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| 1 | Proteomics-based analysis of functional proteins from fermented of compound wheat embryo Chinese medicine显示文摘Compound probiotics were used to ferment compound wheat embryo Chinesemedicine(CWECM)at 37°C for a given period,and the fermentation end pointwas determined based on pHvalue.The full spectrumof the proteinswithin the fermentation supernatant was determined by ion mass spectrometry,and then the fermentation was analyzed by proteomics.The difference of functional protein ofCWECMwas analyzed before and after fermentation.Additionally,the changes in flavor of peptides were examined.Our results indicated that when the fermentationwas performed for 96 h,the pH value became stable over time,which it shifted froman initial 3.98±0.04 to a stable 2.78±0.04.Proteomicbased analysis of the fermentation supernatant indicated that the fermentation of the probiotics increased.The amount of functional proteome and protein within the supernatant increased from 60 proteomes and 136 proteins before fermentation to 70 proteomes and 149 proteins post fermentation.These proteins were primarily members of the Ubiquitin and Histone families.These families contain more specific peptides after fermentation,and the coverage was reduced.Analysis of the unique P0C512 protein after fermentation indicated that this protein is an unstable,3Deffect columnar protein.We also found that probiotic fermentation can reduce the proportion of bitter peptideswithin fermentation broth from 47.49%to 36.98%,and the proportion of sweet peptides increased after fermentation from 35.29%to 51.01%.Based on testing,we concluded that fermentation of CWECM by compound probiotics can reduce the pH of the CWECM solution,increase the number of functional proteomes and proteins,improve the taste of CWECM solutions,and improve the therapeutic efficacy of CWECM. | Jihong Huang Xing Lyu Aimei Liao Yi Zhao Yinchen Hou Wenjing Chen Canrui Yang | 2019 | Grain & Oil Science and Technology2019，2，3： | 2 |
| 2 | Multi-protective effects of wheat embryo globulin on D-gal-induced aging mice显示文摘Wheat embryo globulin(WEG)has been proven to possess multiple biological activities,including antioxidative properties,immunomodulatory,and so on.Aged mouse model were established by subcutaneous injection of D-galactose(D-gal),and the effects of WEG on learning,memory,and antioxidant capacity in aging mice were explored through behavioural tests and antioxidant enzyme activities determination.Compared with the Model group,WEG improved the percentage of the platform quadrant,increased the number of crossing platforms,and enhanced the identification indexs.WEG also increased total antioxidant capacity(T-AOC),superoxide dismutase(SOD),and glutathione peroxidase(GSH-Px)activities in the liver and brains of aging mice,and reduced malondialdehyde(MDA)content.Pathological observations indicated that WEG protected against damage to brain in D-gal-induced aging mice.These results effectively revealed that WEG not only improved the abilities of learning and memory,and the cognitive impairment,but also delayed the aging process of the D-gal-induced mice. | Aimei Liao Xing Lyu Jiarui Ma Yinchen Hou Ming Hui Na Liu Yi Zhao Yixiang Cui Jihong Huang | 2023 | Food Science and Human Wellness2023，12，4： | 0 |
| 3 | Pectin-chitosan complex: Preparation and application in colon-specific capsule显示文摘Pectin-chitosan complex was prepared as a candidate material for colon-specific capsule.First,the effects of beating on the properties of complex films were explored.Fourier transform infrared(FT-IR)spectroscopy was used to analyze the reaction mechanics,and an in vitro simulation experiment was performed to determine the degradation performance.Second,the capsule was prepared using pectin-chitosan complex as the main raw material;its targeting effect was evaluated and analyzed.Results indicated that the film became smooth and compact by beating.FT-IR data indicated that cross-linking occurred between the hydroxyl group of pectin and the amino group of chitosan to form a network structure.In vitro experiment showed that the pectin-chitosan complex film could not be degraded in the simulated gastric and intestinal fluids.However,pectin-chitosan complex could be degraded in the simulated colonic fluid,so it could be a potential candidate for colon-specific capsule.Because the complex has no ability for forming hard capsule,the carrageenan and starch were added to increase the flexibility,gelation,transparency and mechanical properties of the film.The preferred composition suitable for preparing the capsule was a mass ratio(2:1:1)of pectin-chitosan/carrageenan/starch mixture.The capsule showed excellent features in terms of shape,water content,and brittleness.The drug release rate reached 93.33%in colonic fluid for 1 h when bovine serum albumin was the model drug.Therefore,pectin-chitosan may be considered as a new material for colon-targeted capsule. | Wu Li Wang Hao Zhu Xiaohua Hou Yinchen Liu Wangwang Yang Gongming Jiang Aimin | 2015 | International Journal of Agricultural and Biological Engineering2015，8，4： | 0 |
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