|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Bifunctional chimeric SuperCD suicide gene -YCD: YUPRT fusion is highly effective in a rat hepatoma model显示文摘AIM: To investigate the effects of catalytically superior gene-directed enzyme prodrug therapy systems on a rat hepatoma model.METHODS: To increase hepatoma cell chemosensitivity for the prodrug 5-fluorocytosine (5-FC), we generated a chimeric bifunctional SuperCD suicide gene, a fusion of the yeast cytosine deaminase (YCD) and the yeast uracil phosphoribosyltransferase (YUPRT) gene.RESULTS: In vitro stably transduced Morris rat hepatoma cells (MH) expressing the bifunctional SuperCD suicide gene (MH SuperCD) showed a clearly marked enhancement in cell killing when incubated with 5-FC as compared with MH ceils stably expressing YCD solely (MH YCD) or the cytosine deaminase gene of bacterial origin(MH BCD), respectively. In vivo, MH SuperCD tumors implanted both subcutaneously as well as orthotopically into the livers of syngeneic ACI rats demonstrated significant tumor regressions (P<0.01) under both high dose as well as low dose systemic 5-FC application,whereas MH tumors without transgene expression (MH naive) showed rapid progression. For the first time, an order of in vivo suicide gene effectiveness (SuperCD>>YCD > > BCD > > > negative control) was defi ned as a result of a directin vivo comparison of all three suicide genes.CONCLUSION: Bifunctional SuperCD suicide gene expression is highly effective in a rat hepatoma model,thereby significantly improving both the therapeutic index and the efficacy of hepatocellular carcinoma killing by fluorocytosine. | Florian Graepler Marie-Luise Lemken Wolfgang A Wybranietz Ulrike Schmidt Irina Smirnow Christine D GroB Martin Spiegel Andrea Schenk Schenk Hansj(o|¨)rg Graf Ulrike A Lauer Reinhard Vonthein Michael Gregor Sorin Armeanu Michael Bitzer Ulrich M.Lauer | 2005 | World Journal of Gastroenterology2005，11，44： | 2 |
| 2 | Syk- and Card9-dependent coupling of innate immunity to the induction of T helper cells that produce interleukin 17显示文摘 | LeibundGut Landmann S Grob O Robinson MJ | 2007 | Nat Immunol2007，8，6： | 1 |
| 3 | Optimiza- tion of cellular nucleotide extraction and sample prepa- ration for nucleotide pool analysis using capillary elec- trophoresis显示文摘 | GROB M K O'BRIEN K CHU J J | 2003 | Journal of Chromatography B2003，788，： | 1 |
| 4 | Die innervation des sacroiliacalgelenkes beim menschen 显示文摘 | Grob K R Neuhuber W L Kissling R O | 1995 | Z Rheumatol1995，54，： | 1 |
| 5 | Contribution of picoplankton to the total particulate organic carbon concentration in the eastern South Pacific显示文摘 | GROB C ULLOA O CLAUSTRE H | 2007 | Biogeosciences2007，4，： | 1 |
| 6 | Influenza Virus-Susceptible Mice Carry Mx Genes with a Large Deletion or a Nonsense Mutation显示文摘 | STAEHELI P GROB R MEIER E SUTCLIFFE J G HALLER O | 1988 | Mol Cell Biol1988，8，10： | 1 |
| 7 | Rare oncogenic mutations of predictive markers for targeted therapy in triple-negative breast cancer显示文摘 | Grob TJ Heilenk(o)tter U Geist S | 2012 | Breast Cancer Res Treat2012，134，2： | 1 |
| 8 | Ipilimumab monotherapy in patients with pretreated advanced melanoma: a randomised, double-blind, multicentre, phase 2, dose-ranging study显示文摘 | Jedd D Wolchok Bart Neyns Gerald Linette Sylvie Negrier Jose Lutzky Luc Thomas William Waterfield Dirk Schadendorf Michael Smylie Troy Guthrie Jean-Jacques Grob Jason Chesney Kevin Chin Kun Chen Axel Hoos Steven J O’Day Celeste Lebbé | 2010 | Lancet Oncology2010，，2： | 1 |
| 9 | Matrix representation of quatemions显示文摘 | FAREBROTHER R W GROB J TROSCHKE S O | 2003 | Linear Algebra and its Applications2003，，362： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员