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| 1 | Sustained high level transgene expression in mammalian cells mediated by the optimized piggyBac transposon system显示文摘Sustained,high level transgene expression in mammalian cells is desired in many cases for studying gene functions.Traditionally,stable transgene expression has been accomplished by using retroviral or lentiviral vectors.However,such viral vector-mediated transgene expression is often at low levels and can be reduced over time due to low copy numbers and/or chromatin remodeling repression.The piggyBac transposon has emerged as a promising nonviral vector system for efficient gene transfer into mammalian cells.Despite its inherent advantages over lentiviral and retroviral systems,piggyBac system has not been widely used,at least in part due to their limited manipulation flexibilities.Here,we seek to optimize piggyBac-mediated transgene expression and generate a more efficient,user-friendly piggyBac system.By engineering a panel of versatile piggyBac vectors and constructing recombinant adenoviruses expressing piggyBac transposase(PBase),we demonstrate that adenovirusmediated PBase expression significantly enhances the integration efficiency and expression level of transgenes in mesenchymal stem cells and osteosarcoma cells,compared to that obtained from co-transfection of the CMV-PBase plasmid.We further determine the drug selection timeline to achieve optimal stable transgene expression.Moreover,we demonstrate that the transgene copy number of piggyBac-mediated integration is approximately 10 times higher than that mediated by retroviral vectors.Using the engineered tandem expression vector,we show that three transgenes can be simultaneously expressed in a single vector with high efficiency.Thus,these results strongly suggest that the optimized piggyBac system is a valuable tool for making stable cell lines with sustained,high transgene expression. | Xiang Chen Jing Cui Zhengjian Yan Hongmei Zhang Xian Chen Ning Wang Palak Shah Fang Deng Chen Zhao Nisha Geng Melissa Li Sahitya K.Denduluri Rex C.Haydon Hue H.Luu Russell R.Reid Tong-Chuan He | 2015 | Genes & Diseases2015，2，1： | 5 |
| 2 | Multicenter phaseⅡstudy of apatinib single or combination therapy in HER2-negative breast cancer involving chest wall metastasis显示文摘Objective:Breast cancer(BC)with chest wall metastasis(CWM)usually shows rich neovascularization.This trial explored the clinical effect of apatinib on human epidermal growth factor receptor 2(HER2)-negative advanced BC involving CWM.Methods:This trial involved four centers in China and was conducted from September 2016 to March 2020.Patients received apatinib 500 mg/d[either alone or with endocrine therapy if hormone receptor-positive(HR+)]until disease progression or unacceptable toxicity.Progression-free survival(PFS)was the primary endpoint.Results:We evaluated 26 patients for efficacy.The median PFS(mPFS)and median overall survival(mOS)were4.9[range:2.0-28.5;95%confidence interval(95%CI):2.1-8.3]months and 18(range:3-55;95%CI:12.9-23.1)months,respectively.The objective response rate(ORR)was 42.3%(11/26),and the disease-control rate was76.9%(20/26).In the subgroup analysis,HR+patients compared with HR-negative patients had significantly improved mPFS of 7.0(95%CI:2.2-11.8)months vs.2.3(95%CI:1.2-3.4)months,respectively(P=0.001);and mPFS in patients without or with chest wall radiotherapy was 6.4(95%CI:1.6-19.5)months vs.3.0(95%CI:1.3-4.6)months,respectively(P=0.041).In the multivariate analysis,HR+status was the only independent predictive factor for favorable PFS(P=0.014).Conclusions:Apatinib was highly effective for BC patients with CWM,especially when combined with endocrine therapy.PFS improved significantly in patients with HR+status who did not receive chest wall radiotherapy.However,adverse events were serious and should be carefully monitored from the beginning of apatinib treatment. | Huiping Li Cuizhi Geng Hongmei Zhao Hanfang Jiang Guohong Song Jiayang Zhang Yaxin Liu Xinyu Gui Jing Wang Kun Li Zhongsheng Tong Fangyuan Zhao Junlan Yang Guoliang Chen Qianyu Liu Xu Liang | 2021 | Chinese Journal of Cancer Research2021，33，2： | 4 |
