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| 1 | Antihepatoma effect of alpha-fetoprotein antisense phosphorothioate oligodeoxyribonucleotides in vitro and in mice显示文摘AIM To evaluate antihepatoma effect ofantisense phosphorothioate oligodeo-xyribonucleotides (S-ODNs) targeted to alpha-fetoprotein (AFP) genes in vitro and in nudemice.METHODS AFP gene expression was examinedby immunocytochemical method or enzyme-linked immunosorbent assay. Effect of S-ODNson SMMC-7721 human hepatoma cell growth invitro was determined using microculturetetrazolium assay. In vivo antitumor activitiesof S-ODNs were monitored by measuring tumorweight differences in treated and control micebearing SMMC-7721 xenografts. Induction of cellapoptosis was evaluated by fluorescence-activated cell sorter (FACS) analysis.RESULTS Antisense S-ODN treatment led toreduced AFP gene expression. Specificantisense S-ODNs, but not control S-ODNs,inhibited the growth of heaptoma cells in vitro.In vivo. only antisense S-ODNs exhibitedobvious antitumor activities. FACS analysisrevealed that the growth inhibition by antisenseS. ODNs was associated with their cell apoptosisinduction.CONCLUSION Antisense S-ODNs targeted toAFP genes inhibit the growth of human hepatomacells and solid hepatoma, which is related totheir cell apoptosis induction. | Xing Wang Wang~1 Jin Hui Yuan~1 Ru Gang Zhang~1 Li Xia Guo~1 Yong Xie~2 Hong Xie~1 ~1Department of Biotherapy,Shanghai Institute of Cell Biology,Chinese Academy of Sciences,Shanghai 200031,China ~2Department of Biology,Hong Kong University of Science and Technology,ChinaDr.Xing Wang Wang earned Ph.D.from Shanghai Institute of Materia Medical,Chinese Academy of Sciences in 1997.Now a professor at Shanghai Institute of Cell Biology,Chinese Academy of Sciences. | 2001 | World Journal of Gastroenterology2001，7，3： | 21 |
| 2 | Growth-inhibiting effects of taxol on human liver cancer in vitro and in nude mice显示文摘AIM To investigate the effects of taxol onSMMC-7721 human hepatoma and itsmechanisms.METHODS In vitro cell growth was assessedby trypan blue exclusion method.Experimentalhepatoma model was established by seedingSMMC-7721 cells subcutaneously into Balb/c(nu/nu)nude mice.In vivo tumor growth wasdetermined by measurement of tumor diameterwith Vernier calipers.The syntheses of DNA,RNA and protein were analyzed by incorporationof ~3H-thymidine,~3H-uridine and ~3H-leucinerespectively.Using light and electronmicroscopes to observe the morphologicalchanges of cells including mitosis andapoptosis.RESULTS Taxol was effective against SMMC-7721 human hepatoma cell growth in the rangesof 2.5 nmol/L-10 nmol/L with mitotic arrestand apoptosis in vitro.DNA,RNA and proteinsyntheses in cells were also obviouslysuppressed by in vitro treatment of taxol for72 h.Taxol at 2.5 nmol/L reduced ~3H-thymidineuptake to about 34% of the control value(P<0.05).Increasing the dose of taxol to20 nmol/L resulted in a greater decrease in ~3H-thymidine incorporation to 60% of the controlvalue(P<0.01).At a concentration of 20 nmol/L,the ~3H-uridine and ~3H-leucine uptakeswere reduced to 52%(P<0.05)and 63%(P<0.01),respectively.In vivo,taxolsignificantly inhibited SMMC-7721 tumor growthat 10 mg/kg,i.p.,once daily for 10 d.A morethan 90% decrease in tumor volume wasobserved by day 11(P<0.01)similarly withmitotic arrest and cell apoptosis.CONCLUSION Taxol has a marked anticanceractivity in SMMC-7721 human hepatoma both invitro and in nude mice.Its mechanisms might beassociated with mitotic arrest,subsequently,apoptosis of the hepatoma cells.No obvioustoxicity was observed with in vivoadministration of taxoi. | Jin Hui Yuan Ru Ping Zhang Ru Gang Zhang Li Xia Guo Xing Wang Wang Dan Luo Yong Xie Hong Xie | 2000 | World Journal of Gastroenterology2000，6，2： | 18 |
