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您的检索式：作者名="Guixiang Zeng"
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| 1 | Diversity and high nitrogenase activity of endophytic diazotrophs isolated from Oryza rufipogon Griff.显示文摘Diversity and nitrogenase activity of endophytic diazotrophs colonized in the wild rice Oryza rufipogon Griff grown in Boluo, Huilai County in Guangdong Province and Lingshui County in Hainan Province were studied. Thirty-seven isolates obtained from Oryza rufipogon were identified as putative endophytic nitrogen-fixing bacteria by ARA (acetylene reduction assay) test and further confirmed by PCR amplification of nifH gene fragments. All obtained strains have ARA activity and the same sized nifH gene fragments. Above the similarity level of 80%, the obtained isolates were assigned as Group Ⅰ to Ⅷ by the clustering of IS-PCR fingerprints. The SDS-PAGE whole-cell protein patterns were similar to those of IS-PCR fingerprints. Components and contents of fatty acid methyl esters (FAMEs) were used to differentiate the representative strains (Ls13, Ls8, BL1, BL12, HL6, Ls4) from Group Ⅰ to Group Ⅵ. The six representative strains showed significant difference in contents and components of cellular fatty acid methyl ester. 16S rDNA sequencing analysis showed that strains of Group Ⅰ to Ⅶ were located in Enterobacteraceae (γ-proteobacteria). Strains of Group Ⅰ and Group II were closely related to Klebsiella sp.; Strain Ls8 of Group Ⅱ was a little far away from the genus of Pantoea (homology level 96% with Pantoea agglomerans), which may represent a new species or genus in Enterobacteraceae; Strains of Groups IV and V belonged to different Enterobacter sp.; Strain Ls4 and Ls 9 representing Group Ⅵ were close to Citrobacter amalonaticus with 98% sequence similarity; Strain Ls15 of Group Ⅶ showed 98% sequence identity with Pantoea sp.; Strains of Group Ⅷ were assigned to the genus Ideonella (β-proteobacteria). Based on the above results, endophytic diazotrophs isolated from O. rufipogon showed great diversity and some diazotrophs showed high nitrogenase activity with 42.52 μmol/mL·h C2H4. Inoculation to rice tests indicated that the isolated endophytic diazotrophs significantly promoted the rice growth. | TAN ZhiYuan PENG GuiXiang XU PeiZhi AI ShaoYing TANG ShuanHu ZHANG GuoXia ZENG FengYun | 2009 | Chinese Science Bulletin2009，54，16： | 14 |
| 2 | Simultaneous determination of nine fluoro-quinolonesin egg white and egg yolk by liquid chromatography withfluorescence detection显示文摘 | Zhenling Zeng Aiguo Dong Guixiang Yang | 2005 | Journal of ChromatographyB2005，821，： | 1 |
| 3 | Simultaneous determination of nine fluoroquinolones in egg white and egg yolk by liquid chromatography with fluorescence detection 显示文摘 | ZENG Zhenling DONG Aiguo YANG Guixiang | 2005 | J Chromatogr B2005，821，2： | 1 |
| 4 | Carbon-halogen bond activation by a structurally constrained phosphorus(Ⅲ)platform显示文摘Theσ-bond activation by main group element has received enormous attention from theoretical and experimental chemists.Here,the reaction of C-X(X=Cl,Br,I)bonds in benzyl and allyl halides with a pincer-type phosphorus(Ⅲ)species was reported.A series of structurally robust phosphorus(Ⅴ)compounds were formed via the formal oxidative addition reactions of C-X bonds to the phosphorus(Ⅲ)center.Density functional theory calculations show that the nucleophilic addition process is more favorable than the direct oxidative addition mechanism.Isomerization of bent structures of phosphorus(Ⅲ)compound to poorly nucleophilic compounds to undergo further C-X bond activation can be rationalized by frontier molecule orbital analysis.This study not only provides a deep understanding of the reactivity of phosphorus(Ⅲ)species but also demonstrates a potential of main group elements for the small-molecule activation. | Penglong Wang Qin Zhu Yi Wang Guixiang Zeng Jun Zhu Congqing Zhu | 2021 | Chinese Chemical Letters2021，32，4： | 0 |
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