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| 1 | Regulatory effect and mechanism of gastrin and its antagonists on colorectal carcinoma显示文摘AIM To explore the effect and mechanism ofgastrin and its antagonists proglumide and somatostatin on coIorectal carcinoma and their clinical significance.METHODS A model of transplanted human colonic carcinoma was established from SW480cell line in gymnomouse body. The volume andweight of transplanted carcinoma was observedunder the effect of pentagatrin (PG), proglumide (PGL) and octapeptide somotostatin (SMS201-995, SMS). The cAMP content ot carcinoma cell was determined by redioimmunoassay and the DNA, protein content and cell cycle were determined by flow-cytometry. The amount of viabIe cells was determined by MTT colorimetric analysis, lP3 content was determined by radioimmunoassay, Ca2+ concentration in cell by fluorometry and PKC activity by isotopic enzymolysis. The expression of gastrin, c-myc,C-fos and rasP21 in 48 cases of colorectal carcinoma tissue was detected by the immunocytochemistry SP method. Argyrophilianucleolar organizer regions was determined withargyrophilia stain.RESULTS The volume, weight, cAMP, DNAand protein content in carcinoma cell, cellamount and proliferation index of S and G2Mphase in PG group were all significantly higher than those of control group. When PG was at theconcentration of 25 mg/ L, the amount of viablecells, lP3 content and Ca2+ concentration in celland membrane PKC activity in PG group weresigniticantly higher than those in control group;when PGL was at a concentration ot 32 mg/L,they dropped to the lowest level in PG (25 mg/L)+ PGL group, but without significant differencefrom the control group. The positive expression rate of gastrin, c-myc, c-fos and rasp21 in carcinoma tissue was 39 .6%, 54 .2%, 47. 9% and54 .2% resPectively and significantly higher than that in mucosa 3 cm and 6 cm adjacent tocarcinoma tissue and normal colorectal mucosa.The positive expression rate of gastrin of highlydifferentiated adenocarcinoma group was significantly higher than that of poorly differentiated and mucinous adenocarcinoma groups. The AgNORs count of carcinoma tissue was significantly higher than that in mucosa 3 cm and 6 cm adjacent to carcinoma tissue and normal colorectal mucosa; and the positive expression of c-myc and c-fos and the AgNORs count in gastrin-positive group was significantly higher than those in gastrin-negative group.CONCLUSION Pentagastrin has a promoting effect on the growth of transplanted human colonic carcinoma from SW480 cell line. PGL hasno obvious effect on the growth of human colonic carcinoma SW480 cell line, but couldinhibit the growth-promoting effect of PG on transplanted carcinoma. Somatostatin can not only inhibit the growth of transplanted human colonic carcinoma from SW480 cell line directlybut also depress the growth-promoting effect ofgastrin on the transplanted carcinoma. Somecolorectal carcinoma cells can produce and secrete gastrin through autocrine, highly-differentiated adenocarcinoma express the highest level gastrin. Endogenous gastrin can stimulate the cell division and proliferation of carcinoma cell and promote the growth of colorectal carcinoma regulating the expression of oncogene omyc, c-fos. Our study has provided experimental basis for the adjuvant treatment using gastrin antagonist such as PGL,somatostatin of patients with colorectalcarcinoma. | He SW Shen KQ He YJ Xie B Zhao YM | 1999 | World Journal of Gastroenterology1999，5，5： | 20 |