| 3 | Susceptibility to hepatocellular carcinoma associated with null genotypes of GSTM] and GSTT1显示文摘AIM In order to study the association betweenthe null genotypes of GSTM1 and GSTT1 and thegenetic susceptibility to hepatocellularcarcinoma(HCC).METHODS The genotypes of GSTM1 and GSTT1of 63 cases of HCC and 88 controls were detectedwith the multiple PCR technique.RESULTS The frequency of GSTM1 nullgenotype was 57.1% among the cases,and42.0% among the controls,the difference beingstatistically significant(χ~2=3.35,P=0.067),but χ~2 value approaching the significance level.The odds ratio was 1.84(95% Cl= 0.91-3.37).The frequency of GSTT1 non-null genotype was87.3% among the cases and 52.5% among thecontrols,the difference being statisticallysignificant(χ~2=11.42,P=0.0007274).The oddsratio was 4.13(95% Cl=1.64-10.70).According to the cross analysis,the GSTT1 non-null genotype was more closely associated withHCC than GSTM1 null genotype,and these twofactors play an approximate additive interactionin the occurrence of HCC.CONCLUSION The persons with GSTM1 nullgenotype and GSTT1 non-null genotype have theincreased risk to HCC. | Jian Chao Bian Fu Min Shen Li Shen Tian Ru Wang Xiao Hong Wang Gong Chao Chen Jin Bing Wang Department of Epidemiology,Shanghai Medical University,Shanghai 200032,China Haimen Municipal Anti-Epidemic and Health Station,Haimen 226201,Jiangsu Province,China Qidong Institute for Liver Cancer,Qidong 226200,Jiangsu Province,China | 2000 | World Journal of Gastroenterology2000，6，2： | 12 |
| 4 | Gut microbiota dysbiosis-induced activation of the intrarenal renin-angiotensin system is involved in kidney injuries in rat diabetic nephropathy显示文摘Some studies have shown that gut microbiota along with its metabolites is closely associated with diabetic mellitus(DM).ln this study we explored the relationship between gut microbiota and kidney injuries of early diabetic nephropathy(DN)and its underlying mechanisms.Male SD rats were intraperitoneally injected with streptozotocin to induce DM.DM rats were orally administered compound broad-spectrum antibiotics for 8 wieeks.After the rats were sacrificed,their blood,urine,feces,and renal tssues were harvested for analyses.We found that compared with the control rats,DM rats had abnormal intestinalmicroflora,increased plasma acetate levels,increased proteinuria,thickened glomerular basement membrane,and podocyte foot process effacement in the kidneys.Furthermore,the protein levels of angiotensinⅡ,angiotensin-converting enzyme,and angiotensinⅡtype 1 receptor in the kidneys of DM rats were significantly increased.Administration of broad-spectrum antbiotics in DM rats not only completely killed most intestinal microflora,but also significanty lowered the plasma acetate levels,inhibited intrarenal RAS activation,and attenuated kidney damage.Finally,we showed that plasma acetate levels were positively correlated with intrarenal angiotensinⅡprotein expression(r=0.969,P<0.001).In conclusion,excessive acetate produced by disturbed gut microbiota might be involved in the kidney injuries of early DN through activating intrarenal RAS. | Chen-chen Lu Ze-bo Hu Ru Wang Ze-hui Hong Jian Lu Pei-pei Chen Jia-xiu Zhang Xue-qi Li Ben-yin Yuan Si-jia Huang Xiong-zhong Ruan Bi-cheng Liu Kun-ling Ma | 2020 | Acta Pharmacologica Sinica2020，41，8： | 12 |
| 5 | Effect of heparin on apoptosis in human nasopharyngeal carcinoma CNE2 cells显示文摘In order to study the mechanism of the effect of heparin on apoptosis in carcinoma cells, the nasopharyngeal carcinoma cell line CNE2 was used to identify the effect of heparin on apoptosis associated with the expression of c-myc, bax, bcl-2 proteins by use of Hoechst 33258 staining, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL), agarose gel electrophoresis, and flow cytometry, as well as Western blot analysis. The results showed that heparin induced apoptosis of CNE2 cells including the morphologic changes such as