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| 1 | Full-thickness tissue engineered skin constructed with autogenic bone marrow mesenchymal stem cells显示文摘To explore the feasibility of repairing clinical cutaneous deficiency, autogenic bone marrow mesen-chymal stem cells (BMSCs) were isolated and differentiated into epidermal cells and fibroblasts in vitro supplemented with different inducing factors and biomaterials to construct functional tissue- engineered skin. The results showed that after 72 h induction, BMSCs displayed morphologic changes such as typical epidermal cell arrangement, from spindle shape to round or oval; tonofibrils, melano-somes and keratohyaline granules were observed under a transmission electronic microscope. The differentiated cells expressed epidermal stem cell surface marker CK19 (59.66% ± 4.2%) and epidermal cells differentiation marker CK10. In addition, the induced epidermal cells acquired the anti-radiation capacity featured by lowered apoptosis following exposure to UVB. On the other hand, the collagen microfibrils deposition was noticed under a transmission electronic microscope after differentiating into dermis fibroblasts; RT-PCR identified collagen type I mRNA expression in differentiated cells; radioimmunoassay detected the secretion of interleukin-6 (IL-6) and interleukin-8 (IL-8) (up to 115.06 pg/mL and 0.84 ng/mL, respectively). Further in vivo implanting BMSCs with scaffold material short-ened skin wound repair significantly. In one word, autogenic BMSCs have the potential to differentiate into epidermal cells and fibroblasts in vitro, and show clinical feasibility acting as epidermis-like and dermis-like seed cells in skin engineering. | HE LiJuan, NAN Xue, WANG YunFang, GUAN LiDong, BAI CiXian, SHI ShuangShuang, YUAN HongFeng, CHEN Lin, LIU DaQing & PEI XueTao Laboratory of Stem Cells and Regenerative Medicine, Institute of Blood Transfusion, Academy of Military Medical Sciences, Beijing 100850, China | 2007 | Science China(Life Sciences)2007，50，4： | 16 |
| 2 | In vitro differentiation of human adipose-derived mesenchymal stem cells into endothelial-like cells显示文摘The neovascularization of ischemic tis- sue is a crucial initial step for the functional rehabili- tation and wound healing. However, the short of seed cell candidate for the foundation of vascular network is still a big issue. Human adipose tissue derived mesenchymal stem cells (hADSCs), which possess multilineage potential, are capable of adipogenic, osteogenic, and chondrogenic differentiation. We examined whether this kind of stem cells could dif- ferentiate into endothelial-like cells and participate in blood vessel formation, and whether they could be used as an ideal cell source for therapeutic angio- genesis in ischemic diseases or vascularization of tissue constructs. The results showed that hADSCs, grown under appropriately induced conditions, dis- played characteristics similar to those of vessel en- dothelium. The differentiated cells expressed endo- thelial cell markers CD34 and vWF, and had high metabolism of acetylated low-density lipoprotein and prostacyclin. In addition, the induced cells were able to form tube-like structures when cultured on matrigel. Our data indicated that induced hADSCs could ex- hibit characteristics of endothelial cells. Therefore, these cells, as a source of human endothelial cells, may find many applications in such realms as engi- neering blood vessels, endothelial cell transplantation for myocardial regeneration, and induction of angio- genesis for treatment of regional ischemia. | GUAN Lidong LI Shaoqing WANG Yunfang YUE Huimin LIU Daqing HE Lijuan BAI Cixian YAN Fang NAN Xue SHI Shuangshuang PEI Xuetao | 2006 | Chinese Science Bulletin2006，51，15： | 6 |
| 3 | 学术论文中问题与方法识别及其关系抽取研究综述显示文摘[目的/意义]问题和方法是学术论文的重要组成部分.将散落在学术论文中的问题与方法进行有效组织,例如问题与方法识别及其之间的关系抽取,可挖掘学术论文中的隐性知识,促进学科的方法体系和问题体系构建.对学术论文中问题与方法识别及其关系抽取的相关研究工作的梳理,有助于把握该研究的发展趋势、发现该研究中存在的不足,并为未来的工作提供借鉴和指导.[方法/过程]在学术论文的问题和方法的挖掘方面,现有研究围绕4个研究点展开,分别是问题与方法及其关系定义、问题与方法及其关系标注数据集构建、问题与方法识别及其关系抽取的模型设计以及问题与方法及其关系的应用.本文分别对这4个研究点进行梳理,归纳总结现有学术论文中问题和方法知识挖掘的现状.[结果/结论]分析发现,在问题与方法的相关定义中,较少结合科学哲学中的问题学等理论进行定义;在问题与方法数据集构建中,存在数据集重复标注的现象,另外,开源数据集集中在自然科学领域且一般为英语语料,中文开源语料稀缺;在问题与方法识别及其关系抽取中,现有抽取模型性能较低;有关问题和方法的研究不应止步于词语识别和关系抽取,需对挖掘出的知识进行深入分析和应用. | 张颖怡 章成志 DAQING HE | 2022 | 图书情报工作2022，66，12： | 5 |
| 4 | Real-time and generic queue time estimation based on mobile crowdsensing显示文摘 | Jiangtao WANG Yasha WANG Daqing ZHANG Leye WANG Chao CHEN Jae Woong LEE Yuanduo HE | 2017 | Frontiers of Computer Science2017，11，1： | 4 |
| 5 | PREPARATION,IDENTIFICATION AND INTERFACIAL ACTIVITIES OF PETROLEUM CARBOXYLATE AS SURFACTANT FOR CHEMICAL FLOODING显示文摘Petroleum carboxylate that may be suitable for tertiary oil recovery have been producedinexpensively from the fractions of Daqing crude oil by a two step process.The feed stock was firstoxidized in the vapor phase,followed by reaction of the oxidized products with sodiumhydroxide.Dilute solutions of sodium carboxylates were produced and showultralow(10-2mN/m)interfacial tensions(IFTs)against a variety of hydrocarbons with a widerange of ACN(Alkane Carbon Number).Infrared spectroscopy was used to identify the functionalgroups in the oxidized and the saponified products. | Hong De HUANG Jianghan Petroleum Institute,Jiangling,Hubei,434102You Zhen YANG Ji Xin TANG Cheng Quan HE Rong Ming ZHANG Song Mei ZHANG Daqing Petroleum Institute,Anda,Heilongjiang,151400 | 1993 | Chinese Chemical Letters1993，4，4： | 3 |
