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| 1 | 国外环境生物技术的发展和展望显示文摘环境生物技术在社会的发展中起着越来越重要的积极作用,相关产业也随之迅速发展起来。环境治理要依赖对生物,尤其是微生物及其生理、生化特性的了解和认识,从而可以对其生理、生化和遗传方面的性能加以利用。在这方面,分离解毒微生物及阐明毒物降解过程和机理仍是现在研究的焦点。与此同时,对遗传基因方面的研究和利用还有许多研究工作待进行。监测和跟踪微生物已进入到了分子水平,而传感监测方面已有一些可喜的成果正在向生产转化。生物修复作为消除污染的手段正在治理中发挥巨大的作用,但环境保护的根本应是在于进行无污染产生的生产,也称之为绿色生产。未来的社会中,环境生物技术仍将对社会产生巨大的影响,对环境保护起到重要的作用,特别是在新型生物能源的开发和探索方面。本文同时也对世界不同地区在环境生物技术的发展及其特点进行了综述。 | 顾继东 hkucc.hku hk | 1999 | 生物技术通报1999，15，6： | 35 |
| 2 | Voxel-based detection of white matter abnormalities in mild Alzheimer disease显示文摘 | Xie S Xiao JX Gong GL Zang YF Wang YH Wu HK Jiang XX | 2006 | 中国生物学文摘2006，20，10： | 20 |
| 3 | Calcium phosphate cements for bone engineering and their biological properties显示文摘Calcium phosphate cements(CPCs) are frequently used to repair bone defects. Since their discovery in the 1980 s, extensive research has been conducted to improve their properties, and emerging evidence supports their increased application in bone tissue engineering. Much effort has been made to enhance the biological performance of CPCs, including their biocompatibility, osteoconductivity, osteoinductivity, biodegradability,bioactivity, and interactions with cells. This review article focuses on the major recent developments in CPCs,including 3 D printing, injectability, stem cell delivery, growth factor and drug delivery, and prevascularization of CPC scaffolds via co-culture and tri-culture techniques to enhance angiogenesis and osteogenesis. | Hockin HK Xu Ping Wang Lin Wang Chongyun Bao Qianming Chen Michael D Weir Laurence C Chow Liang Zhao Xuedong Zhou Mark A Reynolds | 2017 | Bone Research2017，5，4： | 14 |
| 4 | Effects of quaternary ammonium chain length on the antibacterial and remineralizing effects of a calcium phosphate nanocomposite显示文摘Composites containing nanoparticles of amorphous calcium phosphate(NACP)remineralize tooth lesions and inhibit caries.A recent study synthesized quaternary ammonium methacrylates(QAMs)with chain lengths(CLs)of 3–18 and determined their effects on a bonding agent.This study aimed to incorporate these QAMs into NACP nanocomposites for the first time to simultaneously endow the material with antibacterial and remineralizing capabilities and to investigate the effects of the CL on the mechanical and biofilm properties.Five QAMs were synthesized:DMAPM(CL3),DMAHM(CL6),DMADDM(CL12),DMAHDM(CL16),and DMAODM(CL18).Each QAM was incorporated into a composite containing 20%NACP and 50%glass fillers.A dental plaque microcosm biofilm model was used to evaluate the antibacterial activity.The flexural strength and elastic modulus of nanocomposites with QAMs matched those of a commercial control composite(n 5 6;P.0.1).Increasing the CL from 3 to 16 greatly enhanced the antibacterial activity of the NACP nanocomposite(P,0.05);further increasing the CL to 18 decreased the antibacterial potency.The NACP nanocomposite with a CL of 16 exhibited biofilm metabolic activity and acid production that were 10-fold lesser than those of the control composite.The NACP nanocomposite with a CL of 16 produced 2-log decreases in the colony-forming units(CFU)of total microorganisms,total streptococci,and mutans