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| 1 | Effects of insulin and pathway inhibitors on the PI3K-Akt- mTOR phosphorylation profile in acute myeloid leukemia cells显示文摘The phosphatidylinositol 3-kinase(PI3K)-Akt-mechanistic target of rapamycin(mTOR)pathway is constitutively activated in human acute myeloid leukemia(AML)cells and is regarded as a possible therapeutic target.Insulin is an agonist of this pathway and a growth factor for AML cells.We characterized the effect of insulin on the phosphorylation of 10 mediators in the main track of the PI3K-Akt-mTOR pathway in AML cells from 76 consecutive patients.The overall results showed that insulin significantly increased the phosphorylation of all investigated mediators.However,insulin effects on the pathway activation profile varied among patients,and increased phosphorylation in all mediators was observed only in a minority of patients;in other patients,insulin had divergent effects.Global gene expression profiling and proteomic/phosphoproteomic comparisons suggested that AML cells from these two patient subsets differed with regard to AML cell differentiation,transcriptional regulation,RNA metabolism,and cellular metabolism.Strong insulin-induced phosphorylation was associated with weakened antiproliferative effects of metabolic inhibitors.PI3K,Akt,and mTOR inhibitors also caused divergent effects on the overall pathway phosphorylation profile in the presence of insulin,although PI3K and Akt inhibition caused a general reduction in Akt pT308 and 4EBP1 pT36/pT45 phosphorylation.For Akt inhibition,the phosphorylation of upstream mediators was generally increased or unaltered.In contrast,mTOR inhibition reduced mTOR pS2448 and S6 pS244 phosphorylation but increased Akt pT308 phosphorylation.In conclusion,the effects of both insulin and PI3K-Akt-mTOR inhibitors differ between AML patient subsets,and differences in insulin responsiveness are associated with differential susceptibility to metabolic targeting. | Ina Nepstad Kimberley Joanne Hatfield Ida Sofie Grønningsæter Elise Aasebø Maria Hernandez-Valladares Karen Marie Hagen Kristin Paulsen Rye Frode SBerven Frode Selheim Håkon Reikvam Øystein Bruserud | 2019 | Signal Transduction and Targeted Therapy2019，4，1： | 4 |
| 2 | 新致病基因在颅缝早闭Crouzon综合征中的初步机制研究显示文摘目的研究Rbp4(Retinol binding protein 4)、Gpc3(Glypican family of growth factor binding protein 3)、C1qtnf3(Collagenous repeat-containing sequence of 26 KDa protein)等新致病基因在颅缝早闭症中的发病机制,为疾病非手术治疗奠定理论基础。方法以Crouzon综合征Fgfr2cC342Y/+小鼠为实验模型,应用MicroCT和组织学染色,研究小鼠颅缝闭合模式;以Fgfr2cC342Y/+模型,RT-qPCR研究Rbp4、Gpc3、C1qtnf3等新致病基因的表达差异,初步探讨其在颅缝闭合过程中的调控作用;以体外培养的Fgfr2cC342Y/+小鼠颅缝细胞为模型,研究基因突变动物细胞增殖与代谢改变。结果获得Fgfr2cC342Y/+小鼠后额缝、冠状缝、人字缝、矢状缝等颅缝闭合模式,随颅骨发育、颅缝闭合,OC(Osteocalcin)、ALP(Alkalinephosphatase)表达增加,目的基因Rbp4、Gpc3、C1qtnf3表达下降,Msx2(Muscle segment homeobox gene 2)表达增加,杂合子与野生型小鼠之间均存在显著统计学差异,与前期人颅缝组织Microarray研究结果一致。Gpc1(Glypican family ofgrowth factor binding protein 1)、FliI(Flightless I)在颅缝闭合中的表达较恒定,野生型与杂合子之间未见明显统计学差异。体外培养Fgfr2cC342Y/+小鼠颅缝细胞,CellTiter96 MTS和Quant-iT Picogreen dsDNA细胞增殖与代谢分析结果显示,Fgfr2功能获得性突变可促进冠状缝细胞的增殖,从而导致成骨增加,颅缝早闭。结论 Fgfr2cC342Y/+模型新致病基因表达趋势与前期人颅缝组织Microarray研究结果一致,Rbp4、Gpc3、C1qtnf3可能在颅缝早闭中具重要调控作用。 | 杨娴娴 Jodie T Hatfield Susan J Hinze 穆雄铮 Peter J Anderson Barry C Powell | 2012 | 组织工程与重建外科杂志2012，8，5： | 3 |
| 3 | 文化差异与性的自我表露显示文摘性的自我表露(sexual self-disclosure)是自我表露中最亲密的形式。它是一个亲密的二人关系中一方把他(她)的性事表露给伴侣的程度。出乎意料的是,和对于大量关于其他方面的自我表露相比,学界对这方面的研究很少。在这些为数不多的研究中,一些研究发现:性的自我表露和人们的性满意度是相关的。因此研究性的自我表露或许有助于了解或增益人们的性生活。过去的研究从未调查过文化是否影响人们对性的自我表露。在不同的文化中,人们性的自我表露的程度和内容都应该会有所差异。现有的关于人们自我表露中的文化差异的理论研究对未来研究性的自我表露受文化差异的影响提供了方向。 | 唐女 Elaine HATFIELD | 2014 | 心理科学进展2014，22，1： | 2 |
