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您的检索式：作者名="Hongyang Wan"
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| 1 | Delaminated layered double hydroxide delivers DNA molecules as sandwich nanostructure into cells via a non-endocytic pathway显示文摘Layered double hydroxides(LDHs) are effective molecular carriers in cytological research, gene therapy,and transgenic applications. Herein, we investigated the internalization behavior of the LDH-DNA bioconjugates via a microscopic approach and analyzed the internalization pathway by dissipative particle dynamics(DPD) simulations. We experimentally found that LDH can efficiently carry DNA into the nucleus of cell in BY-2 suspension cells. Furthermore, atomic force microscopy and X-ray diffraction analysis demonstrated that the LDH-DNA bioconjugates mainly exist as a DNA-LDH-DNA sandwich complex,while the LDH-DNA-LDH sandwich complex and DNA-LDH complex cannot be excluded. The DPD simulations further indicated that only the DNA-LDH-DNA sandwich structure could penetrate the plasma membrane(PM), while PM is impermeable to the LDH-DNA-LDH sandwich complex and the DNA-LDH complex. This work provides novel perspective for understanding the membrane penetration mechanism of LDH nano-sheets and new insights into the design of novel molecular delivery systems.ó 2017 Science China Press. Published by Elsevier B.V. and Science China Press. All rights reserved. | Ye Li Wenlong Bao Hongyang Wu Junya Wang Yu Zhang Yinglang Wan Dapeng Cao Dermot O'Hare Qiang Wang | 2017 | Science Bulletin2017，62，10： | 1 |
| 2 | Understanding auditory neuropathy spectrum disorder: a system- atic review in transgenic mouse models显示文摘Auditory neuropathy spectrum disorder is a unique group of hearing dysfunctions characterized by preserved outer hair cell function and abnormal neural conduction of the auditory pathway. However, the pathogenic mechanism underlying this disorder is not clear. We therefore performed a systematic review of genetic mouse models with different gene mutations to provide a valuable tool for better understanding of the process and the possible molecular mechanisms. Of the 18 articles retrieved, nine met the required criteria. All biochemical, histological, and electrophysiological results were recorded for each of the mouse models, as was the transgenic technology. This review provides a summary of different mouse models that may play an important role in the diagnosis and management of auditory neuropathy spectrum disorder in the future. | Li Wang Jing Guan Hongyang Wang Lan Lan Qiujing Zhang Liang Zong Wan Du Wenping Xiong Fengjiao Li Kaiwen Wu Dayong Wang Qiuju Wang | 2016 | Science China(Life Sciences)2016，59，5： | 1 |
| 3 | A VGGNet-based correction for satellite altimetry-derived gravity anomalies to improve the accuracy of bathymetry to depths of 6500 m显示文摘Understanding the topographic patterns of the seafloor is a very important part of understanding our planet.Although the science involved in bathymetric surveying has advanced much over the decades,less than 20%of the seafloor has been precisely modeled to date,and there is an urgent need to improve the accuracy and reduce the uncertainty of underwater survey data.In this study,we introduce a pretrained visual geometry group network(VGGNet)method based on deep learning.To apply this method,we input gravity anomaly data derived from ship measurements and satellite altimetry into the model and correct the latter,which has a larger spatial coverage,based on the former,which is considered the true value and is more accurate.After obtaining the corrected high-precision gravity model,it is inverted to the corresponding bathymetric model by applying the gravity-depth correlation.We choose four data pairs collected from different environments,i.e.,the Southern Ocean,Pacific Ocean,Atlantic Ocean and Caribbean Sea,to evaluate the topographic correction results of the model.The experiments show that the coefficient of determination(R~2)reaches 0.834 among the results of the four experimental groups,signifying a high correlation.The standard deviation and normalized root mean square error are also evaluated,and the accuracy of their performance improved by up to 24.2%compared with similar research done in recent years.The evaluation of the R^(2) values at different water depths shows that our model can achieve performance results above 0.90 at certain water depths and can also significantly improve results from mid-water depths when compared to previous research.Finally,the bathymetry corrected by our model is able to show an accuracy improvement level of more than 21%within 1%of the total water depths,which is sufficient to prove that the VGGNet-based method has the ability to perform a gravity-bathymetry correction and achieve outstanding results. | Xiaolun Chen Xiaowen Luo Ziyin Wu Xiaoming Qin Jihong Shang Huajun Xu Bin Li Mingwei Wang Hongyang Wan | 2024 | Acta Oceanologica Sinica2024，43，1： | 0 |
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