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| 1 | Recent progress on lanthanide complexes/clay minerals hybrid luminescent materials显示文摘The unique luminescent performance of lanthanide complexes/clay minerals hybrid materials has been fascinating many researchers for recent decades.It not only retains the excellent luminescent characteristics of lanthanide complexes but also improves the poor stability of the complexes.In this article,we introduce the luminescence mechanism of lanthanide complexes and point out the necessity of their combination with clay minerals.After the analysis of the structure and interlayer environment differences of 1:1-type and 2:1-type clay minerals,the intercalation methods(covalent grafting and ion exchange)appropriate for different clay minerals are summarized with examples.Based on the luminescence characteristics of the hybrid materials,the applications of these materials as luminescent probes in recognition of specific metal cations and molecules,detection of pH value,and temperature are reviewed.Finally,the current problems in the preparation of lanthanide complexes/clay minerals hybrid luminescent materials and shortcomings that need improvement in their performance are analyzed,and the application prospect is forecast. | Ga Zhang Lefu Mei Junjie Ding Ke Su Qingfeng Guo Guocheng Lv Libing Liao | 2022 | Journal of Rare Earths2022，40，9： | 2 |
| 2 | Metabolic engineering of Escherichia coli for the production of Lacto-N-neotetraose(LNnT)显示文摘Lacto-N-neotetraose(LNnT),one of the most important human milk oligosaccharides,can be used as infants’food addi-tives.Nowadays,extraction,chemical and biological synthesis were utilized to obtain LNnT,while these methods still face some problems such as low yield and high cost.The aim of current work is to construct a de novo biosynthesis pathway of LNnT in E.coli K12 MG1655.The lgtA and lgtB were first expressed by a plasmid,resulting in a LNnT titer of 0.04 g/L.To improve the yield of LNnT on substrate lactose,lacZ and lacI were knocked out,and lacY was over-expressed.As a result,the yield of LNnT on lactose increased from 0.01 to 0.09 mol/mol,and the titer of LNnT elevated to 0.41 g/L.In addition,the pathway was regulated using the titer of Lacto-N-triose II(LNTII)as a measure,and obtained a high titer strain of LNnT for 1.04 g/L.Finally,the gene expressions were fine-tuned,the titer of LNnT reached 1.2 g/L,which was 93%higher than the control strain,and the yield on lactose reached 0.28 mol/mol.The engineering strategy of pathway construction and modulation used in this study is applicable to facilitate the microbial production of other metabolites in E.coli. | Wei Zhang Zhenmin Liu Mengyue Gong Nan Li Xueqin Lv Xiaomin Dong Yanfeng Liu Jianghua Li Guocheng Du Long Liu | 2021 | Systems Microbiology and Biomanufacturing2021，1，3： | 2 |
| 3 | Recent advances and challenges in microbial production of human milk oligosaccharides显示文摘Human milk oligosaccharides(HMOs)are one of the major differences between livestock milk and human milk,and the prebiotic functions of HMOs have been verified through in vitro and clinical trials.The most abundant HMOs include 2′-fucysollactose(2′-FL),3-fucosyllactose(3-FL),lacto-N-neotetraose(LNnT)and lacto-N-tetraose(LNT);their application and synthesis have attracted wide attentions.In recent years,the biotechnological production of 2′-FL,3-FL,LNnT and LNT have emerged based on techniques such as whole-cell catalysis and fermentation.In particular,the development of metabolic engineering and synthetic biology methods and strategies have facilitated efficient biosynthesis of these HMOs.However,these advantages have not been systematically reviewed yet.In this review,we first discuss the structures and applications of HMOs;secondly,strategies of microbial synthesis of the most abundant 2′-FL,3-FL,LNnT and LNT are summarized and compared.Finally,challenges and perspectives of efficient microbial production of HMOs as well as strategies for overcoming the challenges are discussed.This review reveals the whole picture of recent development in HMOs microbial synthesis and can further facilitate the understanding of limiting factors,and further propose a few directions to promote the development of efficient production hosts. | Jieying Deng Xueqin Lv Jianghua Li Guocheng Du Jian Chen Long Liu | 2021 | Systems Microbiology and Biomanufacturing2021，1，1： | 2 |
