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1555篇 您的检索式:作者名="Juan Chen"
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1Reappraisement and refinement of zircon U-Pb isotope and trace element analyses by LA-ICP-MS显示文摘A protocol was established for simultaneous measurements of zircon U-Pb ages and trace elements by LA-ICP-MS at spot sizes of 16 32 μm.This was accomplished by introducing N 2 into ICP to increase the sensitivity.The obtained U-Pb ages for zircon standards GJ-1,TEMORA and SK10-2 are consistent with the preferred values within about 1% uncertainty (2σ) by simple external calibration against zircon standard 91500.Different data reduction softwares could yield different uncertainties for calculation of U-Pb ages.The commercially available program GLITTER4.4 could apply an improper uncertainty calculation strategy,but it may yield artificial high precisions for single analyses.Our trace element analyses indicate that Si is not an ideal internal standard for zircon when calibrated against the NIST glasses.Calibration against the NIST glasses using Si as an internal standard,a systematic deviation of 10% 30% was found for most trace elements including Zr.However,the trace element compositions of zircon can be accurately measured by calibration against multiple reference materials with natural compositions (e.g.,BCR-2G,BHVO-2G and BIR-1G),or calibration against NIST SRM 610 and using Zr as an internal standard.Analyses of two pieces of GJ-1 demonstrate that it is relatively homogenous for most trace elements (except for Ti).LIU YongSheng HU ZhaoChu ZONG KeQing GAO ChangGui GAO Shan XU Juan CHEN HaiHong 2010Chinese Science Bulletin2010,55,15:1011
2New insights into the roles of CHOP-induced apoptosis in ER stress显示文摘Endoplasmic 蜂窝胃应力(嗯应力) 在 ER 由于动态平衡的损失被触发,在 ER 腔导致累积 ofmisfolded 蛋白质。嗯因为 ER 的展开的蛋白质 response.Perturbation 是抄写因素 C/EBP 相应蛋白质(砍) 的强大的 inducer,应力激活知道的一系列适应机制。尽管它过时的人或物证明到 ER 的过多或不利的应力触发 apoptosis,由砍位于这些 processesinduced 下面的特定的机制仍然保持不清楚。现在,导致砍的 apoptosis 在嗯应力在众多的人的疾病被含有例如 neurodegenerative 疾病,糖尿病, ischemic 疾病,肿瘤等等。在这评论,我们从实验室在几疾病的发展总结了砍的角色的 currentunderstanding 到诊所。Yiming Li Yunshan Guo Juan Tang Jianli Jiang Zhinan Chen 2014Acta Biochimica et Biophysica Sinica2014,46,8:45
3Current Trends of the Prevalence of Childhood Asthma in Three Chinese Cities: A Multicenter Epidemiological Survey显示文摘Objective To analyze the prevalence of asthma and asthma related symptoms among children aged 0-14 years in three Chinese cities and to obtain a crude estimation of the trend of childhood asthma prevalence in China. Methods A cross-sectional, population-based survey of prevalence of asthma was conducted in children aged from 0 to 14 years in 3 major cities of China (Beijing, Chongqing, and Guangzhou) with different geographic locations. All the subjects were randomly selected by a multi-stage sampling method. Three to five schools and kindergartens in 2 urban districts in each city were randomly selected for the survey, and a validated questionnaire that included the core questions of the International Study of Asthma and Allergies in Childhood, Phase Ⅲ questionnaire and several additional questions were used. All questionnaires were completed by parents or guardians of the selected children. Children whose parents responded affirmatively to the question' Has your child ever been diagnosed as asthma by a doctor' were recognized as victims of asthma. Results The prevalence of asthma in Beijing, Chongqing, and Guangzhou was 3.15%, 7.45%, and 2.09%, respectively. These values were significantly higher than those obtained 10 years ago in the national epidemiological survey in 2000 which used the same method of investigation and the same diagnotic criteria (χ2 =3.938, P=0.047; χ2 =73.506, P≤0.001; χ2 =11.956, P=0.001, in each city). Of the asthmatic children 57.21%, 69.91%, and 60.00% had their