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| 1 | Epidemiology of Diabetes in Adults Aged 35 and Older from Shanghai,China显示文摘Objective This study aimed to investigate the epidemiology of diabetes in adults from Shanghai, aged 35 and older, in 2013. Methods We estimated the diabetes prevalence in a representative sample of 18,736 adults who were selected through a multistage stratified cluster sampling process. A standard questionnaire containing questions about demographic characteristics and lifestyle factors was distributed. After an overnight fast of at least 10 hours, a venous blood sample was collected from each participant. For each patient without a history of diabetes, another blood sample was drawn 120 min after an oral glucose tolerance test to identify undiagnosed diabetes and prediabetic condition. Results Among Shanghai residents aged 35 and above, the overall weighted prevalence of diabetes was 17.6% [95% confidence interval(CI): 16.4%-18.8%]. The prevalences were 19.3% in men and 15.8% in women as well as 19.1%, 15.4%, and 16.1% in urban, suburban, and rural residents, respectively. In addition, the weighted prevalence of prediabetes was 16.5%(95% CI: 15.3%-17.8%), with the prevalences of 16.5% in men, 16.6% in women, 15.2% in urban residents, 18.0% in suburban residents, and 18.5% in rural residents. Among all patients with diabetes, 68.1%(95% CI: 64.3%-71.6%) were aware of their status, 63.5%(95% CI: 60.0%-66.9%) received diabetes treatment, but only 35.1%(95% CI: 32.4%-37.8%) had adequate glycemic control. Conclusion In Shanghai, diabetes and prediabetes are highly prevalent. However, 1/3rd of diabetes cases are undiagnosed, and the rate of glycemic control is low. | RUAN Ye YAN Qing Hua XU Ji Ying YANG Qun Di YAO Hai Hong LI Rui SHI Yan | 2016 | Biomedical and Environmental Sciences2016，29，6： | 22 |
| 2 | Molecular Epidemiological Characteristics of Streptococcus pyogenes Strains Involved in an Outbreak of Scarlet Fever in China,2011显示文摘Objective To investigate molecular characterization of streptococcus pyogenes isolates involved in an outbreak of scarlet fever in China in 2011.Methods Seventy-four Streptococcal pyogenes involved in an outbreak of scarlet fever were isolated from pediatric patients in the areas with high incidence in China from May to August of 2011.Emm genotyping,pulsed-field gel electrophoresis (PFGE),superantigen (SAg) genes and antimicrobial susceptibility profiling were analyzed for these isolates.Results A total of 4 different emm types were identified.Emm12 was the most prevalent type which contained four predominating PFGE patterns corresponding to four different virulence and superantigen profiles.Emm12 (79.7%) and emml (14.9%) accounted for approximately 94%of all the isolates.The speA gene was all negative in emm12 isolates and positive in emml isolates.All strains were resistant to erythromycin,and 89.4%of them were resistant to erythromycin,tracycline,and clindamycin simultaneously.Conclusion Several highly diversified clones with a high macrolide resistance rate comprise a predominant proportion of circulating strains,though no new emm type was found in this outbreak.The data provide a baseline for further surveillance of scarlet fever,which may contribute to the explanation of the outbreak and development of a GAS vaccine in China. | YOU Yuan Hai SONG Yan Yan YAN Xiao Mei WANG Hai Bin ZHANG Meng Han TAO Xiao Xia Li Lei Lei ZHANG Yu Xin JIANG Xi Hong ZHANG Bing Hua ZHOU Hao XIAO Di JIN Lian Mei FENG Zi Jian LUO Feng Ji ZHANG Jian Zhong | 2013 | Biomedical and Environmental Sciences2013，26，11： | 15 |
