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| 1 | Human embryonic stem cells-derived endothelial cell therapy facilitates kidney regeneration by stimulating renal resident stem cell proliferation in acute kidney injury显示文摘Endothelial cell therapy has been implicated to enhance tissue regeneration and vascularization in ischemic kidney. However, no published study has yet examined direct effects of endothelial cell treatment in kidney recovery. This study investigated the therapeutic efficacy of endothelial cells in a mouse model with acute kidney injury (AKI). Thus, human embryonic stem cells-derived endothelial cells (hESC-ECs) labeled with a reporter system encoding a double fusion reporter gene for firefly luciferase (Fluc) and green fluorescent protein (GFP) were characterized by Fluc imaging and immunofluoresence staining. Cultured hESC-ECs (1×106) were injected into ischemic kidney shortly after AKI. Survival of the transplanted hESC-ECs was monitored in vivo from day 1 to 14 after endothelial cell transplantation and potential impact of hESC-EC treatment on renal regeneration was assessed by histological analyses. We report that a substantial level of bioluminescence activity was detected 24 h after hESC-EC injection followed by a gradual decline from 1 to 14 d. Human ESC-ECs markedly accelerated kidney cell proliferation in response to ischaemia-induced damage, indicated by an elevated number of BrdU+ cells. Co-expression of Sca-1, a kidney stem cell proliferation marker, and BrdU further suggested that the observed stimulation in renal cell regeneration was, at least in part, due to increased proliferation of renal resident stem cells especially within the medullary cords and arteriole. Differentiation of hESC-ECs to smooth muscle cells was also observed at an early stage of kidney recovery. In summary, our results suggest that endothelial cell therapy facilitates kidney recovery by promoting vascularization, trans-differentiation and endogenous renal stem cell proliferation in AKI. | JIA XiaoHua L He LI Chen FENG GuoWei YAO XinPeng MAO LiNa KE TingYu CHE YongZhe XU Yong LI ZongJin KONG DeLing | 2013 | Chinese Science Bulletin2013，58，23： | 4 |
| 2 | Dual innervation of spinal ganglion neurons on perineum and bladder显示文摘Experiments were performed on 13 Wister rats. Fastblus (FB) was injected into subcutaneous tissue in perineum area and nuclear yellow (NY) was injected into subserous lamina of the bladder wall, respectively. FB, NY and FB+NY were mostly found in the spinal ganglions of L6, S1 ans S2 segments. The numbers of FB,NY and FB+NY labeled cells were 146, 186 and 81, in a total of 463 labeled cells and their proportion was 40%, 51% and 9%, respectively. The result indicates that the spinal ganglion neurons dually innervate both the somatic and visceral tissues, and the convergence of somato_visceral sensory pathways might occur in the spinal ganglion cells. | Guowei Lü Zhuo Meng Baohong Li Zhigao Jin | 1998 | Chinese Science Bulletin1998，43，2： | 3 |