first attack before the age of 3 in Beijing, Chongqing, and Guangzhou, respectively. Wheezing was the primary clinical manifestation for all asthmatic children , followed by persistent cough and repeated respiratory infections. Both the prevalence of asthma and asthma-related symptoms were statistically higher in males than in females. Conclusion The prevalence of childhood asthma is statistically higher than that 10 years ago in the three Chinese cities.JUAN BAI JiNG ZHAO KUN-LING SHEN LI XIANG AI-HUAN CHEN SUI HUANG YING HUANG JIAN-SHENG WANG RONG-WEI YE 2010Biomedical and Environmental Sciences2010,23,6:37
4Locking plate fixation combined with iliac crest bone autologous graft for proximal humerus comminuted fracture显示文摘Zhu Lian Liu Yueju Yang Zongyou Li Han Wang Juan Zhao Changping Chen Xiao Zhang Yingze 2014Chinese Medical Journal2014,,9:40
5IL-23 signaling enhances Th2 polarization and regulates allergic airway inflammation显示文摘Juan Peng Xuexian O Yang Seon Hee Chang Jiong Yang Chen Dong 2010Cell Research2010,20,1:31
6Genetic correction of p-thalassemia patient-specific iPS cells and its use in improving hemoglobin production in irradiated SCID mice显示文摘Yixuan Wang Chen-Guang Zheng Yonghua Jiang Jiqin Zhang Jiayu Chen Chao Yao Qingguo Zhao Sheng Liu Ke Chen Juan Du Ze Yang Shaorong Gao 2012Cell Research2012,22,4:27
7Research of the Unity Theory Between Three-level Space Vector and Carrier-based PWM Modulation Strategy显示文摘两电平空间矢量脉宽调制(space vector pulse widthmodulation,SVPWM)与正弦脉宽调制(sinusoidal pulse widthmodulation,SPWM)存有一定的本质关系,对于三电平,在常见的8段式SVPWM序列中可以证明,SVPWM与SPWM本质上也存在某种统一。实际的SVPWM调制中,由于要考虑中点电压平衡、减少谐波等其它性能问题,一般会利用开关冗余状态得到8段以上的开关调制序列。目前的文献中还未曾有对这些情况进行统一性地验证,因此三电平SVPWM与SPWM的统一理论存在一定的不足。研究发现,可通过严格地理论推导将传统意义上的调制波分解为2个子调制波,再进行SPWM调制,可以得到与SVPWM完全吻合的序列。该文在此方法基础上深入研究了8段以上(10、12、14段)的SVPWM调制序列与SPWM的统一。经过严格地理论推导,成功实现了含任意段数的SVPWM序列与SPWM的统一。仿真结果证明了该理论的正确性。CHEN Juan HE Yingjie WANG Xinyu LIU Jinjun 2013中国电机工程学报2013,33,9:28
8Rhizospheric microbial communities are driven by Panax ginseng at different growth stages and biocontrol bacteria alleviates replanting mortality显示文摘The cultivation of Panax plants is hindered by replanting problems, which may be caused by plantdriven changes in the soil microbial community. Inoculation with microbial antagonists may efficiently alleviate replanting issues. Through high-throughput sequencing, this study revealed that bacterial diversity decreased,whereas fungal diversity increased, in the rhizosphere soils of adult ginseng plants at the root growth stage under different ages. Few microbial community, such as Luteolibacter, Cytophagaceae, Luteibacter, Sphingomonas,Sphingomonadaceae, and Zygomycota, were observed; the relative abundance of microorganisms, namely,Brevundimonas, Enterobacteriaceae, Pandoraea, Cantharellales, Dendryphion, Fusarium, and Chytridiomycota,increased in the soils of adult ginseng plants compared with those in the soils of 2-year-old seedlings. Bacillus subtilis 50-1, a microbial antagonist against the pathogenic Fusarium oxysporum, was isolated through a dual culture technique. These bacteria acted with a biocontrol efficacy of 67.8%. The ginseng death rate and Fusarium abundance decreased by 63.3% and 46.1%, respectively, after inoculation with B. subtilis 50-1. Data revealed that microecological degradation could result from ginseng-driven changes in rhizospheric microbial communities;these changes are associated with the different ages and developmental stages of ginseng plants. Biocontrol using microbial antagonists alleviated the replanting problem.Linlin Dong Jiang Xu Lianjuan Zhang Ruiyang Cheng Guangfei Wei He Su Juan Yang Jun Qian Ran Xu Shilin Chen 2018Acta Pharmaceutica Sinica B2018,8,2:27