| 2 | Effects of radical cholecystectomy on nutritional and immune status in patients with gallbladder carcinoma显示文摘INTRODUCTION Carcinoma of the gallbladder is the most commonneoplasm in biliary tract,and its incidence has beenrising in recent years.The rate of correct diagnosisin early gallbladder carcinoma has been raised afterthe wide use of CT,ultrasound scans and frozensection examination.Now radical cholecystectomyis advocated as the best management for patientswith early gallbladder carcinoma.In the | Jiao XY Shi JS Wang JS Yang YJ He P | 2000 | World Journal of Gastroenterology2000，6，3： | 6 |
| 3 | Induction of mucosal and systemic immune response by single-dose oral immunization with biodegradable microparticles containing DNA encoding HBsAg显示文摘 | He XW Wang F Jiang L Li J Liu SK Xiao ZY Jin XQ Zhang YN He Y Li K Guo YJ Sun SH | 2005 | 第二军医大学学报2005，26，10： | 5 |
| 4 | Comparative proteomics analysis to an- nexin B1 DNA and protein vaccination in mice 显示文摘 | Li DA He Y Guo YJ | 2007 | Vaccine2007，25，5： | 1 |
| 5 | Cardioprotective effect of water and ethanol extract of Salvia miltiorrhiza in an ex- perimental model of myocardial infarction 显示文摘 | Zhou R He LF Li YJ | 2012 | Journal of ethnopharmacology2012，139，2： | 1 |
| 6 | The association between CYP2E1 polymorphisms and hepatotoxicity due to anti-tuberculosis drugs:a meta-analysis显示文摘 | Sheng YJ Wu G He HY | 2014 | Infect Genet Evol2014，24，： | 1 |
| 7 | Genetic ablation of apolipoprotein A-IV accelerates Alzheimer's disease pathogenesis in a mouse model显示文摘 | Cui YJ Huang MW He YB | 2011 | American Journal of Pathology2011，178，3： | 1 |
| 8 | Suppression of selenium-binding protein 1 in gastric cancer is associated with poor survival显示文摘 | Xia YJ Ma YY He XJ | | 0，，11： | 1 |
| 9 | DDB1 functions as a linker to recruit receptor WD40 proteins to CUL4-ROC1 ubiquitin ligases 显示文摘 | He YJ McCall CM Hu J | 2006 | Genes Dev2006，20，： | 1 |
| 10 | Effect of glycyrrhizin on theactivity of CYP3A enzyme in humans 显示文摘 | Tu JH He YJ Chen Y | 2010 | Eur J Clin Phar-waco/2010，66，8： | 1 |
| 11 | Cardioprotective effect of water and ethanolextract of Salvia miltiorrhiza in an experimental model of myocardial in-farction显示文摘 | Zhou R He LF Li YJ ef al | 2012 | J Ethnopharmacol2012，139，2： | 1 |
| 12 | Increased integrity of circulating cell-free DNA in plasma of patients with acute leukemia 显示文摘 | Gao YJ He YJ Yang ZL | 2010 | Clin Chem Lab Med2010，48，11： | 1 |
| 13 | Clinical utility of haptoglobin in combination with CEA, NSE and CYFRA21-1 for diagnosis of lung cancer显示文摘 | Wang B He YJ Tian YX | 2014 | Asian Pac J Cancer Prev2014，15，22： | 1 |
| 14 | Engineering of kex2 variants exhibiting altered substrate specificity显示文摘 | Rho SH Lee YJ | 2005 | Biochemical and Biophysical Research Communications2005，337，： | 1 |
| 15 | High intensity focused ultrasound ablation of goat liver in vivo:Pathologic changes of portal vein and the'heat-sink'effect显示文摘 | Jiang F HE M Liu YJ | 2013 | Ultrasonics2013，53，1： | 1 |
| 16 | The 3C protein of enterovirus 71 inhibits retinoid acid-inducible gene I-mediated interferon regulatory factor 3 activation and type I interferon responses 显示文摘 | Lei XB Liu XL Ma YJ Sun ZM Yang YW Jin Q He B Wang JW | 2010 | J Virol2010，84，16： | 1 |
| 17 | Inhibition of tumor growth and vasculogenic mimicry by curcumin through down-regula- tion of the EphA2/PI3K/MMP pathway in a murine choroidal melanoma model显示文摘 | Chen LX He YJ Zhao SZ | 2011 | Cancer Biol Ther2011，11，2： | 1 |
| 18 | Crystal structure of Bacillus cereusD-alanyl carrier protein Iigase ( DltA) in complex with ATP 显示文摘 | Osman KT Du LQ He YJ et a) | 2009 | J Mol Biol2009，388，2： | 1 |
| 19 | Effect of SLCOIB1 genetic polymorphism on the pharmacokinetics of nateglinide 显示文摘 | Zhang W He YJ Han CT | 2006 | Br J Clin Pharmacol2006，62，5： | 1 |
| 20 | Prognostic implications of type and density of tumour-infiltrating lymphocytes in gastric cancer显示文摘 | Lee HE Ghae SW Lee YJ | 2008 | Br J Gancer2008，99，10： | 1 |
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