| 3 | Four-year dynamic observation and study on standardized management of elderly patients with type 2 diabetes in Beijing Yongding Road Community显示文摘Purpose:This study aimed to understand the joint standard of blood glucose,blood li-pids,and blood pressure in elderly patients>60 years of age with type 2 diabetes in the Beijing Yongding Road Community.Methods:One hundred ninety-two elderly patients>60 years of age who were diagnosed with type 2 diabetes in Yongding Road Community served as the investigation subjects,under-went standard management,and the dynamic changes in blood glucose,blood lipids,and blood pressure were monitored for 48 months.Results:At the end of the observation period,the standard rates of fasting blood glucose and postprandial blood glucose were 72.55%and 80.00%,respectively,which were increased compared with 55.73%and 56.08%at baseline(P<0.01).The standard rate of HbA1c was 59.81%,which was increased compared with 53.44%at baseline(P>0.05).The standard rates of TG and LDL-C were 76.71%and 60.38%,respectively,which were increased compared with 54.69%and 34.74%at base-line,and the standard rate of HDL-C was 13.64%,which was decreased compared with 40.10%at baseline(P<0.01).The standard rate of BP was 58.33%,which was increased compared with 38.54%at baseline(P<0.01).The optimal control rate of blood glucose for 48 months(the standard times of the total measurement times in 48 months≥75%)was higher;the fasting blood glucose was 52.17%,the 2-h postprandial blood glucose was 60.22%,and the HbA1c was 46.45%.The optimal control rate of blood lipids was lower;LDL-C was 17.49%and HDL-C was 13.59%.The optimal control rate of BP was 9.13%.At the end of the observation period,the levels of fasting blood glucose and postprandial blood glucose were decreased by 0.7 mmol/L and 1.48 mmol/L,respectively,compared with the base-line(P<0.01).The level of HbA1c was decreased by 0.18%compared with the baseline(P<0.05).The levels of LDL-C and HDL-C were decreased by 0.4 mmol/L and 0.23 mmol/L,respectively,compared with the baseline(P<0.01).The levels of SBP and DBP were decreased by 4 mmHg compared with the baseline(P<0.01).At the end of the observation period,the joint standard rate of the three indices of HbA1c,LDL-C,and BP was 24.72%,which was increased compared with 6.25%at baseline(P<0.01).Conclusion:Standardized management of elderly patients with diabetes in the Community can improve the joint standard rate of blood glucose,blood lipids,and blood pressure.The op-timal control rate and joint standard rate are the important indices for evaluating the quality of diabetes management. | Yue Li Dongyun Shen Wei Zhang Yimin Wang Hongmei Li Jie Geng Liying Yang Zhijun Xiao Yan Liu Zhigang Feng Linying Cao Yan Jin Lixia Shi | 2014 | Family Medicine and Community Health2014，2，2： | 4 |
| 4 | Simultane-ous HPLC determination of five flavonoids in Flos Inulae显示文摘 | Geng Hongmei Zhang Diqun Zha Jianpeng | 2007 | Chromatographia2007，66，34： | 1 |
| 5 | Simultaneous HPLC Determination of Five Flavonoids in Flos Inulae显示文摘 | Hongmei Geng Diqun Zhang Jianpeng Zha | 2007 | Chromatographia2007，66，3： | 1 |
| 6 | Hydrogen sulfide ameliorates senescence in vascular endothelial cells through ameliorating inflammation and activating PPARδ/SGLT2/STAT3 signaling pathway显示文摘Mounting evidence demonstrates that hydrogen sulfide(H2S)promotes anti-inflammatory molecules and inhibits pro-inflammatory cytokines in endothelial cells(ECs).This study aims to investigate the favorable action of H2S on endothelial function in senescence by inhibiting the production of inflammatory molecules.Senescent ECs exhibit a reduction in H2S,endothelial nitric oxide synthase(eNOS)and peroxisome proliferator-activated receptorδ(PPARδ),coupled with increased inflammatory molecules,sodium glucose transporter type 2(SGLT2)and phosphorylation of STAT3,which could be reversed by the administration of a slow but sustained release agent of H2S,GYY4137.Decreased production of eNOS and upregulated p-STAT3 and SGLT2 levels in senescent ECs are reversed by replenishment of the SGLT2 inhibitor EMPA and the PPARδagonist GW501516.The PPARδantagonist GSK0660 attenuates eNOS expression and increases the production of p-STAT3 and SGLT2.However,supplementation with GYY4137 has no beneficial effect on GSK0660-treated ECs.GYY4137,GW501516 and EMPA preserve endothelialdependent relaxation(EDR)in D-gal-treated aortae,while GSK0660 destroys aortic relaxation even with GYY4137 supplementation.In summary,senescent ECs manifest aggravated the expressions of the inflammatory molecules SGLT2 and p-STAT3 and decreased the productions of PPARδ,eNOS and CSE.H2S ameliorates endothelial dysfunction through the anti-inflammatory effect of the PPARδ/SGLT2/p-STAT3 signaling pathway in senescent ECs and may be a potential therapeutic target for anti-ageing treatment. | Danyang Tian Jinqi Meng Lin Li Hongmei Xue Qi Geng Yuxin Miao Meng Xu Ru Wang Xiangjian Zhang Yuming Wu | 2023 | Acta Biochimica et Biophysica Sinica2023，55，9： | 1 |