9Effect of a cancer vaccine prepared by fusions of hepatocarcinoma cells with dendritic cells显示文摘AIM To prepare a cancer vaccine (H22-DC) expressing high levels of costimulatory molecules based on fusions of hepatocarcinoma cells ( H22 ) with dendritic cells (DC) of mice and to analyze the biological characteristics and induction of specific CTL activity of H22-DC.``METHODS DCs were isolated from murine spleen by metrizamide density gradient centrifugation, purified based on its characteristics of semi-adhesion to culture plates and FcR , and were cultured in the medium containing GM. CSF and IL-4. A large number of DC were harvested. DCs were then fused with H22 cells by PEG and the fusion cells were marked with CDllc MicroBeads. The H22-DC was sorted with Mimi MACS sorter. The techniques of cell culture, immunocytochemistry and light microscopy were also used to test the characteristice of growth and morphology of H22-DC in vitro. As the immunogen, H22-DC was inoculated subcutaneously into the right armpit of BALB/C mice, and their tumorigenicity in vive was observed. MTT was used to test the CTL activity of murine spleen in vitro.``RESULTS DC cells isolated and generated were CD1 lc +cells with irregular shape, and highly expressed CD80,CD86 and CD54 molecules. H22 cells were CDllc cells with sphericel shape and bigger volume, and did not express CD80, CD86 and CD54 molecules. H22-DC was CDllc+ cells with bigger volume, being spherical, flat or irregular in shape, and highly expressed CD80, CD86 and CD54 molecules, too. H22-DC was able to divide and proliferate in vitro, but its activity of proliferation was significantly decreased as compared with H22 cells and its growth curve was flatter than H22 cells. After subcutaneous inoculation over 60 days, 1-12_2-DC showed no tumorigenecity in mice, which was significantly different from control groups (P< 0.01 ) . The spleen CTL activity against H22 cells in mice implanted with fresh H22-DC was significantly higher than control groups (P< 0.0l).``CONCLUSION H22-DC could significantly stimulate the specific CTL activity of murine spleen, which suggests that the fusion cells have already obtained the function of antigen presenting of parental DC and could present H22specific antigen which has not been identified yet, and H22-DC could induce antitumor immune response; although simply mixed H22 cells with DC could stimulate the specific CTL activity which could inhibit the growth of tumor in some degree, it could not prevent the generation of tumor. It shows that the DC vaccine is likely to become a helpful approach in immunotherapy of hepstocarcinoma.Juan Zhang~1 Jin-Kun Zhang~2 Shao-Hong Zhuo~3 Hai-Bin Chen~2 1 Clinical Laboratory,The First Affiliated Hospital of Shantou University Medical College,Shantou 515041,Guangdong Province,China2 Cancer Pathology Laboratory,Shantou University Medical College,Shantou 515031,Guangdong Province,China3 Department of Gastroenterology,Third Municipal Hospital of Shantou,Shantou 515073,Guangdong Province,China 2001World Journal of Gastroenterology2001,7,5:26
10Clinical Characteristics of Patients with Diabetic Nephropathy on Maintenance Hemodialysis: A Multicenter Cross-sectional Survey in Anhui Province, Eastern China显示文摘Hu Chen De-Guang Wang Liang Yuan Gui-Ling Liu Heng-Jie He Juan Wang Sen Zhang Li Hao 2016Chinese Medical Journal2016,,11:24
11Effect of three interventional bronchoscopic methods on tracheal stenosis and the formation of granulation tissues in dogs显示文摘为繁的使成粒状纸巾的形成引起的气管的狭窄的背景治疗学的途径通常包括 electrocautery,机械膨胀,激光治疗,氩血浆凝结(APC ) ,冷冻疗法和 stent 放置。然而,在 stent 插入以后的狭窄仍然是重要限制。我们检验了三条不同途径的功效,包括机械损害的正式就职, APC 与不同力量或持续时间和冷冻疗法表现了,识别限制了使成粒状织物的形成的方法。12 特殊饲养了研究杂种狗的方法被划分成三个组。在组 1 (四条狗) 温和(过程 1;二条狗) 或中等(过程 2;二条狗) 损坏机械地被导致。(六条狗) 组 2 在不同力量或持续时间收到了 APC (过程 3:30 W, 1 amis;过程 4:30 W, 2 cm/s;过程 5:25 W, 3 cm/s ) 。(二条狗) 组 3 收到了冷冻疗法(过程 6:30 秒的二个结冰融化周期) 。揭开的自我可扩充的金属性的 stents 在所有狗被插入维持连续刺激到气管 mucosa。狗被监视因为在使用的使成粒状织物增长和方法之间的 4 个星期和关系被分析。在组 1 的结果,使成粒状织物生长与更多的严重机械损坏增加了。在组 2 的使成粒状织物的生长比在组 1 更显著,并且因为严重狭窄,在过程 3 的两条狗死了。在这个组,使成粒状织物的形成与减少的力量和持续时间减少了。在组 3,没有明显的使成粒状织物在星期 4 点被发现。从外国身体(stent ) 的结论损害和刺激是导致使成粒状组织的增生的二个重要因素。热损害例如 APC,似乎导致更大的使成粒状织物生长和软骨损坏与相比机械并且冷冻疗法损害。在有机械膨胀的联合的冷冻疗法可以是为管理使成粒状织物的形成引起的气管的狭窄的一个安全、有效的处理方法。ZHANG Jie WANG Ting WANG Juan PEI Ying-hua XU Min WANG Yu-ling ZHANG Xia WANG Chen 2010Chinese Medical Journal2010,,5:23