streptococci.In conclusion,QAMs with CLs of 3–18 were synthesized and incorporated into an NACP nanocomposite for the first time to simultaneously endow the material with antibacterial and remineralization capabilities.Increasing the CL reduced the metabolic activity and acid production of biofilms and caused a 2-log decrease in CFU without compromising the mechanical properties.Nanocomposites exhibiting strong anti-biofilm activity,remineralization effects,and mechanical properties are promising materials for tooth restorations that inhibit caries. | Ke Zhang Lei Cheng Michael D Weir Yu-Xing Bai Hockin HK Xu | 2016 | International Journal of Oral Science2016，8，1： | 13 |
| 5 | 苦瓜果汁对链脲霉素诱导的大鼠糖尿病的影响显示文摘 | Eric.,HK 李宗友 | 1992 | 国外医学(中医中药分册)1992，14，1： | 10 |
| 6 | A novel protein-repellent dental composite containing 2-methacryloyloxyethyl phosphorylcholine显示文摘Secondary caries due to biofilm acids is a primary cause of dental composite restoration failure.To date,there have been no reports of dental composites that can repel protein adsorption and inhibit bacteria attachment.The objectives of this study were to develop a protein-repellent dental composite by incorporating 2-methacryloyloxyethyl phosphorylcholine(MPC) and to investigate for the first time the effects of MPC mass fraction on protein adsorption,bacteria attachment,biofilm growth,and mechanical properties.Composites were synthesized with 0(control),0.75%,1.5%,2.25%,3%,4.5%and 6%of MPC by mass.A commercial composite was also tested as a control.Mechanical properties were measured in three-point flexure.Protein adsorption onto the composite was determined by the microbicinchoninic acid method.A human saliva microcosm biofilm model was used.Early attachment at 4 h,biofilm at 2 days,live/dead staining and colony-forming units(CFUs) of biofilms grown on the composites were investigated.Composites with MPC of up to 3%had mechanical properties similar to those without MPC and those of the commercial control,whereas 4.5%and 6%MPC decreased the mechanical properties(P<0.05).Increasing MPC from 0 to 3%reduced the protein adsorption on composites(P<0.05).The composite with 3%MPC had protein adsorption that was 1/12 that of the control(P<0.05).Oral bacteria early attachment and biofilm growth were also greatly reduced on the composite with 3%MPC,compared to the control(P<0.05).In conclusion,incorporation of MPC into composites at 3%greatly reduced protein adsorption,bacteria attachment and biofilm CFUs,without compromising mechanical properties.Protein-repellent composites could help to repel bacteria attachment and plaque build-up to reduce secondary caries.The protein-repellent method might be applicable to other dental materials. | Ning Zhang Chen Chen Mary AS Melo Yu-Xing Bai Lei Cheng Hockin HK Xu | 2015 | International Journal of Oral Science2015，7，2： | 8 |
