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| 1 | Growth inhibition and apoptosis induction Sulindac on Human gastric cancer cells显示文摘AIM: To evaluate the effects of sulindac in inducing growth inhibition and apoptosis of human gastric cancer cells in comparison with human hepatocellular carcinoma (HCC)cells.METHODS: The human gastric cancer cell lines MKN45 and MKN28 and human hepatocellular carcinoma cell lines HepG2and SMMC7721 were used for the study. Anti-proliferative effect was measured by MTT assay, and apoptosis was determined by Hoechst-33258 staining, electronography and DNA fragmentation. The protein of cyclooxygenase-2 (COX(2) and Bcl-2 were detected by Westem dot blotting.RESULTS: Sulindac could initiate growth inhibition and apoptosis of MKN45, MKN28, HepG2 and SMMC7721 cells in a dose-and time-dependent manner. Growth inhibitory activity and apoptosis were more sensitive in HepG2 cells than in SMMC7721 cells, MKN45 and MKN28 cells. After 24hours incubation with sulindac at 2mmol. L-1 and 4mmol.L-1, the level of COX-2 and Bcl-2 protein were lowered in MKN45, SMMC7721 and HepG2 cells but not in MKN28 cells.CONCLUSION: Sulindac could inhibit the growth of gastric cancer cells and HCC cells effectively in vitro by apoptosis induction, which was associated with regression of COX-2and Bcl-2 expression. The growth inhibition and apoptosis of HCC cells were greater then that of human gastric cancer cells. The different effects of apoptosis in gastric cancer cells may be related to the differentiation of the cells. | Yun-Lin Wu~1 Bo Sun~1 Xue-Jun Zhang~2 Sheng-Nian Wang~2 Heng-Yi He~2 Min-Min Qiao~1 Jie Zhong~1 Jia-Yu Xu~1 1 Department of Gastroenterology,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China2 Institude of Biochemistry and Cell Biology,Shanghai Institues for Biological Sciences,Chinese Academy of Sciences.Shanghai 200025,China | 2001 | World Journal of Gastroenterology2001，7，6： | 64 |
| 2 | A meta-analysis of oxidative stress markers in schizophrenia显示文摘Oxidative stress has been identified as a possible element in the neuropathological processes of schizophrenia(SCZ).Alteration of oxidative stress markers has been reported in SCZ studies,but with inconsistent results.To evaluate the risk of oxidative stress to schizophrenia,a meta-analysis was conducted,including five markers of oxidative stress [thiobarbituric reactive substances(TBARS),nitric oxide(NO),catalase(CAT),glutathione peroxidase(GP) and superoxide dismutase(SOD)] in SCZ patients versus healthy controls.This study showed that TBARS and NO significantly increased in SCZ,while SOD activity significantly decreased in the disorganized type of SCZ patients.No significant effect size was found for the activities of GP and CAT in SCZ patients(P>0.05).Egger’s regression test observed no significant publication bias across the oxidative stress markers,but found high heterogeneities in all the 5 markers.The subgroup analysis suggested that the ethnicity,sample size of patients and sample sources may contribute to the heterogeneity of the results for TBARS,NO and SOD.The result further demonstrated the involvement of oxidative stress in the pathophysiology of schizophrenia. | ZHANG Ming1,2,ZHAO ZhongMing3,HE Lin1,2,4 & WAN ChunLing1,2 1 Bio-X Center,Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders(Ministry of Education),Shanghai Jiao Tong University,Shanghai 200030,China 2 Institutes for Nutritional Sciences,Shanghai Institute of Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China 3 Departments of Biomedical Informatics,Psychiatry,and Cancer Biology,Vanderbilt University Medical Center,Nashville,TN 37232,USA 4 Institutes of Biomedical Sciences,Fudan University,Shanghai 200032,China | 2010 | Science China(Life Sciences)2010，53，1： | 32 |