12A Simple and Rapid Colloidal Gold-based Immunochromatogarpic Strip Test for Detection of FMDV Serotype A显示文摘A sandwich format immunochromatographic assay for detecting foot-and-mouth disease virus (FMDV) serotypes was developed. In this rapid test,affinity purified polyclonal antibodies from Guinea pigs which were immunized with sucking-mouse adapted FMD virus (A/AV88(L) strain) were conjugated to colloidal gold beads and used as the capture antibody,and affinity purified polyclonal antibodies from rabbits which were immunized with cell-culture adapted FMD virus (A/CHA/09 strain) were used as detector antibody. On the nitrocellulose membrane of the immunochromatographic strip,the capture antibody was laid on a sample pad,the detector antibody was printed at the test line(T) and goat anti-guinea pigs IgG antibodies were immobilized to the control line(C). The lower detection limit of the test for a FMDV 146S antigen is 11.7ng/ml as determined in serial tests after the strip device was assembled and the assay condition optimization. No cross reactions were found with FMDV serotype C,Swine vesicular disease (SVD),Vesicular stomatiti svirus (VSV) and vesicular exanthema of swine virus (VES) viral antigens with this rapid test. Clinically,the diagnostic sensitivity of this test for FMDV serotypes A was 88.7% which is as same as an indirect-sandwich ELISA. The specificity of this strip test was 98.2% and is comparable to the 98.7% obtained with indirect-sandwich ELISA. This rapid strip test is simple,easy and fast for clinical testing on field sites; no special instruments and skills are required,and the result can be obtained within 15 min. To our knowledge,this is the first rapid immunochromatogarpic assay for serotype A of FMDV.Tao Jiang Zhong Liang Wei-wei Ren Juan Chen Xiao-ying Zhi Guang-yu Qi Xiang-tao Liu Xue-peng Cai 2011Virologica Sinica2011,26,1:22
13Accelerated Degradation Reliability Modeling and Test Data Statistical Analysis of Aerospace Electrical Connector显示文摘As few or no failures occur during accelerated life test,it is difficult to assess reliability for long-life products with traditional life tests.Reliability assessment using degradation data of product performance over time becomes a significant approach.Aerospace electrical connector is researched in this paper.Through the analysis of failure mechanism,the performance degradation law is obtained and the statistical model for degradation failure is set up;according to the research on statistical analysis methods for degradation data,accelerated life test theory and method for aerospace electrical connector based on performance degradation is proposed by improving time series analysis method,and the storage reliability is assessed for Y11X series of aerospace electrical connector with degradation data from accelerated degradation test.The result obtained is basically consistent with that obtained from accelerated life test based on failure data,and the two estimates of product's characteristic life only have a difference of 8.7%,but the test time shortens about a half.As a result,a systemic approach is proposed for reliability assessment of highly reliable and long-life aerospace product.CHEN Wenhua LIU Juan GAO Liang PAN Jun ZHOU Shengjun 2011Chinese Journal of Mechanical Engineering2011,24,6:22
