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| 1 | Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma显示文摘BACKGROUND:Hepatocellular carcinoma (HCC) is characterized by a multi-cause,multi-stage and multi-focus process of tumor progression.Its prognosis is poor and early diagnosis is of utmost importance.This study was undertaken to investigate the dynamic expression of oncofetal antigen glypican-3 (GPC-3) and GPC-3 mRNA in hepatocarcinogenesis and to explore their early diagnostic value for HCC.METHODS:A hepatoma model was induced in male Sprague-Dawley rats with 0.05% 2-fluorenylacetamide and confirmed by hematoxylin and eosin staining and gamma-glutamyltransferase (GGT) expression.Total RNA was purified and transcribed into cDNA by reverse transcription.Fragments of the GPC-3 gene were amplified by nested RT-PCR,and confirmed by sequencing.GPC-3 was analyzed by immunohistochemistry,Western blotting or ELISA.RESULTS:Positive GPC-3 expression showed as brown granule-like staining localized in the cytoplasm.Histological examination of hepatocytes revealed three morphological stages of granule-like degeneration,atypical hyperplasia (precancerous),and cancer formation,with a progressive increase of liver total RNA and GGT expression.The incidence of liver GPC-3 mRNA and GPC-3,and serum GPC-3 was 100%,100% and 77.8% in the HCC group,100%,100%,and 66.7% in the precancerous group,83.3%,83.3%,and 38.9% in the degeneration group,and no expression in the liver or blood of the control group,respectively.There was a positive correlation between liver GPC-3 mRNA and total RNA level (r=0.475,P<0.05) or liver GPC-3 (r=1.0,P<0.001) or serum GPC-3 (r= 0.994,P<0.001).CONCLUSION:Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of HCC. | Research Center of Clinical Medicine,Affiliated Hospital of Nantong University,Nantong 226001,China (Yao M,Yao DF,Qiu LW,Wu W,Sai WL,Yang JL and Zhang HJ) Department of Oncology,Yancheng First People’s Hospital,Yancheng 224001,China (Bian YZ) Department of Oncology,Second Affiliated Hospital,Nanjing Medical University,Nanjing 210011,China (Zhang CG) | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，3： | 51 |
| 2 | Subclinical abnormal glucose tolerance is a predictor of death in liver cirrhosis显示文摘AIM:To determine if subclinical abnormal glucose tolerance(SAGT)has influence on survival of non-diabetic patients with liver cirrhosis.METHODS:In total,100 patients with compensatedliver cirrhosis and normal fasting plasma glucose were included.Fasting plasma insulin(FPI)levels were measured,and oral glucose tolerance test(OGTT)was performed.According to OGTT results two groups of patients were formed:those with normal glucose tolerance(NGT)and those with SAGT.Patients were followed every three months.The mean follow-up was932 d(range of 180-1925).Survival was analyzed by the Kaplan-Meyer method,and predictive factors of death were analyzed using the Cox proportional hazard regression model.RESULTS:Of the included patients,30 showed NGT and70 SAGT.Groups were significantly different only in age,INR,FPI and HOMA2-IR.Patients with SAGT showed lower 5-year cumulated survival than NGT patients(31.7%vs 71.6%,P=0.02).Differences in survival were significant only after 3 years of follow-up.SAGT,Child-Pugh B,and high Child-Pugh and Model for EndStage Liver Disease(MELD)scores were independent predictors of death.The causes of death in 90.3%of cases were due to complications related to liver disease.CONCLUSION:SAGT was associated with lower survival.SAGT,Child-Pugh B,and high Child-Pugh and MELD scores were independent negative predictors of survival. | Diego García-Compeán Joel Omar Jáquez-Quintana Fernando Javier Lavalle-González José Alberto González-González Linda Elsa Mu?oz-Espinosa Jesús Zacarías Villarreal-PérezEndocrinology Service and Department of Internal Medicine University Hospital 'Dr. José E. González' and Medical School Universidad Autónoma de Nuevo León Monterrey 64320 México Héctor J Maldonado-Garza | 2014 | World Journal of Gastroenterology2014，20，22： | 12 |
