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| 1 | Cell-block procedure in endoscopic ultrasound-guided-fine-needle-aspiration of gastrointestinal solid neoplastic lesions显示文摘In the present review we have analyzed the clinical applications of endoscopic ultrasound-guided-fineneedle-aspiration(EUS-FNA) and the methodological aspects obtained by cell-block procedure(CBP) in the diagnostic approach to the gastrointestinal neoplastic pathology. CBP showed numerous advantages in comparison to the cytologic routine smears; in particular, better preservation of cell architecture, achievement of routine haematoxylin-eosin staining equivalent to histological slides and possibility to perform immunohistochemistry or molecular analyses represented the most evident reasons to choose this method. Moreover, by this approach, the differential diagnosis of solid gastrointestinal neoplasias may be more easily achieved and the background of contaminant nonneoplastic gastrointestinal avoided. Finally, biological samples collected by EUS-FNA CBP-assisted should be investigated in order to identify and quantify further potential molecular markers. | Antonio Ieni Valeria Barresi Paolo Todaro Rosario Alberto Caruso Giovanni Tuccari | 2015 | World Journal of Gastrointestinal Endoscopy2015，7，11： | 6 |
| 2 | Phagocytosis(cannibalism) of apoptotic neutrophils by tumor cells in gastric micropapillary carcinomas显示文摘AIM:To identify those with a micropapillary pattern,ascertain relative frequency and document clinicopathological characteristics by reviewing gastric carcinomas.METHODS:One hundred and fifty-one patients diagnosed with gastric cancer who underwent gastrectomy were retrospectively studied and the presence of a regional invasive micropapillary component was evaluated by light microscopy.All available hematoxylin-eosin(HE)-stained slides were histologically reviewed and 5 tumors were selected as putative micropapillary carcinoma when cancer cell clusters without a vascular core within empty lymphatic-like space comprised at least5%of the tumor.Tumor tissues from these 5 invasive gastric carcinomas were immunostained using an antimucin 1(MUC1)antibody(clone MA695)to detect the characteristic inside-out pattern and with D2-40antibody to determine the presence of intratumoral lymph vessels.Detection of intraepithelial neutrophil apoptosis was evaluated in consecutive histological tissue sections by three independent methods,namely light microscopy with HE staining,the conventional terminal deoxynucleotidyl transferase-mediated d UTP-biotin nick end-labeling(TUNEL)method and immunohistochemistry for activated caspase-3(clone C92-605).RESULTS:Among 151 gastric cancers resected for cure,5(3.3%)were adenocarcinomas with a micropapillary component.Four of the patients died of disease from 6 to 23 mo and one patient was alive with metastases at 9 mo.All patients had advanced-stage cancer(≥p T2)and lymph node metastasis.Positive MUC1 immunostaining on the stroma-facing surface(inside-out pattern)of the carcinomatous cluster cells,together with negative immunostaining for D2-40 in the cells limiting lymphatic-like spaces,confirmed the true micropapillary pattern in these gastric neoplasms.In all five cases,several micropapillae were infiltrated by neutrophils.HE staining,TUNEL assay and immunostaining for caspase-3 demonstrated apoptoticneutrophils within cytoplasmic vacuoles of tumor cells.These data suggest phagocytosis(cannibalism)of apoptotic neutrophils by micropapillary tumor cells.Tumor cell cannibalism is usually found in aggressive tumors with anaplastic morphology.Our data extend these observations to gastric micropapillary carcinoma:a tumor histotype analogously characterized by aggressive behavior and poor prognosis.The results are of interest because they raise the intriguing possibility that neutrophil cannibalism by tumor cells may be one of the mechanisms favoring tumor growth in gastric micropapillary carcinomas.CONCLUSION:This is the first study showing phagocytosis(cannibalism)of apoptotic neutrophils by tumor cells in gastric micropapillary carcinomas. | Valeria Barresi Giovanni Branca Antonio Ieni Luciana Rigoli Giovanni Tuccari Rosario Alberto Caruso | 2015 | World Journal of Gastroenterology2015，21，18： | 4 |
| 3 | Endoscopic ultrasound-guided fine-needle aspiration cytology in pancreaticobiliary carcinomas:diagnostic efficacy of cell-block immunocyto-chemistry显示文摘BACKGROUND:Endoscopic ultrasound-guided fine-needle aspiration cytology was demonstrated to be a useful tool for the diagnosis and staging of pancreaticobiliary neoplastic lesions.Nonetheless,the diagnostic value of this procedure may be limited by low cellularity of the specimen,contamination of intestinal cells and unfeasibility of ancillary immunocytochemical procedures.The present study was to evaluate its usefulness in the diagnosis of neoplastic lesions.METHODS:A series of 46 pancreaticobiliary carcinomas with available cell block preparations was submitted to immunocytochemistry against cytokeratins,carcinoembryonic antigen,E-cadherin,CD10 and p53.The sensitivity,specificity,positive and negative predictive values of the cytological smear in the discrimination of malignant lesions were calculated and compared with those of cell block