|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Forms of nitrogen uptake,translocation,and transfer via arbuscular mycorrhizal fungi:A review显示文摘Arbuscular mycorrhizal(AM) fungi are obligate symbionts that colonize the roots of more than 80% of land plants.Experiments on the relationship between the host plant and AM in soil or in sterile root-organ culture have provided clear evidence that the extraradical mycelia of AM fungi uptake various forms of nitrogen(N) and transport the assimilated N to the roots of the host plant.However,the uptake mechanisms of various forms of N and its translocation and transfer from the fungus to the host are virtually unknown.Therefore,there is a dearth of integrated models describing the movement of N through the AM fungal hyphae.Recent studies examined Ri T-DNA-transformed carrot roots colonized with AM fungi in 15 N tracer experiments.In these experiments,the activities of key enzymes were determined,and expressions of genes related to N assimilation and translocation pathways were quantified.This review summarizes and discusses the results of recent research on the forms of N uptake,transport,degradation,and transfer to the roots of the host plant and the underlying mechanisms,as well as research on the forms of N and carbon used by germinating spores and their effects on amino acid metabolism.Finally,a pathway model summarizing the entire mechanism of N metabolism in AM fungi is outlined. | JIN HaiRu LIU Jie LIU Jing HUANG XiaoWei | 2012 | Science China(Life Sciences)2012，55，6： | 21 |
| 2 | Arginine bi-directional translocation and breakdown into ornithine along the arbuscular mycorrhizal mycelium显示文摘Bi-directional translocation and degradation of Arginine (Arg) along the arbuscular mycorrhizal (AM) fungal mycelium were testified through 15N and/or 13C isotopic labeling. In vitro mycorrhizas of Glomus intraradices and Ri T-DNA-transformed carrot roots were grown in dual compartment Petri dishes. [15N- and/or13C]Arg was supplied to either the fungal compartment or the mycorrhizal compartment or separate dishes containing the uncolonized roots. The levels and labeling of free amino acids (AAs) in the mycorrhizal roots and in the extraradical mycelia(ERM) were measured by gas chromatogra- phy/mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC). The ERM of AM fungi exposed in either NH4+ or urea as sole external nitrogen source had much higher 15N enrichment of Arg, compared with those in nitrate or exogenous Arg; however, glycerol supplied as an external car- bon source to the ERM had no significant effect on the level of Arg in the ERM. Meanwhile, Arg bio- synthesized in the ERM could be translocated intact to the mycorrhizal roots and thereby the level of Arg in the mycorrhizal roots increased to about 20% after culture of ERM in 4 mmol/L NH4+ for 6 weeks. Also Arg was found to be bi-directionally transported along the AM fungal mycelium through [U-13C]Arg labeling either in the mycorrhizal compartment or in the fungal compartment. Once Arg was translo- cated to the potential N-limited sites, it would be further degraded into ornithine (Orn) and urea since either [U-13C] or [U-15N/U-13C]Orn was apparently shown up in the mycorrhizal root tissues when [U-13C] or [U-15N/U-13C]Arg was labeled in the fungal compartment, respectively. Evidently Orn formation indi- cated the ongoing activities of Arg translocation and degradation through the urea cycle in AM fungal mycelium. | JIN HaiRu College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China | 2009 | Science China(Life Sciences)2009，52，4： | 16 |
| 3 | Nitrogen transfer in the arbuscular myeorrhizal symbiosis 显示文摘 | Govindarajulu M Pfeffer P E Jin Hairu | 2005 | Nature2005，435，： | 1 |
| 4 | By-product formation by a novel glycerol-producing yeast, Candida glyccrinogencs, with different O2 supplies显示文摘 | Hairu Jin Huiying Fang Jian Zhuge | 2003 | Biotechnology Letters2003，，25： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员