reduction in the volume, and the nuclear chromatin condensation, as well as the “ladder pattern” revealed by agarose gel electrophoresis of DNA in a concentration-dependent manner.The number of TUNEL-positive cells was dramatically increased to 33.6±1.2% from 2.8±0.3% by treat ment with heparin in different concentrations (10~40 kU/L). The apoptotic index was increased to 32.5% from 3.5% by detecting SubG1 peaks on flow cytometry. Western blot analysis showed that levels of bcl-2,bax and c-myc were significantly overexpressed by treatment with the increase of heparin concentrations.These results suggest that heparin induces apoptosis of CNE2 cells, which may be regulated by differential expression of apoptosis-related genes. | LI HONG LIANG , KAI HE YE , HAI WEI ZHANG , YING RU LUO , XIAN DA REN, AI HUA XIONG, RUI SITU Department of Pharmacology ,Pharmacy College, Department of Pathology Medical College, Jinan University, Guangzhou 510632, China | 2001 | Cell Research2001，11，4： | 9 |
| 6 | Using a non-radioisotopic, quantitative TRAP-based method detecting telomerase activities in human hepatoma cells显示文摘A non-radioisotopic, quantitative TRAP-based telomerase activity assay was established mainly by using SYBR Green-I staining instead of radioisotope. Comparing with conventional radioisotope based method, it was better in reproducibility and accuracy. Using this method, we found telomerase activities were absent in normal human liver cells, while detected in ail of four human hepatoma cell lines (BEL-7404, SMMC-7721, QGY-7903 and HCCM) without significant differences. | ZHANG RU GANG XING WANG WANG JIN HUI YUAN LI XIA GUO HONG XIE (Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China) | 2000 | Cell Research2000，10，1： | 8 |
| 7 | Elevated Resting Heart Rate is Associated with Dyslipidemia in Middle-aged and Elderly Chinese显示文摘Objective To study the relationship between resting heart rate and blood lipid level.Methods A total of 9 415 subjects aged≥40 years were included in the present study.Their resting heart rate was monitored and their serum levels of triglyceride(TG),total cholesterol(TC),low density lipoprotein cholesterol(LDL-C)and high density lipoprotein cholesterol(HDL-C)were measured to define dyslipidemia according to the 2007 Chinese Guidelines on Prevention and Treatment of Dyslipidemia in Adults.Results The subjects were divided into group A with their resting heart rate<70 beats/min,group B with their resting heart rate=70-79 beats/min,group C with their resting heart rate=80-89 beats/min,and group D with their resting heart rate≥90 beats/min.High TG,TC,and LDL-C were presented across the resting heart rate(Ptrend<0.01).Multiple logistic regression analysis revealed that the risk of high TG and TC was higher in subjects with their resting heart rate≥90 beats/min than in those with their resting heart rate<70 beats/min(OR=1.42;95%CI:1.16-1.74 and OR=1.33;95%CI:1.09-1.64,respectively).Conclusion Elevated resting heart rate is associated with high TG and TC in middle-aged and elderly Chinese subjects. | SUN Ji Chao HUANG Xiao Lin DENG Xin Ru LV Xiao Fei LU Jie Li CHEN Yu Hong BI Yu Fang WANG Wei Qing XU Min NING Guang | 2014 | Biomedical and Environmental Sciences2014，27，8： | 5 |
| 8 | Regular Space-like Hypersurfaces in S1^m+1 with Parallel Para-Blaschke Tensors显示文摘In this paper, we give a complete conformal classification of the regular space-like hypersurfaces in the de Sitter Space S_1^(m+1) with parallel para-Blaschke tensors. | Xing Xiao LI Hong Ru SONG | 2017 | Acta Mathematica Sinica,English Series2017，33，10： | 5 |