| 3 | 镍钛形状记忆合金细胞相容性和生物力学兼容性的改进显示文摘镍钛记忆合金(NiTi)生物材料器件的发展,迫切要求研究和提高其细胞相容性和生物力学性能。依据美国材料测试标准协会(ASTM)的标准,我们研究表明:NiTi的细胞毒性比纯钛、Ti6A14V和316L不锈钢大,在NiTi表面作聚合四氟乙烯高离子(PPTFE)喷涂。可降低NiTi合金的细胞毒性,提高其抗腐蚀力。各种热处理方法可改善其疲劳性能,循环拉伸强度试验可测出其超弹性性能的衰减。 | L’H Yahia S.Lombardi N.Hagemeister G.Drouin C.H. Rivard M .Assad Okuyaman(Biomechanical-Biomaterials Research Group, Biomedical Engineering Institute, Ecole Polytechnique de Montreal,Canada)(Department of Orthopaedic Surgery,Hospital St.Justine, Montreal, | 1995 | 医用生物力学1995，10，2： | 9 |
| 4 | Interleukin-1α, 6 regulate the secretion of vascular endothelial growth factor A, C in pancreatic cancer显示文摘Vascular endothelial growth factor (VEGF, namely VEGF-A) is an angiogenic polypeptide and VEGF-C is a lymphangiogenic polypeptide that has been implicated in cancer growth, invasion and metastasis. Several cytokines and growth factors play an important part in cancer progression. These cytokines and growth factors are the principal mediators of cancer cells-stromal cell interaction , which is critical for invasion of cancer cells to the surrounding tissues and metastatic dissemination to distant organs. In this study, we studied VEGF-A, C expression in cultured human pancreatic cancer cell lines and whether the presence of VEGF-A, C in the cell lines is regulated by cytokines interleukin-lct (EL-1α), and interleukin-6 (IL-6). METHODS: We used Northern blot and Western blot methods to analyze expression of the gene and protein of VEGF-A, C in all 6 tested cell lines (ASPC-1, CAPAN-1, MIA-PaCa-2, PANC-1, COLO-357 and T3M4) respectively. To analyze what is the regulator for this VEGF-A, C expression in pancreatic cancer,we used the reverse transcription -polymerase chain reaction (RT-PCR) method to analyze VEGF-A, C expression in cultured human pancreatic cancer cell lines (CAPAN-1 and COLO-357) under the stimulation with IL-1α (10μg/L) or IL-6 (100 μg/L). RESULTS:Northern blot analysis revealed the presence of the 4.1-kb VEGF-A mRNA transcript and 2.4-kb VEGF-C mRNA transcript in all 6 tested cell lines. Immunoblotting with highly specific anti-VEGF-A, anti-VEGF-C antibody revealed the presence of a molecular weight of 43-kDa VEGF-A protein and 55-kDa VEGF-C protein in all the cell lines. RT-PCR analysis revealed the levels of the VEGF-A and VEGF-C gene were 1-2 fold and a 1-fold increase in the COLO-357 cell line by stimulation with IL-la, however, no effect was found in the CAPAN-1 cell line. The levels of the VEGF-A and VEGF-C gene were 2-5 fold and a 1-fold increase in the CAPAN-1 cell line by stimulation with IL-6, but, no effect was found in the COLO-357 cell line. CONCLUSION:These findings suggested that the expression of VEGF-A, C and their regulation by IL-1α, IL-6 in pancreatic cancer contributes to the lymphatic and distant metastasis and the disease progression. | Department of Hepatobiliary Surgery (Tang RF, Zhang FR, Peng L, Wang SX, Xiao Y and Zhang M) and Department of Dermatology (Wang SX), 4th Hospital, Hebei Medical University, Shijiazhuang 050011, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 6 |
