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| 1 | Bifunctional chimeric SuperCD suicide gene -YCD: YUPRT fusion is highly effective in a rat hepatoma model显示文摘AIM: To investigate the effects of catalytically superior gene-directed enzyme prodrug therapy systems on a rat hepatoma model.METHODS: To increase hepatoma cell chemosensitivity for the prodrug 5-fluorocytosine (5-FC), we generated a chimeric bifunctional SuperCD suicide gene, a fusion of the yeast cytosine deaminase (YCD) and the yeast uracil phosphoribosyltransferase (YUPRT) gene.RESULTS: In vitro stably transduced Morris rat hepatoma cells (MH) expressing the bifunctional SuperCD suicide gene (MH SuperCD) showed a clearly marked enhancement in cell killing when incubated with 5-FC as compared with MH ceils stably expressing YCD solely (MH YCD) or the cytosine deaminase gene of bacterial origin(MH BCD), respectively. In vivo, MH SuperCD tumors implanted both subcutaneously as well as orthotopically into the livers of syngeneic ACI rats demonstrated significant tumor regressions (P<0.01) under both high dose as well as low dose systemic 5-FC application,whereas MH tumors without transgene expression (MH naive) showed rapid progression. For the first time, an order of in vivo suicide gene effectiveness (SuperCD>>YCD > > BCD > > > negative control) was defi ned as a result of a directin vivo comparison of all three suicide genes.CONCLUSION: Bifunctional SuperCD suicide gene expression is highly effective in a rat hepatoma model,thereby significantly improving both the therapeutic index and the efficacy of hepatocellular carcinoma killing by fluorocytosine. | Florian Graepler Marie-Luise Lemken Wolfgang A Wybranietz Ulrike Schmidt Irina Smirnow Christine D GroB Martin Spiegel Andrea Schenk Schenk Hansj(o|¨)rg Graf Ulrike A Lauer Reinhard Vonthein Michael Gregor Sorin Armeanu Michael Bitzer Ulrich M.Lauer | 2005 | World Journal of Gastroenterology2005，11，44： | 2 |
| 2 | CDMA/HDR: A bandwidth-efficient high-speed wireless data service for nomadic users 显示文摘 | P Bender P Black M Grob R Padovani N Sindhushayana A Viterbi | 2000 | IEEE Communication Magazine (S0733-8716)2000，38，7： | 1 |
| 3 | A Structural Model of Environmental Attitudes and Bc- haviour显示文摘 | Grob A | 1995 | Journal Of Environmental Psychology1995，，3： | 1 |
| 4 | Clinical experience with the Dynesys semirigid fixation system for the lumbar spine:surgical and patient-oriented outcome in 50 cases after an average of 2 years显示文摘 | Benini A Junge A | 2005 | J Spine2005，30，3： | 1 |
| 5 | Dabrafenib in BRAF -mutated metastatic melanoma : a multicentre, open- label, phase 3 randomised controlled trial显示文摘 | Hauschild A Grob JJ Demidov LV | 2012 | Lancet2012，380，9839： | 1 |
| 6 | A structural model of environmental attitudes and behavior显示文摘 | Grob A | 1995 | Journal of Environmental Psychology1995，，15： | 1 |
| 7 | Methods for determining the potential of acrylamide formation and its elimination in raw materials for food preparation, such as patatoes 显示文摘 | S Biedermann-Brem A Noti K Grob | 2002 | Mitteilungen Aus Lebens- mitteluntersuchung Und Hygiene2002，，93： | 1 |
| 8 | A structural model of environmental attitudes and behavior 显示文摘 | Grob A( 1995 ) | 1995 | Journal of Environmental Psychology1995，15，3： | 1 |
| 9 | Spark Decomposition of SF6_H2O Mixtures显示文摘 | Derdouri A Casanovas J Grob R | 1989 | IEEE Transactions on Electrical Insulation1989，24，6： | 1 |
| 10 | Ip- ilimnmab versus placebo after complete resection of stage III mela- noma: Initial efficacy and safety results from the EORTC 18071 phase trial显示文摘 | EGGERMONT A M CHIARION SILENI V GROB J J | 2014 | J Clin Oncol2014，32，18： | 1 |
| 11 | Clinical experience with the Dynesys semirigid fixation system for the lumbar spine:surgical and patient-oriented outcome in 50 cases after an average of 2years显示文摘 | Grob D Benini A Junge A | 2005 | Spine2005，30，3： | 1 |
| 12 | Clinical experience with the Dyne-sys semirigid fixation system for the lumbar spine: surgical and patient-oriented outcome in 50 cases after an average of 2 years 显示文摘 | Grob D Benini A Junge A | 2005 | Spine(Phila Pa 1976)2005，30，3： | 1 |
| 13 | Spark decomposition of SF6/H20 mixtures显示文摘 | Derdouri A Casanovas J Grob R | 1989 | IEEE Transactions on Electrical Insulation1989，24，9： | 1 |
| 14 | A structural model of environmental attitudes andbehaviour 显示文摘 | Grob A | 1995 | Journal of Environmental Psychology1995，15，3： | 1 |
| 15 | 显示文摘 | Mohanan S Grob A Herr U | 2006 | J Appl Phys2006，99，08： | 1 |
| 16 | The association between cervical spine curvature and neck pain显示文摘 | Grob D Frauenfelder H Mannion A F | 2007 | Eur Spine J2007，16，5： | 1 |
| 17 | Clinical experience with the dynesys semirigid fixation system for the lumbar spine surgical and patient oriented outcome in 50 cases after an average of 2 years显示文摘 | Grob D Benini A Junge A | | 0，，03： | 1 |
| 18 | Surgery for cervical stenosis: anterior cervical decompression, corpectomy, and fusion显示文摘 | Grob D Luca A | 2010 | Eur Spine J2010，19，： | 1 |
| 19 | Direct current electrical fields induce apoptosis in oral mucosa cancer cells by NADPH oxidase-derived reactive oxygen species 显示文摘 | Wartenberg M Wirtz N Grob A Niedermeier W Hescheler J Peters SC | 2008 | Bioelectromagnetics2008，29，1： | 1 |
| 20 | Human TFIID binds to core promoter DNA in a reorganized structural state显示文摘 | Cianfrocco M A Kassavetis G A Grob P | 2013 | Celi2013，152，2： | 1 |
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