| 9 | Simultaneous determination of metronidazole and tinidazole in plasma by using HPLC-DAD coupled with second-order calibration显示文摘A method using HPLC-DAD coupled with second-order calibration was developed to simultaneously determine metronidazole and tinidazole in plasma samples in this paper.The second-order calibration method based on APTLD(alternating penalty trilinear decomposition) algorithm was proposed to analyze the three-way HPLC-DAD data from both standard and prediction samples, which makes it possible that calibration can be performed even in the presence of unknown interferences with a simple and green chromatographic condition and short analysis time.The results showed that good recoveries were obtained although the chromatographic and spectral profiles of the analytes of interest as well as background were partially overlapped with each other in plasma samples. | Li Qun Ouyang Hai Long Wu Ya Juan Liu Jian Yue Wang Yong Jie Yu Hong Yan Zou Ru Qin Yu | 2010 | Chinese Chemical Letters2010，21，10： | 4 |
| 10 | Inhibitory effects of lapachol on rat C6 glioma in vitro and in vivo by targeting DNA topoisomerase Ⅰ and topoisomerase Ⅱ显示文摘OBJECTIVE The aim of this study is to investigate the inhibitory effects of lapachol on rat C6 glioma both in vitro and in vivo,as well as the potential mechanisms.METHODS First,the model of C6 glioma in Wistar rats was established and verified by hemotoxylin and eosin staining,immunohistochemical staining and magnetic resonance imaging(MRI).Then different doses of lapachol were gavaged and tumor volumes of the C6 glioma were detected by MRI.The effects of lapachol on C6 cell proliferation,apoptosis and DNA damage were detected by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium(MTS)/phen-azinemethosulfate(PMS)assay,Hoechst33358 staining,AnnexinⅤ-FITC/PI staining,and comet assay.Effects of lapachol on topoisomeraseⅠ(TOPⅠ)and topoisomeraseⅡ(TOPⅡ)activities were detected by TOPⅠand TOPⅡmediated supercoiled p BR322 DNA relaxation assay.Molecular docking was used to predict the interaction of lapachol-TOPⅠand lapachol-TOPⅡ.TOP I and TOPⅡexpression levels in C6 cells were determined by Enzymelinked immunosorbent assay kits and real-time polymerase chain reaction(RT-PCR).RESULTS The rat C6 glioma model was successfully established.High dose lapachol showed significant inhibitory effect on the C6 glioma in Wistar rats(P<0.05).MTS/PMS assay,Hoechst 33258 staining,AnnexinⅤ-FITC/PI staining,and comet assay showed that lapachol could inhibit proliferation,induce apoptosis and DNA damage of C6 cells in dose dependent manners.Lapachol could inhibit the activities of both TOPⅠandⅡ.Molecular docking showed that lapachol-TOPⅠshowed relatively stronger interaction than that of lapachol-TOPⅡ.Enzyme-linked immunosorbent assay and RT-PCR showed that lapachol could inhibit TOPⅡexpression levels,but not TOPⅠexpression levels.CONCLUSION These results showed that lapachol could significantly inhibit C6 glioma both in vivo and in vitro,which might be related with inhibiting TOPⅠand TOPⅡactivities,as wel as TOPⅡexpression. | XU Huan-li CHEN Qun-ying WANG Hong XU Ping-xiang YUAN Ru LI Xiao-rong BAI Lu XUE Ming | 2016 | 中国药理学与毒理学杂志2016，30，10： | 3 |
| 11 | Preparation of polymer-supported phosphine from ferrocene for palladium-catalyzed Suzuki-Miyaura cross-coupling reactions显示文摘Polymer-supported phosphine ligand 3b derived from ferrocene was prepared,and applied in palladium-catalyzed Suzuki- Miyaura reactions.A range of aryl bromides can couple with phenylboronic acid to obtain corresponding biaryls in excellent yields. The recycling of the polymer-bound catalyst was tested without adding palladium. | Hong Wei Yu Qing Song Tong Yi Ru Peng Li Jia Ji Cheng Shi Zi Lin Jin | 2007 | Chinese Chemical Letters2007，18，1： | 2 |