| 3 | Above-and belowground biomass in relation to envi- ronmental factors in temperate grasslands, Inner Mongolia显示文摘Above- and belowground biomasses of grasslands are important parameters for characterizing re- gional and global carbon cycles in grassland ecosystems. Compared with the relatively detailed in- formation for aboveground biomass (AGB), belowground biomass (BGB) is poorly reported at the re- gional scales. The present study, based on a total of 113 sampling sites in temperate grassland of the Inner Mongolia, investigated regional distribution patterns of AGB, BGB, vertical distribution of roots, and their relationships with environmental factors. AGB and BGB increased from the southwest to the northeast of the study region. The largest biomass occurred in meadow steppe, with mean AGB and BGB of 196.7 and 1385.2 g/m2, respectively; while the lowest biomass occurred in desert steppe, with an AGB of 56.6 g/m2 and a BGB of 301.0 g/m2. In addition, about 47% of root biomass was distributed in the top 10 cm soil. Further statistical analysis indicated that precipitation was the primary determinant factor in shaping these distribution patterns. Vertical distribution of roots was significantly affected by precipitation, while the effects of soil texture and grassland types were weak. | MA WenHong1,2, YANG YuanHe1, HE JinSheng1, ZENG Hui1,2 & FANG JingYun1 1 Department of Ecology, College of Urban and Environmental Sciences, Peking University, Beijing 100871, China 2 School of Environment & Urban Study, Peking University Shenzhen Graduate School, Shenzhen 518055, China | 2008 | Science China(Life Sciences)2008，51，3： | 34 |
| 4 | The prognostic value of preoperative serum levels of CEA,CA19-9 and CA72-4 in patients with colorectal cancer显示文摘INTRODUCTIONCarcinoembryonic antigen ( CEA) , originally described by Gold and Freedman [1] in 1965, is now an acknowledged member of immunoglobulin superfamily[2],with a role as an intracellular adhesion molecule[3].Carbohydrate antigen 19-9(CA19-9), obtained with a monoclonal antibody produced by immunizing a monoclonal antibody produced by immunizing a mouse with a colonic cancer cell line in 1979[4],is a ligand for E-selectin that plays an important role in the addhesion of cancer cells to endothelial cells [5,6]. | Chao Xu Zheng~1 Wen Hua Zhan~1 Ji Zong Zhao~2 Dong Zheng~3 Dong Ping Wang~1 Yu Long He~1 Zhang Qing Zheng~1 ~1Department of General Surgery,~2Laboratory of Surgery,~3Department of Medicine,First Affiliated Hospital,Sun Yat-Sen University of Medical Sciences,Guangzhou 510080,Guangdong Province,ChinaDr.Chao Xu Zheng now working as a surgeon and lecturer in the Department of General Surgery,First Affiliated Hospital,Sun Yat-Sen University of Medical Sciences,who is a Ph.D.student,having 7 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 25 |
| 5 | Landscape ecological assessment and eco-tourism development in the South Dongting Lake Wetland, China显示文摘As an important resource and the living environment of mankind, wetland has become gradually a highlight, strongly concerned and intensively studied by scientists and sociologists. The governments in the world and the whole society have been paying more and more attention on it. The Dongting Lake of China is regarded as an internationally important wetland. For a rational development and protection of the wetland, an investigation and studied on its resources and its value to tourism in the South Dongting Lake was conducted, to create an assessment system of the ecological landscapes, and to evaluate qualitatively and quantitatively the value of wetland landscape to the eco-tourism. The results showed that the scenic value of the South Dongting Lake Wetland satisfied the criterion of AAAA grade of China national scenic attraction. The eco-tourism value of the landscape cultures in the South Dongting Lake Wetland was discussed with emphasis. It were formulated that a principle and frame of sustainable exploitation of the wetland landscapes and it was proposed as well that establishing a Wetland Park and developing eco-tourism in the South Dongting Lake. Wetland is a fragile ecosystem with low resistance to the impact of the exploitation. Thus, we must pay intensively attention to the influence of exploitation on the landscape, take the ecological risk in account to employ a right countermeasure and avoid the negative affection. | HE Ping 1,2, WANG Bao-zhong 1,3 (1.Central South Forestry University, Zhuzhou 412006, China 2.China International Engineering Consulting Corporation, Beijing 100044, China 3.Nanjing University, Nanjing 210093, China | 2003 | Journal of Environmental Sciences2003，15，2： | 19 |
