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| 1 | Bcl-x_L and Myeloid cell leukaemia-1 contribute to apoptosis resistance of colorectal cancer cells显示文摘AIM: To explore the role of Bcl-xL and Myeloid cell leukaemia (Mcl)-1 for the apoptosis resistance of colorectal carcinoma (CRC) cells towards current treat-ment modalities. METHODS: Bcl-xL and Mcl-1 mRNA and protein ex-pression were analyzed in CRC cell lines as well as human CRC tissue by Western blot,quantitative PCRand immunohistochemistry. Bcl-xL and Mcl-1 protein expression was knocked down or increased in CRC cell lines by applying specific siRNAs or expression plas-mids,respectively. After modulation of protein expres-sion,CRC cells were treated with chemotherapeutic agents,an antagonistic epidermal growth factor recep-tor (EGFR1) antibody,an EGFR1 tyrosine kinase inhibi-tor,or with the death receptor ligand TRAIL. Apoptosis induction and cell viability were analyzed. RESULTS: Here we show that in human CRC tis-sue and various CRC cell lines both Bcl-xL and Mcl-1 are expressed. Bcl-xL expression was higher in CRC tissue than in surrounding non-malignant tissue,both on protein and mRNA level. Mcl-1 mRNA expression was significantly lower in ma-lignant tissues. However,protein expression was slightly higher. Viability rates of CRC cells were significantly decreased after knock down of Bcl-xL expression,and,to a lower extent,after knock down of Mcl-1 expression. Furthermore,cells with reduced Bcl-xL or Mcl-1 expression was more sensitive towards oxaliplatin-and irinotecan-induced apoptosis,and in the case of Bcl-xL also towards 5-FU-induced apoptosis. On the other hand,upregulation of Bcl-xL by transfec-tion of an expression plasmid decreased chemothera-peutic drug-induced apoptosis. EGF treatment clearly induced Bcl-xL and Mcl-1 expression in CRC cells. Apop-tosis induction upon EGFR1 blockage by cetuximab or PD168393 was increased by inhibiting Mcl-1 and Bcl-xL expression. More strikingly,CD95-and TRAIL-induced apoptosis was increased by Bcl-xL knock down. CONCLUSION: Our data suggest that Bcl-xL and,to a lower extent,Mcl-1,are important anti-apoptotic factors in CRC. Specific downregulation of Bcl-xL is a promising approach to sensitize CRC cells towards chemotherapy and targeted therapy. | Henning Schulze-Bergkamen Roland Ehrenberg Lothar Hickmann Binje Vick Toni Urbanik Christoph C Schimanski Martin R Berger Arno Schad Achim Weber Steffen Heeger Peter R Galle Markus Moehler | 2008 | World Journal of Gastroenterology2008，14，24： | 4 |
| 2 | Children's Expression of Voluntary Motion Events in English and Chinese显示文摘尽管人类对空间的认知可能具有一定的普遍性,但人类对同一空间事件的表达却因为语言的差异而千差万别。本研究旨在探讨英汉儿童如何表达基于动画的自主性运动事件。我们发现英语及汉语在空间表达上的类型学差异制约着儿童在小句层面上惯于表达的信息成分的类型及数量。汉语儿童在空间表达上的语句比同龄英语儿童的语句具有更高的信息密度,并且差异显著。这主要是因为汉语中的动词复合形式极大地促进了多重语义成分在同一小句中的同时体现。另外,英语儿童在3至5岁之间经历了空间表达方式上的重要发展,而相比之下汉语儿童的空间表达从3岁起就展现出了一些与成人的空间表达极为相似的特征。本研究揭示了语言独特性因素在语言习得中所起的重要作用。 | 纪瑛琳 Henri■tte Hendriks Maya Hickmann | 2011 | 外国语2011，34，4： | 3 |
| 3 | Fast preparation and gas chromatographic separation of lanthanide and actinide hexafluoroacetylacetonates 显示文摘 | Gruelich N Hickmann U Trautmann N Herrmann G | 1986 | Fresenius'' Zeitschrift fuer Analytische Chemie1986，323，3： | 1 |
| 4 | Application of air-mer- cury and oil-air capillary pressure data in the study of pore struc- ture and fluid distribution 显示文摘 | Picke11 J J Swanson B F Hickmann W B | 1996 | SPEJ1996，1，6： | 1 |
