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| 1 | Differential control of growth,apoptotic activity and gene expression in human colon cancer cells by extracts derived from medicinal herbs,Rhazya stricta and Zingiber officinale and their combination显示文摘AIM:To investigate the effects of extracts from Rhazya stricta(R.stricta) and Zingiber officinale(Z.officinale) on human colorectal cancer cells.METHODS:Human colorectal cancer cells(HCT116) were subjected to increasing doses of crude alkaloid extracts from R.stricta(CAERS) and crude flavonoid extracts from Z.officinale(CFEZO).Cells were then harvested after 24,48 or 72 h and cell viability was examined by trypan blue exclusion dye test; clonogenicity and soft agar colony-forming assays were also carried out.Nuclear stain(Hoechst 33342),acridine orange/ethidium bromide double staining,agarose gel electrophoresis and comet assays were performed to assess pro-apoptotic potentiality of the extracts.Quantitative reverse transcription-polymerase chain reaction(qRT-PCR),using gene-specific primers and Western blot analyses were performed to assess the impact of CAERS and CFEZO on the expression levels of key regulatory proteins in HCT116 cells.RESULTS:Treatment with a combination of CAERS and CFEZO synergistically suppressed the proliferation,colony formation and anchorage-independent growth of HCT116 cells.Calculated IC50,after 24,48 and 72 h,were 70,90 and 130 μg/mL for CAERS,65,85 and 120 μg/mL for CFEZO and 20,25 and 45 μg/mL for both agents,respectively.CAERS- and CFEZO-treated cells exhibited morphologic and biochemical features of apoptotic cell death.The induction of apoptosis was associated with the release of mitochondrial cytochrome c,an increase in the Bax/Bcl-2 ratio,activation of caspases 3 and 9 and cleavage of poly ADP-ribose polymerase.CAERS and CFEZO treatments downregulated expression levels of anti-apoptotic proteins including Bcl-2,Bcl-X,Mcl-1,survivin and XIAP,and upregulated expression levels of proapoptotic proteins such as Bad and Noxa.CAERS and CFEZO treatments elevated expression levels of the oncosuppressor proteins,p53,p21 and p27,and reduced levels of the oncoproteins,cyclin D1,cyclin/cyclin-dependent kinase-4 and c-Myc.CONCLUSION:These data suggest that a combination of CAERS and CFEZO is a promising treatment for the prevention of colon cancer. | Ayman I Elkady Rania Abd El Hamid Hussein Osama A Abu-Zinadah | 2014 | World Journal of Gastroenterology2014，20，41： | 12 |
| 2 | Real time endoscopic ultrasound elastography and strain ratio in the diagnosis of solid pancreatic lesions显示文摘AIM To evaluate the accuracy of the elastography score combined to the strain ratio in the diagnosis of solid pancreatic lesions(SPL). METHODS A total of 172 patients with SPL identified by endoscopic ultrasound were enrolled in the study to evaluate the efficacy of elastography and strain ratio in differentiating malignant from benign lesions. The semi quantitative score of elastography was represented by the strain ratio method. Two areas were selected, area(A) representing the region of interest and area(B) representing the normal area. Area(B) was then divided by area(A). Sensitivity, specificity, positive predictive value(PPV), negative predictive value(NPV), and accuracy were calculated by comparing diagnoses made by elastography, strain ratio and final diagnoses.RESULTS SPL were shown to be benign in 49 patients and malignant in 123 patients. Elastography alone had a sensitivity of 99%, a specificity of 63%, and an accuracy of 88%, a PPV of 87% and an NPV of 96%. The best cut-off level of strain ratio to obtain the maximal area under the curve was 7.8 with a sensitivity of 92%, specificity of 77%, PPV of 91%, NPV of 80% and an accuracy of 88%. Another estimated cut off strain ratio level of 3.8 had a higher sensitivity of 99% and NPV of 96%, but with less specificity, PPV and accuracy 53%, 84% and 86%, respectively. Adding both elastography to strain ratio resulted in a sensitivity of 98%, specificity of 77%, PPV of 91%, NPV of 95% and accuracy of 92% for the diagnosis of SPL. CONCLUSION Combining elastography to strain ratio increases the accuracy of the differentiation of benign from malignant SPL. | Hussein Okasha Shaimaa Elkholy Ramy El-Sayed Mohamed-Naguib Wifi Mohamed El-Nady Walid El-Nabawi Waleed A El-Dayem Mohamed I Radwan Ali Farag Yahya El-sherif Emad Al-Gemeie Ahmed Salman Mohamed El-Sherbiny Ahmed El-Mazny Reem E Mahdy | 2017 | World Journal of Gastroenterology2017，23，32： | 11 |