| 6 | EFFECT OF COPPER AND BORON CONTENT ON STRAIN-INDUCED Nb(C,N) PRECIPITATION IN ULCB STEELS AT HOT DEFORMATION TEMPERATURE显示文摘The stress relaxation curves of Ultra-Low Carbon Bainitic(ULCB) steels with different Cu and B contents were measured by using Gleeble-1500 dynamic thermal-mechanical simulator. The results show that Cu and B added can accelerate the strain-induced precipitation reaction, and the effect of Cu and B is even more obvious with Cu and B combined addition or Cu content increased. The TEM analysis of precipitate engendered at the temperature of 850℃ C indicate that Nb(C,N) precipitate nucleates dominantly on the dislocation line, and grows with holding time extended while the precipitate particle size increases from 5 nm to 17 nm. | G.F. Zhou, M. B. Wen, P. H. Li and X. L. He 1) National Enterprise Technology Center, Wuhan Iron & Steel Group Co., Wuhan 430080, China 2) University of Science and Technology Beijing, Beijing 100083, China | 2000 | Acta Metallurgica Sinica(English Letters)2000，13，2： | 16 |
| 7 | Clinical significance of Fas antigen expression in gastric carcinoma显示文摘INTRODUCTIONInordertodeterminewhetherFasantigenplaysaroleinthegastriccarcinogenicsequence,animmunohistochemicalstudyofFasant... | LIU Hai Feng 1, LIU Wei Wen 1, FANG Dian Chun 1, LIU Feng Xian 2 and HE Guang You 2 | 1999 | World Journal of Gastroenterology1999，5，1： | 15 |
| 8 | Complete nucleotide sequence of a novel porcine circovirus-like agent and its infectivity in vitro显示文摘A novel agent (hence termed as P2) was isolated from pig sera in China, which contained covalently bound circular genomic DNAs of 993 nucleotides. Sequence analyses indicated that the agent was closely related to the porcine circovirus (PCV). The molecular clone of P2 was constructed subsequently and used for the following studies. Intracytoplasmic inclusions and intranuclear inclusions were only found in PK-15 cells transfected with the tandem dimer of P2 molecular DNA clone. Intracytoplasmic inclusions were round or irregular in shape and 0.1-0.4 μm in diameter, and intranuclear inclusions were electronically denser than intracytoplasmic inclusions and had two general shapes: round/small (0.1 μm in diameter) and hexagonal/large (0.5―1.4 μm in diameter). The inclusions were not membranously bound. The cells transfected with the tandem dimer of P2 molecular DNA clone were tested positive for P2 DNA at passages 5. The P2 antigen could be detected in both transfected and passaged PK-15 cells. This is the first report regarding the complete nucleotide sequence of a small DNA genome in a circovirus-like infectious agent in vitro. | WEN LiBin1, HE KongWang1, YANG HanChun2, NI YanXiu1, ZHANG XueHan1, GUO RongLi1 & PAN QunXin1 1 Key Laboratory of Animal and Poultry Diseases Diagnostic of the Ministry of Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China 2 Key Laboratory of Preventive Veterinary Medicine of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China | 2008 | Science China(Life Sciences)2008，51，5： | 14 |