| 7 | Monitoring of Avian Influenza Virus and Newcastle Disease Virus in Suichuan Region of Jiangxi Province显示文摘[Objective] The research aimed to investigate the epidemiological characteristics of Avian influenza virus( AIV) and Newcastle disease virus( NDV)of wild Ardeid birds in Suichuan region of Jiangxi Province. [Method] A total of 110 nasopharyngeal swabs and 110 cloacal swabs of Ardeid birds were collected from Suichuan region of Jiangxi Province in October of 2014. The swabs were conducted the virus isolation using SPF chicken embryos,suspected positive samples were screened by hemagglutination( HA) test and identified by Reverse Transcription-Polymerase Chain Reaction( RT-PCR). [Results] HA titre of all 110 test samples was 0 except four samples. Detection on AIV and NDV of the test samples by RT-PCR showed that all the samples were negative,which indicated that the infection risk of avian influenza( AI) and newcastle disease( ND) of Ardeid birds in Suichuan region in Jiangxi Province in the summer of 2014 was low. [Conclusion] The research provided the basic data for studying the infection situations of AI and ND of wild waterfowl in Jiangxi Province. | Geng Haidong Jin Hongmei Zhang Yanjun Zhang Xiaolin Zhang Jiajie Li Xiang Zhou Changyu Ding Yuanying Gao Guangyu Wang Zhuole Qiao Dan Li Yinxue Zhai Xiwen Hua Yuping Zhang Guogang Chai Hongliang | 2016 | Animal Husbandry and Feed Science2016，8，4： | 0 |
| 8 | Study on Related Factors of Aspirin Resistance in Acute Ischemic Stroke显示文摘Objective:To study the related factors of aspirin resistance(AR)in acute ischemic stroke.Methods:A total of 138 patients with acute ischemic stroke treated in hospital affiliated to Xuzhou medical university from August 2016 to August 2018 were the study subjects,examine his medical data from the past.They were divided into the AR group(40 cases)and the non-AR group(98 cases)according to whether AR appears.Gender,disease history,biochemical indicators and etc.were compared between the two groups.The independent risk factors of AR were investigated using univariate analysis and logistic regression analysis.Results:40 cases of AR occurred in 138 patients,with an incidence rate of 28.99%.Diabetes,platelet count(PLT),microRNA-19a(m iR-19a)expression,smoking,high-sensitivity C-reactive protein(hs-CRP),Low-density lipoprotein cholesterol(LDL-C),fibrinogen(FIB)and age difference between the AR group and non-AR group was statistically significant(P<0.05).Gender,hypertension,uric acid(UA),high-density lipoprotein cholesterol(HDL-C),triglycerides(TG),homocysteine(Hcy),total cholesterol(TC),and alanine aminotransferase(ALT)between the two groups were not significantly different(P>0.05).Logistic regression analysis showed that the independent risk factors for AR in acute ischemic stroke were diabetes(OR=2.773,95%CI:1.102~5.065,P=0.025),miR-19a(OR=3.021,95%CI:1.322~6.545,P=0.021),hs-CRP(OR=2.719,95%CI:1.301~5.022,P=0.028)and smoking(OR=1.983,95%CI:1.114~3.887,P=0.040).Conclusion:The incidence of AR is higher in acute ischemic stroke.Risk factors include diabetes,miR-19a expression,hs-CRP,smoking,etc.Clinical intervention measures can be taken to reduce the risk of AR and improve acute ischemic stroke prognosis. | Yuxi Shi Hongmei Ding Deqin Geng | 2022 | Journal of Clinical and Nursing Research2022，6，1： | 0 |
| 9 | Solidification behavior of SiC_w/Al-18Si composites显示文摘SiCw/Al-18Si composites were prepared by squeeze casting technique.Solidification behavior of SiCw/Al-18Si composites was studied by differential scanning calorimeter(DSC) and scanning electron microscopy(SEM).The re-melting temperature has little effect on the solidification behavior and microstructure of composites.With the increase of SiC whisker content,onset and peak temperatures of primary Si decrease and the average size of primary silicon becomes smaller and smaller.Sr addition lowers onset and peak temperatures of primary Si,however,onset and peak temperatures of eutectic were barely affected by Sr addition.The average sizes of primary Si and Al-Si eutectic both decrease by Sr addition. | WEI Hongmei,GENG Lin,and ZHANG Xuexi School of Materials Science and Engineering,Harbin Institute of Technology,Harbin 150001,China | 2007 | Rare Metals2007，26，S1： | 0 |
| 10 | Enhancement of interfacial bond strength of Al_2O_(3f)/Al-15Si composite by solidification process显示文摘To build the relationship between solidification conditions and the interfacial bond strength,Al2O3f/Al-15Si composite solidified at different cooling rates were investigated by microstructure observation and mechanical testing.The results show that the interfacial bond strength of the as-cast composite is significantly enhanced after furnace-or air-cooling processes owning to the preferential nucleation and growth of the eutectic silicon on the fiber surface.The discontinuously distributed eutectic silicon on the fiber surface makes the fiber become coarse,resulting in the enhanced mechanical bond strength between fiber and matrix.However,the interfacial bond strength of the oil-cooled composite is not improved,showing that the silicon on the fiber surface is important for the improvement of the interfacial bond strength.The air-and furnace-cooled composites also have a higher tensile strength. | ZHANG Xuexi,WEI Hongmei,WANG Dezun,and GENG Lin School of Materials Science and Engineering,Harbin Institute of Technology,Harbin 150001,China | 2007 | Rare Metals2007，26，S1： | 0 |
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