| 3 | Over-expression of LPTS-Lin hepatocellular carcinoma cell line SMMC-7721 induces crisis显示文摘AIM: To evaluate the function of the longer transcripts LPTS-Lin hepatocellular carcinoma cell line SMMC-7721.METHODS: SMMC-7721 cells were transfected with LPTSL expression construct and stably transfected cells were selected by G418. Multiple single clones formed and were checked for their phenotype. In the study of the effect on telomerase activity of LPTS-Lin vitro, GST-LPTS-L fusion protein was expressed in E.coli and purified by glutathioneagarose column. Telomeric repeat amplification protocol (TRAP) assays were performed to study the influence of telomerase activity in SMMC-7721 cells.RESULTS: Over-expression of LPTS-L induced SMMC-7721 cells into crisis. LPTS-L could inhibit the telomerase activity in SMMC-7721 cellsin vitro.CONCLUSION: LPTS-L is a potent telomeraseinhibitor. Over-expression of LPTS-L can induce hepatoma cells into crisis due to the reduction of telomerase activity. | Cheng Liao Mu-Jun Zhao Jing Zhao Di Jia Hai Song Zai-Ping Li,State Key Laboratory of Molecular Biology,Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China | 2002 | World Journal of Gastroenterology2002，8，6： | 9 |
| 4 | Receptome profiling identifies KREMEN1 and ASGR1 as alternative functional receptors of SARS-CoV-2显示文摘Host cellular receptors play key roles in the determination of virus tropism and pathogenesis.However,little is known about SARS-CoV-2 host receptors with the exception of ACE2.Furthermore,ACE2 alone cannot explain the multi-organ tropism of SARS-CoV-2 nor the clinical differences between SARS-CoV-2 and SARS-CoV,suggesting the involvement of other receptor(s).Here,we performed genomic receptor profiling to screen 5054 human membrane proteins individually for interaction with the SARS-CoV-2 capsid spike(S)protein.Twelve proteins,including ACE2,ASGR1,and KREMEN1,were identified with diverse S-binding affinities and patterns.ASGR1 or KREMEN1 is sufficient for the entry of SARS-CoV-2 but not SARS-CoV in vitro and in vivo.SARS-CoV-2 utilizes distinct ACE2/ASGR1/KREMEN1(ASK)receptor combinations to enter different cell types,and the expression of ASK together displays a markedly stronger correlation with virus susceptibility than that of any individual receptor at both the cell and tissue levels.The cocktail of ASK-related neutralizing antibodies provides the most substantial blockage of SARS-CoV-2 infection in human lung organoids when compared to individual antibodies.Our study revealed an interacting host receptome of SARS-CoV-2,and identified ASGR1 and KREMEN1 as alternative functional receptors that play essential roles in ACE2-independent virus entry,providing insight into SARS-CoV-2 tropism and pathogenesis,as well as a community resource and potential therapeutic strategies for further COVID-19 investigations. | Yunqing Gu Jun Cao Xinyu Zhang Hai Gao Yuyan Wang Jia Wang Juan He Xiaoyi Jiang Jinlan Zhang Guanghui Shen Jie Yang Xichen Zheng Gaowei Hu Yuanfei Zhu Shujuan Du Yunkai Zhu Rong Zhang Jianqing Xu Fei Lan Di Qu Guoliang Xu Yun Zhao Dong Gao Youhua Xie Min Luo Zhigang Lu | 2022 | Cell Research2022，32，1： | 8 |
| 5 | Distinct immune signatures discriminate between asymptomatic and presymptomatic SARS-CoV-2^(pos)subjects显示文摘Increasing numbers of SARS-CoV-2-positive(SARS-CoV-2^(pos))subjects are detected at silent SARS-CoV-2 infection stage(SSIS).Yet,SSIS represents a poorly examined time-window wherein unknown immunity patterns may contribute to the fate determination towards persistently asymptomatic or overt disease.Here,we retrieved blood samples from 19 asymptomatic and 12 presymptomatic SARS-COV-2^(pos)subjects,47 age/gender-matched patients with mild or moderate COVID-19 and 27 normal subjects,and interrogated them with combined assays of 44-plex CyTOF,RNA-seq and Olink.Notably,both asymptomatic and presymptomatic subjects exhibited numerous readily detectable immunological alterations,while certain parameters including more severely decreased frequencies of CD107a^(low)classical monocytes,intermediate monocytes,non-classical monocytes and CD62L^(hi)CD8^(+)Tnaive cells,reduced plasma STC1 level but an increased frequency of CD4^(+)NKT cells combined to distinguish the latter.Intercorrelation analyses revealed a particular presymptomatic immunotype mainly manifesting as monocytic overactivation and differentiation blockage,a likely lymphocyte exhaustion and immunosuppression,yielding mechanistic insights into SSIS fate determination,which could potentially improve 5ARS-CoV-2 management. | Shanhe Yu Caixia Di Shijun Chen Mingquan Guo Jiayang Yan Zhaoqin Zhu Li Liu Ruixue Feng Yinyin Xie Ruihong Zhang Juan Chen Mengxi Wang Dong Wei Hai Fang Tong Yin Jinyan Huang Saijuan Chen Hongzhou Lu Jiang Zhu Jieming Qu | 2021 | Cell Research2021，31，11： | 4 |