| 9 | Orbit determination for geostationary satellites with the combination of transfer ranging and pseudorange data显示文摘Geostationary satellites(GEOs) play a significant role in the regional satellite navigation system.Simulation experiments show that the clock corrections could be mitigated through a single strategy or double differencing strategies for a navigation constellation,but for the mode of individual GEO orbit determination,high precision orbit and clock correction could not be obtained in the orbit determination based on the pseudorange data.A new GEO combined precise orbit determination(POD) strategy is studied in this paper,which combines pseudorange data and C-band transfer ranging data.This strategy overcomes the deficiency of C-band transfer ranging caused by limited stations and tracking time available.With the combination of transfer ranging and pseudorange data,clock corrections between the GEO and the stations can be estimated simultaneously along with orbital parameters,maintaining self-consistency between the satellite ephemeris and clock correction parameters.The error covariance analysis is conducted to illuminate the contributions from the transfer ranging data and the psudoranging data.Using data collected for a Chinese GEO satellite with 3 C-band transfer ranging stations and 4 L-band pseudorange tracking stations,POD experiments indicate that a meter-level accuracy is achievable.The root-mean-square(RMS) of the post-fit C-band ranging data is about 0.203 m,and the RMS of the post-fit pseudorange is 0.408 m.Radial component errors of the POD experiments are independently evaluated with the satellite laser ranging(SLR) data from a station in Beijing,with the residual RMS of 0.076 m.The SLR evaluation also suggests that for 2-h orbital predication,the predicted radial error is about 0.404 m,and the clock correction error is about 1.38 ns.Even for the combination of one C-band transfer ranging station and 4 pseudorange stations,POD is able to achieve a reasonable accuracy with the radial error of 0.280 m and the 2-h predicted radial error of 0.888 m.Clock synchronization between the GEO and tracking stations is achieved with an estimated accuracy of about 1.55 ns,meeting the navigation service requirements. | GUO Rui1,2,3,4,HU XiaoGong2,4,LIU Li1,WU XiaoLi1,HUANG Yong2,4 & HE Feng1 1 Beijing Global Information Application and Development Center,Beijing 100094,China 2 Shanghai Astronomical Observatory,Chinese Academy of Sciences,Shanghai 200030,China 3 Graduate University of Chinese Academy of Sciences,Beijing 100049,China 4 Space Navigation and Positioning Technology,Laboratory of Shanghai Municipal,Shanghai 200030,China | 2010 | Science China(Physics,Mechanics & Astronomy)2010，53，9： | 13 |
| 10 | RESEARCH ON THE EVALUATION METHOD OF FRICTION AND LUBRICATION IN DEEP DRAWING显示文摘The deformation characteristic of bland in deep drawing is discussed. It is pointed out that the friction and lubrication conditions in for drawing are different from that in mechanical motion or machine work or other plastic process. The common test methods in laboratories are analyzed. It shows that though all those test methods can test the friction coefficient, the probe test method is most suitable for the research of friction and lubrication and the process in deep drawing, for this method is identical with the actual work condition either from the test principle or deformation status of the blank. Last the successful application in the deep drawing simulator newly developed the the probe method are intro- duced in detail. | D. N. He, X. F. Yin, H. Z. Thao, D. L*, X. Y. Ruan, J. L. Cheng and J. Y. Jiang 1) Shanghai Jiao Tong University, National Die & Mold CAD E. R. C, Shanghai 200030, China 2) Shanghai Vopkswagen Automotive Company Ltd., Shanghai 201805, China Manuscript re | 2000 | Acta Metallurgica Sinica(English Letters)2000，13，2： | 13 |
