigenetic enzymes have been rarely exploit-ed,which is hard for ensemble methods to probe.Here,we used single-molecule magnetic tweezers to quantitatively examine the dynamics of TET3's CXXC domain on a Hoxa9 promoter DNA.Our single-molecule binding profile revealed that CXXC-DNA interactions involve both CpG motifs and their flanking sequences.The residence time of TET3 CXXC differs by about 1000 times in five distin-guished CpG clusters in the context of a CpG island.Moreover,we performed multi-state hidden Markov modeling analysis on the zip-ping/unzipping dynamics of a CpG hairpin,discovering TET3 CXXC's preference on CpG motifs regarding the-2 to+2 flanking bases.Our results shed light on the selective binding dynamics of a CXXC on a gene sequence,facilitating studies on epigenetic information reading mechanisms. | Zeyu Wang Zhiqiang Cao Kangkang Ma Man Lu Meije Wang Han Gao Deshun Gong Lin Liang Zhongbo Yu | 2023 | Chinese Journal of Chemistry2023，41，10： | 0 |
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