| 12 | Serum Calcium Level is Associated with Brachial-ankle Pulse Wave Velocity in Middle-aged and Elderly Chinese显示文摘Objective To study the relation between serum calcium level and elevated BaPWV in Chinese subjects.Methods The relation between serum calcium level and elevated BaPWV was studied in 9 615subjects.The mean value of left and right BaPWV was analyzed.BaPWV was defined as high when it was?1 752.5 cm/s(the upper quartile)either side.Results The BaPWV and its elevated percentage progressively increased across the quartiles of the serum calcium level(P<0.05).The prevalence of elevated BaPWV was significantly higher in subjects of the second,third and highest quartiles than in those of the lowest quartile(26.9%,28.4%,and 33.2%vs23.7%,P=0.0116,P=0.0004,and P<0.0001).Logistic regression analysis revealed that the risk of elevated BaPWV was 1.32-fold higher in subjects of the highest quartile than in those of the lowest quartile(OR=1.32,95%CI:1.08-1.60).Conclusion The elevated serum calcium level is related to an elevated BaPWV and a higher risk of arterial stiffness,independent of conventional risk factors,in middle-aged and elderly Chinese subjects. | DENG Xin Ru ZHANG Yin Fei WANG Tian Ge XU Bai Hui SUN Ji Chao ZHAO Lie Bin XU Min CHEN Yu Hong WANG Wei Qing BI Yu Fang LU Jie Li | 2014 | Biomedical and Environmental Sciences2014，27，8： | 2 |
| 13 | Inverted U-Shaped Associations between Glycemic Indices and Serum Uric Acid Levels in the General Chinese Population:Findings from the China Cardiometabolic Disease and Cancer Cohort(4C)Study显示文摘Objective The relationship between serum uric acid(SUA)levels and glycemic indices,including plasma glucose(FPG),2-hour postload glucose(2 h-PG),and glycated hemoglobin(HbA1 c),remains inconclusive.We aimed to explore the associations between glycemic indices and SUA levels in the general Chinese population.Methods The current study was a cross-sectional analysis using the first follow-up survey data from The China Cardiometabolic Disease and Cancer Cohort Study.A total of 105,922 community-dwelling adults aged≥40 years underwent the oral glucose tolerance test and uric acid assessment.The nonlinear relationships between glycemic indices and SUA levels were explored using generalized additive models.Results A total of 30,941 men and 62,361 women were eligible for the current analysis.Generalized additive models verified the inverted U-shaped association between glycemic indices and SUA levels,but with different inflection points in men and women.The thresholds for FPG,2 h-PG,and HbA1 c for men and women were 6.5/8.0 mmol/L,11.0/14.0 mmol/L,and 6.1/6.5,respectively(SUA levels increased with increasing glycemic indices before the inflection points and then eventually decreased with further increases in the glycemic indices).Conclusion An inverted U-shaped association was observed between major glycemic indices and uric acid levels in both sexes,while the inflection points were reached earlier in men than in women. | ZHU Yuan Yue ZHENG Rui Zhi WANG Gui Xia CHEN Li SHI Li Xin SU Qing XU Min XU Yu CHEN Yu Hong YU Xue Feng YAN Li WANG Tian Ge ZHAO Zhi Yun QIN Gui Jun WAN Qin CHEN Gang GAO Zheng Nan SHEN Fei Xia LUO Zuo Jie QIN Ying Fen HUO Ya Nan LI Qiang YE Zhen ZHANG Yin Fei LIU Chao WANG You Min WU Sheng Li YANG Tao DENG Hua Cong ZHAO Jia Jun CHEN Lu Lu MU Yi Ming TANG Xu Lei HU Ru Ying WANG Wei Qing NING Guang LI Mian LU Jie Li BI Yu Fang | 2021 | Biomedical and Environmental Sciences2021，34，1： | 2 |
| 14 | An Encapsulation of the Rare Earth Complex in Modified Mesoporous Molecular Sieve MCM-41显示文摘The encapsulation of a rare earth (RE) complex Eu(DBM)3phen in modified Si- MCM41 with 3 -aminopropyltriethoxy st.lane is reported for the first time. The luminescence intensity of the RE complex in the modified Si-MCM-41 is about 9 times as strong as in unmodified Si- MCM41 and the luminescence of the RE complex in the modified Si-MCM-41 has good color purity. | Qing Hong XU Lian She FU Lian Sheng LI Hong Jie ZHANG Qing Quo MENG Ru Ren XU Key Laboratory of Inorganic Synthesis & Preparative Chemistry, Department of Chemistry, Jilin University, Changchun 130023 Key Laboratory of Rare Earth Chemistry & Physic | 2000 | Chinese Chemical Letters2000，11，9： | 1 |