14Ten cities cross-sectional questionnaire survey of children asthma and other allergies in China显示文摘Asthma,rhinitis and eczema(allergic or non-allergic)have increased throughout the world during the last decades,especially among children.Changes in the indoor environment are suspected to be important causes.China has experienced a dramatic change in indoor environmental exposures during the past two decades.However,such changes and their associations with children’s asthma and other health aspects have not been thoroughly studied.China,Children,Homes,Health(CCHH),Phase I,was a cross-sectional questionnaire survey of 48219 children 1–8 years old in 10 Chinese cities during 2010–2012.The questionnaire includes the International Study of Asthma and Allergies in Childhood(ISAAC)core health questions and additional questions regarding housing,life habits and outdoor environment.In health analyses,children aged 3–6 years old were included.The prevalences of doctor diagnosed asthma varied from 1.7%to 9.8%(mean 6.8%),a large increase from 0.91%in 1999 and 1.50%in2000.The prevalence of wheeze,rhinitis and atopic eczema(last 12 months)varied from 13.9%to 23.7%,24.0%to 50.8%and4.8%to 15.8%,respectively.Taiyuan had the lowest prevalences of all illnesses and Shanghai the highest,except for wheezewhere the highest value was for Urumqi.We found(1)no obvious association between disease prevalences and ambient PM10concentrations and(2)higher prevalences of disease in humid climates with hot summers and cold winters,but with no centrally heated buildings.Associations between the diseases and economic status as indexed by Gross Domestic Product(GDP)requires further study.ZHANG YinPing LI BaiZhan HUANG Chen YANG Xu QIAN Hua DENG QiHong ZHAO ZhuoHui LI AnGui ZHAO JiaNing ZHANG Xin QU Fang HU Yu YANG Qin WANG Juan ZHANG Ming WANG Fang ZHENG XiaoHong LU Chan LIU ZhiJian SUN YueXia MO JinHan ZHAO YiLi LIU Wei WANG TingTing NORBCK Dan BORNEHAG Carl-Gustaf SUNDELL Jan 2013Chinese Science Bulletin2013,58,34:22
15Neuroprotective effects of salidroside on focal cerebral ischemia/reperfusion injury involve the nuclear erythroid 2-related factor 2 pathway显示文摘Salidroside,the main active ingredient extracted from Rhodiola crenulata,has been shown to be neuroprotective in ischemic cerebral injury,but the underlying mechanism for this neuroprotection is poorly understood.In the current study,the neuroprotective effect of salidroside on cerebral ischemia-induced oxidative stress and the role of the nuclear factor erythroid 2-related factor 2(Nrf2)pathway was investigated in a rat model of middle cerebral artery occlusion.Salidroside(30 mg/kg)reduced infarct size,improved neurological function and histological changes,increased activity of superoxide dismutase and glutathione-S-transferase,and reduced malon-dialdehyde levels after cerebral ischemia and reperfusion.Furthermore,salidroside apparently increased Nrf2 and heme oxygenase-1 expression.These results suggest that salidroside exerts its neuroprotective effect against cerebral ischemia through anti-oxidant mechanisms and that activation of the Nrf2 pathway is involved.The Nrf2/antioxidant response element pathway may become a new therapeutic target for the treatment of ischemic stroke.Jing Han Qing Xiao Yan-hua Lin Zhen-zhu Zheng Zhao-dong He Juan Hu Li-dian Chen 2015Neural Regeneration Research2015,10,12:23
16Tau-mediated Neurodegeneration and Potential Implications in Diagnosis and Treatment of Alzheimer's Disease显示文摘Xi-Lin Wu Juan Pina-Crespo Yun-Wu Zhang Xiao-Chun Chen Hua-Xi Xu 2017Chinese Medical Journal2017,,24:22