| 11 | Geochronological evidence of Indosinian(high-pressure) metamorphic event and its tectonic significance in Taxkorgan area of the Western Kunlun Mountains,NW China显示文摘The CL images,LA-ICP-MS in situ trace elements analysis,and U-Pb dating for zircons indicate that the metamorphic ages of the sillimanite-garnet-biotite gneiss and the garnet-amphibole gneiss from eastern Taxkorgan of the Western Kunlun Mountains are 220±2 and 220±3 Ma respectively,and their protolith ages are younger than 253±2 and 480±8 Ma respectively.Two samples were collected at the same outcrops with HP mafic granulite and HP pelitic granulite.Mineral assemblage of the sillimanite-garnet-biotite gneiss(Grt+Sill+Per+Q) is consistent with that of HP pelitic granulite at early high amphibolite-granulite facies stage.Mineral assemblage of the garnet-amphibole gneiss(Grt+Amp+Pl+Q) is consistent with retro-metamorphic assemblage of HP mafic granulite at amphibolite facies stage.The dating results suggest that these HP granulites underwent peak metamorphism at 220±2 to 253±2 Ma.Thus,the Kangxiwar tectonic zone was probably formed by subduction and collision of the Paleo-Tethys Ocean during Indosinian.Protolith ages of the two samples,together with previously published U-Pb zircon dating age,suggest that the sillimanite-garnet schist-quartzite unit is a late Paleozoic unit,not a part of the Paleoproterozoic Bulunkuole Group. | YANG WenQiang1,LIU Liang1,CAO YuTing1,WANG Chao1,2,HE ShiPing2,LI RongShe2 & ZHU XiaoHui1 1 State Key Laboratory of Continental Dynamics,Department of Geology,Northwest University,Xi’an 710069,China 2 Xi’an Institute of Geology and Mineral Resource,Xi’an 710054,China | 2010 | Science China Earth Sciences2010，53，10： | 13 |
| 12 | Thermal decomposition kinetics of titanium hydride and Al alloy melt foaming process显示文摘A temperature programmed decomposition (TPD) apparatus with metal tube struc- ture, in which Ar is used as the carrier gas, is established and the TPD spectrum of titanium hy- dride is acquired. Using consulting table method (CTM), spectrum superposition method (SSM) and differential spectrum technique, TPD spectrum of titanium hydride is separated and a set of thermal decomposition kinetics equations are acquired. According to these equations, the rela- tionship between decomposition quantity and time for titanium hydride at the temperature of 940 K is obtained and the result well coincides with the Al alloy melt foaming process. | YANG Donghui*1, HE Deping*1& YANG Shangrun*2 1. Department of Materials Science and Engineering, Southeast University, Nanjing 210096, China 2. Department of Chemistry, Nanjing University, Nanjing 210093, China | 2004 | Science China Chemistry2004，47，6： | 12 |
| 13 | A LOOP DRAWBEAD RESTRAINING FORCE MODEL IN SHEET METAL DEEP DRAWING PROCESS显示文摘In sheet metal forming, drawbeads are often used to control uneven material flow which may cause defects such as wrinkles, fracture, surface distortion and springback. Appropriate setting and adjusting the drawbead force is one of the most important pa- rameters in sheet metal forming process control. This paper derives the model of loop drawbead restraining force (DBRF) using plastic forming theory, and gives the min imum blankholding force. Experiments are carried out to verify the proposed DBRF model. The good agreement between the calculated values and experiments data justi- fies the proposed loop drawbead restraining force model. | Y.J. Luo, S.P. Li, J.F. Zheng, D.N. He, Y.Q. Zhang, J.L. Cheng and J. Y. Jiang 1)National Mold & Die CAD Engineering Research Center, Shanghai 200030, China 2)Shanghai Volkswagen Automotive Company Ltd.(SVM), Shanghai 201805, China | 2001 | Acta Metallurgica Sinica(English Letters)2001，14，5： | 11 |
| 14 | High frequencies of HGV and TTV infections in blood donors in Hangzhou显示文摘ALM To determine the frequencies of HGV and TTVinfections in blood donors in Hangzhou.METHODS RT-nested PCR for HGV RNA detection and semi-nested PCR for TTV DNA detection in the sera from 203 blood donors, and nucleotide sequence analysis were performed.``RESULTS Thirty-two ( 15.8%) and 30 (14.8%) of the 203serum samples were positive for HGV RNA and TTV DNA,respectively. And 5 (2.5%) of the 203 serum samples were detectable for both HGV RNA and TTV DNA.Homology of the nucleotide sequences of HGV RT-nested PCR products and TTV semi-nested PCR products from 3serum samples compared with the reported HGV and TTV sequences was 89.36%. 