| 7 | One-year water-ageing of calcium phosphate composite containing nano-silver and quaternary ammonium to inhibit biofilms显示文摘Dental composites are commonly used restorative materials; however, secondary caries due to bio?lm acids remains a major problem. The objectives of this study were(1) to develop a composite containing quaternary ammonium dimethacrylate(QADM),nanoparticles of silver(NAg), and nanoparticles of amorphous calcium phosphate(NACP), and(2) to conduct the ?rst investigation of the mechanical properties, bio?lm response and acid production vs water-ageing time from 1 day to 12 months.A 4 × 5 design was utilized, with four composites(NACP-QADM composite, NACP-NAg composite, NACP-QADM-NAg composite,and a commercial control composite), and ?ve water-ageing time periods(1 day, and 3, 6, 9, and 12 months). After each waterageing period, the mechanical properties of the resins were measured in a three-point ?exure, and antibacterial properties were tested via a dental plaque bio?lm model using human saliva as an inoculum. After 12 months of water-ageing, NACP-QADMNAg had a ?exural strength and elastic modulus matching those of the commercial control(P40.1). Incorporation of QADM or NAg into the NACP composite greatly reduced bio?lm viability, metabolic activity and acid production. A composite containing both QADM and NAg possessed a stronger antibacterial capability than one with QADM or NAg alone(Po0.05). The anti-bio?lm activity was maintained after 12 months of water-ageing and showed no signi?cant decrease with increasing time(P40.1). In conclusion, the NACP-QADM-NAg composite decreased bio?lm viability and lactic acid production, while matching the loadbearing capability of a commercial composite. There was no decrease in its antibacterial properties after 1 year of water-ageing.The durable antibacterial and mechanical properties indicate that NACP-QADM-NAg composites may be useful in dental restorations to combat caries. | Lei Cheng Ke Zhang Chen-Chen Zhou Michael D Weir Xue-Dong Zhou Hockin HK Xu | 2016 | International Journal of Oral Science2016，8，3： | 8 |
| 8 | Voxel-based detection of white matter abnormalities in mild Alzheimer disease显示文摘 | Xie S Xiao JX Gong GL Zang YF Wang YH Wu HK Jiang XX | 2006 | 中国生物学文摘2006，20，8： | 8 |
| 9 | Apolipoprotein E, angiotensin-converting enzyme and kallikrein gene polymorphisms and the risk of Alzheimer's disease and vascular dementia显示文摘 | Wang HK Fung HC Hsu WC Wu YR Lin JC Ro LS Chang KH Hwu FJ Hsu Y Huang SY Lee-Chen GJ Chen CM | 2006 | 中国生物学文摘2006，20，8： | 8 |
| 10 | 维生素D对心力衰竭患者全因死亡率的影响:4000U/d维生素D口服3年随机临床试验结果显示文摘循环25-羟基维生素D[25-hydroxyvitamin D,25(OH)D]〈75nmol/L与死亡率风险的非线性增加相关。这种低25(OH)D水平在心力衰竭患者中很常见。因此,Zittermann等进行研究检查口服维生素D补充剂能否降低晚期心力衰竭患者的死亡率。 | 刘莉 叶鹏 Zittermann A Ernst JB Prokop S Fuchs U Dreier J Kuhn J Knabbe C Birschmann I Schulz U Berthold HK Pilz S Gouni-Berthold I Gummert JF Dittrich M Brgermann J | 2017 | 中华高血压杂志2017，25，10： | 7 |
| 11 | 雌激素通过调节骨髓来源的成骨细胞所产生的细胞因子抑制破骨细胞功能显示文摘目的 通过观察雌激素对骨髓来源的成骨细胞所产生的细胞因子的调节作用 ,探讨雌激素抑制破骨细胞功能的机制。方法 在诱导小鼠骨髓细胞分化为成骨细胞后 ,于成骨细胞培养中加入雌激素 ,应用抗体荧光免疫标记法检测成骨细胞培养液中IL 1,IL 6和TNF α水平。结果与对照组比较 ,经 0 0 1~ 1nM雌激素处理后 ,成骨细胞所产生的IL 1和IL 6水平呈浓度依赖性下降 ,TNF α水平则无明显变化。结论 雌激素具有调节成骨细胞分泌细胞因子功能 。 | 曲强 万新海 陈翠珠 Vnnen HK | 2002 | 中国骨质疏松杂志2002，8，1： | 6 |
| 12 | 腰骶神经根减压手术前、后及术中躯体感觉诱发电位与临床预后的相关性显示文摘观察腰骶神经根病变患者减压手术中 DSEP改善程度与术后临床症状改善之间的相关性。对 2 2例经 CT或 MRI检查证实的腰骶神经根压迫症患者 ,在术前及术后 9周 (8~ 10周 )进行临床随访检查。结果减压手术中 DSEP的波幅增高 ,术后临床症状明显改善 (P<0 .0 1) ;术后 DSEP波幅较术前增高 (P<0 .0 5 ,P<0 .0 1) ;潜伏期缩短 (P<0 .0 5 ,P<0 .0 1)。分析认为 ,脊神经根减压手术前 DSEP变化与术后临床症状改善呈正相关 ;术中 | 周晖 贺斌 赵瑛 HK WONG | 2003 | 第二军医大学学报2003，24，2： | 6 |