| 5 | Relationship between alcohol consumption and clinical manifestation of patients with fatty liver:a single-center study显示文摘BACKGROUND:Fatty liver is a common chronic liver disease worldwide.It is associated with an increasing morbidity in China in recent years.The aim of this study was to analyze the effect of drinking alcohol on the hemoglobin and biochemical values of patients with fatty liver.METHODS:We investigated the clinical and laboratory data of 669 patients with fatty liver.Of the 669 patients,166 consumed alcohol more than 60 g per week for at least 2 years,and 503 did not have a history of long-term alcohol consumption.We further analyzed the relationship between alcohol consumption and clinical characteristics of these patients.RESULTS:The values of aspartate transaminase (AST),gammaglutamyl transpeptidase (GGT),and hemoglobin in the long-term consumption group were significantly higher than those in the non long-term consumption group (P<0.05).In the patients without long-term alcohol consumption,the values of GGT and hemoglobin in patients with light alcohol consumption were significantly higher than those in non alcohol consumers (P<0.05).CONCLUSION:Alcohol consumption is associated with significantly increased values of AST,GGT,and hemoglobin in patients with fatty liver,suggesting their potential roles in hepatic steatosis. | International Health Care Center (Wang XF) and Department of Gastroenterology (Yue M),First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310003,China | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，3： | 5 |
| 6 | Survival factors after resection of small hepatocellular carcinoma显示文摘Early resection of hepatocellular carcinoma is a key measure to prolong the survival of patients. This study was designed to summarize our experience in surgical resection of small hepatocellular carcinoma (HCC), and to analyze the factors influencing the postoperative survival of patients. METHODS:The clinicopathologic data of 105 patients with small HCC after resection from 1986 through 2003 were analyzed ; the patients had been followed up for more than half a year (median 33 months). Nine clinicopathologic factors, preoperative α-fetoprotein (AFP) level, liver cirrhosis, Child-Pugh score, tumor size (>2cm vs.≤2 cm) and number (single vs. multiple), capsule formation, portal vein tumor thrombi (PVTT), Edmondson tumor grade and surgical method, were analyzed by the log-rank test and the Cox proportional harzards model analysis. RESULTS:The cumulative 1-,3- and 5-year survival rates after the operation were 86.5%, 70.3% and 55.2%, respectively, and the 1-, 3- and 5-year disease-free survival rates were 78%, 58. 9% and 45. 6%, respectively. One patient died from esophagogastric varices hemorrhage in 2 weeks after reoperation. Thirty-six patients had intrahepatic recurrence or metastasis postoperatively and 34 patients died. The Kaplan-Meier method and the Cox proportional harzards model analysis indicated that poor Child-Pugh score, tumor more than 2 cm in diameter, PVTT and multiple lesions (including satellitic lesions) were adverse factors affecting postoperative survival. The Cox proportional harzards model analysis indicated that tumor size, PVTT and multiple lesions were the factors influencing postoperative disease-free survival. CONCLUSIONS:Limited hepatectomy with a margin more than 1 cm is an appropriate surgical approach. Adverse preoperative Child-Pugh score and postoperative intrahepatic recurrences are the main factors leading to the death of patients with small HCC. | Departments of Surgical Oncology (Wu FS, Zhao WH, Ma ZM, Teng LS and Wang M) and General Surgery ( Liang TB and Zheng SS) , First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 5 |
| 7 | Outcome in obscure gastrointestinal bleeding after capsule endoscopy显示文摘AIM: To investigate the clinical impact of capsule endoscopy(CE) after an obscure gastrointestinal bleeding(OGIB) episode, focusing on diagnostic work-up, followup and predictive factors of rebleeding. METHODS: Patients who were referred to Hospital del Mar(Barcelona, Spain) between 2007 and 2009 for OGIB who underwent a CE were retrospectively analyzed. Demographic data, current treatment with non-steroid antiinflammtory drugs or anticoagulant drugs, hemoglobin levels, transfusion requirements, previous diagnostic tests for the bleeding episode, as well as CE findings(significant or