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| 1 | DNA methylation,microRNAs,and their crosstalk as potential biomarkers in hepatocellular carcinoma显示文摘Epigenetic alterations have been identified as a major characteristic in human cancers.Advances in the field of epigenetics have contributed significantly in refining our knowledge of molecular mechanisms underlying malignant transformation.DNA methylation and microRNA expression are epigenetic mechanisms that are widely altered in human cancers including hepatocellular carcinoma(HCC),the third leading cause of cancer related mortality worldwide.Both DNA methylation and microRNA expression patterns are regulated in developmental stage specific-,cell type specific-and tissue-specific manner.The aberrations are inferred in the maintenance of cancer stem cells and in clonal cell evolution during carcinogenesis.The availability of genome-wide technologies for DNA methylation and microRNA profiling has revolutionized the field of epigenetics and led to the discovery of a number of epigenetically silenced microRNAs in cancerous cells and primary tissues.Dysregulation of these microRNAs affects several key signalling pathways in hepatocarcinogenesis suggesting that modulation of DNA methylation and/or microRNA expression can serve as new therapeutic targets for HCC.Accumulative evidence shows that aberrant DNA methylation of certain microRNA genes is an event specifically found in HCC which correlates with unfavorable outcomes.Therefore,it can potentially serve as a biomarker for detection as well as for prognosis,monitoring and predicting therapeutic responses in HCC. | Sumadi Lukman Anwar Ulrich Lehmann | 2014 | World Journal of Gastroenterology2014，20，24： | 11 |
| 2 | hsa-mir-183 is frequently methylated and related to poor survival in human hepatocellular carcinoma显示文摘AIM To screen clinically relevant micro RNAs (mi RNAs) silenced by DNA methylation in human hepatocellular carcinoma(HCC).METHODS Knockdown of DNA methyltransferases (DNMTs) using si RNAs and mi RNA profiling in HCC cell lines were performed to identify DNA hypermethylation-mediated mi RNA downregulation. Confirmation using individual quantitative real-time PCR (qR T-PCR) assays was thenperformed followed by DNA methylation quantification at the promoter of the mi RNA genes. Quantification of DNA methylation and mi RNA expression was then performed in primary HCC tumor samples and related with clinicopathological variables.RESULTS mi RNA profiling after DNMT knockdown in HCC cell lines revealed upregulation of mi R-23, mi R-25 and mi R-183. After q RT-PCR confirmation and Cp G island methylation quantification of these miR NAs in cell lines, further analysis in primary HCC specimens showed that hsa-mi R-183 is hypermethylated in 30% of HCC (n = 40). Expression of mature miR-183 showed an inverse correlation with DNA methylation levels. In HCC cells, DNMT knockdown and 5-aza-2'-deoxycytidine treatment reduced methylation and stimulated expression of mi R-183. In HCC patients, hypermethylation at hsami R-183 promoter significantly correlates with poor survival (log-rank test P = 0.03). DNA methylation analysis in healthy liver, benign liver tumors (hepatocellular adenoma and focal nodular hyperplasia) and their corresponding adjacent tissues showed absence of hypermethylation supporting the notion that aberrant methylation at hsa-miR-183 is specific for the malignant transformation of hepatocytes.CONCLUSION Our data indicate that hypermethylation of hsa-miR-183 is a frequent event in HCC and potentially useful as a novel surrogate diagnostic and prognostic marker. | Sumadi Lukman Anwar Till Krech Britta Hasemeier Elisa Schipper Nora Schweitzer Arndt Vogel Hans Kreipe Reena Buurman Britta Skawran Ulrich Lehmann | 2017 | World Journal of Gastroenterology2017，23，9： | 3 |
| 3 | Common and epithelioid variants of hepatic angiomyolipoma exhibit clonal growth and share a distinctive immunophenotype显示文摘 | Peer Flemming Ulrich Lehmann Thomas Becker Jürgen Klempnauer Hans Kreipe | 2000 | Hepatology2000，，： | 2 |
| 4 | Limited value of KAI1/CD82 protein expression as a prognosticmarker in human gastric cancer显示文摘 | Maximilian Knoener Till Krech Florian Puls Ulrich Lehmann Hans Kreipe Matthias Christgen | 2012 | Disease Markers2012，，6： | 1 |
| 5 | Intratumoral Onyx embolisation in the management of juvenile nasopharyn gem angiofibroma显示文摘 | LEHMANN M ULRICH S REINEKE U | 2010 | Hno2010，58，8： | 1 |
| 6 | ABCB1/MDR1 contributes to the anticancer drug-resistant phenotype of IPH-926 human lobular breast cancer cells显示文摘 | Till Krech Elisa Scheuerer Robert Geffers Hans Kreipe Ulrich Lehmann Matthias Christgen | 2011 | Cancer Letters2011，，2： | 1 |
| 7 | Plexiform Lesions in Pulmonary Arterial Hypertension显示文摘 | Danny Jonigk Heiko Golpon Clemens L. Bockmeyer Lavinia Maegel Marius M. Hoeper Jens Gottlieb Nils Nickel Kais Hussein Ulrich Maus Ulrich Lehmann Sabina Janciauskiene Tobias Welte Axel Haverich Johanna Rische Hans Kreipe Florian Laenger | 2011 | The American Journal of Pathology2011，，1： | 1 |
| 8 | GNB3 gene 825 TT variant predicts hard coronary events in the population-based Heinz Nixdorf Recall study显示文摘 | Ulrich H. Frey Susanne Moebus Stefan M?hlenkamp Hagen K?lsch Marcus Bauer Nils Lehmann Markus N?then Thomas W. Mühleisen Andreas Stang Raimund Erbel Karl-Heinz J?ckel Jürgen Peters Winfried Siffert | 2014 | Atherosclerosis2014，，2： | 1 |
| 9 | Concordant hypermethylation of intergenic microRNA genes in human hepatocellular carcinoma as new diagnostic and prognostic marker显示文摘 | Sumadi Lukman Anwar Cord Albat Till Krech Britta Hasemeier Elisa Schipper Nora Schweitzer Arndt Vogel Hans Kreipe Ulrich Lehmann | 2013 | Int J Cancer2013，，3： | 1 |
| 10 | Telomerase Deletion Limits Progression of p53-Mutant Hepatocellular Carcinoma With Short Telomeres in Chronic Liver Disease显示文摘 | André Lechel Henne Holstege Yvonne Begus Andrea Schienke Kenji Kamino Ulrich Lehmann Stefan Kubicka Peter Schirmacher Jos Jonkers K. Lenhard Rudolph | 2007 | Gastroenterology2007，，4： | 1 |
| 11 | Intravenous free and dipeptide-bound glutamine maintains intestinal microcirculation in experimental endotoxemia显示文摘 | Christian Lehmann Dragan Pavlovic Juan Zhou Ulrich Wuttke Daniela Saeger Alexander Spassov Orlando Hung Vladimir Cerny Tobias Witter Sara Whynot Ulrich Suchner Birgit Alteheld Peter Stehle Matthias Gründling | 2012 | Nutrition2012，，5： | 1 |
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