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| 1 | Osteopontin increases hepatocellular carcinoma cell growth in a CD44 dependant manner显示文摘AIM:To investigate the role of osteopontin(OPN) and its splice variants in the proliferation of hepatocellular carcinoma(HCC).METHODS:The expression of OPN variants in HCC cell lines as well as HCC tissue samples and nontumour tissue was studied using polymerase chain reaction.OPN variant cDNAs were cloned into a mammalian expression vector allowing both transient expression and the production of stable OPN expressing cell lines.OPN expression was studied in these cells using Western blotting,immunofluoresnce and enzyme linked immunosorbent assay.A CD44 blocking antibody and siRNA targeting of CD44 were used to examine the role of this receptor in the OPN stimulated cell growth observed in culture.Huh-7 cells stably expressing either OPN-A,-B or-C were injected subcutaneously into the flanks of nude mice to observe in vivo tumour growth.Expression of OPN mRNA and protein in these tumours was examined using reverse transcriptionpolymerase chain reaction and immunohistochemistry.RESULTS:OPN is expressed in HCC in 3 forms,the full length OPN-A and 2 splice variants OPN-B and-C.OPN variant expression was noted in HCC tissue as well as cognate surrounding cirrhotic liver tissue.Expression of these OPN variants in the HCC derived cell line Huh-7 resulted in secretion of OPN into the culture medium.Transfer of OPN conditioned media to na ve Huh-7 and HepG2 cells resulted in significant cell growth suggesting that all OPN variants can modulate cell proliferation in a paracrine manner.Furthermore the OPN mediated increase in cellular proliferation was dependent on CD44 as only CD44 positive cell lines responded to OPN conditioned media while siRNA knockdown of CD44 blocked the proliferative effect.OPN expression also increased the proliferation of Huh-7 cells in a subcutaneous nude mouse tumour model,with Huh-7 cells expressing OPN-A showing the greatest proliferative effect.CONCLUSION:This study demonstrates that OPN plays a significant role in the proliferation of HCC through interaction with the cell surface receptor CD44.Modulation of this interaction could represent a novel strategy for the control of HCC. | Renee J Phillips Karla J Helbig Kylie H Van der Hoek Devanshi Seth Michael R Beard | 2012 | World Journal of Gastroenterology2012，18，26： | 12 |
| 2 | A comparative investigation of DNA adducts, DNA strand breaks and gene mutations induced by benzopyrene and (+/-)-anti-benzopyrene-7,8-diol 9,10-oxide in cultured human cells显示文摘 | Hanelt S Helbig R Hartmann A | 1997 | Mutat Res1997，390，12： | 2 |
| 3 | Video head im-pulse test or caloric irrigation- Contemporary diagnostic tests{or vestibular scKwannoma显示文摘 | Blodow A Helbig R Wichmann N | 2013 | HN〇2013，61，781： | 1 |
| 4 | Diagnosis of Echinococcus multilocularis infection by reverse-transcription polymerase chain reaction显示文摘 | Kern P Frosch P Helbig M Wechsler J G Usadel S Beckh K Kunz R Lucius R Frosch M 1995 | 1995 | Gas troenterology1995，109，2： | 1 |
| 5 | The proportion of CD3-CD4+T-cell population remained unaffected after corticosteroidstreatment for lymphocytic variant hypereosinophilic syndrome(L-HES)显示文摘 | Helbig G Wichary R Razny M | 2010 | Scand J Immunol2010，72，4： | 1 |
| 6 | Theoretical studies and spectroscopic investigations of ground and excited electronic states of thiophene oligomers显示文摘 | Colditz R Grebner D Helbig M | 1995 | Chem Phys1995，201，： | 1 |
| 7 | Effect of Oxygen Deprivation on Soil Hydrophobicity during Heating 显示文摘 | Bryant R Doerr S H Helbig M | 2005 | International Journal of Wildland Fire2005，14，： | 1 |
| 8 | The seasonal fertility of North Ameriman bison (Bison bison) bulls显示文摘 | HELBIG L WOODBURY M R HAIGH J C | 2007 | Anim Reprod Sci2007，97，34： | 1 |
| 9 | A comparative investigation of DNA adducts,DNA strand breaks and gene mutations induced by benzo(a)pyrene and anti-benzo(a)pyrene-7,8-diol9,10-oxide in cultured human cells显示文摘 | HANELT S HELBIG R HARTMANN A | 1997 | Mutat Res1997，390，12： | 1 |
| 10 | Isolated functional loss of the lateral semicircular canal in vestibular neuritis 显示文摘 | Blfidow A Helbig R Bloching M | 2013 | HNO2013，61，1： | 1 |
| 11 | SiC devices:physics and numerical simulation显示文摘 | Ruff M Mitlehner H Helbig R | 1994 | IEEE Trans Elec Dev1994，41，6： | 1 |
| 12 | A comparative investigation of DNA adducts, DNA strand breaks and gene mutations induced by benzo pyrene and ( + / - )-antl-benzo pyrene-7 , 8-diol 9 , 10-oxide in cultured human cells显示文摘 | HANELT S HELBIG R HARTMANN A | 1997 | Mnt Res1997，390，12： | 1 |
| 13 | Impurity Transitions in the Photoluminescence Spectra of SnO2 Solid State 显示文摘 | Blattner G Klingshrin C Helbig R | 1980 | Solid State Commun1980，，3： | 1 |
| 14 | A comparative investigation of DNA adducts,DNA strand breaks and gene mutations induced by benzo(a)pyrene and (±)-anti-benzopyrene -7,8 -diol 9,10-oxide in cultured human cells显示文摘 | HANELT S HELBIG R HARTMANN A | 1997 | Mutation Res1997，390，： | 1 |
| 15 | A comparative investigation of DNA adducts,DNA strand breaks and gene mutations induced by benzo(a)pyrene-7,8-9,10-oxide in cultured human cells显示文摘 | Hanelt S Helbig R Hartmann A | 1997 | Mutation Res1997，390，： | 1 |
| 16 | The role of viperin in the innate anti- viral response 显示文摘 | HELBIG K J BEARD M R | 2014 | J Mol Biol2014，426，6： | 1 |
| 17 | Sic device: Physics and numerical simulation 显示文摘 | Ruff M Milehner H Helbig R | 1994 | IEEE Transactions on Electron Devices1994，41，6： | 1 |
| 18 | Remote vital sign detection for rescue, security, and medical care by ultra-wideband pseudo-noise radar显示文摘 | Sachs J Helbig M Herrmann R | 2014 | Ad Hoc Networks2014，13，： | 1 |
| 19 | Scaffold attachment factor A (SAF-A) is concentrated in inactive X chromosome territories through its RGG domain显示文摘 | HELBIG R FACKELMAYER F O | 2003 | Chromosoma2003，112，4： | 1 |
| 20 | SiC devices:physics and numerical simulation 显示文摘 | MARTIN R MITLEHNER H HELBIG R | 1994 | IEEE Transactionon Electron Devices1994，41，6： | 1 |
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