| 13 | Screening and identification of mimotopes of the major shrimp allergen tropomyosin using one-bead-one- compound peptide libraries显示文摘one-bead-one-compound (OBOC ) 组合的肽图书馆是一个强大的工具识别 ligand 和受体相互作用。这里,我们使用了 OBOC 图书馆技术识别对主要贝壳变应原 tropomyosin 的免疫球蛋白 E (IgE ) epitopes 特定的 mimotopes。有 8-12 氨基酸残余的 OBOC 肽图书馆为 tropomyosin 的 IgE mimotopes 与贝壳过敏症从病人与浆液样品被屏蔽。25 mimotopes 从屏蔽被识别,他们到 tropomyosin 特定的 IgE 的有约束力的反应被肽 ELISA 证实。这些 mimotopes 能基于顺序相同被划分成七簇,并且由聚类的 mimotopes 的 EpiSearch 印射的 epitope 被执行描绘并且证实 mimotopes 的有效性。五从六预言的 epitopes 被发现与 tropomyosin 的以前识别的 epitopes 重叠。为了推进,证实 mimotopes 的模仿潜力, BALB/c 老鼠被使免疫, mimotopes 结合了到锁眼恋栈者 hemocyanin 和 assayed 让他们的能力导致 tropomyosin 特定的抗体。那收到的 mimotope 免疫被发现有 tropomyosin 特定的免疫球蛋白 G 的提高的水平的 BALB/c 老鼠,然而并非收到了无关的 mimotope 的老鼠。这研究用整个 sera 开创 OBOC 图书馆的成功的申请屏蔽并且识别多重虾变应原 mimotopes 并且验证他们在 vitro 使用的模仿潜力,在 vivo,并且在 silico 方法。 | Nicki YH Leung Christine YY Wai Marco HK Ho Ruiwu Liu Kit S Lam Jin Jun Wang Shang An Shu Ka Hou Chu Patrick SC Leung | 2017 | Cellular & Molecular Immunology2017，14，3： | 6 |
| 14 | Colorectal liver metastases: Current management and future perspectives显示文摘The liver is the commonest site of metastatic disease for patients with colorectal cancer,with at least 25%developing colorectal liver metastases(CRLM)during the course of their illness.The management of CRLM has evolved into a complex field requiring input from experienced members of a multi-disciplinary team involving radiology(cross sectional,nuclear medicine and interventional),Oncology,Liver surgery,Colorectal surgery,and Histopathology.Patient management is based on assessment of sophisticated clinical,radiological and biomarker information.Despite incomplete evidence in this very heterogeneous patient group,maximising resection of CRLM using all available techniques remains a key objective and provides the best chance of long-term survival and cure.To this end,liver resection is maximised by the use of downsizing chemotherapy,optimisation of liver remnant by portal vein embolization,associating liver partition and portal vein ligation for staged hepatectomy,and combining resection with ablation,in the context of improvements in the functional assessment of the future remnant liver.Liver resection may safely be carried out laparoscopically or open,and synchronously with,or before,colorectal surgery in selected patients.For unresectable patients,treatment options including systemic chemotherapy,targeted biological agents,intraarterial infusion or bead delivered chemotherapy,tumour ablation,stereotactic radiotherapy,and selective internal radiotherapy contribute to improve survival and may convert initially unresectable patients to operability.Currently evolving areas include biomarker characterisation of tumours,the development of novel systemic agents targeting specific oncogenic pathways,and the potential reemergence of radical surgical options such as liver transplantation. | Jack Martin Angelica Petrillo Elizabeth C Smyth Nadeem Shaida Samir Khwaja HK Cheow Adam Duckworth Paula Heister Raaj Praseedom Asif Jah Anita Balakrishnan Simon Harper Siong Liau Vasilis Kosmoliaptsis Emmanuel Huguet | 2020 | World Journal of Clinical Oncology2020，11，10： | 5 |
