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| 1 | Pseudomonas fluorescens-like bacteria from the stomach:A microbiological and molecular study显示文摘AIM:To characterize oxidase-and urease-producing bacterial isolates,grown aerobically,that originated from antral biopsies of patients suffering from acid peptic diseases.METHODS:A total of 258 antral biopsy specimens were subjected to isolation of bacteria followed by tests for oxidase and urease production,acid tolerance and aerobic growth.The selected isolates were further characterized by molecular techniques viz.amplifications for 16S rRNA using universal eubacterial and HSP60 gene specific primers.The amplicons were subjected to restriction analysis and partial sequencing.A phylogenetic tree was generated using unweighted pair group method with arithmetic mean(UPGMA) from evolutionary distance computed with bootstrap test of phylogeny.Assessment of acidity tolerance of bacteria isolated from antrum was performed using hydrochloric acid from 10-7 mol/L to 10-1 mol/L.RESULTS:Of the 258 antral biopsy specimens collected from patients,179(69.4%) were positive for urease production by rapid urease test and 31%(80/258) yielded typical Helicobacter pylori(H.pylori) after 5-7 d of incubation under a microaerophilic environment.A total of 240(93%) antral biopsies yielded homogeneous semi-translucent and small colonies after overnight incubation.The partial 16S rRNA sequences revealed that the isolates had 99% similarity with Pseudomonas species.A phylogenetic tree on the basis of 16S rRNA sequences denoted that JQ927226 and JQ927227 were likely to be related to Pseudomonas fluorescens(P.fluorescens).On the basis ofHSP60 sequences applied to the UPGMA phylogenetic tree,it was observed that isolated strains in an aerobic environment were likely to be P.fluorescens,and HSP60 sequences had more discriminatory potential rather than 16S rRNA sequences.Interestingly,this bacterium was acid tolerant for hours at low pH.Further,a total of 250(96.9%) genomic DNA samples of 258 biopsy specimens and DNA from 240 bacterial isolates were positive for the 613 bp amplicons by targeting P.fluorescens-specific conserved putative outer membrane protein gene sequences.CONCLUSION:This study indicates that bacterial isolates from antral biopsies grown aerobically were P.fluorescens,and thus acid-tolerant bacteria other than H.pylori can also colonize the stomach and may be implicated in pathogenesis/protection. | Saurabh Kumar Patel Chandra Bhan Pratap Ajay Kumar Verma Ashok Kumar Jain Vinod Kumar Dixit Gopal Nath | 2013 | World Journal of Gastroenterology2013，19，7： | 4 |
| 2 | High-harmonic generation from spin-polarised defects in solids显示文摘The generation of high-order harmonics in gases enabled to probe the attosecond electron dynamics in atoms and molecules with unprecedented resolution.Extending these techniques to solids,which were originally developed for atomic and molecular gases,requires a fundamental understanding of the physics that has been partially addressed theoretically.Here,we employ timedependent density-functional theory to investigate how the electron dynamics resulting in high-harmonic emission in monolayer hexagonal boron nitride is affected by the presence of vacancies.We show how these realistic spin-polarised defects modify the harmonic emission and demonstrate that important differences exist between harmonics from a pristine solid and a defected solid.In particular,we found that the different spin channels are affected differently by the presence of the spin-polarised point defect.Moreover,the localisation of the wavefunction,the geometry of the defect,and the electron–electron interaction are all crucial ingredients to describe high-harmonic generation in defected solids. | M.S.Mrudul Nicolas Tancogne-Dejean Angel Rubio Gopal Dixit | 2020 | npj Computational Materials2020，，1： | 2 |
| 3 | Prevalence of Helicobacter pylori in asymptomatic subjects—A nested PCR based study显示文摘 | Shrutkirti Mishra Varsha Singh G.R.K. Rao V.K. Dixit A.K. Gulati Gopal Nath | 2008 | Infection Genetics and Evolution2008，，6： | 2 |
| 4 | Prevalence of Helicobacter pylori in asymptomatic subjects—A nested PCR based study显示文摘 | Shrutkirti Mishra Varsha Singh G.R.K. Rao V.K. Dixit A.K. Gulati Gopal Nath | 2008 | Infection Genetics and Evolution2008，，6： | 1 |
| 5 | Prevalence of Helicobacter pylori in asymptomatic subjects—A nested PCR based study显示文摘 | Shrutkirti Mishra Varsha Singh G.R.K. Rao V.K. Dixit A.K. Gulati Gopal Nath | 2008 | Infection, Genetics and Evolution2008，，6： | 1 |
| 6 | Four-dimensional imaging of lattice dynamics using ab-initio simulation显示文摘Time-resolved mapping of lattice dynamics in real-and momentum-space is essential to better understand several ubiquitous phenomena such as heat transport,displacive phase transition,thermal conductivity,and many more.In this regard,time-resolved diffraction and microscopy methods are employed to image the induced lattice dynamics within a pump–probe configuration.In this work,we demonstrate that inelastic scattering methods,with the aid of theoretical simulation,are competent to provide similar information as one could obtain from the time-resolved diffraction and imaging measurements.To illustrate the robustness of the proposed method,our simulated result of lattice dynamics in germanium is in excellent agreement with the time-resolved x-ray diffuse scattering measurement performed using x-ray free-electron laser.For a given inelastic scattering data in energy and momentum space,the proposed method is useful to image in-situ lattice dynamics under different environmental conditions of temperature,pressure,and magnetic field.Moreover,the technique will profoundly impact where time-resolved diffraction within the pump–probe setup is not feasible,for instance,in inelastic neutron scattering. | Navdeep Rana Aditya Prasad Roy Dipanshu Bansal Gopal Dixit | 2021 | npj Computational Materials2021，，1： | 0 |
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