| 5 | Phytophthora plant destroyer 显示文摘 | Hickmann C J | 1958 | Transactions of the British Mycological Society1958，41，： | 1 |
| 6 | Uncoupling, metabolic inhibition and induction of mitochondrial permeability transition in rat liver mitochondria caused by the major long-chain hydroxyl monocarboxylic fatty acids accumulating in LCHAD deficiency显示文摘 | Hickmann FH Cecatto C Kleemann D | 2015 | Biochim Biophys Acta2015，1847，67： | 1 |
| 7 | Superluminal Effects and Negative Group Delays in Electrons,and Their Applications显示文摘 | SOLLI D CHIAO R Y HICKMANN A M | | 0，，： | 1 |
| 8 | Modulation instability in semiconductor-doped glass fibers with saturable-nonlinearity 显示文摘 | HICKMANN J M CAVALCANTI S B BORGES N M | 1993 | Opt Lett1993，18，3： | 1 |
| 9 | Interference of traveling nondiffracting beams显示文摘 | Chavez-Cerda S Meneses Nava M A Hickmann J M | 1998 | Opt Lett1998，23，24： | 1 |
| 10 | Conversion of residual glycerol from biodiesel synthesis into 1,3-propanediol by a new strain of Klebsiella pneumoniae显示文摘 | Daniele Misturini Rossi Elisangela Aquino de Souza Simone Hickmann Fl?res Marco Ant?nio Záchia Ayub | 2013 | Renewable Energy2013，，： | 1 |
| 11 | A Home-based Behavioral Intervention to Promote Walking in Sedentary Ethnic Minority Women: Project WALK显示文摘 | Chen A H Sallis J F Castro C M Lee R E Hickmann S A William C Martin J E | 1998 | Womens Health1998，4，1： | 1 |
| 12 | Optimization of cellulose-free xylanase activity produced by Bacillus coagulants BL69 in solid-cultivation显示文摘 | Júlio Xandro Heck Simone Hickmann Flores Plinho Francisco Hertz | 2005 | Process Biochemistry2005，40，1： | 1 |
| 13 | Statistical optimization of thermotolerant xylanase activity from Amazon isolated Bacillus circulars on solid-state cultivation显示文摘 | Júlio Xandro Heck Simone Hickmann Flores Plinho Francisco Hertz | 2006 | Bioresource Technology2006，97，15： | 1 |
| 14 | Decimal float-ing-point multiplication 显示文摘 | Erie MA Hickmann BJ Schulte MJ | 2009 | IEEE Transactions onComputers2009，58，7： | 1 |
| 15 | Light polarization control during free-space propagation using coherence显示文摘 | Vidal I Fonseca E J S Hickmann J M | 2011 | Phys Rev A2011，84，03： | 1 |
| 16 | A network-patch methodology for adapting agent-based models for directly transmitted disease to mosquito-borne disease 显示文摘 | Manore CA Hickmann KS Hyman JM | 2015 | J Biol Dyn2015，9，1： | 1 |
| 17 | Morphology and stability oF soil aggregates in a red yellow argisolunder different management long-term and secondary Atlantic forest显示文摘 | HICKMANN C DA COSTA L M SCHAEFER C E G R | 2011 | Revista Brasileira de Ci ^ ncia do Solo2011，35，6： | 1 |
| 18 | Highly active human pharmaceuticals in aquatic systems: A concept for their identification based on their mode of action 显示文摘 | CHRISTEN V HICKMANN S RECHENBERG B | 2010 | Aquatic Toxicology2010，96，3： | 1 |
| 19 | Effects of Leader Intelligence, Personality and Emotional Intelligence on Transformational Leadership and Managerial Performance 显示文摘 | Flavia Cavazotte Valter Moreno Mateus Hickmann | 2012 | The Leadership Quarterly2012，23，3： | 1 |
| 20 | Modulational instability in semiconductor-doped glass fibers with saturable nonlinearity 显示文摘 | Hickmann J M Cavalcanti S B Borges N M | 1993 | Opt Lett1993，18，3： | 1 |
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