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| 1 | 人卵透明带蛋白huZP3a^(22~176)和huZP3b^(177~348)肽段在大肠杆菌中的表达及其纯化显示文摘目的:探索将huZP3a22~348蛋白编码基因拆分成两段,分别表达的可行性,并研究表达产物的免疫原性。方法:用PCR技术, 将去N-端信号肽和C-端跨膜区的huZP322~348蛋白编码基因拆成大小相近的两段, 以完整阅读框的形式分别重组插入热诱导型pBV221的多克隆区。结果:大肠杆菌分别特异地表达了可单独或复合应用的huZP3a22~176和huZP3b177~348,在经过抗人ZP3不同抗原区合成肽抗体的蛋白印迹鉴定后, 用改良的制备性PAGE方法分离纯化这两种表达产物。同时用兔抗猪ZP IgGs蛋白印迹试验表明, huZP3a和pZP3b的几个共同线性抗原表位都存在于其肽链的前半区域。结论:通过基因重组技术可获得足够量的huZP3a和huZP3b,这为开展huZP3a和huZP3b的免疫原性以及huZP3诱发人精子顶体胞吐的功能域等研究奠定了基础。 | 徐万祥 何亚萍 洪爱真 季朝能 钱吉 王曙 谢毅 Elvira Hinsch Klaus-Dieter Hinsch | 2004 | 生殖与避孕2004，24，3： | 8 |
| 2 | 人卵透明带蛋白ZP3a和ZP3b肽段的免疫原性及其抗血清体外抑制人精子-半透明带结合显示文摘分析大肠杆菌表达的重组人卵透明带-3(r-huZP3)蛋白两个肽段(r-huZP3a22-176及r-huZP3b177-348)的免疫原性,比较两者抗血清体外抑制人精子-半透明带结合的能力。以制备性聚丙烯酰胺凝胶电泳(PAGE)纯化的大肠杆菌表达蛋白为抗原, 主动免疫新西兰兔产生抗huZP3a22-176及huZP3b177-348抗血清;通过ELISA测定比较两者的抗体应答水平:以蛋白印迹和免疫组化方法测定两者抗血清同重组表达蛋白、天然人卵ZP以及卵巢组织的反应性;通过竞争性半透明带结合试验 (hemizona assay,HZA)观察两者抗血清体外抑制人精子-ZP结合能力。结果显示:未与大分子蛋白载体耦联的r-huZP3a22-176 和r-huZP3b177-348抗原都在免疫兔中产生了较高的抗体滴度,而且它们的抗血清可识别大肠杆菌表达的各自重组ZP3肽以及人卵细胞表面天然ZP,两者抗血清也都能在体外抑制人精子-ZP结合。由此可见,r-huZP3a22-176及r-huZP3b177-348蛋白具有良好免疫原性,所产生抗血清也都显示出细胞和组织特异性。因此,单一或合并两个huZP3肽段均可作为抗原研制检测不明原因性不孕妇女中是否存在抗自身ZP抗体的诊断试剂盒,另外它们的抗血清也能用于鉴定已知huZP3表位肽段的最小B-细胞表位基序。 | 宋力雯 汪玉宝 倪崖 何亚萍 洪爱真 Elvira Hinsch Klaus-Dieter Hinsch 周思畅 袁玉英 石其贤 徐万祥 | 2005 | 生理学报2005，57，6： | 6 |
| 3 | The impact of early goal-directed fluid management on survival in an experimental model of severe acute pancreatitis显示文摘 | Constantin J. C. Trepte Kai A. Bachmann Jan H. Stork Till J. Friedheim Andrea Hinsch Matthias S. Goepfert Olliver Mann Jakob R. Izbicki Alwin E. Goetz Daniel A. Reuter | 2013 | Intensive Care Medicine2013，，4： | 2 |
| 4 | 22-gauge core vs 22-gauge aspiration needle for endoscopic ultrasound-guided sampling of abdominal masses显示文摘AIM To compare the aspiration needle(AN) and core biopsy needle(PC) in endoscopic ultrasound-guided fine needle aspiration(EUS-FNA) of abdominal masses.METHODS Consecutive patients referred for EUS-FNA were included in this prospective single-center trial. Each patient underwent a puncture of the lesion with both standard 22-gauge(G) AN(Echo Tip Ultra; Cook Medical, Bloomington, Indiana, United States) and the novel 22 G PC(Echo Tip Pro Core; Cook Medical, Bloomington, Indiana, United States) in a randomized fashion; histology was attempted in the PC group only. The main study endpoint was the overall diagnostic accuracy, including the contribution of histology to the final diagnosis. Secondary outcome measures included material adequacy, number of needle passes, and complications.RESULTS Fifty six consecutive patients(29 men; mean age 68 years) with pancreatic lesions(n = 38), lymphadenopathy(n = 13), submucosal tumors(n = 4), or others lesions(n = 1) underwent EUS-FNA using both of the needles in a randomized order. AN and PC reached similar overall results for diagnostic accuracy(AN: 88.9 vs PC: 96.1, P = 0.25), specimen adequacy(AN: 96.4% vs PC: 91.1%, P = 0.38), mean number of passes(AN: 1.5 vs PC: 1.7, P = 0.14), mean cellularity score(AN: 1.7 vs PC: 1.1, P = 0.058), and complications(none). A diagnosis on the basis of histology was achieved in the PC group in 36(64.3%) patients, and in 2 of those as the sole modality. In patients with available histology the mean cellularity score was higher for AN(AN: 1.7 vs PC: 1.0, P = 0.034); no other differences were of statistical significance.CONCLUSION Both needles achieved high overall diagnostic yields and similar performance characteristics for cytological diagnosis; histological analysis was only possible in 2/3 of cases with the new needle. | William Sterlacci Athanasios D Sioulas Lothar Veits Pervin G?nüllü Guido Schachschal Stefan Groth Mario Anders Christos K Kontos Theodoros Topalidis Andrea Hinsch Michael Vieth Thomas R?sch Ulrike W Denzer | 2016 | World Journal of Gastroenterology2016，22，39： | 2 |