preparation with the immunocytochemical stains against p53 and CD10.RESULTS:According to our findings,the use of cell block preparations together with immunostains against p53 and CD10allowed to discriminate malignant versus benign specimens with higher sensitivity than the only cytological examination.In detail,CD10 immunostaining was of significant help for the discrimination between cytological contaminants,such as benign gastrointestinal cells,and the neoplastic elements of pancreaticobiliary well differentiated adenocarcinomas.Also,intense nuclear immunoreactivity for p53 was encountered in about 2/3 of the cases and identified pancreatic malignancy with high sensitivity.CONCLUSIONS:We suggest that immunocytochemistry against both CD10 and p53 could be applied case by case,mainly to differentiate gastrointestinal and pancreatic benign cellular contaminants showing hyperplasia or reactive changes from differentiated pancreaticobiliary adenocarcinomas. | Antonio Ieni Paolo Todaro Stefano Francesco Crinò Valeria Barresi Giovanni Tuccari | 2015 | Hepatobiliary & Pancreatic Diseases International2015，14，3： | 4 |
| 4 | Discordance rate of HER2 status in primary breast carcinomas versus synchronous axillary lymph node metastases: a multieenter retrospective investigation 显示文摘 | Ieni A Ban'esi V Caltabiano R | 2014 | Onco Targets Ther2014，7，： | 1 |
| 5 | HER-2 Status in Premalignant,Early,and Advanced Neoplastic Lesions of the Stomach显示文摘 | Ieni A Barresi V Rigoli L | 2015 | Disease Markers2015，2015，23： | 1 |
| 6 | Antiamnesic and neuroprotective effects of donepezil against learning impairments induced in mice by exposure to carbon monoxide gas显示文摘 | MEUNIER J IENI J MAURICE T | 2006 | J Pharmacol Exp Ther2006，317，3： | 1 |
| 7 | Lactoferrin immuno-ex- pression in human normal and neoplastic bone tissue 显示文摘 | Ieni A Barresi V Grosso M et d | 2009 | Bone Miner Metab2009，27，3： | 1 |
| 8 | Limits of fine-needle aspiration cytology in diagnosing pilomatfixoma: a series of 25 cases with clinico- pathologic correlations 显示文摘 | Ieni A Todaro P Bonanno AM | 2012 | Indian J Dermatol2012，57，2： | 1 |
| 9 | A placebo-controlled evaluation of single, escalating doses of CL 284,846 ,a non-benzodiazepine hypnotic显示文摘 | Beer B Ieni JR Wu W H | 1994 | J Pharmacol1994，34，： | 1 |
| 10 | A placebo-controlled evaluation of single,escalating doses of CL 284,846,a non-benzodiazepine hypnotic显示文摘 | Beer B Ieni JR Wu WH | 1994 | J Clin Pharmacol1994，34，4： | 1 |
| 11 | Parathyroid carcinoma presenting as normocalcemic hyperparathyroidism显示文摘 | Campennì A Ruggeri RM Sindoni A Giovinazzo S Calbo E Ieni A Calbo L Tuccari G Baldari S Benvenga S | | 0，，： | 1 |
| 12 | Neutrophil gelatinase-associated lipocalin immunoexpression in renal tumors: correlation with histotype and histological grade显示文摘 | BARRESI V IENI A BOLIGNANO D | 2010 | Oncol Rep2010，24，2： | 1 |
| 13 | A placebo-controlled evalu ation of single, escalating doses of CL-284, 846, a non benzodiazepine hyponotic显示文摘 | Beer B Ieni JR Wu WH | 1994 | J Clin Pharmacol1994，34，： | 1 |
| 14 | HER2 status in unusual histo-logical variants of gastric adenocarcinomas 显示文摘 | Giuffrfe G Ieni A Barresi V | 2012 | J Clin Pathol2012，65，3： | 1 |
| 15 | A placebo-controlled evalution of single ,escalating doses of CL 284,846, a non-benzodiazepine hypnotic显示文摘 | Beer B Ieni JR Wu WH | 1994 | J Clin Pharmacol1994，34，： | 1 |
| 16 | A placebo-controlled evaluation of single,escalating doses of CL284,846,a nonbenzodiazepine hypnotic显示文摘 | BEER B IENI J R WU W H | 1994 | J Clin Pharmacol1994，34，： | 1 |
| 17 | A placebo-controlled evaluation of single,escalating doses of CL 284,846,a non-benzodiazepine hypnotic 显示文摘 | Beer B Ieni JR Wu WH | 1994 | J Clin Pharmacol1994，34，4： | 1 |
| 18 | Uterine large cell neuroendocrine carcinoma with unusual colonic metastasis显示文摘A 78-year-old female patient arrived at our practice complaining of progressive abdominal increase and presenting a clinical picture of intestinal obstruction.At physical examination,the abdomen appeared distended,moderately painful with the presence of a mass of hard consistency.Abdominal computed tomography scan showed a large hypodense pelvic mass that indicated a compression and lateral deviation of the uterus and bladder.Microscopically,the mass showed a uniform solid pattern,composed of medium and large-sized cells with hyperchromatic and pleomorphic nuclei demonstrating high mitotic activity and diffuse immunoreactivity for estrogen receptors and synaptophysin.A diagnosis of uterine poorly differentiated large cell neuroendocrine carcinoma,arising in the endometrium with an unusual colonic metastatic localization,was made. | Antonio Ieni Giuseppe Angelico Rosalba De Sarro Francesco Fleres Antonio Macrì Giovanni Tuccari | 2017 | Journal of Cancer Metastasis and Treatment2017，3，1： | 1 |
| 19 | HER2 status in unusual histological variants of gastric adenocarcinomas显示文摘 | Giuffrè G Ieni A Barresi V | | 0，，03： | 1 |
| 20 | Intraparietal esophageal leiomyomas diagnosed by endoscopic ultrasound?guidedfine?needle aspiration cytology: Cytological and immunocytochemical features intwo cases显示文摘 | P. Todaro S. Crinò A. Ieni S. Pallio P. Consolo G. Tuccari | 2014 | Oncology Letters2014，，1： | 1 |
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