| 15 | Isolation and characterization of a marine algicidal bacterium against the toxic dinoflagellate Alexandrium tamarense显示文摘 | Jian Qiang Su Xiao Ru Yang Tian Ling Zheng Yun Tian Nian Zhi Jiao Li Zhe Cai Hua Sheng Hong | 2007 | Harmful Algae2007，，6： | 1 |
| 16 | Excited state intramolecular proton transfer of novel conjugated derivatives containing hydroxy and imino groups显示文摘This letter presents excited state intramolecular proton transfer(ESIPT) of new conjugated derivatives containing hydroxy and imino groups.ESIPT occurrence in one photon process is confirmed by well-separated emission band for the derivatives.Twophoton absorption(TPA) induced ESIPT emission has been determined by Ti:sapphire femtosecond laser tuning from 700 nm to 800 nm at the internals of 20 nm. | Xiao Lin Zhong Fang Gao Qi Wang Hong Ru Li Sheng Tao Zhang | 2010 | Chinese Chemical Letters2010，21，10： | 1 |
| 17 | Influence of Iron Supplementation on DMT1(IRE)-induced Transport of Lead by Brain Barrier Systems in vivo显示文摘Objective To investigate the potential involvement of DMT1(IRE) protein in the brain vascular system in vivo during Pb exposure. Methods Three groups of male Sprague-Dawley rats were exposed to Pb in drinking water, among which two groups were concurrently administered by oral gavage once every other day as the low and high Fe treatment group, respectively, for 6 weeks. At the same time, the group only supplied with high Fe was also set as a reference. The animals were decapitated, then brain capillary-rich fraction was isolate from cerebral cortex. Western blot method was used to identify protein expression, and RT-PCR to detect the change of the m RNA. Results Pb exposure significantly increased Pb concentrations in cerebral cortex. Low Fe dose significantly reduced the cortex Pb levels, However, high Fe dose increased the cortex Pb levels. Interestingly, changes of DMT1(IRE) protein in brain capillary-rich fraction were highly related to the Pb level, but those of DMT1(IRE) m RNA were not significantly different. Moreover, the consistent changes in the levels of p-ERK1/2 or IRP1 with the changes in the levels of DMT1(IRE). Conclusion These results suggest that Pb is transported into the brain through DMT1(IRE), and the ERK MAPK pathway is involved in DMT1(IRE)-mediated transport regulation in brain vascular system in vivo. | AN Dai Zhi AI Jun Tao FANG Hong Juan SUN Ru Bao SHI Yun WANG Li Li WANG Qiang | 2015 | Biomedical and Environmental Sciences2015，28，9： | 1 |
| 18 | FEM for Brake Discs of SiC 3D Continuous Ceramic Reinforced 7075 Aluminum Alloy for CRH3 Trains Applying Emergency Braking显示文摘 | Yu Liang Jiang Yan Li Lu Sen Kai Ru Hong Qiang Fang Ming | 2012 | Applied Mechanics and Materials2012，，120： | 1 |
| 19 | Microstructures of Co-Continuous SiC/Fe-2Cr13 Composite Fabricated by Vacuum-Pressure Casting and Infiltration Processes显示文摘 | Yu Liang Jiang Yan Li Ru Hong Qiang Liu Jiang Tao Luo Kun | 2011 | Advanced Materials Research2011，，239： | 1 |
| 20 | The update of prostatic ductal adenocarcinoma显示文摘Since initially described in 1967, prostatic ductal adenocarcinoma(PDA) has engendered a series of controversies on its origin, histological features, and biological behavior. Owing to the improvement of molecular biological technique, there are some updated findings on the characteristics of PDA. In the current review, we will mainly analyze its origin, clinical manifestations, morphological features, differential diagnosis, immunophenotype and molecular genetics, with the purpose of enhancing recognition of this tumor and making a correct diagnosis and treatment choice. | Tantan Liu Yingmei Wang Ru Zhou Haiyang Li Hong Cheng Jing Zhang | 2016 | Chinese Journal of Cancer Research2016，28，1： | 1 |
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