| 3 | Anti-cyclic citrullinated peptide (anti-CCP) antibody in juvenile idiopathic arthritis (JIA):correlations with discase activity and scverity of joint damage (a multicenter trial)显示文摘 | Omar A Abo-Elyoun I Hussein H | | 0，，： | 1 |
| 4 | Impact of carbapenem re- sistance on the outcome of patients' hospital-acquired bacteraemia caused by Klebsiella pneumoniae 显示文摘 | Hussein K Raz-Pasteur A Kassis I | 2013 | The Journal of Hospital Infec- tion2013，83，4： | 1 |
| 5 | Oil spill sorption using carbonized pith bagasse: Application of carbonized pith bagasse as loose fiber 显示文摘 | HUSSEIN M AMER A A SAWSAN I I | 2009 | Global NEST Journal2009，11，4： | 1 |
| 6 | A review of charging algorithms for nickel and lithium battery chargers显示文摘 | HUSSEIN A A-H BATARSEH I | 2011 | IEEE Transactions on Vehicular Technology2011，60，3： | 1 |
| 7 | Tannins from the leaves of Punica granatum显示文摘 | Hussein S A M Barakat H H Merfort I | 1997 | Phyto- chemistry1997，45，4： | 1 |
| 8 | Acetylcholinesterase inhibitory effects of the bulb of Ammocharis coranica(Amaryllidaceae)and its active constituent lycorine显示文摘 | ELISHA I L ELORASHI E E HUSSEIN A A | 2013 | South African Journal of Botany2013，385，： | 1 |
| 9 | Photocatalytic treatment of 4-chlorophenol in aqueous ZnO suspensions: intermediates, influence of dosage and inorganic anions 显示文摘 | Gaya U I Abdullah A H Zainal Z Hussein M Z | 2009 | Journal of Hazardous Materials2009，168，1： | 1 |
| 10 | Rheological in- vestigation of the influence of short chain branching and Mw of LDPE on the melt miscibility of LDPE/PP blends 显示文摘 | Suleiman M A Hussein I A Williams M C | 2011 | Open Macromolecules Journal2011，5，1： | 1 |
| 11 | Viscoelastic properties of a high temperature cross-linked water shut-off polymeric gel显示文摘 | Al-Muntasheri G A Hussein I A Nasr-El-Din H A | 2007 | Journal of Petroleum Scinece and Engineering2007，55，3： | 1 |
| 12 | Characterization of Ti02-chitosaniglass photocatalyst for the removal of a monoazo dye via photodegradation-adsorption process显示文摘 | Zainal Z Hui L K Hussein M Z Abdullah A H Hamadneh I R | 2009 | Jour?nal of Hazardous Materials2009，164，1： | 1 |
| 13 | A rheological investigation of a high temperature organic gel used for water shut-off treatments显示文摘 | Al-Muntasheri G A Nasr-E1-Din H A Hussein I A | 2007 | Journal of Petroleum Scinece and Engineering2007，59，2： | 1 |
| 14 | Tannins from the leaf of Punica granatum显示文摘 | Hussein S A M Barakt H H Merfort I | 1997 | Phytochemistry1997，45，4： | 1 |
| 15 | Photo- catalytic treatment of 4--chlorophenol in aqueous ZnO sus- pensions: Intermediates, influence of dosage and inorganic anions 显示文摘 | Gaya U I Abdullah A H Zainal Z Hussein M Z | 2009 | J Hazard Mater2009，168，： | 1 |
| 16 | Evaluation of serum squamous cell carcinoma antigen as a novel biomarker for diagnosis of hepatocellular carcinoma in Egyptian patients显示文摘 | M Hussein A Ibrahim H Abdella I Montasser M Hassan | 2008 | Indian Journal of Cancer2008，，4： | 1 |
| 17 | Cytol- ogy and clinical spectrum of sexually transmitted infections in Lebanese women as revealed by Pap smear:a cross sectional study from 2002- 2006显示文摘 | Karam W G Rady A Abdallah Hajj Hussein I | 2011 | J Biol Regul Homeost Agents2011，25，3： | 1 |
| 18 | Oil Spill Sorption Using Carbonized Pith Bagasse: Trial for Practical Application 显示文摘 | Hussein M Amer A A Sawsan I I | 2008 | International Journal of Environmental Science and Technology2008，5，2： | 1 |
| 19 | Impact of particle emissions of new laser printers on modeled of- fice room 显示文摘 | Koivisto A J Hussein T Niemel~i R | 2010 | Atmospheric Environment2010，44，17： | 1 |
| 20 | Dispersive elastodynamics of 1 d banded materials and structures:Design 显示文摘 | Hussein Mahmoud I Hulbert Gregory M Scott Richard A | 2007 | Journal of Sound and Vibration2007，307，35： | 1 |
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