| 6 | NONLINEAR EFFECT OF THE DUALFREQUENCY IP SPECTRUM显示文摘NONLINEAREFFECTOFTHEDUALFREQUENCYIPSPECTRUMHeJishan;LiDaqing;TangJingtian(DepartmentofGeology,CentralSouthUniversityofTechnol... | He Jishan Li Daqing Tang Jingtian(Department of Geology,Central South University of Technology,Changsha 410083) | 1995 | 中国有色金属学会会刊:英文版1995，5，4： | 2 |
| 7 | Ultrasound guided neural stem cell transplantation through the lateral ventricle for treatment of cerebral palsy in children显示文摘A total of 24 children with cerebral palsy were enrolled in this study and underwent ultrasound guided transplantation of neural stem cells through the lateral ventricle. Neural stem cells (3.8 × 106-7.3 × 107) were injected into the lateral ventricles. Mild injury of lateral ventricular blood vessels occurred in only two cases (8.3%). Seven cases (29.2%) experienced a fever. Clinical manifestations were improved to varying degrees in eight cases (28.0%) within 3 months after transplantation. Patient condition did not worsen, and no patient experienced severe adverse reactions. | Sheng He Zuo Luan Suqing Qu Xuan Qiu Daqing Xin Wenkai Jia Yanhua Shen Zehui Yu Tao Xu | 2012 | Neural Regeneration Research2012，7，32： | 2 |
| 8 | Geo-location for Ground Target with Multiple Observations Using Unmanned Aerial Vehicle显示文摘In order to improve the target location accuracy of unmanned aerial vehicle(UAV),a novel target location method using multiple observations is proposed.Firstly,the camera intrinsic parameters are calibrated.Then,the weighted least squares estimation is used to improve the localization precision because the traditional crossover method is vulnerable to noise and has low precision.By repeatedly measuring the same target point,a nonlinear observation equation is established and then covered to linear equations using Taylor expansion.The weighted matrix is obtained according to the height of the measurement point and the camera optic axis pointing angle,and then the weighted least squares estimation is used to calculate the target position iteratively.Finally,the effectiveness and robustness of this method is verified by numerical simulation and flight test.The results show that this method can effectively improve the precision of target location. | Xu Cheng He Chenglong Huang Daqing | 2018 | Transactions of Nanjing University of Aeronautics and Astronautics2018，35，5： | 1 |
| 9 | User participation in an academic social networking service: A survey of open group users on mendeley显示文摘 | Jeng Wei He Daqing Jiang Jiepu | 2015 | Journal of the Association for Information Science and Technology2015，66，5： | 1 |
| 10 | Modeling and Parameter Estimation for Hydraulic System of Excavator's Arm显示文摘 | He Qinghua Hao Peng Zhang Daqing | 2008 | Journal of Central South University of Technology (English Edition)2008，15，3： | 1 |
| 11 | Modeling and pa- rameter estimation for hydraulic system of excavator' s arm 显示文摘 | He Qinghua Hao Peng Zhang Daqing | 2008 | Journal of Central South University of Technology (English Edition)2008，15，： | 1 |
| 12 | Multilingual needs and expectations in digital libraries显示文摘 | Dan Wu Daqing He Bo Luo | 2012 | The Electronic Library2012，，2： | 1 |
| 13 | Personalization via collaboration in Web retrieval systems:A context based approach 显示文摘 | Ayse Goker Daqing He | 2003 | Proceedings of the American Society ibr Information Science and Technology2003，40，1： | 1 |
| 14 | The state of iSchools: An analysis of academic research and graduate education 显示文摘 | Wu Dan He Daqing Jiang Jiepu | 2012 | Journal of Information Science2012，38，1： | 1 |
| 15 | User-assisted query translation for interactive cross -language informationretrieval 显示文摘 | Oard D W He Daqing Wang Jianqiang | 2008 | Information Processing & Management2008，44，1： | 1 |
| 16 | Series-connected DC based offshore wind farm and its control显示文摘 | He Daqing | 2013 | Shanghai:Shanghai Jiao Tong University2013，，： | 1 |
| 17 | Phase - shifting analysis on moire interferometry and its applications in electronic packaging显示文摘 | He Xiaoyuan Zou Daqing | 1998 | Optical Engineering1998，37，5： | 1 |
| 18 | Enhancing query translation with relevance feedback in translingual information retrieval 显示文摘 | He Daqing Wu Dan | 2011 | Information Proeessing & Management2011，47，1： | 1 |
| 19 | Multilingual needs and expectations in digital libraries: A survey of academic users with different languages显示文摘 | Dan Wu Daqing He Bo Luo | 2012 | The Electronic Library2012，，2： | 1 |
| 20 | Comparing soeial tags with subjecl headings on annotating books: A study comparing the information science domain in English and Chinese显示文摘 | Wu Dan He Daqing Qiu Jin | 2013 | Journal of Information Science2013，39，2： | 1 |
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