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| 1 | Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system显示文摘允许的技术内长的基因的特定的规定为基因功能的学习是珍贵的并且在治疗学有大潜力。我们创造了在 CRISPR 上系统,由核酸酶死者 Cas9 (dCas9 ) 蛋白质组成的二部件的 transcriptional 使活跃之物与互补顺序与 transcriptional 激活域和单个指南 RNA (sgRNAs ) 熔化了到基因倡导者。我们证明在 CRISPR 上能高效地以一种悦耳的方式在人和老鼠房间激活外长的记者基因。另外,我们证明在 vivo 的柔韧的记者基因激活能被把系统部件注入老鼠接合子完成。而且,我们证明在 CRISPR 上能激活内长的 IL1RN, SOX2,和 OCT4 基因。最有效的基因激活被对近似倡导者有约束力的 3-4 sgRNAs 的簇完成,建议他们在基因正式就职的 synergistic 行动。显著地,当指向多重基因的 sgRNAs 同时被介绍进房间时,柔韧的多路的内长的基因激活被完成。染色体宽的表示介绍表明了系统的高特性。 | Albert W Cheng Haoyi Wang Hui Yang Linyu Shi Yarden Katz Thorold W Theunissen Sudharshan Rangarajan Chikdu S Shivalila Daniel B Dadon Rudolf Jaenisch | 2013 | Cell Research2013，23，10： | 65 |
| 2 | Risk stratification in symptomatic intracranial atherosclerotic disease with conventional vascular risk factors and cerebral haemodynamics显示文摘Background and purpose Symptomatic intracranial atherosclerotic stenosis(sICAS)is associated with a considerable risk of recurrent stroke despite contemporarily optimal medical treatment.Severity of luminal stenosis in sICAS and its haemodynamic significance quantified with computational fluid dynamics(CFD)models were associated with the risk of stroke recurrence.We aimed to develop and compare stroke risk prediction nomograms in sICAS,based on vascular risk factors and these metrics.Methods Patients with 50%-99%sICAS confirmed in CT angiography(CTA)were enrolled.Conventional vascular risk factors were collected.Severity of luminal stenosis in sICAS was dichotomised as moderate(50%-69%)and severe(70%-99%).Translesional pressure ratio(PR)and wall shear stress ratio(WSSR)were quantified via CTA-based CFD modelling;the haemodynamic status of sICAS was classified as normal(normal PR&WSSR),intermediate(otherwise)and abnormal(abnormal PR&WSSR).All patients received guideline-recommended medical treatment.We developed and compared performance of nomograms composed of these variables and independent predictors identified in multivariate logistic regression,in predicting the primary outcome,recurrent ischaemic stroke in the same territory(SIT)within 1 year.Results Among 245 sICAS patients,20(8.2%)had SIT.The D2H2A nomogram,incorporating diabetes,dyslipidaemia,haemodynamic status of sICAS,hypertension and age≥50 years,showed good calibration(P for Hosmer-Lemeshow test=0.560)and discrimination(C-statistic 0.73,95%CI 0.60 to 0.85).It also had better performance in risk reclassification and provided larger net benefits in decision curve analysis,compared with nomograms composed of conventional vascular risk factors only,and plus the severity of luminal stenosis in sICAS.Sensitivity analysis in patients with anterior-circulation sICAS showed similar results.Conclusions The D2H2A nomogram,incorporating conventional vascular risk factors and the haemodynamic significance of sICAS as assessed in CFD models,could be a useful tool to stratify sICAS patients for the risk of recurrent stroke under contemporarily optimal medical treatment. | Xuan Tian Hui Fang Linfang Lan Hing Lung Ip Jill Abrigo Haipeng Liu Lina Zheng Florence S Y Fan Sze Ho Ma Bonaventure Ip Bo Song Yuming Xu Jingwei Li Bing Zhang Yun Xu Yannie O Y Soo Vincent Mok Ka Sing Wong Thomas W Leung Xinyi Leng | 2023 | Stroke & Vascular Neurology2023，8，1： | 2 |