| 6 | Bifurcations of Limit Cycles in a Z_4-Equivariant Quintic Planar Vector Field显示文摘在这份报纸, Z 4-equivariant quintic 平面向量地被学习。Hopf 分叉方法和 polycycle 分叉方法被联合学习与 4 个夸张僵绳点从加重的周期区分的限制周期。它被发现那平面多项式系统有的这特殊 quintic 包围所有单个点的至少四个大限制周期。由使用双 homoclinic 环分叉方法和 Hopf 分叉方法,我们断定有二种不同配置的 28 个限制周期在这个特殊平面多项式系统存在。在这份报纸获得的结果为学习是有用的削弱了第 16 Hilbert 鈥檚 问题。加重的关键词骑车-两倍 homoclinic 环-稳定性-分叉-限制周期-限制周期先生( 2000 )题目分类 34C07 的分发- 34C23 - 34C37 - 37G15 由江苏大学(资助号码 05JDG011 )和中国的国家自然科学基础的青春的资金支持了(资助号码 10771088 ) | Yu Hai WU Xue Di WANG Li Xin TIAN | 2010 | Acta Mathematica Sinica,English Series2010，26，4： | 3 |
| 7 | Fluorescence polarization assay improves the rapid detection of human brucellosis in China显示文摘Background:Brucellosis is an infectious-allergic zoonotic disease caused by bacteria of the genus Brucella.Early diagnosis is the key to preventing,treating,and controlling brucellosis.Fluorescence polarization immunoassay(FPA)is a new immunoassay for relatively rapid and accurate detection of antibodies or antigens based on antigen-antibody interaction.However,there is no report on FPA-based detection of human brucellosis in China.Therefore,this study is to evaluate the value of FPA for the diagnosis of human brucellosis in China. | Shuai-Bing Dong Di Xiao Jing-Yao Liu Hui-Mei Bi Zun-Rong Zheng Li-Da Wang Xiao-Wen Yang Guo-Zhong Tian Hong-Yan Zhao Dong-Ri Piao Zhi-Feng Xing Hai Jiang | 2021 | Infectious Diseases of Poverty2021，10，2： | 3 |
| 8 | BMI and BMI Changes to All-cause Mortality among the Elderly in Beijing: a 20-year Cohort Study显示文摘Objective To explore the association between body mass index(BMI)and all-cause mortality among the elderly in Beijing.Methods This analysis was based on the Beijing multidimensional longitudinal study of aging(BLSA),which included 2,090 subjects over 55 years old and was followed-up from 1992 to 2012.BMI-mortality curves were drawn to find the optimal BMI range with the lowest mortality.Cox proportional hazard models were used to obtain the hazard ratios(HRs)for BMI and BMI changes in the overall population and in specific stratified populations.Results During follow-up,1,164 deaths were recorded;BMI-mortality curve was U-shaped,with the lowest mortality at a BMI of approximately 25 kg/m^2.After adjusting for gender,age,smoking,drinking and some pre-existing diseases,HRs for underweight,overweight and obesity compared with normal weight were 1.372(95%CI:1.154-1.631),0.767(95%CI:0.666-0.884)and 0.871(95%CI:0.830-1.246),respectively.HR for BMI drop was 3.245(95%CI:0.824-12.772)in the underweight group and 1.892(95%CI:0.830-1.246)in the normal weight group,HR for BMI rise was 1.795(95%CI:1.243-2.591)in normal weight group and 1.962(95%CI:1.202-3.203)in the overweight group.Conclusion Keeping BMI in an overweight status and stable is related to a reduced mortality. | WANG Yun Feng TANG Zhe GUO Jin TAO Li Xin LIU Long LI Hai Bin LI Di Tian GUO Xiu Hua YANG Xing Hua | 2017 | Biomedical and Environmental Sciences2017，30，2： | 3 |
| 9 | Hydrogen Therapy Reduces Oxidative Stress-associated Risks Following Acute and Chronic Exposure to High-altitude Environment显示文摘Low pressure,low oxygen concentration,and intense ultraviolet(UV)radiation in high-altitude environments,can cause oxidative stress which can trigger mountain sickness.A recent study demonstrated that hydrogen gas with a good permeability in biological membranes can treat various disorders by exerting its selective anti-oxidation and anti-inflammatory effects,indicating that hydrogen therapy plays a role | SHI Qing Hai WEI Wei RAN Ji Hua WANG Si Yuan LIU Zheng Xiang GE Di CHEN Ping FU Jian Feng | 2015 | Biomedical and Environmental Sciences2015，28，3： | 3 |