non-significant), work-up and patient out-comes were analyzed from electronic charts. Variables were compared by χ 2 analysis and Student t test. Risk factors of rebleeding were assessed by Log-rank test, Kaplan-Meier curves and Cox regression model. RESULTS: There were 105 patients [45.7% women, median age of 72 years old(interquartile range 56-79)] and a median follow-up of 326 d(interquartile range 123-641) included in this study. The overall diagnostic yield of CE was 58.1%(55.2% and 63.2%, for patients with occult OGIB and overt OGIB, respectively). In 73 patients(69.5%), OGIB was resolved. Multivariate analysis showed that hemoglobin levels lower than 8 g/dL at diagnosis [hazard ratios(HR) = 2.7, 95%CI: 1.9-6.3], patients aged 70 years and above(HR = 2.1, 95%CI: 1.2-6.1) and significant findings in CE(HR = 2.4, 95%CI: 1.1-5.8) were independent predictors of rebleeding. CONCLUSION: One third of the patients presented with rebleeding after CE; risk factors were hemoglobin levels < 8 g/dL, age ≥ 70 years or the presence of significant lesions. | Alex Caas-Ventura Lucia Márque Xavier Bessa Josep Maria DedeuDepartment of Gastroenterology Hospital del Mar Research Institute Pompeu Fabra University Marc Puigvehí Sílvia Delgado-Aros Ines Ana Ibáez Agustin Seoane Luis Barranco Felipe Bory Montserrat Andreu Begoa González-Suárez | 2013 | World Journal of Gastrointestinal Endoscopy2013，5，11： | 4 |
| 8 | Characterization of proteolipid protein-peptide-specific CD_4^+ T cell of experimental allergic encephalomyelitis in Biozzi AB/H mice | Yong Peng and Chih pin Liu Department of Neurology, The Third Affiliated Hospital, Hunan Medical University , Changsha 410013, China (Peng Y) Division of Immunology, Beckman Research Institute, City of Hope National M edical Center, Duarte, CA 9 | 2002 | Chinese Medical Journal2002，，4： | 3 |
| 9 | 直接评估吗啡刺激糖尿病人隐静脉内皮一氧化氮释放减少显示文摘to directly measure in real time basal and stimulated levels of NO released from human saphenous vein endothelium and to quantify the expression of the μopiate receptor, which has been linked with NO release. METHODS: Saphenous vein segments from patients with type 2 diabetes ( n = 12) and patients without diabetes (n =8) were obtained. The release of NO was measured directly from the endothelium using a NO-specific amperometric probe. NΩ-nitro-L-arginine methyl ester (L-NAME, 0.1 mmol/L), a NO synthase (NOS) inhibitor, or morphine (1 μmol/L), a stimulant, was administered and the measurements were repeated. Values were reported relative to the mean initial measurement of NO release from diabetic endothelium, which was defined as the relative zero level of NO release. A RT-PCR was then performed on the endothelium to measureμ opiate receptor expression. RESULTS: Diabetic patients (n = 12) showed a relative and significantly diminished basal level of released NO, (0.049± 0.012) nmol/L, compared | Thomas V BILHNGER, James A VOSSWINKEL, Patrick CADET, Christos M RIALAS, Harold I MAGAZINE, George B STEFANO(Division of Cardiothoracic Surgery, Department of Surgery, University Hospital and Medical Center, HSC, T19-080, Stony Brook, New York 11794-8191 Neuroscience Research Institute, State University of New York College at Old Westbury, Old Westbury, New York , USA) | 2002 | Acta Pharmacologica Sinica2002，23，2： | 3 |
| 10 | Postpartum hemolytic uremic syndrome:a rare entity and a treatment challenge 显示文摘 | Alsina Segui E Martin Conde M Craver Hospital L Fernandez Oiraldez E | 2008 | Nefrologia2008，28，： | 1 |
| 11 | Molecular diagnosis of infective endocarditis by re- al-time broad-range polymerase chain reaction (PCR) and sequencing directly from heart valve tissue 显示文摘 | Marin M Mufioz P Sdnchez M del Rosal M Alcal6 L Rodrlguez-Cr6ixems M Bouza E Group for the Manage- ment of Infective Endocarditis of the Gregorio Marafi6n Hospital | 2007 | Medicine2007，86，4： | 1 |
