|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Molecular Cloning and Expression Analysis of the ζ-Carotene Desaturase Gene in Chinese kale(Brassica oleracea var.alboglabra Bailey)显示文摘ζ-Carotene desaturase(ZDS)is an important enzyme in carotenoid biosynthesis.Here,the Brassica oleracea var.alboglabra ZDS(Boa ZDS)gene was cloned from Chinese kale via reverse transcription-polymerase chain reaction(RT-PCR)and deposited in Gen Bank(accession number KY662297).The Boa ZDS gene contains an open reading frame of 1 686 bp that encodes a 561-amino acid protein.Sequence analysis indicates that the ZDS protein is apparently conserved during plant evolution and is most closely related to B.oleracea var.capitata and B.rapa.The promoter sequence of the Boa ZDS gene was predicted to harbor several cis-acting elements that are related to light and phytohormone responses.Semiquantitative RT-PCR analysis showed that Boa ZDS expression varied among different developmental stages and organs.Relative ZDS expression remained stable during germination and seedling stages and rapidly increased at the mature leaf stage.The leaves showed the highest ZDS expression levels compared to the other organs.ZDS expression decreased in all flower tissues during blooming.The fused protein of Boa ZDS was obtained by prokaryotic expression.Heterologous expression of Boa ZDS in Escherichia coli confirmed that Boa ZDS encodes a functionalζ-carotene desaturase that increases β-carotene accumulation in E.coli cells harboring a β-carotene-producing plasmid.The findings of the present study provide a molecular basis for the elucidation of ZDS gene function in Chinese kale. | SUN Bo ZHANG Fen XUE Shengling CHANG Jiaqi ZHENG Aihong JIANG Min MIAO Huiying WANG Qiaomei TANG Haoru | 2018 | Horticultural Plant Journal2018，4，3： | 10 |
| 2 | Comparative transcriptome analysis uncovers the regulatory functions of long noncoding RNAs in fruit development and color changes of Fragaria pentaphylla显示文摘To investigate the molecular mechanism underlying fruit development and color change,comparative transcriptome analysis was employed to generate transcriptome profiles of two typical wild varieties of Fragaria pentaphylla at three fruit developmental stages(green fruit stage,turning stage,and ripe fruit stage).We identified 25,699 long noncoding RNAs(lncRNAs)derived from 25,107 loci in the F.pentaphylla fruit transcriptome,which showed distinct stage-and genotype-specific expression patterns.Time course analysis detected a large number of differentially expressed protein-coding genes and lncRNAs associated with fruit development and ripening in both of the F.pentaphylla varieties.The target genes downregulated in the late stages were enriched in terms of photosynthesis and cell wall organization or biogenesis,suggesting that lncRNAs may act as negative regulators to suppress photosynthesis and cell wall organization or biogenesis during fruit development and ripening of F.pentaphylla.Pairwise comparisons of two varieties at three developmental stages identified 365 differentially expressed lncRNAs in total.Functional annotation of target genes suggested that lncRNAs in F.pentaphylla may play roles in fruit color formation by regulating the expression of structural genes or regulatory factors.Construction of the regulatory network further revealed that the low expression of Fra a and CHS may be the main cause of colorless fruit in F.pentaphylla. | Lijun Bai Qing Chen Leiyu Jiang Yuanxiu Lin Yuntian Ye Peng Liu Xiaorong Wang Haoru Tang | 2019 | Horticulture Research2019，6，1： | 5 |
| 3 | Effect of Heat Treatment Combined with Shoot Tip Culture on the Virus-Free of Arena Strawberry显示文摘 | Huifeng Jia Haoru Tang | 2012 | Journal of Life Sciences2012，6，2： | 1 |
| 4 | Plant regeneration from leaves of sweet and sour cherry cultivars显示文摘 | Tang Haoru Ren Zhenglong Reustle G | 2002 | Scientia Horticulturae2002，93，： | 1 |
| 5 | Comparative metabolomics provides novel insights into the basis of petiole color differences in celery(Apium graveolens L.)显示文摘Plant metabolites are important for plant development and human health.Plants of celery(Apium graveolens L.)with different-colored petioles have been formed in the course of long-term evolution.However,the composition,content distribution,and mechanisms of accumulation of metabolites in different-colored petioles remain elusive.Using ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS),1159 metabolites,including 100 lipids,72 organic acids and derivatives,83 phenylpropanoids and polyketides,and several alkaloids and terpenoids,were quantified in four celery cultivars,each with a different petiole color.There were significant differences in the types and contents of metabolites in celery with different-colored