| 3 | Can we be aware of both visceral and somatic sensations via a single neuronal pathway?显示文摘Sensory information arising from somatic and visceral area is thought to be respectively transmitted to the brain by two distinct sensory neuronal systems, the somatic and visceral sensory pathway. A novel and unique spinal sensory system, the spinosolitary tract-dorsal column postsynaptic neuronal system (SST-DCPS), was physiologically and anatomically identified. The spinal neurons project to both visceral (the solitary tract nucleus) and somatic sensory (the dorsal column nuclei) nuclei via their branched axons and receive both visceral and somatic sensory information ascending from periphery through dichotomized primary afferents and descending from their targets via their branched axons. The brain might thus be aware of both visceral and somatic sensation via a single SST-DCPS neuronal channel. The finding of SST-DCPS system, as an example, might be considered as an intersection or fuzzy set of the SST and DCPS system and the concept of dichotomy in classification of neurons and neuronal pathways | Guowei Lü Qingjin Li Zhuo Meng Xiaohong Liu | 2002 | Chinese Science Bulletin2002，47，23： | 2 |
| 4 | Understanding employees' willingness to contribute to shared electronic databases显示文摘 | Jian Guowei Jeffres L W | 2006 | Communication Research2006，33，4： | 1 |
| 5 | Effects of splenomegaly and splenic macrophage activity in hypersplenism due to cirrhosis显示文摘 | Yongxiang W Zongfang L Guowei L | 2002 | Am J Med2002，113，： | 1 |
| 6 | Effect of dietary proteinlevel and origin on the redox status显示文摘 | CHUNMEI G YONGHUI S GUOWEI L | 2008 | Int J Mol Sci2008，9，： | 1 |
| 7 | Mesozoic basin develop- ment and tectonic evolution of the Dabieshan orogenic belt, central China 显示文摘 | Liu Shaofeng Heller P L Zhang Guowei | 2003 | Tectonics2003，22，4： | 1 |
| 8 | Oldenlandia diffusaextracts exert antiproliferative and apoptotic effects on human breastcancer cells through ERa /Spl-mediated p53 activation 显示文摘 | Gu Guowei Barone I Gelsomino L | 2012 | J CellPhysiol2012，227，10： | 1 |
| 9 | Effect of Dietary Protein Level and Origin on the Redox Status显示文摘 | Chunmei G Yonghui S Guowei L | 2008 | Int J Mol Sci2008，9，： | 1 |
| 10 | Jolkinolide B induces neuroen-docrine differentiation of human prostate LNCaP cancer cell line显示文摘 | W K L J C K H Guowei Q | 2002 | Biochem Pharm2002，63，： | 1 |
| 11 | Reclaimed municipal waste water-a potential water resouce in China显示文摘 | He Pingjing Phan L Gu Guowei | 2001 | Water Science and Technology2001，43，10： | 1 |
| 12 | Symmetry-breaking Subharmonic Bifurcationsof Periodicaly Parameter-excited Systems显示文摘Symmetry-breakingSubharmonicBifurcationsofPeriodicalyParameter-excitedSystems11ThepaperwasreceivedonAug.30th,1996GuoweiHE1Lab... | Guowei HE 1 Laboratory for Nonlinear Mechanics, Institute of Mechanics, Chinese Academy of Science, Beijing, 10080, China 2 Service d’Astrophysique, CE-Saclay, L’orme des Merisiers 709, 91191, Gif sur Yvette, France E-mail: he@discovery | 1996 | Communications in Nonlinear Science and Numerical Simulation1996，1，4： | 0 |
| 13 | 左旋精氨酸对大鼠心脏移植模型移植物血管病变的抑制作用(英文)显示文摘目的 研究左旋精氨酸对心脏移植物血管病变的抑制作用及其机制。方法 采用大鼠异位心脏移植模型。对照组 2 6只 ,移植后不用左旋精氨酸 ;实验组 2 1只 ,移植后按每天 80 0mg kg将左旋精氨酸加入饮水中。于移植后 2个月和 3个月检测各组的心脏移植物血管病变评分和血浆一氧化氮含量。结果 移植后 2个月 ,实验组的移植物存活率为 90 .5 % ,显著高于对照组的 6 1.5 % (P <0 .0 5 )。移植后 2个月和 3个月 ,实验组血浆一氧化氮均显著高于对照组 ,分别为 (10 5 .37± 10 .6 6 ) μmol Lvs (6 8.5 4± 6 .83) μmol L(P <0 .0 5 ) ,和 (10 4 .5 3± 12 .31) μmol Lvs(6 6 .32± 10 .5 4 ) μmol L(P <0 .0 5 ) ;而对照组心脏移植物血管病变评分显著高于实验组 ,分别为 2 .4± 0 .7vs 1.1±0 .6 (P <0 .0 5 ) ,和 3.0± 0 .8vs 1.6± 0 .9(P <0 .0 5 )。实验组心脏移植物的冠状动脉内膜病变轻微 ,内皮和内弹力层基本保持完整 ,平滑肌细胞增殖不明显。结论 补充左旋精氨酸可改善心脏移植物血管病变 ,其机制与一氧化氮合成增加有关。一氧化氮具有保护内皮功能 ,抑制平滑肌细胞增殖的作用。 | 张庆华 姚志发 刘宏宇 LI Jun-quan Lü Hang ZHANG Guowei | 2004 | 哈尔滨医科大学学报2004，38，5： | 0 |
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