| 5 | Fluoroscopy-based mutipanar image guidance for insertion of sacroiliac screws显示文摘 | Hinsche AF Giannoudis PV Smith RM | 2002 | J Clin Orthop Relate Res2002，，395： | 1 |
| 6 | Efficiency limiting factors of organic bulk heterojunction solar cells identified by electrical impedance spectroscopy显示文摘 | M. Glatthaar M. Riede N. Keegan K. Sylvester-Hvid B. Zimmermann M. Niggemann A. Hinsch A. Gombert | 2006 | Solar Energy Materials and Solar Cells2006，，5： | 1 |
| 7 | Fluoroscopy-based multiplanar image guidance for insertion of sacroiliacscrews显示文摘 | Hinsche AF Giannoudis PV Smith RM | 2002 | Clin Orthop Relat Res2002，395，： | 1 |
| 8 | Packagingbartlett pears in polyethylene film bags to reduce vibration injury in transit 显示文摘 | Slaughter D C Thompson J F Hinsch R T | 1998 | Transactions of the ASAE1998，41，10： | 1 |
| 9 | A Two-process View of Facebook Use and Relatedness Need-satisfaction: Disconnection Drives Use, and Connection Rewards It显示文摘 | Sheldon K M Abad N Hinsch C | 2011 | Journal of Personality and Social Psychology2011，100，4： | 1 |
| 10 | VDAC2 ( porin - 2) expression pattern and localization in the bovine testis 显示文摘 | Hinsch KD Asmarinah Hinsch E | 2001 | Biochim Biophys Acta2001，1518，3： | 1 |
| 11 | The Arabidopsis RPS4 bacterial-resistance gene is a member of the TIR-NBS-LRR family of disease-resistance genes显示文摘 | Gassmann W Hinsch M E Staskawicz B J | 1999 | Plant J1999，20，3： | 1 |
| 12 | Simultaneous bilateralfemoral fractures : systemic complications in 14 cases显示文摘 | Giannoudis PV Cohen A Hinsche A | 2000 | Int Orthop2000，24，5： | 1 |
| 13 | Morphology of the reproductive tract and seasonality of reproduction in the golden crab Geryon fenneri from the eastern Gulf of Mexico显示文摘 | Hinsch G W | 1988 | J Crustacean Biol1988，8，2： | 1 |
| 14 | Assessment of vibration injury to Bartlett pears显示文摘 | Slaughter D C Hinsch R T Thompson J F | 1993 | Transactions of the ASAE1993，36，4： | 1 |
| 15 | Routine glucose determination in serum by use of an immobilized glucose dehydrogenase nylon-tube reactor显示文摘 | Sundaram PV Blumenberg B Hinsch W | 1979 | Clin chem1979，25，8： | 1 |
| 16 | Vibration of fresh fruits and vegetables during refrigerated truck transport显示文摘 | Hinsch R T Slaughter D C Craig W L | 1993 | Transactions of the ASAE1993，36，4： | 1 |
| 17 | Sol-gel glasses: a new material for solar fluorescent planar concentrators? 显示文摘 | Hinsch A Zastrow A Wittwer V | 1990 | Solar Energy Materials1990，21，2： | 1 |
| 18 | Ultrastructure of the Y - organ of Cancer antennari us in normal and de - eyestalked crabs 显示文摘 | Hinsch G W | 1980 | Morphol1980，163，： | 1 |
| 19 | 显示文摘 | Hinsch A Kroon J M Kern R | 2001 | J Prog Photovolt: Res Appl2001，9，10： | 1 |
| 20 | Voltage-dependent anion-selective channels VDAC2 and VDAC3 are abundant proteins in bovine outer dense fibers, a cytoskeletal component of the sperm flagellum 显示文摘 | HINSCH K D De-PINTO V AIRES V A | 2004 | J Biol Chem2004，279，15： | 1 |
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