| 4 | Current advances in design and engineering strategies of industrial enzymes显示文摘Enzymes are considered as functional proteins,also known as biocatalysts,which are required for normal cellular function.The commercial production of enzymes with distinct features always remains the top priority of researchers for industrial biocatalysis.However,the insufficient yield,low stability,low activity,formation of by-products,complex purification pro-cess,and many other factors are challenging for the enzyme production industry.Novel advancements in protein engineering,especially the combination of multidisciplinary techniques including post-translational enzyme modification,structured assisted protein tailoring and computational modeling approaches,open a new horizon to more efficient production of bio-catalysts.In this review,we focus on the development process of enzyme modification design strategies,such as rational design,semi-rational design and de novo design.Selected examples for each strategy,which are particularly useful for novel enzyme design,are given.We hope this review is helpful for the development of biocatalysts for industrial application. | Tanatarov Dinmukhamed Ziyang Huang Yanfeng Liu Xueqin Lv Jianghua Li Guocheng Du Long Liu | 2021 | Systems Microbiology and Biomanufacturing2021，1，1： | 2 |
| 5 | Formation processes of CuCl and regenerated Cu crystals on bronze surfaces in neutral and acidic media显示文摘 | Julin Wang Chunchun Xu Guocheng Lv | 2006 | Applied Surface Science2006，252，： | 1 |
| 6 | Influence of Interlayer Cations on Organic Intercalation of Montmorillonite显示文摘 | Wu Limei Liao Libing Lv Guocheng | 2015 | Journal of Colloid and Interface Science2015，454，： | 1 |
| 7 | A review on current conventional and biotechnical approaches to enhance biosynthesis of steviol glycosides in Stevia rebaudiana显示文摘Stevia rebaudiana Bertoni is commonly called stevia and mostly found in the north east regions of South America.It is an herbaceous and shrubby plant belonging to the Asteraceae family.Stevia is considered as a natural sweetener and a commercially important plant worldwide.The leaves of S.rebaudiana contain steviol glycosides(SGs)which are highly potent and non-caloric sweeteners.The sweetening property of S.rebaudiana is contributed to the presence of these high potency,calorie free steviol glycosides.SGs are considerably suitable for replacing sucrose and other artificial sweetening agents which are used in different industries and pharmaceuticals.SGs amount in the plant mostly varies from 8%to 10%,and the enhancement of SGs is always in demand.These glycosides have the potential to become healthier alternatives to other table sugars for having desirable taste and zero calories.SGs are almost 300 times sweeter than sucrose.Being used as alternative sugar intensifier the commercial value of this plant in biopharmaceutical,food and beverages industries and in international market is increasing day by day.SGs have made stevia an important part of the medicinal world as well as the food and beverage industry,but the limited production of plant material is not fulfilling the higher global market demand.Therefore,researchers are working worldwide to increase the production of important SGs through the intercession of different biotechnological approaches in S.rebaudiana.This review aims to describe the emerging biotechnological strategies and approaches to understand,stimulate and enhance biosynthesis of secondary metabolites in stevia.Conventional and biotechnological methods for the production of steviol glycosides have been briefly reviewed and discussed. | Samra Basharat Ziyang Huang Mengyue Gong Xueqin Lv Aqsa Ahmed Iftikhar Hussain Jianghua Li Guocheng Du Long Liu | 2021 | Chinese Journal of Chemical Engineering2021，34，2： | 1 |
| 8 | Micro-electrolysis of Cr (VI) in the nanoscale zero-valent iron loaded activated carbon 显示文摘 | Wu Limei Liao Libing Lv Guocheng | 2013 | Journal of Hazardous Materials2013，，： | 1 |
| 9 | Metabolic engineering of Corynebacterium glutamicum S9114 based on whole-genome sequencing for efficient N-acetylglucosamine synthesis显示文摘Glucosamine(GlcN)and its acetylated derivative N-acetylglucosamine(GlcNAc)are widely used in the pharmaceutical industries.Here,we attempted to achieve efficient production of GlcNAc via genomic engineering of Corynebacterium glutamicum.Specifically,we ligated the GNA1 gene,which converts GlcN-6-phosphate to GlcNAc-6-phosphate by transferring the acetyl group in Acetyl-CoA to the amino group of GlcN-6-phosphate,into the plasmid pJYW4 and then transformed this recombinant vector into the C.glutamicum ATCC 13032,ATCC 13869,ATCC 14067,and S9114 strains,and we assessed the GlcNAc titers at 0.5 g/L,1.2 g/L,0.8 g/L,and 3.1 g/L from each strain,respectively.This suggested that there were likely to be significant differences among the key genes in the glutamate and GlcNAc synthesis pathways of these C.glutamicum