| 10 | The MING proposal at SHINE:megahertz cavity enhanced X‑ray generation显示文摘The cavity-based X-ray free-electron laser(XFEL)has promise in producing fully coherent pulses with a bandwidth of a few meV and very stable intensity,whereas the currently existing self-amplified spontaneous emission(SASE)XFEL is capable of generating ultra-short pulses with chaotic spectra.In general,a cavity-based XFEL can provide a spectral brightness three orders of magnitude higher than that of the SASE mode,thereby opening a new door for cutting-edge scientific research.With the development of superconducting MHz repetition-rate XFEL facilities such as FLASH,European-XFEL,LCLS-II,and SHINE,practical cavity-based XFEL operations are becoming increasingly achievable.In this study,megahertz cavity enhanced X-ray generation(MING)is proposed based on China’s first hard XFEL facility-SHINE,which we refer to as MING@SHINE. | Nan‑Shun Huang Zi‑Peng Liu Bang‑Jie Deng Zi‑Han Zhu Shao‑Hua Li Tao Liu Zheng Qi Jia‑Wei Yan Wei Zhang Sheng‑Wang Xiang Yang‑Yang Lei Ya Zhu Yong‑Zhou He Qi‑Bing Yuan Fei Gao Rong‑Bing Deng Sen Sun Zhi‑Di Lei Zhi‑Qiang Jiang Meng‑Qi Duan Yuan Zhuan Xue‑Fang Huang Peng‑Cheng Dong Zhong‑Liang Li Shang‑Yu Si Lian Xue Si Chen Yong‑Fang Liu Ya‑Jun Tong Hai‑Xiao Deng Zhen‑Tang Zhao | 2023 | Nuclear Science and Techniques2023，34，1： | 2 |
| 11 | Microscopic insight into the single step growth of in-plane heterostructures between graphene and hexagonal boron nitride显示文摘Graphene-h-BN hybrid nanostructures are grown in one step on the Pt(111) surface by ultra-high vacuum chemical vapor deposition using a single precursor,the dimethylamino borane complex.By varying the deposition conditions,different nanostructures ranging from a fully continuous hybrid monolayer to well-separated Janus nanodots can be obtained.The growth starts with heterogeneous nucleation on morphological defects such as Pt step edges and proceeds by the addition of small clusters formed by the decomposition of the dimethylamino borane complex.Scanning tunneling microscopy measurements indicate that a sharp zigzag in-plane boundary is formed when graphene grows aligned with the Pt substrate and consequently with the h-BN layer as well.When graphene is rotated by 30°,the graphene armchair edges are seamlessly connected to h-BN zigzag edges.This is confirmed by a thorough density functional theory (DFT) study.Angle resolved photoemission spectroscopy (ARPES) data suggests that both h-BN and graphene present the typical electronic structure of self-standing non-interacting materials. | Thanh Hai Nguyen Daniele Perilli Mattia Cattelan Hongsheng Liu Francesco Sedona Neil A. Fox Cristiana Di Valentin Stefano Agnoli | 2019 | Nano Research2019，12，3： | 2 |
| 12 | Rapid and High-throughput Identification of Recombinant Bacteria with Mass Spectrometry Assay显示文摘Objective To construct a rapid and high-throughput assay for identifying recombinant bacteria based on mass spectrometry.Methods Matrix assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS) techniques were used to identify 12 recombinant proteins(10 of Yersinia pestis,1 of Campylobacter jejuni and 1 of Helicobacter pylori).A classification model for the various phase of recombinant bacteria was established,optimized and validated,using MALDI-TOF MS-ClinProTools system.The differences in the peptide mass spectra were analyzed by using Biotyper and FlexAnalysis softwares.Results Models of GA,SNN,and QC were established.After optimizing the parameters,the GA recognition model showed good classification capabilities:RC=100%,mean CVA=98.7%(the CVA was96.4%in phase 