87.94%, 88.65% and 63.51%.65.77% and 67.12%. respectively.``CONCLUSION The infection rates of HGV and/or TTV inblood donors are relatively high. and to establish HGV and TTV examinations to screen blood donors is needed for transfusion security. The genomic heterogeneity of TTV or HGV is present in the isolates from different areas. | Jie Yan~1 Li-Li Chen~2 Yi-Hui Luo~1 Ya-Fei Mao~1 Meng He~1 1 Department of Pathogenic Biology,Medical School of Zhejiang University,Hangzhou 310006,Zhejiang Province,China2 Department of Stomatology,The Second Affiliated Hospital,Medical School of Zhejiang University,Hangzhou 310009,Zhejiang Province,China | 2001 | World Journal of Gastroenterology2001，7，5： | 11 |
| 15 | Proteomics in China:Ready for prime time显示文摘Proteomics is a newborn science focusing on the comprehensive systematic analysis of all proteins in molecule machineries,organelles,cells,tissues,organs or intact organisms.It has been becoming one of the focuses in life sciences and cutting-edge techniques in biotechnologies in the 21st century.During the last decade,proteomics in China has developed much faster than other developing fields in the life sciences.This review article briefly retrospects the origin and development of proteomics in China,and provides an overview of representative scientific progress and perspectives. | GAO Xue1,ZHANG XueLi1,ZHENG JunJie1 & HE FuChu1,2 1 State Key Laboratory of Proteomics,Beijing Proteome Research Center,Beijing Institute of Radiation Medicine,Beijing 102206,China 2 Institutes of Biomedical Sciences,Fudan University,Shanghai 200032,China | 2010 | Science China(Life Sciences)2010，53，1： | 10 |
| 16 | Discovery and identification of Serum Amyloid A protein elevated in lung cancer serum显示文摘Two hundred and eighteen serum samples from 175 lung cancer patients and 43 healthy individuals were analyzed by using Surface Enhaced Laser Desorption/Ionization Time of Flight Mass Spectrome- try (SELDI-TOF-MS). The data analyzed by both Biomarker Wizard? and Biomarker Patterns? software showed that a protein peak with the molecular weight of 11.6 kDa significantly increased in lung cancer. Meanwhile,the level of this biomarker was progressively increased with the clinical stages of lung cancer. The candidate biomarker was then obtained from tricine one-dimensional sodium dodecyl sul- fate-polyacrylamide gel electrophoresis by matching the molecular weight with peaks on WCX2 chips and was identified as Serum Amyloid A protein (SAA) by MALDI/MS-MS and database searching. It was further validated in the same serum samples by immunoprecipitation with commercial SAA antibody. To confirm the SAA differential expression in lung cancer patients, the same set of serum samples was measured by ELISA assay. The result showed that at the cutoff point 0.446(OD value)on the Receiver Operating Characteristic (ROC) curve, SAA could better discriminate lung cancer from healthy indi- viduals with sensitivity of 84.1% and specificity of 80%. These findings demonstrated that SAA could be characterized as a biomarker related to pathological stages of lung cancer. | DAI SongWei1,2, WANG XiaoMin1,2, LIU LiYun1,2, LIU JiFu3, WU ShanShan3, HUANG LingYun1,2, XIAO XueYuan1,2 & HE DaCheng1,2 1 Key Laboratory of Cell Proliferation and Regulation of Ministry of Education, Beijing Normal University, Beijing 100875, China 2 Universities’ Confederated Institute of Proteomics, Beijing 100875, China 3 Department of Thoracic Surgery, General Hospital of Beijing Unit, PLA, Beijing 100500, China | 2007 | Science China(Life Sciences)2007，50，3： | 10 |