| 15 | 补充甲状腺激素对亚临床甲状腺功能减退患者死亡率的影响显示文摘最新发表的一项荟萃分析入选了5项观察性研究和两项随机对照试验,共21055例成人患者。结果发现,在总人群中,甲状腺激素治疗与全因死亡或心血管死亡无关。但亚组分析显示,在年龄小于65岁的人群中,甲状腺激素治疗明显降低患者的全因死亡率(相对风险=0.50,95%可信区间:0.29~0.85,P=0.011)和心血管死亡率(相对风险=0.54,95%可信区间:0.37~0.80,P=0.002)。 | 杨菊红(摘译) Peng CC Huang HK Wu BB | 2021 | 国际内分泌代谢杂志2021，41，1： | 5 |
| 16 | Effect of anti-biofilm glass–ionomer cement on Streptococcus mutans biofilms显示文摘Dental restorative materials with antimicrobial properties can inhibit bacterial colonization, which may result in a reduction of caries at tooth-filling interaction zones. This study aimed to develop antibacterial glass–ionomer cements(GIC) containing a quaternary ammonium monomer(dimethylaminododecyl methacrylate, DMADDM), and to investigate their effect on material performance and antibacterial properties. Different mass fractions(0, 1.1% and 2.2%) of DMADDM were incorporated into the GIC. The flexure strength, surface charge density, surface roughness and fluoride release were tested. A Streptococcus mutans biofilm model was used. Exopolysaccharides(EPS) staining was used to analyze the inhibitory effect of DMADDM on the biofilm matrix. In addition, biofilm metabolic activity, lactic acid metabolism and the expression of glucosyltransferase genes gtf B, gtf C and gtf D were measured. GIC containing 1.1% and 2.2% DMADDM had flexural strengths matching those of the commercial control(P40.1). DMADDM was able to increase the surface charge density but reduced surface roughness(Po0.05). The incorporation of 1.1% and 2.2% DMADDM elevated the release of fluoride by the GIC in the first 2 days(Po0.05). The novel DMADDM-modified GIC significantly reduced biofilm metabolic activity(Po0.05) and decreased lactic acid production(Po0.05). The quantitative polymerase chain reaction(q PCR) results showed that the expression of gtf B, gtf C and gtf D decreased when mass fractions of DMADDM increased(Po0.05). EPS staining showed that both the bacteria and EPS in biofilm decreased in the DMADDM groups. The incorporation of DMADDM could modify the properties of GIC to influence the development of S. mutans biofilms. In this study, we investigated the interface properties of antibacterial materials for the first time. GIC containing DMADDM can improve material performance and antibacterial properties and may contribute to the better management of secondary caries. | Su-Ping Wang Yang Ge Xue-Dong Zhou Hockin HK Xu Michael D Weir Ke-Ke Zhang Hao-Hao Wang Matthias Hannig Stefan Rupf Qian Li Lei Cheng | 2016 | International Journal of Oral Science2016，8，2： | 5 |
| 17 | 空气喷磨和酸蚀与树脂突超微结构的关系显示文摘目的 体外评价 5种处理方法对树脂突超微结构的影响。方法 对照组 :对 1颗离体磨牙常规预防性树脂充填。其余 4颗磨牙用空气喷磨处理 ,分别用 5 0 μ和 2 7μ氧化铝微粒 ,酸处理其中 2颗磨牙。窝洞用复合树脂充填后 ,用 10 %盐酸溶解牙釉质 ,扫描电镜观测树脂突超微结构。结果 对照组呈典型蜂窝状结构。空气喷磨的树脂突呈不规则结构 ,结合酸蚀的树脂突表面更加粗糙。 | 傅柏平 Hannig M 吴求亮 Albers HK | 2000 | 现代口腔医学杂志2000，14，1： | 4 |
| 18 | 短纤维在橡胶中的补强作用及其应用显示文摘精制纤维素纤维具有独特的尺寸和物理特性,可为橡胶提供良好的加工性能及补强性能。通过对纤维取向的控制,制品可制成在某个方向刚性较大,而在另一方面向则是柔性的。它可作为其它短纤维,如棉和聚酯纤维的替代品使用。TCF亦是机织织物良好的替代品。 | Huan,HK 姚琳 | 1999 | 轮胎研究与开发1999，，2： | 4 |