1,100%in phase 2,98.4%in phase 3,and 100%in phase 4,respectively) and PPV=95%.This model can be used to classify the bacteria and their recombinant,which only requires 3.7xl03 cells for analysis.The total time needed is only 10 min from protein extraction to reporting the result for one sample.Furthermore,this assay can automatically detect and test 96 samples concurrently.A total of 48specific peaks(9,16,9,and 14 for the four stages,respectively) was found in the various phase of recombinant bacteria.Conclusion MALDI-TOF MS can be used as a fast,accurate,and high-throughput method to identify recombinant bacteria,which provide a new ideas not only for recombinant bacteria but also for the identification of mutant strains and bioterrorism pathogens. | XIAO Di TAO Xiao Xia WANG Peng LIU Guo Dong GONG Ya Nan ZHANG Hui Fang WANG Hai Bin ZHANG Jian Zhong | 2014 | Biomedical and Environmental Sciences2014，27，4： | 2 |
| 13 | Dynamic changes of telomeric restriction fragment (TRF) lengths in cells during the developmental process from embryos to seedlings and a comparison with the embryonal calli in Ginkgo biloba L.显示文摘Telomeres are the structures that locate at the terminals of linear eukaryotic chromosomes. They can play essential roles in many cellular processes. The terminal location of Arabidopsis-type TTTAGGG tandem repeats were thought to be highly conserved. The terminal location of Ginkgo biloba L. consisting of TTTAGGG tandem repeats, were confirmed by Bal31 exonuclease degradation and Southern blotting. By comparing telomeric restriction fragment (TRF) lengths at different developmental stages from embryos to seedlings, a fluctuant tendency towards variation was found in these samples. The TRF length of embryos was also compared with that of embryonal calli and an upward trend was discovered in callus culture. The results suggest that there should be a telomerase mechanism or/and ALT mechanism for the maintenance of telomere length. | Liu Di Zhang Xiao-mei Hua Xin Qiao Nan Song Han Lu Hai Guo Hui-hong Li Feng-lan | 2007 | Forestry Studies in China2007，9，2： | 2 |
| 14 | In Vitro Protein Expression Profile of Campylobacter jejuni Strain NCTC11168 by Two-dimensional Gel Electrophoresis and Mass Spectrometry显示文摘Objective To investigate the protein expression profiles of the major food‐borne pathogen Campylobacter jejuni NCTC11168.Methods Membrane and soluble cellular proteins were extracted from the genome‐sequenced C.jejuni strain NCTC11168.Protein expression profiles were determined using two‐dimensional gel electrophoresis(2‐DE).All the detected spots on the 2‐DE map were subjected to matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry(MALDI‐TOF/TOF) analysis.Results A total of 537 and 333 spots were detected from the whole cell and membrane‐associated proteins of C.jejuni NCTC11168 cultured on Columbia agar medium at 42 ℃ by 2‐DE and Coomassie Brilliant Blue staining,respectively.Analyses of whole cell and membrane‐associated proteins included 399 and 133 spots,respectively,which included 182 and 53 functional proteins identified by MALDI‐TOF/TOF analysis.Conclusion The comprehensive expression protein profiles of C.jeuni NCTC11168 obtained in this study will be useful for elucidating the roles of these proteins in further pathogenesis investigation. | ZHANG Mao Jun GU Yi Xin DI Xiao ZHAO Fei YOU Yuan Hai MENG Fan Liang ZHANG Jian Zhong | 2013 | Biomedical and Environmental Sciences2013，26，1： | 2 |