| 12 | Hospitalized patients with2009H1N1influenza in the United States,April-June2009显示文摘 | Jain S Kamimoto L Bramley AM Schmitz AM Benoit SR Louie J Sugerman DE Druckenmiller JK Ritger KA Chugh R Jasuja S Deutscher M Chen S Walker JD Duchin JS Lett S Soliva S Wells EV Swerdlow D Uyeki TM Fiore AE Olsen SJ Fry AM Bridges CB Finelli L 2009Pandemic Influenza A(H1N1)Virus Hospitalizations Investigation Team | 2009 | N Engl J Med2009，361，20： | 1 |
| 13 | Technological implications of reducing nitrate and nitrite levels indry-fermented sausages:Typical microbiota,residual nitrate and nitrite and volatile profile显示文摘 | HOSPITAL X F CARBALLO J FERNNDEZ M | 2015 | Food Control2015，57，： | 1 |
| 14 | New evidence of risk factors for community-acquired pneumonia: a population-based study 显示文摘 | Almirall J Bolibar I Serra-Prat M Roig J Hospital I Carandell E | 2008 | Eur Respir J2008，31，6： | 1 |
| 15 | Current burdenand probable increasing incidence of ESRD in China 显示文摘 | Zuo L Wang M Chinese Association of Blood PurificationManagement of Chinese Hospital Association | 2010 | ClinNephrol2010，74，1： | 1 |
| 16 | The use of molecular genetics in the improvement of agricultural populations 显示文摘 | Dekkers J C M Hospital F | 2002 | Nature Reviews Genetics2002，3，1： | 1 |
| 17 | Nutritional Status in Chronic Hemodialysis Patients显示文摘Hitherto, there has been no systematic evaluation of the nutritional status of HD patients in Thailand. 27 patients awaiting renal transplantation, 19 M 8 F, age 47.2 ± 8.6y (mean±SD), underwent HD at Ramathibodi Hospital. Dietary assessment revealed the average daily intake of men and women to be respectively 1571 ±314 and 1379 ± 403 kcal, 47 ± 11 and 42 ± 14 g protein. Anthropometric data showed that mean of BMI,percent ideal body weight (%IBW), percent standard triceps skinfold thickness (%TSF) and percent arm muscle circumference (%std MUAMC) to be 20.5 ± 2.6 kg/m2, 90±12, 75 ±30 and 100±13, respectively. However, 37%, 56% and 70% of these patients had BMI, BW and MUAC less than the minimal cut-off levels. These data reflect the importance of energy depletion in these patients. The average serum albumin was 39 ± 6 g/L while 52% suffered from low albumin levels. TC, LDL-C, HDL-C and TG were 4.34 ± 1.34, 2.9 ±1.06, 0.89 ± 0.33 and 1.10 ± 0.67 mmol/L while 15% had high TC, LDL-C and 58% had low HDL-C levels. Only one patient was suffered from hypertriglyceridemia. Whereas inadequate thiamine, riboflavin, ascorbic acid and α-tocopherol status were present in 4-30%, toxic levels of serum retinol were seen in almost all patients. Low serum zinc and copper levels were also present. This study disclosed a wide spectrum of nutritional problems usually overlooked in CRF patients undergoing chronic hemodialysis. Whereas zinc supplementation may be needed, vitamin A suppIementation in these patients should be | S. KOMINDR J. THIRAWITAYAKOM S. TAECHANGAM O. PUCHAIWATANANON S. SONGCHISOMBOON AND S. DOMRONGKITCHAIPORN(Department of Medicine and Research Center, Faculty of Medicine,Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand) (Department of M | 1996 | Biomedical and Environmental Sciences1996，9，2： | 1 |
| 18 | Postoperative complications after primary a- dult optical penetrating keratoplasty:prevalence and impact on graft survival 显示文摘 | Wagoner MD Ba-Abbad R Al-Mohaimeed M Al-Swailem S Zim-merman MB King Khaled Eye Specialist Hospital Corneal Trans- plant Study Group | 2009 | Cornea2009，28，4： | 1 |
| 19 | The use of molecular genetics in improvement of agricultural populations显示文摘 | DEKKERS J C M HOSPITAL F | 2002 | Nat Rev Genet2002，3，： | 1 |
| 20 | A Regional Case Study 显示文摘 | Brida J G Garrido N Deidda M Exploring the Dynamics of the Effi- ciency in the Italian Hospitality Sector | 2012 | Expert Systems with Applications2012，39，10： | 1 |
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