petioles,with the most striking difference between green celery and purple celery,followed by white celery and green celery.Annotated analysis of metabolic pathways showed that the metabolites of the different-colored petioles were significantly enriched in biosynthetic pathways such as anthocyanin,flavonoid,and chlorophyll pathways,suggesting that these metabolic pathways may play a key role in determining petiole color in celery.The content of chlorophyll in green celery was significantly higher than that in other celery cultivars,yellow celery was rich in carotenoids,and the content of anthocyanin in purple celery was significantly higher than that in the other celery cultivars.The color of the celery petioles was significantly correlated with the content of related metabolites.Among the four celery cultivars,the metabolites of the anthocyanin biosynthesis pathway were enriched in purple celery.The results of quantitative real-time polymerase chain reaction(q RT-PCR)suggested that the differential expression of the chalcone synthase(CHS)gene in the anthocyanin biosynthesis pathway might affect the biosynthesis of anthocyanin in celery.In addition,HPLC analysis revealed that cyanidin is the main pigment in purple celery.This study explored the differences in the types and contents of metabolites in celery cultivars with different-colored petioles and identified key substances for color formation.The results provide a theoretical basis and technical support for genetic improvement of celery petiole color. | Mengyao LI Jie LI Haohan TAN Ya LUO Yong ZHANG Qing CHEN Yan WANG Yuanxiu LIN Yunting ZHANG Xiaorong WANG Haoru TANG | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，4： | 0 |
| 6 | Effects Analysis of Mg^2+, dNTPs and Taq DNA Polymerase on SSR-PCR System of Pear显示文摘 | Min Lu Xiaoyang Chen Ling Lin Ya Luo Yong Zhang Zejing Liu Liyi Li Haoru Tang | 2011 | Journal of Life Sciences2011，5，6： | 0 |
| 7 | Expression Analysis of the ANS Gene in Fragaria x ananassa cv, Toyonaka显示文摘 | Xiaonan Zhang Qing Chen Haowei Yu Shuli Zhou Haoru Tang | 2012 | Journal of Life Sciences2012，6，11： | 0 |
| 8 | Application Status,Problems and Countermeasures of Dairy Herd Improvement( DHI) in Dairy Buffalo Industry显示文摘The development of buffalo milk industry in China encounters the problems of small high yield populations and insufficient excellent provenance. Therefore,it is necessary to carry out dairy herd improvement( DHI) to increase dairy buffalo herd productivity. This paper reviewed the situation and problems of DHI in dairy buffalo,and the corresponding opinions and suggestions were put forward. | Lin Bo Li Ling Tang Yan Nong Haoru Zeng Qingkun | 2016 | Animal Husbandry and Feed Science2016，8，1： | 0 |
| 9 | Experiment and analysis of the formation,expansion and dissipation of gasbag in fine sediments based on pore water pressure survey显示文摘Deep-seated gas in seabed sediments migrates upwards from effect of external factors,which easily accumulates to form gasbags at interface of shallow coarse-fine sediments.Real-time monitoring of this process is important to predict disaster.However,there is still a lack of effective monitoring methods,so we attempt to apply multi-points pore water pressure monitoring technology when simulating forming and dissipation of gasbags in sediments through laboratory experiment.This study focuses on discussion of sensitivity of pore water pressure monitoring data,as well as typical changing characteristics and mechanisms of excess pore water pressure corresponding to crack generation,gasbag formation and gas release.It was found that the value of excess pore water pressure in sediments is negatively correlated with vertical distance between sensors and gas source,and the evolution of gasbag forming and dissipation has a good corresponding relationship with the change of excess pore water pressure.Gasbag formation process is divided into three stages:transverse crack development,longitudinal cavity expansion,and oblique crack development.Formation of gasbag begins with the transverse crack at the interface of coarse-fine sediments while excess pore water pressure attenuates rapidly and then drops,pressure remains almost unchanged when cavity expanses longitudinally,oblique crack appeared in final stage of gasbag evolution while excess pore water pressure accumulated and dissipated again.The variation curve of excess pore water pressure in gas release stage has saw-tooth fluctuation characteristics,and the value and time of pressure accumulation are also fluctuating,indicating the uncertainty and non-uniqueness of gas migration channels in sediments. | Shiyun Lei Xiujun Guo Haoru Tang | 2022 | Acta Oceanologica Sinica2022，41，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员