| 3 | An Evaluation of the spectral Characteristics of Switching Converters with Chaotic Carrier-Frequency Modulation显示文摘 | Tse K K Ng R W M Chung H Hui S Y R | 2003 | IEEE Trans on Industrial Electronics2003，50，1： | 1 |
| 4 | Carotid Artery Ather- osclerosis: Effect of Intensive Lipid Therapy on the Vasa Vasorum--Evaluation by Using Dynamic Contrast-en- hanced MR Imaging 显示文摘 | Li D Kerwin W S Hui J C | 2011 | Radiology2011，260，1： | 1 |
| 5 | MHC-DQB1 variation and its association with resistance or susceptibility to cystic echinococcosis in Chinese Merino Sheep 显示文摘 | Hui W Shen H Jiang S | 2012 | Asian- Australasian Journal of Animal Sciences2012，25，12： | 1 |
| 6 | A critical review of recent progress in mid-range wireless power transfer 显示文摘 | HUI S Y R ZHONG W X LEE C K | 2014 | IEEE Transactions on Power Electronics2014，60，9： | 1 |
| 7 | Potential use of a combined ozone and zeolite system for gaseous toluene elimination显示文摘 | CHAO C Y H KWONG C W HUI K S | 2007 | Journal ofHazardous and materials2007，143，12： | 1 |
| 8 | Inhibition of pyocyanin-potentiated IL-8 release by steroids in bronchial epithelial cells显示文摘 | Pan N Y Hui W S Tipoe G L | 2006 | Respir Med2006，100，： | 1 |
| 9 | Wireless Power Domino-Resonator Systems with Noncoaxial Axes and Circular Structures显示文摘 | Zhong W X Chi Kwan L Hui S Y | 2012 | IEEE Transactions on Power Electronics2012，27，11： | 1 |
| 10 | Transdermal insulin delivery using lipid enhanced electroporation显示文摘 | SEN A DALY M E HUI S W | 2002 | Biochim Biophys Acta2002，1564，： | 1 |
| 11 | An isolated ZVS/ZCS flyback converter using the leakage inductance of the coupled inductor显示文摘 | Chung H Hui S Y R Wang W H | 1998 | Industrial Electronics IEEE Transactions1998，45，4： | 1 |
| 12 | The crystal structure of theβ′phase in Al-Mg-Si alloys显示文摘 | VISSERS R HUIS M A JANSEN J ZANDBERGEN H W MARIOARA C D ANDERSEN S J | 2007 | Acta Materialia2007，55，11： | 1 |
| 13 | The role of coordinates in the computation of discontinuities in one-dimensional flow 显示文摘 | Hui W H Koudriakov S | 2000 | CFD J2000，8，： | 1 |
| 14 | Elimination of electrolytic capacitor in an AC/DC light-emitting diode (LED) driver with high input power factor and constant output Current 显示文摘 | Chen W and Hui S Y R | 2012 | IEEE Trans on Power Electron2012，27，3： | 1 |
| 15 | A unified coordinate system for solving the three-dimensional Euler equations 显示文摘 | Hui W H Koudriakov S | 2001 | J Comput Phys2001，172，： | 1 |
| 16 | Inhibition of pyocyanin-potentiated IL-8 release by steroids in bronchial epithelial cells显示文摘 | Pan N Y Hui W S Tipoe G L | 2006 | Respir Med2006，100，： | 1 |
| 17 | Propofol effective concentration 50 and its relationship to bispectral index显示文摘 | Hui T W Milne S E | 2002 | Anaesthesia2002，57，3： | 1 |
| 18 | Video-assisted thoracic surgery lohectomy for lung cancer is associated with less immunochemokine disturbances than thoracotomy显示文摘 | Ng C S Wan S Hui C W | 2007 | Eur J Cardiothorac Surg2007，31，： | 1 |
| 19 | Antiviral agents and corticosteroids in the treatment of severe acute respiratory syndrome (SARS)显示文摘 | YU W C HUI D S C CHAN-YEUNG M | 2004 | Thorax2004，59，： | 1 |
| 20 | Video-assisted thoracic surgery for early stage lung cancer-can short-term immunological advanta- ges improve long-term survival? 显示文摘 | Ng C S Wan S Hui C W | 2006 | Ann Thorac Cardiovasc Surg2006，12，5： | 1 |
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