| 17 | Synchronous estimation of DTM and fractional vegetation cover in forested area from airborne LIDAR height and intensity data显示文摘We proposed a method to separate ground points and vegetation points from discrete return,small footprint airborne laser scanner data,called skewness change algorithm.The method,which makes use of intensity of laser scanner data,is especially applicable in steep,and forested areas.It does not take slope of forested area into account,while other algorithms consider the change of slope in steep forested area.The ground points and vegetation points can be used to estimate digital terrain model(DTM) and fractional vegetation cover,respectively.A few vegetation points which were classified into the ground points were removed as noise before the generation of DTM.This method was tested in a test area of 10000 square meters.A LiteMapper -5600 laser system was used and a flight was carried out over a ground of 700―800 m.In this tested area,a total number of 1546 field measurement ground points were measured with a total station TOPCON GTS-602 and TOPCON GTS -7002 for validation of DTM and the mean error value is -18.5 cm and the RMSE(root mean square error) is ±20.9 cm.A data trap sizes of 4m in diameter from airborne laser scanner data was selected to compute vegetation fraction cover.Validation of fractional vegetation cover was carried out using 15 hemispherical photographs,which are georeferenced to centimeter accuracy by differential GPS.The gap fraction was computed over a range of zenith angles 10° using the gap light analyzer(GLA) from each hemispherical photograph.The R2 for the regression of fractional vegetation cover from these ALS data and the respective field measurements is 0.7554.So this study presents a method for synchronous estimation of DTM and fractional vegetation cover in forested area from airborne LIDAR height and intensity data. | BAO YunFei1,2,CAO ChunXiang1,ZHANG Hao1,CHEN ErXue3,HE QiSheng1,2,HUANG HuaBing1,LI ZengYuan3,LI XiaoWen1,4 & GONG Peng1 1 State Key Laboratory of Remote Sensing Science,jointly sponsored by the Institute for Remote Sensing Applications of Chinese Academy of Sciences and Beijing Normal University,Beijing 100101,China 2 Graduate University of the Chinese Academy of Sciences,Beijing 100049,China 3 Institute of Forest Resource Information Technique,the Chinese Academy of Forestry,Beijing 100091,China 4 Research Center for Remote Sensing and GIS,Department of Geography and Beijing Key Laboratory for Remote Sensing of Environment and Digital Sites,Beijing Normal University,Beijing 100875,China | 2008 | Science China(Technological Sciences)2008，51，S2： | 10 |
| 18 | Senescence-like changes induced by expression of p21^(Waf1/Cip1) in NIH3T3 cell line显示文摘P21Waf1/Cip1 is a potent cyclin-dependent kinase inhibitor. As a downstream mediator of p53, p21Waf1/cip1involves in cell cycle arrest, differentiation and apoptosis. Previous studies in human cells provided evidencefor a link between p21Waf1/cip1 and cellular senescence. While in murine cells, the role of p21Waf1/Cip1is indefinite. We explored this issue using NIH3T3 cells with inducible p21Waf1/cip1 expression. Induc-tion of p21Waf1/Cip1 triggered G1 growth arrest, and NIH3T3-p21 cells exhibited morphologic features,such as enlarged and flattened cellular shape, specific to the senescence phenotype. We also showed thatp21Waf1/Cip1-transduced NIH3T3 cells expressedβ-galactosidase activity at pH 6.0, which is known to bea marker of senescence. Our results suggest that p21Waf1/cipx can also induce senescence-like changes inmurine cells. | XI CHEN1, WEI ZHANG2, YUN FEI GAO1, XIAO QIN SU1, ZHONG HE ZHAI1,*1 College of Life Sciences, Peking University, Beijing 100871, China2 The Key Laboratory of Cell Proliferation and Regulation Biology of the Ministry of Education, College of Life Sciences, | 2002 | Cell Research2002，12，4： | 9 |
