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您的检索式：作者名="Ian T. Christensen"
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| 1 | miR-211 regulates the antioxidant function of lens epithelial cells affected by age-related cataracts显示文摘AIM: To investigate the effects and mechanism of miR-211 in mediating the antioxidant function of lens epithelial cells affected by age-related cataracts.METHODS: Real-time quantitative polymerase chain reaction(RT-q PCR) was used to detect miR-211 expression in the anterior lens capsules of healthy people, the anterior lens capsules of patients with age-related cataracts, and human epithelial cell line(SRA01/04) cells exposed to oxidative stress. A 2', 7'-dichloro-fluorescein diacetate(DCFH-DA) probe was used to measure the levels of endogenous reactive oxygen species(ROS) in human lens epithelial cells(h LECs) exposed to 400 μmol/L H_2O_2 for 1 h. SRA01/04 cells were transfected with either miR-211 mimics, mimic controls, miR-211 inhibitors or inhibitor controls. After 72 h, these cells were exposed to 400 μmol/L H_2O_2 for 1 h, then p53 and Bax m RNA expression were measured using RT-q PCR. p53 and Bax protein expression were also measured by Western blotting analysis. Finally, cell viability was assessed using an MTS assay.RESULTS: Compared to the control group, expression of miR-211 in the anterior lens capsules of age-related cataract patients and in SRA01/04 cells exposed to oxidative stress was significantly increased(P<0.001). Levels of endogenous ROS were significantly elevated in h LECs exposed to oxidative stress(P<0.001). Compared to the mimic control group, the h LECs in the miR-211 mimic group expressed significantly higher levels of p53 and Bax m RNA and protein while cell viability was significantly reduced(P<0.001). Conversely, p53 and Bax m RNA and protein expression were significantly reduced in the miR-211 inhibitor group as compared to the control group, while the cells in this group had much higher levels of cell viability(P<0.001).CONCLUSION: miR-211 is upregulated in the anterior lens capsules of age-related cataract patients. miR-211 decreased the antioxidative stress capacity of lens epithelial cells by upregulating p53 and Bax, while inhibiting cell proliferation and repair. This finding suggests that miR-211 may play a key role in the development of age-related cataracts. | Bo Lu Ian T. Christensen Li-Wei Ma Tao Yu Ling-Feng Jiang Chun-Xia Wang Li Feng Jin-Song Zhang Qi-Chang Yan Xin-Ling Wang | 2018 | International Journal of Ophthalmology(English edition)2018，11，3： | 4 |
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