| 1 | Determination of conifer age biomarker DAL1 interactome using Y2H-seq显示文摘Age is a sophisticated physiological signal that ensures the sequence of different developmental stages in organisms.The regulation of ageing pathways appears to differ between gymnosperms and angiosperms.We previously identified DAL1 as a conserved conifer age biomarker that plays a crucial role in the transition from vegetative to reproductive life-history phases in pines.Therefore,elucidating the specific interaction events related to DAL1 is key to understanding how age drives conifer development.Large-scale yeast two-hybrid(Y2H)analysis followed by next-generation high-throughput sequencing(Y2H-seq)allowed us to identify 135 PtDAL1 interacting proteins in Pinus tabuliformis.Our study found that PtDAL1 interacting proteins showed an ageing-related module,with sophisticated interacting networks composed of transcription factors(TFs),transcriptional regulators(TRs),and kinases.These interacting proteins are produced in response to a variety of phytohormones and environmental signals,and are likely involved in wood formation,needle development,oleoresin terpenoids biosynthesis,and reproductive development.In this study,we propose a novel regulation model of conifer ageing pathways whereby PtDAL1 coordinates different environmental stimuli and interacts with corresponding proteins to regulate appropriate development. | Xi Chen Qianya Zhu Yumeng Nie Fangxu Han Yue Li Harry XWu Shihui Niu | 2021 | Forestry Research2021，1，1： | 0 |
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