17The pharmacology, toxicology and therapeutic potential of anthraquinone derivative emodin显示文摘Emodin(1, 3, 8-trihydroxy-6-methylanthraquinone) is a derived anthraquinone compound extracted from roots and barks of pharmaceutical plants, including Rheum palmatum, Aloe vera, Giant knotweed, Polygonum multiflorum and Polygonum cuspidatum. The review aims to provide a scientific summary of emodin in pharmacological activities and toxicity in order to identify the therapeutic potential for its use in human specific organs as a new medicine. Based on the fundamental properties, such as anticancer, anti-inflammatory, antioxidant, antibacterial, antivirs, anti-diabetes, immunosuppressive and osteogenesis promotion, emodin is expected to become an effective preventive and therapeutic drug of cancer, myocardial infarction, atherosclerosis, diabetes, acute pancreatitis, asthma, periodontitis, fatty livers and neurodegenerative diseases. This article intends to provide a novel insight for further development of emodin, hoping to reveal the potential of emodin and necessity of further studies in this field.CUI Ya CHEN Liu-Jing HUANG Tu YING Jian-Qiong LI Juan 2020Chinese Journal of Natural Medicines2020,18,6:23
18Preliminary molecular epidemiology of the Staphylococcus aureus in lower respiratory tract infections: a multicenter study in China显示文摘LI De-zhi CHEN Yu-sheng YANG Jing-ping ZHANG Wei HU Cheng-ping LI Jia-shu MU Lan HU Ying-hui GENG Rong HU Ke CAI Shao-xi WAN Huan-ying WANG Qiu-yue WEI Li-ping DU Juan YU Qin ZHONG Xiao-ning WANG Rui-qin MA Jian-jun TIAN Gui-zhen WANG Si-qin GAO Zhan-cheng 2011Chinese Medical Journal2011,,5:21
19Antitumor immunopreventive and immunotherapeutic effect in mice induced by hybrid vaccine of dendritic cells and hepatocarcinoma in vivo显示文摘AIM: To develop atumor vaccine by fusion of H22 hepatocarcinoma cells and DC, and to study its protective and therapeutical effect against H22 cell.METHODS: H22-DC vaccine was produced by PEG fusion of H22 and DC induced by cytokine released from splenic mononuclear cells, sorted by CD11c magnetic microbead marker. It was injected through the tail vein of the mice and the H22-DC oncogenesis was detected in the liver, spleen and lung. In order to study the therapeutical and protective effect of H22-DC against tumor H22, two groups were divided:immune group and therapeutic group. Immune group was further divided into P, D, HD and H subgroups, immunized by PBS, DC, H22-DC and inactivated H22, respectively, and attacked by H22 cell. The tumor size, tumor weight, mice survival time and tumor latent period were recorded and statistically analyzed; Therapeutical group was divided into three subgroups of P, D and HD, and attacked by H22, then treated with PBS, DC, and H22-DC, respectively. Pathology and flow cytometry were also applied to study the mechanism how the H22-DC vaccine attacked on the H22 cell.RESULTS: 1. No oncogenesis was found in spleen, lung and liver after H22-DC injection. 2. Hybrid vaccine immunized mice had strongest CTL activity. 3. In the immune group,latent period was longer in HD subgroup than that in P, H and D subgroup; and tumor size and weight were smaller in HD subgroup than that in P, H and D subgroup. 4. In therapeutic group, tumor size was smaller in HD subgroup than that in P, D subgroup.CONCLUSION: 1. H22-DC tumor vaccine is safe without oncogenesis in vivo. 2. Hybrid vaccine can stimulate potent specific CTL activity against H22.3. H22-DC vaccine has distinctive prophylatic effect on tumor H22 and can inhibit the tumor growth.Jin-Kun Zhang Jun Li Juan Zhang Hai-Bin Chen Su-Biao Chen Cancer Pathology Laboratory, Shantou University Medical College, Shantou 515031, Guangdong Province, China 2003World Journal of Gastroenterology2003,9,3:21
20The Role of Fibrocyte in the Pathogenesis of Silicosis显示文摘Exposure to free silica induces silicosis and myofibroblasts are regarded as primary effector cells.Fibrocytes can differentiate into myofibroblast.Therefore,the present study was designed to investigate whetherLI Juan YAO Wu HOU Jian Yong ZHANG Lin BAO Lei CHEN Hui Ting WANG Di YUE Zhong Zheng LI Yi Ping ZHANG Miao YU Xing Hao ZHANG Jian Hui QU Ya Qian HAO Chang Fu 2018Biomedical and Environmental Sciences2018,31,4:20
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