| 1 | Private Car Travel Characteristics and Influencing Factors in Chinese Cities——A Case Study of Guangzhou in Guangdong,China显示文摘Taking Guangzhou as a case,this paper adopted a questionnaire survey to gather first-hand data and analyzed the characteristics and influencing factors of private car travel in Chinese cities.As the research indicated,trip purposes of private car travel are mainly commute and business affairs with a more flexible trip in the urban core area.And trip intensities are concentrated in a certain extent,with trip frequency being lower in the urban core area than the peripheral area.In addition,the trip time has two significant peaks occurring in the morning and afternoon,and one trough in the midday.And trip spatial distribution is mainly within commute with both residence and employment in urban area and inward commute with residence in suburban area while employment in urban area.Both kinds of commutes direct to the urban area.The study also shows that the characteristics of private car travel are principally influenced by two aspects:travelers' attributes and urban characteristics.The main travelers' social and economic attributes influenced it include the gender,education attainment,age,driving experience and per capita monthly household income.The urban characteristics influenced it mainly cover the land use pattern,public traffic facilities and spatial attributes of residential environment. | CAO Xiaoshu CHEN Hemei LI Linna ZHEN Feng | 2009 | Chinese Geographical Science2009，19，4： | 5 |
| 2 | Silencing of long non-coding RNA CCHE1 inhibits the ovarian cancer SKOV3 cell invasion and migration and inactivates the p38-MAPK signaling pathway显示文摘Ovarian cancer(OC)is a major cause of cancer-related deaths among gynaecologicalmalignancies.Emerging studies suggest that the long non-coding RNA(lncRNA)may be the potential biomarker for the diagnosis and prognosis of the cancer.The current study was carried out to investigate the role of lncRNA CCHE1 silencing in OC cell invasion and migration.Expression of lncRNA CCHE1 in normal ovarian cell Hose and OC cell lines HO 8910,A2780 and SKOV3 was detected.LncRNA were transfected with siRNA,and then the proliferation of cells was detected by using MTT assay.Cell invasion and migration was measured by using Transwell assay and scratch test,respectively.The protein levels of E-cadherin,N-cadherin,ERK,p38-MAPK and the phosphorylation of ERK and p38-MAPK in cells after siRNA transfection were detected by using Western blot analysis.Consequently,lncRNA CCHE1 expression was highly expressed in OC cell lines,especially in SKOV3 cells.siRNA1,siRNA2 and siRNA3 all decreased.lncRNA CCHE1 expression in SKOV3 cells and siRNA2 showed the best silencing efficacy.Silencing of lncRNA CCHE1 decreased proliferation,invasion and migration,and reduced the protein levels of N-cadherin,ERK,p38-MAPK and the phosphorylation of ERK and p38-MAPK,while reducing the protein level of E-cadherin in SKOV3 cells.Collectively,our study proved that the silencing of lncRNA CCHE1 could inhibit SKOV3 cell invasion and migration via inactivating the p38-MAPK signaling pathway. | HONGWEI CHEN XUAN SONG HEMEI LI | 2020 | BIOCELL2020，44，3： | 0 |
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