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您的检索式：作者名="Hisamits Tadashi"
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| 1 | Celastrus Orbiculatus Extract Inhibits Tumor Angiogenesis by Targeting Vascular Endothelial Growth Factor Signaling Pathway and Shows Potent Antitumor Activity in Hepatocarcinomas in Vitro and in Vivo显示文摘客观 Celastrus orbiculatus Thunb。对癌症和煽动性的疾病作为疗法在中国被用于几千年。这研究试图调查是否 C。orbiculatus 摘录(COE ) 能禁止 angiogenesis,它是在肿瘤生长,侵略,和转移的枢轴的步。在这的方法学习,从 C 的茎的摘录。orbiculatus 被使用。老鼠肝的癌房间(Hepa1-6 ) 在不同无毒的集中(10, 20, 40, 80,和 160 g/mL ) 与 COE 被对待。脉管的 endothelial 生长因素(VEGF ) 的 mRNA 和蛋白质表示层次被反向的抄写聚合酶链反应(RT-PCR ) 和西方的污点分别地检测;活跃部分进一步为任何 antiangiogenic 效果在 C57BL/6 老鼠和人的脐的静脉 endothelial 房间(HUVEC ) 上被测试。结果 COE 显著地禁止了增长并且在 Hepa1-6 房间导致了 apoptosis 并且在 mRNA 和蛋白质层次禁止了 VEGF 表示。而且,这个代理人以一种剂量依赖者方式在主要有教养的 HUVEC 禁止了象毛状一样结构的形成。在 vivo, COE 显著地与低不利效果减少了稳固的肿瘤的体积和重量并且减少肿瘤 angiogenesis。在摘要的结论, COE 能习惯于对待肝的癌。COE 的 antitumor 活动的机制可能由于它对由指向 VEGF 蛋白质的肿瘤 angiogenesis 的效果。 | 钱亚云 张华 侯莹 员林 李国青 郭试瑜 Hisamits Tadashi 刘延庆 | 2012 | Chinese Journal of Integrative Medicine2012，18，10： | 27 |
| 2 | Celastrus orbiculatus extract induces mitochondrial-mediated apoptosis in human hepatocellular carcinoma cells显示文摘OBJECTIVE:To investigate the apoptotic effects and underlying molecular mechanisms of Celastrus orbiculatus(C.orbiculatus) extract in human hepatocellular carcinoma cells.METHODS:Human hepatocellular carcinoma cells(HCCLM6) were treated with C.orbiculatus extract(COE) at different nontoxic concentrations(10,20,40,80,and 160 μg/mL).The effect of COE on HC-CLM6 viability was examined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) assays.Cellular apoptosis following COE treatment was assessed by flow cytometry and western blot analysis.RESULTS:COE significantly inhibited cell viability and induced apoptosis of HCCLM6 cells in a dose-dependent manner.Apoptosis was accompanied by increased Bax expression and decreased Bcl-2 expression.In addition,COE treatment led to the release of cytochrome c,activation of caspase-3,and cleavage of poly(ADP-ribose) polymerase(PARP).Furthermore,activation of extracellular signal-regulated kinase(ERK),p38 kinase,and c-Jun N-terminal kinase(JNK) phosphorylation,and down-regulation of Akt phosphorylation was observed.CONCLUSION:COE induces mitochondrial-mediated,caspase-dependent apoptosis in HCCLM6 cells,which might be attributed to the activation of mitogen-activated protein kinase(MAPK) and inhibition of Akt signaling pathways.These data suggest that COE may be a potential treatment for human hepatocellular carcinoma. | Hua Zhang Yayun Qian Yanqing Liu Guoqing Li Pingfang Cui Yaodong Zhu Hui Ma Xue Ji Shiyu Guo Hisamits Tadashi | 2012 | Journal of Traditional Chinese Medicine2012，32，4： | 18 |
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