| 15 | A Survey of Non-Volatile Main Memory Technologies:State-of-the-Arts,Practices,and Future Directions显示文摘Non-Volatile Main Memories (NVMMs) have recently emerged as a promising technology for future memory systems. Generally, NVMMs have many desirable properties such as high density, byte-addressability, non-volatility, low cost, and energy efficiency, at the expense of high write latency, high write power consumption, and limited write endurance. NVMMs have become a competitive alternative of Dynamic Random Access Memory (DRAM), and will fundamentally change the landscape of memory systems. They bring many research opportunities as well as challenges on system architectural designs, memory management in operating systems (OSes), and programming models for hybrid memory systems. In this article, we revisit the landscape of emerging NVMM technologies, and then survey the state-of-the-art studies of NVMM technologies. We classify those studies with a taxonomy according to different dimensions such as memory architectures, data persistence, performance improvement, energy saving, and wear leveling. Second, to demonstrate the best practices in building NVMM systems, we introduce our recent work of hybrid memory system designs from the dimensions of architectures, systems, and applications. At last, we present our vision of future research directions of NVMMs and shed some light on design challenges and opportunities. | Hai-Kun Liu Di Chen Hai Jin Xiao-Fei Liao Binsheng He Kan Hu Yu Zhang | 2021 | Journal of Computer Science & Technology2021，36，1： | 2 |
| 16 | Efficient production of pronuclear embryos in breeding and nonbreeding season for generating transgenic sheep overexpressing TLR4显示文摘Background: Brucella is a zoonotic Gram-negative pathogen that causes abortion and infertility in ruminants and humans. TLR4 is the receptor for LPS which can recognize Brucella and initiate antigen-presenting cell activities that affect both innate and adaptive immunity. Consequently, transgenic sheep over-expressing TLR4 are an suitable model to investigate the effects of TLR4 on preventing Brucellosis. In this study, we generated transgenic sheep overexpressing TLR4 and aimed to evaluate the effects of different seasons(breeding and non-breeding season) on superovulation and the imported exogenous gene on growth.Results: In total of 43 donor ewes and 166 recipient ewes in breeding season, 37 donor ewes and 144 recipient ewes in non-breeding season were selected for super-ovulation and injected embryo transfer to generate transgenic sheep.Our results indicated the no. of embryos recovered of donors and the rate of pronuclear embryos did not show any significant difference between breeding and non-breeding seasons(P > 0.05). The positive rate of exogenous TLR4 tested were 21.21 % and 22.58 % in breeding and non-breeding season by Southern blot. The expression level of TLR4 in the transgenic sheep was 1.5 times higher than in the non-transgenic group(P < 0.05). The lambs overexpressing TLR4 had similar growth performance with non-transgenic lambs, and the blood physiological parameters of transgenic and non-transgenic were both in the normal range and did not show any difference.Conclusions: Here we establish an efficient platform for the production of transgenic sheep by the microinjection of pronuclear embryos during the whole year. The over-expression of TLR4 had no adverse effect on the growth of the sheep. | Yan Li Di Lian Shoulong Deng Xiaosheng Zhang Jinlong Zhang Wenting Li Hai Bai Zhixian Wang Hongping Wu Juncai Fu Hongbing Han Jianzhong Feng Guoshi Liu Ling Lian Zhengxing Lian | 2017 | Journal of Animal Science and Biotechnology2017，8，1： | 2 |
| 17 | Structure andProperty Analysis of Ceramic Coating 显示文摘 | Li hai lin Wu dong di | 1997 | Huadong ligongdaxue xuebao1997，23，2： | 1 |
| 18 | Investigation of Microstructure and Tribological Properties of CrTil _ xN Com- posite Films on Piston Rings 显示文摘 | Zhi Hai Cai Yue Ian Di Jia Wu He | 2011 | Advanced Materials Re- search2011，，： | 1 |
| 19 | Disorders of lipid metabolism in muscle显示文摘 | Di MS Trevisan C Hays A | 1980 | Muscle Nerve1980，3，5： | 1 |
| 20 | Exon skipping of AGAMOUS homolog PrseAG in developing double flowers of Prunus lannesiana (Rosaceae)显示文摘 | Zhixiong Liu Dandan Zhang Di Liu Fenglan Li Hai Lu | | Plant Cell Reports0，，： | 1 |
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