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	题名	作者	年代	出处	被引量
1	hsa-mir-183 is frequently methylated and related to poor survival in human hepatocellular carcinoma显示文摘AIM To screen clinically relevant micro RNAs (mi RNAs) silenced by DNA methylation in human hepatocellular carcinoma(HCC).METHODS Knockdown of DNA methyltransferases (DNMTs) using si RNAs and mi RNA profiling in HCC cell lines were performed to identify DNA hypermethylation-mediated mi RNA downregulation. Confirmation using individual quantitative real-time PCR (qR T-PCR) assays was thenperformed followed by DNA methylation quantification at the promoter of the mi RNA genes. Quantification of DNA methylation and mi RNA expression was then performed in primary HCC tumor samples and related with clinicopathological variables.RESULTS mi RNA profiling after DNMT knockdown in HCC cell lines revealed upregulation of mi R-23, mi R-25 and mi R-183. After q RT-PCR confirmation and Cp G island methylation quantification of these miR NAs in cell lines, further analysis in primary HCC specimens showed that hsa-mi R-183 is hypermethylated in 30% of HCC (n = 40). Expression of mature miR-183 showed an inverse correlation with DNA methylation levels. In HCC cells, DNMT knockdown and 5-aza-2'-deoxycytidine treatment reduced methylation and stimulated expression of mi R-183. In HCC patients, hypermethylation at hsami R-183 promoter significantly correlates with poor survival (log-rank test P = 0.03). DNA methylation analysis in healthy liver, benign liver tumors (hepatocellular adenoma and focal nodular hyperplasia) and their corresponding adjacent tissues showed absence of hypermethylation supporting the notion that aberrant methylation at hsa-miR-183 is specific for the malignant transformation of hepatocytes.CONCLUSION Our data indicate that hypermethylation of hsa-miR-183 is a frequent event in HCC and potentially useful as a novel surrogate diagnostic and prognostic marker.	Sumadi Lukman Anwar Till Krech Britta Hasemeier Elisa Schipper Nora Schweitzer Arndt Vogel Hans Kreipe Reena Buurman Britta Skawran Ulrich Lehmann	2017	World Journal of Gastroenterology2017，23，9：	3
2	Reliable micro RNA profiling in routinely processed formalin-fixed paraffin-embedded breast cancer specimens using fluorescence labelled bead technology显示文摘	Hasemeier B Christgen M Kreipe H	2008	BMC Biotechnol2008，8，：	1
3	Epigenetic inactivation of micro RNA gene hsa-miR-9-1 in human breast cancer显示文摘	LEHMANN U HASEMEIER B CHRISTGEN M	2008	J Pathol2008，214，：	1
4	Loss of imprinting and allelic switching at the DLK1-MEG3 locus in human hepatocellular carcinoma 显示文摘	Anwar SL Krech T Hasemeier B	2012	PLoS One2012，7，49：	1
5	Epigenetic inac- tivation of microRNA gene has-mir-9-1 inhuman breast can- cer显示文摘	Lehmann U Hasemeier B Chfistgen M	2008	J Pathol2008，214，1：	1
6	Loss of imprin-ting and allelic switching at the DLK1-MEG3 locus in human hepatocellular carcinoma显示文摘	Anwar SL Krech T Hasemeier B	2012	PLoS 0ne2012，7，49：	1
7	Loss of imprinting and al- lelic switching at the DLK1-MEG3 locus in human hepatocellular carcinoma显示文摘	Anwar SL Krech T Hasemeier B	2012	PLoS One2012，7，49：	1
8	Loss of imprinting and allelic switching at the DLK1-MEG3 locus in human hepatocellular carcinoma显示文摘	Anwar SL Krech T Hasemeier B	2012	PLoS One2012，7，49：	1
9	Epigenetic inactivation of microRNA gene hsa-mir-9-1 in human breast cancer显示文摘	Lehmann U Hasemeier B Christgen M	2008	J Pathol2008，214，1：	1
10	Promoter hypermethylation of the death-associated protein kinase gene in breast cancer is associated with the invasive lobular subtype显示文摘	Celikkaya G Hasemeier B	2002	Cancer Res2002，62，22：	1
11	Reliable microRNA profiling in routinely processed formalinfixed paraffin-embedded breast cancer specimens using fluorescence labelled bead technology显示文摘	Hasemeier B Christgen M Kreipe H	2008	BMC Biotechnol2008，8，11：	1
12	Loss of imprinting and allelic switching at the DLK1-MEG3 locus in human hepatocellular carcinoma显示文摘	Anwar SL Krech T Hasemeier B	2012	PLoS One2012，7，49：	1
13	Epigenetic inactivation of microRNA gene hsa-mir-9-1 in human breast cancer 显示文摘	Lehmann U Hasemeier B Christgen M	2008	J Pathol2008，214，：	1
14	Epigenetic inactiva-tion of microRNA gene hsa - mir -9-1 in human breast cancer显示文摘	Lehmann U Hasemeier B Christgen M	2008	J Pathol2008，214，1：	1
15	Epigenetic inac- tivation of mieroRNA gene has-mir-9-1 inhuman breast can- cer显示文摘	Lehmann U Hasemeier B Christgen M	2008	J Pathol2008，214，1：	1
16	Global increase in DNA methylation in patients with myelodysplastic syndrome显示文摘	Romermann D Hasemeier B Metzig K	2008	Leukemia2008，22，10：	1
17	SRSF2 muta- tion is present in the hypercellular and prefibrotic stage of primary myelofibrosis 显示文摘	Lehmann U Barrels S Hasemeier B	2013	Blood2013，121，19：	1
18	Epigenetic inactivation of microRNA gene has-mir-9-1 in human breast cancer 显示文摘	LEHMANN U HASEMEIER B CHRISTGEN M	2008	J Pathol2008，214，1：	1
19	Epigenetic Inactivation of MicroRNA Gene Hsa-mir-9-1 in Human Breast Cancer显示文摘	LEHMANN U HASEMEIER B CHRISTGEN M	2008	Journal of Pathology2008，214，1：	1
20	Epigenetic inactivation of microRNA gene in human breast cancer显示文摘	Lehmann U Hasemeier B Christgen M	2008	J Pathol2008，214，1：	1

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