| 1 | Evaluating the reliability of microsatellite genotyping from low-quality DNA templates with a polynomial distribution model显示文摘Molecular studies using trace DNA, such as from museum specimens, ancient or forensic samples and samples obtained noninvasively, often have a common problem of low quality of DNA templates. Amplification errors, such as allelic dropout and false allele, may arise during polymerase chain reaction (PCR) using such samples. A mathematical model which treats homozygotes and heterozygotes discriminately has been developed to measure sample quality and compute the confidence level of using multipletube approaches. We use plucked hair samples collected from 26 individual Sichuan snub-nosed monkeys (Rhinopithecus roxel- lana) to test the model. In this case, a confidence level of 99% can be achieved by three positive PCRs. If the sample quality is very poor and requires many PCR replicates, an alternative multiplestep genotyping method is recommended. This model enables researchers to optimize experimental protocols through pilot studies and obtain reliable genetic information using noninvasive sampling method. | HE Gangi HUANG Kang GUO SongTao JI WeiHong QI XiaoGuang REN Yi JIN XueLin LI BaoGuo | 2011 | Chinese Science Bulletin2011，56，24： | 8 |
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