| 19 | Rainfall interception by sand-stabilizing shrubs related to crown structure显示文摘On the edge of the Tengger Desert in northern China,revegetation has changed the landscape from moving dunes to stabilized dunes covered by shrubs,which further modifies the pattern of rainfall redistribution.To study rainfall interception loss by shrubs and its relationship to rainfall properties and crown structure,throughfalls passing through crowns of Artemisia ordosica Krash.and Caragana korshinskii Kom.were measured using nine PVC cups under the canopy of each of the two shrubs during 73 rain events over a three-year period,with total rainfall of 260.9 mm.Interception losses of gross rainfall by A.ordosica and C.korshinskii account for 15% and 27% of the total on a crown area basis,and 6% and 11% on a ground area basis,respectively.Individual throughfall(T) and interception(I) were significantly related to rainfall amount(Pg),duration(D),and intensity(R).Ratios of throughfall to rainfall(T/Pg) and interception to rainfall(I/Pg) were not only significantly related to Pg,D,and R,but also to shrub species,and interactions of species with crown volume(CV) and leaf area index(LAI).Under most rain events,interceptions by C.korshinskii with greater CV and LAI were significantly higher than those by A.ordosica,and more rainfall interception occurred at locations closer to the stems of the two shrubs.For C.korshinskii,I/Pg had a significant positive linear relation with CV and LAI,while T/Pg had a significant negative linear relation with them.CV has a greater influence on T/Pg and I/Pg than does LAI.Using a regression method,canopy water storage capacities are estimated to be 0.52 and 0.68 mm,and free throughfall coefficient to be 0.62 and 0.47 for A.ordosica and C.korshinskii,respectively. | ZhiShan Zhang 1*,XinRong Li 1,XueJun Dong 2,XiaoHong Jia 1,MingZhu He 1,HuiJuan Tan 1 1.Shapotou Desert Research and Experiment Station,Cold and Arid Regions Environmental and Engineering Research Institute,Chinese Academy of Sciences,Lanzhou,Gansu 730000,China.2.Central Grasslands Research Extension Center,North Dakota State University,Streeter,ND 58483,USA. | 2009 | Research in Cold and Arid Regions2009，1，2： | 9 |
| 20 | The Inhibitory Effect of Quercetin on IL-6 Production by LPS-Stimulated Neutrophils显示文摘Quercetin is a herbal flavonoid derived from various foods of plant origin and plays a role in anti-inflammation. Although a number of researches in the field have been done, the mechanism of anti-inflammatory effect of quercetin should be further clarified. In the present study, we investigated the effects of quercetin on IL-6 production by LPS-stimulated neutrophils in human. Neutrophils were were pre-treated with quercetin at the final concentrations of ranging from 0-80 μM for 30 min, or not treated, and then incubated in the presence or absence of lipopolysaccharide (LPS) at a final concentration of 100 ng/ml for indicated time. The secretion level of IL-6 in the culture supernatants was assayed by ELISA, the intracellular level of IL-6 was detected by flow cytometry and the expression of IL-6 mRNA was analyzed by RT-PCR. The experiment results showed that neutrophils cultured with medium or quercetin alone did not express IL-6, but LPS (100 ng/ml) induced IL-6 expression of neutrophils. However, after pre-treatment of neutrophils with quercetin (40 μM) for 30 min, the inducible effects of LPS on the increase of IL-6 secretion, intracellular IL-6 level and IL-6 mRNA expression by neutrophils were abrogated. IL-6 is one of the important pro-inflammatory factors, especially in early phage of inflammation. Thus, our data suggested that quercetin might exert its anti-inflammatory effect through negatively modulating pro-inflammatory factors, such as IL-6. The inhibitory effects of quercetin on IL-6 production by neutrophils may provide a theoretical basis on future therapy of inflammation. | Jiajia Liu~(1,2) Xin Li~1 Yang Yue~1 Juan Li~1 Tao He~1 Yanzheng He~1 ~1Laboratory of Molecular Biology,Luzhou Medical College,Luzhou 646000,Sichuan,China ~2Laboratory of Molecular Biology,Luzhou Medical College,Luzhou 646000,Sichuan,China. | 2005 | Cellular & Molecular Immunology2005，2，6： | 9 |
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