| 4 | Selenocysteine tRNAs as Central Components of Selenoprotein Biosynthesis in Eukaryotes显示文摘Selenocysteine (Sec) tRNAs serve as carrier molecules for the biosynthesis of Sec from serine and to donate Sec to protein in response to specific UGA codons. In this study, we describe the current status of Sec tRNAs in higher animals and further we exarnine: (i) the Sec tRNA population in Drosophila; (ii) transcription of the Sec tRNA in vivo (in Xenopus oocytes) and in vitro (in Xenopus oocyte extracts); (iii) the effect of selenium on the Sec tRNA population in various rat tissues following replenishment of extremely selenium deficient rats with this element; and (iv) the biosynthesis of the modified bases on Sec tRNA in Xenopus | SANG ICK PARK JIN Mo PARK HAROLD S. CHITTUM EUN SUNG YANG BRADLEY A. CARLSON BYEONG JAE LEE AND DOLPH L. HATFIELD(Laboratory of Experimental Chrcinogenesis, National Cancer Insti tute, National Institutes of Health, Bethesda, MD 20892USA Laboratory o | 1997 | Biomedical and Environmental Sciences1997，10，2： | 2 |
| 5 | Stem cell division is regulated by the microRNA pathway显示文摘 | HATFIELD S D SHCHERBATA H R FISCHER K A | 2005 | Nature2005，435，7044： | 1 |
| 6 | Estimating absorbed photosynthetically active radiation and leaf area index from spectral reflectance in wheat显示文摘 | ASRAR G M FUCHS E T KANEMASU J HATFIELD L | 1984 | Agronomy Journal1984，76，： | 1 |
| 7 | Discerning the forest from the trees:an essay on scaling canopy stomatal conductance显示文摘 | LUXMOORE R J HATFIELD J L | 1991 | Agric For Meterol1991，54，： | 1 |
| 8 | Ferulate cross-linking in cell walls isolated from maize cell suspensions 显示文摘 | Grabber J H Hatfield R D Ralph J | 1995 | Phytochemistry1995，40，： | 1 |
| 9 | A meta- analysis on the anxiety - reducing effects of acute and chronic exercise 显示文摘 | Petruzzello S J Landers D M Hatfield B D | 1991 | Sports Medicine1991，11，3： | 1 |
| 10 | Using the Cancer Rehabilitation Questionnaire in patients with colo- rectal cancer显示文摘 | Nicholas P K Leuner J D Hatfield J M | 2006 | Rehabil Nurs2006，31，3： | 1 |
| 11 | Intercepted photosynthetically active radiation in wheat canopies estimated by spectral reflectance 显示文摘 | Hatfield J L Asrar G Kanemasu E T | 1984 | Remote Sensing of Environment1984，14，123： | 1 |
| 12 | Estimation of Daily Evapotranspiration from One Time-of-day Measurements显示文摘 | Hatfield J L Reginato R J | 1983 | Agric Water Manage1983，7，： | 1 |
| 13 | Web-based three-dimensional virtual body Structures:W3D-VBS显示文摘 | Temkin B Acosta E Hatfield P | 2002 | J Am Med Inform Assoc2002，9，5： | 1 |
| 14 | Phytochrome A overex- pression in transgenic tobacco: Correlation of dwarf phenotype with high concentrations of phytochrome in vascular tissue and attenuated gibberellin levels 显示文摘 | Jordan E T Hatfield P M Hondred D | 1995 | Plant Physiol1995，107，： | 1 |
| 15 | Principles and guidelines for the design of eye/voice interaction dialogs显示文摘 | Franz Hatfield Eric A Jenkins | 1996 | IEEE1996，，： | 1 |
| 16 | A meta-analysis on the anxiety-reducing effects of acute and chronic exercise显示文摘 | PETRUZZELLO S J LANDERS D M HATFIELD B D | 1991 | Sports Med1991，11，3： | 1 |
| 17 | Selenocysteine incorpo?ration machinery and the role of selenoproteins in development and health 显示文摘 | Hatfield DL Carlson BA Xu XM | 2006 | Prog Nucleic Acid Res Mol Bioi2006，81，1： | 1 |
| 18 | Passionate love and anxiety in young adolescents显示文摘 | Elaine Hatfield Connie Brinton Jeffrey Cornelius | 1989 | Motivation and Emotion1989，，4： | 1 |
| 19 | An Empirical Examination ofthe Relationship between Corporate Social P~esponsibilfty and Profitability 显示文摘 | Aupperle K E Carroll A B Hatfield J D | | Academy of Management Journal0，985，： | 1 |
| 20 | The MicroRNA pathway plays a regulatory role in stem cell division 显示文摘 | Shcherbata HR Hatfield S Ward EJ | 2006 | Cell Cycle2006，5，2： | 1 |
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