strains.Therefore,we performed whole genome sequencing of the S9114 strain,which has not been previously published,and found that there are many differences among the genes in the glutamate and GlcNAc synthesis pathways among the four strains tested.Next,nagA(encoding GlcNAc-6-phosphate deacetylase)and gamA(encoding GlcN-6-phosphate deaminase)were deleted in C.glutamicum S9114 to block the catabolism of intracellular GlcNAc,leading to a 54.8%increase in GlcNAc production(from 3.1 to 4.8 g/L)when grown in a shaker flask.In addition,lactate synthesis was blocked by knockout of ldh(encoding lactate dehydrogenase);thus,further increasing the GlcNAc titer to 5.4 g/L.Finally,we added a key gene of the GlcN synthetic pathway,glmS,from different sources into the expression vector pJYW-4-ceN,and the resulting recombinant strain CGGN2-GNA1-CgglmS produced the GlcNAc titer of 6.9 g/L.This is the first report concerning the metabolic engineering of C.glutamicum,and the results of this study provide a good starting point for further metabolic engineering to achieve industrial-scale production of GlcNAc. | Chen Deng Xueqin Lv Yanfeng Liu Jianghua Li Wei Lu Guocheng Du Long Liu | 2019 | Synthetic and Systems Biotechnology2019，4，3： | 0 |
| 10 | In silico cell factory design driven by comprehensive genome‑scale metabolic models:development and challenges显示文摘Genome-scale metabolic models(GEMs)have been widely used to design cell factories in silico.However,initial flux balance analysis only considers stoichiometry and reaction direction constraints,so it cannot accurately describe the distribution of metabolic flux under the control of various regulatory mechanisms.In the recent years,by introducing enzymology,thermodynamics,and other multiomics-based constraints into GEMs,the metabolic state of cells under different conditions was more accurately simulated and a series of algorithms have been presented for microbial phenotypic analysis.Herein,the development of multiconstrained GEMs was reviewed by taking the constraints of enzyme kinetics,thermodynamics,and transcriptional regulatory mechanisms as examples.This review focused on introducing and summarizing GEMs application tools and cases in cell factory design.The challenges and prospects of GEMs development were also discussed. | Jiangong Lu Xinyu Bi Yanfeng Liu Xueqin Lv Jianghua Li Guocheng Du Long Liu | 2023 | Systems Microbiology and Biomanufacturing2023，3，2： | 0 |
| 11 | Chevrel phase:A review of its crystal structure and electrochemical properties显示文摘Chevrel phase compounds have attracted increasing attention as electrochemical energy storage materials and electro-catalysts.Benefiting from their unique crystal structure,Chevrel phase compounds can not only function as the host structures for reversible intercalation of a broad range of cations,but also exhibit high catalytic activity in electrochemical reduction reactions.Here we provide an overview of recent progress in the development of Chevrel phase materials including new understanding of structural features,synthetic methods,and electro-chemical properties.A brief conclusion and perspectives on the future development of Chevrel phase materials are also provided. | Meng Liu Guocheng Lv Tianming Liu Hao Liu Lingchang Kong Lintao Tian Wenxiu Rao Yuxin Li Libing Liao Juchen Guo | 2023 | Progress in Natural Science:Materials International2023，33，1： | 0 |
| 12 | Pt/Mo chalcogenide composite deriving from Pt-Mo_(6)S_8 by high temperature shock for enhanced HER performance显示文摘Highly efficient catalysts for electrolysis of water are crucial to the development of hydrogen energy which is helpful to carbon neutralization.Recently,high temperature shock(HTS),with advantage of rapid speed,universality and scalable production,has been a promising method in synthesis of nanomaterials.In this paper,HST was used to treat low Pt loading Mo_(6)S_(8)for enhanced water splitting performance.Impressively,the optimized MoS_(2)/MoO_(2)/Mo_(6)S_(8)nano-composite with low Pt mass loading(~4%)displays well hydrogen evolution reaction(HER)electrochemical performance.The overpotential is 124 mV to reach 10 mA/cm^(2)and the corresponding Tafel slope is 88 mV/dec in acidic electrolyte.Its mass activity is 6.2 mA/μg_(Pt)at-124 mV vs.RHE,which is almost 2 times relative to 20%Pt/C.Moreover,it presents distinguished stability even after 2000 cycles.This work will broaden the way of catalysts preparation and the application of hydrogen evolution. | Meng Liu Guocheng Lv Hao Liu Tianming Liu Lingchang Kong Libing Liao | 2024 | Chinese Chemical Letters2024，35，3： | 0 |
