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| 1 | Isolation and characterisation of human gingival margin-derived STRO-1/MACS^+ and MACS^-cell populations显示文摘Recently,gingival margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting(MACS) showed remarkable periodontal regenerative potential in vivo.As a second-stage investigation,the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive(MACS^+) and STRO-1-negative(MACS^-) cell populations from the human free gingival margin.Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies.Subsequently,the MACS^+ and MACS^- cell fractions were characterized by flow cytometry for expression of CD14,CD34,CD45,CD73,CD90,CD105,CD146/MUC18 and STRO-1.Colony-forming unit(CFU) and multilineage differentiation potential were assayed for both cell fractions.Mineralisation marker expression was examined using real-time polymerase chain reaction(PCR).MACS^+ and MACS- cell fractions showed plastic adherence.MACS^+ cells,in contrast to MACS- cells,showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs(P<0.01).More than 95%of MACS^+ cells expressed CD105,CD90 and CD73;lacked the haematopoietic markers CD45,CD34 and CD14,and expressed STRO-1 and CD146/MUC18.MACS- cells showed a different surface marker expression profile,with almost no expression of CD14 or STRO-1,and more than 95%of these cells expressed CD73,CD90 and CD146/MUC18,as well as the haematopoietic markers CD34 and CD45 and CD105.MACS^+ cells could be differentiated along osteoblastic,adipocytic and chondroblastic lineages.In contrast,MACS- cells demonstrated slight osteogenic potential.Unstimulated MACS^+ cells showed significantly higher expression of collagen I(P<0.05) and collagen III(P<0.01),whereas MACS^- cells demonstrated higher expression of osteonectin(P<0.05;MannWhitney).The present study is the first to compare gingival MACS^+ and MACS- cell populations demonstrating that MACS^+ cells,in contrast to MACS- cells,harbour stem/progenitor cell characteristics.This study also validates the effectiveness of the STRO-l/MACS^+technique for the isolation of gingival stem/progenitor cells.Human free gingival margin-derived STRO-1/MACS^+ cells are a unique renewable source of multipotent stem/progenitor cells. | Karim M Fawzy El-Sayed Sebastian Paris Christian Graetz Neemat Kassem Mohamed Mekhemar Hendrick Ungefroren Fred Fndrich Christof Drfer | 2015 | International Journal of Oral Science2015，7，2： | 6 |
| 2 | The assessment and monitoring of sparsely vegetated rangelands using calibrated Landsat data显示文摘 | Graetz R D Pech R R Davis A W | 1988 | International Journal of Remote Sensing1988，9，7： | 1 |
| 3 | An environmental threshold for degree of phosphorus saturation in sandy soils显示文摘 | Nair V D Portier K M Graetz D A | 2004 | Journal of Environmental Quality2004，33，： | 1 |
| 4 | Phosphorus retention by wetland soils used for treated wastewater disposal显示文摘 | Gale P M Reddy K R Graetz D A 1994 | 1994 | J Environ Qual1994，23，： | 1 |
| 5 | Determination of steroidal estrogens in flushed dairy manure wastewater by gas chromatography-mass spectrometry显示文摘 | HANSELMAN T A GRAETZ D A WILKIE A C | | 0，，03： | 1 |
| 6 | An environmental threshold for degree of phosphorus saturation in sandy soils 显示文摘 | Nair V D Portier K M Graetz D A | 2004 | Journal of Environmental Quality2004，33，： | 1 |
| 7 | The assessment and monitoring of sparsely vegetated rangelands using calibrated Landsat data显示文摘 | Graetz R D Pech R P Davis A W | 1988 | International Journal of Remote Sensing1988，9，： | 1 |
| 8 | Water table effects on phosphorus reactivity and mobility in a dairy manure-impacted Spodosol显示文摘 | VILLAPANDO R R GRAETZ D A | 2001 | Ecological Engineering2001，18，： | 1 |
| 9 | Phosphorus saturation in spodosols impacted by manure 显示文摘 | Nair V Graetz D | 2002 | Journal of Environmental Quality2002，31，： | 1 |
| 10 | Water table effects on histosol drainage water carbon,nitrogen and phosphorus显示文摘 | Martin H W Ivanoff D B Graetz D A | 1997 | Environmental Quality1997，，26： | 1 |
| 11 | Filtering Pathfinder AVHRR Land NDVI Data for Australia显示文摘 | Lovell J L Graetz R D | 2001 | International Journal of Remote Sensing2001，22，13： | 1 |
| 12 | The assessment and monitoring of sparsely vegetated rangelands of Absorbed landsat data显示文摘 | Graetz R D Pech R R Davis A W | 1988 | International Journal of Remote Sensing1988，9，7： | 1 |
| 13 | Influence of grazing management on vegetation,soil structure and nutrient distribution and the infiltration of applied rainfall in a semi-arid chenopod shrubland显示文摘 | GRAETZ R D TONGWAY D J | 1986 | Australian Jour-nal of Ecology1986，11，： | 1 |
| 14 | Manure-borne estrogens as potential environmental contaminants: A review 显示文摘 | Hanselman T A Graetz D A Wilkie A C | 2003 | Environmental Science and Technology2003，37，24： | 1 |
| 15 | 13C-depleted charcoal from C4 grasses and the role of occluded carbon in phytoliths 显示文摘 | KRULL E S SKJEMSTAD J O GRAETZ D | 2003 | Org Geochem2003，34，： | 1 |
| 16 | Filtering Pathfinder AVHRR LandNDVI Data for Australia显示文摘 | LOVELL J L GRAETZ R D | 2001 | International Journal of Remote Sens-ing2001，22，13： | 1 |
| 17 | Soil development in phosphate-mined created wetlands of Florida, USA显示文摘 | Nair V D Graetz D A Reddy K R | 2001 | Wetlands2001，21，2： | 1 |
| 18 | The charcoal sink of biomass burning on the Australian continent显示文摘 | Graetz R D Skjemstad J O | 2003 | CSIRO Atmospheric Research Technical paper2003，，64： | 1 |
| 19 | Phosphorus loss from organic versus inorganic fertilizers used in alleycropping on a Florida ultisol显示文摘 | Allen S C Nair V D Graetz D A | 2006 | Agriculture Ecosystems & Environment2006，117，： | 1 |
| 20 | Effects of allopurinol on in vivo suppression of arthritis in mice and ex vivo modulation of phagocytic production of oxygen radicals in whole human blood显示文摘 | Miesel R Zuber M Sanocka D Graetz R Kroeger H | 1994 | Inflammation1994，18，： | 1 |
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