| 19 | Risk factors and natural history of breast cancer in younger Chinese women显示文摘AIM: To investigate the age differences in the risk factors, clinicopathological characteristics and patterns of treatment of female breast cancer patients. METHODS: Seven thousand one hundred and fiftytwo women with primary breast cancer from the Hong Kong Breast Cancer Registry were recruited after receiving patients' consent, they were asked to complete standardized questionnaires which captured their sociodemographic characteristics and risk factors associated with breast cancer development. Among them, clinicopathological data and patterns of treatment were further collected from medical records of 5523 patients with invasive breast cancers. Patients were divided into two groups according to the age at diagnosis: younger(< 40 years old) vs older patients(≥ 40 years old) for subsequent analyses.RESULTS: Analysis on the sociodemographic characteristics and exposure to risk factors were performed on 7152 women with primary breast cancer and the results revealed that younger patients were more likely to have unhealthy lifestyles; these include a lack of exercise(85.4% vs 73.2%, P < 0.001), having high stress in life(46.1% vs 35.5%, P < 0.001), having dairy/meat-rich diets(20.2% vs 12.9%, P < 0.001),having alcohol drinking habit(7.7% vs 5.2%, P = 0.002). Younger patients were also more likely to have hormone-related risk factors including nulliparity(43.3% vs 17.8%, P < 0.001) and an early age at menarche(20.7% vs 13.2%, P < 0.001). Analyses on clinicopathological characteristics and patterns of treatment were performed on 5523 women diagnosed with invasive breast cancer. The invasive tumours in younger patients showed more aggressive pathological features such as having a higher percentage of grade 3 histology(45.7% vs 36.5%, P < 0.001), having a higher proportion of tumours with lymphovascular invasion(39.6% vs 33.2%, P = 0.003), and having multifocal disease(15.7% vs 10.3%, P < 0.001); they received different patterns of treatment than their older counterparts.CONCLUSION: Younger patients in Hong Kong are more likely to encounter risk factors associated with breast cancer development and have more aggressive tumours than their older counterparts. | Winnie Yeo Hang-Mei Lee Amy Chan Emily YY Chan Miranda CM Chan Keeng-Wai Chan Sharon WW Chan Foon-Yiu Cheung Polly SY Cheung Peter HK Choi Josette SY Chor William WL Foo Wing-Hong Kwan Stephen CK Law Lawrence PK Li Janice WH Tsang Yuk Tung Lorna LS Wong Ting-Ting Wong Chun-Chung Yau Tsz-Kok Yau Benny CY Zee | 2014 | World Journal of Clinical Oncology2014，5，5： | 4 |
| 20 | 冠状动脉狭窄的16层螺旋CT造影检查显示文摘目的以常规X线冠状动脉造影为标准,评价16层螺旋CT(MSCT)无创性检查冠状动脉及诊断冠状动脉狭窄的价值。资料与方法65例临床初诊为冠心病,无冠状动脉成形术和搭桥术史的患者,在冠状动脉16层MSCT造影检查后,回顾性重建心电门控轴位图像,并采用容积成像(VR)、多平面重建(MPR)、曲面MPR、最大密度投影(MIP)等后处理方法,对所有冠状动脉及其分支进行重建,统计可供临床评价的、管径≥1.5mm的冠状动脉段,以选择性冠状动脉造影(SCA)为标准,对比分析MSCT诊断冠状动脉显著性狭窄(管腔平均直径缩小超过50%)的准确性。其中8例在检查前心率超过80次/min的患者使用了β受体阻滞剂。结果93%的冠状动脉节段和94%的冠状动脉主干可供评价,不能评价的主要原因分别为心脏运动伪影(58%),致密钙化(28%)和管腔显影不良(14%)。除外不能评价的冠状动脉,按节段和主干分类,MSCT诊断冠状动脉显著性狭窄的敏感性和特异性分别达到92%、98%和95%、97%。结论无需常规使用β受体阻滞剂,16层MSCT冠状动脉造影即可获得较好的图像质量用于诊断冠状动脉狭窄,是一种值得临床医师信赖的检查冠状动脉有无狭窄的非创伤性方法。 | 史河水 韩萍 孔祥泉 冯敢生 童兴 Martin HK Hoffmann | 2005 | 临床放射学杂志2005，24，9： | 4 |
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