| 13 | Remodeling Isoprene Pyrophosphate Metabolism for Promoting Terpenoids Bioproduction显示文摘Terpenoids are the largest family of natural products.They are made from the building block isoprene pyrophosphate(IPP),and their bioproduction using engineered cell factories has received a great deal of attention.To date,the insufficient metabolic supply of IPP remains a great challenge for the efficient synthesis of terpenoids.In this work,we discover that the imbalanced metabolic flux distribution between the central metabolism and the IPP supply hinders IPP accumulation in Bacillus subtilis(B.subtilis).Therefore,we remodel the IPP metabolism using a series of genetically encoded two-input-multioutput(TIMO)circuits that are responsive to pyruvate or/and malonyl-CoA,resulting in an IPP pool that is significantly increased by up to four-fold.As a proof-of-concept validation,we design an IPP metabolism remodeling strategy to improve the production of three valuable terpenoids,including menaquinone-7(MK-7,4.1-fold),lycopene(9-fold),andβ-carotene(0.9-fold).In particular,the titer of MK-7 in a 50-L bioreactor reached 1549.6 mg·L^(-1),representing the highest titer reported so far.Thus,we propose a TIMO genetic circuits-assisted IPP metabolism remodeling framework that can be generally used for the synergistic fine-tuning of complicated metabolic modules to achieve the efficient bioproduction of terpenoids. | Xianhao Xu Xueqin Lv Shixiu Cui Yanfeng Liu Hongzhi Xia Jianghua Li Guocheng Du Zhaofeng Li Rodrigo Ledesma-Amaro Jian Chen Long Liu | 2023 | Engineering2023，，9： | 0 |
| 14 | Enzymatic production of N‑acetylneuraminic acid:advances and perspectives显示文摘N-Acetyl-d-neuraminic acid(NeuAc),a well-known and well-studied sialic acid,is found in cell surface glycolipids and glycoproteins,where it performs a variety of biological functions.The use of NeuAc as a nutraceutical for infant brain development and as an intermediate for pharmaceutical production demands its production on an industrial scale.Natural extraction,chemical synthesis,enzymatic synthesis,and biosynthesis are the methods used for NeuAc production.Among these methods,enzymatic synthesis using N-acetyl-glucosamine(GlcNAc)2-epimerase(AGE)for epimerization and N-acetyld-neuraminic acid lyase(NAL)for aldol condensation,has been reported to produce NeuAc with high production efciency.In this review,we discuss advances in the two-step enzymatic synthesis of NeuAc using pyruvate and GlcNAc as substrates.The major challenges in producing NeuAc with high yield are highlighted,including multiple parameter-dependent processes,undesirable reversibility,and diminished solubility of AGEs and NALs.Further,diferent strategies applied to overcome the limitations of the two-step enzymatic production are discussed,such as pyruvate concentration and temperature shift during the process to increase conversion yield,use of mathematical and computational simulations for process optimization,enzyme engineering to make enzymes highly efcient,and the use of tags and chaperones to increase enzyme solubility.We suggest future directions and the strategies that can be followed to improve enzymatic synthesis of NeuAc. | Muhammad Iftikhar Hussain Xiaolong Zhang Xueqin Lv Samra Basharat Umar Shahbaz Jianghua Li Guocheng Du Long Liu Yanfeng Liu | 2022 | Systems Microbiology and Biomanufacturing2022，2，1： | 0 |
| 15 | Chitin deacetylase:from molecular structure to practical applications显示文摘Chitosan and chitooligosaccharides(COS),as derivatives of chitin through deacetylation reaction,have broad applications due to their good biodegradability,biocompatibility,and solubility.In addition,chitosan and COS are involved in cell wall morphogenesis and host–pathogen interactions in vivo.Chitin deacetylases(CDAs)are enzymes that can catalyze the deN-acetylation of chitin.They are widely distributed in protozoa,algae,bacteria,fungi,and insects with important physiological functions.Compared with the traditional chemical method,enzymatic catalysis by CDAs provides an enzymatic catalysis method to produce chitosan and COS with controllable deacetylation site and environmental friendliness.These characteristics attract researchers to produce CDAs by fungicides or pesticides.However,researches on heterologous expression and directed evolution of CDAs are still lacking.In this review,we summarize the latest knowledge of CDAs,especially for heterologous expression systems and directed evolution strategies,which may contribute to the industrial production and future application of CDAs. | Ziyang Huang Xueqin Lv Guoyun Sun Xinzhu Mao Wei Lu Yanfeng Liu Jianghua Li Guocheng Du Long Liu | 2022 | Systems Microbiology and Biomanufacturing2022，2，2： | 0 |
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