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| 1 | DNA-PKcs subunits in radiosensitization by hyperthermia on hepatocellular carcinoma hepG_2 cell line显示文摘AIM: To investigate the role of DNA-PKcs subunits inradiosensitization by hyperthermia on hepatocellularcarcinoma HepG2 cell lines.METHODS: Hep G2 cells were exposed to hyperthermiaand irradiation. Hyperthermia was given at 45.5 ℃Cellsurvival was determined by an in vitro clonogenic assay forthe cells treated with or without hyperthermia at varioustime points. DNA DSB rejoining was measured usingasymmetric field inversion gel electrophoresis (AFIGE). TheDNA-PKcs activities were measured using DNA-PKcs enzymeassay system.RESULTS: Hyperthermia can significantly enhanceirradiation-killing cells. Thermal enhancement ratio ascalculated at 10 % survival was 2.02. The difference inradiosensitivity between two treatment modes manifestedas a difference in the α components and the almost sameβ components, which α value was considerably higher inthe cells of combined radiation and hyperthermia ascompared with irradiating cells (1.07 Gy-1 versus 0.44 Gy1). Survival fraction showed 1 logarithm increase after an8-hour interval between heat and irradiation, whereas DNA-PKcs activity did not show any recovery. The cells wereexposed to heat 5 minutes only, DNA-PKcs activity wasinhibited at the nadir, even though the exposure time waslengthened. Whereas the ability of DNA DSB rejoining wasinhibited with the increase of the length of hyperthermictime. The repair kinetics of DNA DSB rejoining aftertreatment with Wortmannin is different from thehyperthermic group due to the striking high slow rejoiningcomponent.CONCLUSION: Determination with the cell extracts andthe peptide phosphorylation assay, DNA-PKcs activity wasinactivated by heat treatment at 45.5 C, and could notrestore. Cell survival is not associated with the DNA-PKcsinactivity after heat. DNA-PKcs is not a unique factor affectingthe DNA DSB repair. This suggests that DNA-PKcs do notplay a crucial role in the enhancement of cellularradiosensitivity by hyperthermia. | Walter J.Curran George Iliakis | 2002 | World Journal of Gastroenterology2002，8，5： | 87 |
| 2 | 转化的大鼠胚胎成纤维细胞系差异表达基因的筛选研究显示文摘来源于转化的大鼠胚胎成纤维细胞系的两株细胞,A1-5细胞与B4细胞相比表现出非常强的抗辐射性并伴随不同寻常强的G2延迟效应;用PCR选择性抑制消减杂交方法对这两株细胞进行差减,希望找到对A1-5细胞表现出的不同寻常的表型起关键作用的某一个或某一些基因。结果得到了160个差减转化子,逐个进行序列测定,并进行Dotblot杂交,共得到35个差异表达基因片段(EST)。通过对美国国家生物技术信息中心(NCBI)的非冗余序列库(NT)、鼠EST库及人EST库的BLAST进行同源检索,发现其中21个代表了尚未登录的新基因,另外14个分别与已知基因高度同源。 | 胡宝成 王翔 George Iliakis 王亚 | 2002 | 生物技术通讯2002，13，2： | 8 |
| 3 | 转化的大鼠胚胎成纤维细胞系与p53^(val135)功能关系的研究显示文摘A1-5是一株典型的温度敏感的p53val135细胞系,许多实验室用它来研究p53的功能。我们首次报道了A1-5细胞表现出非常强的抗辐射性并伴随不同寻常强的G2延迟效应。B4是另一株温度敏感的p53val135细胞系,A1-5与B4细胞都是来源于同样的原代转化的大鼠胚胎成纤维细胞系,只是来自不同的转染实验。本文阐明了A1-5细胞非常强的抗辐射性及不同寻常强的G2停滞效应与p53val135的功能无关。A1-5细胞系具有的特殊表型是研究放射敏感性新性质的独特的模型。 | 胡宝成 Iliakis George 王亚 | 2002 | 生物技术通讯2002，13，1： | 7 |
| 4 | Bystander effects as manifestation of intercellular communication of DNA damage and of the cellular oxidative status显示文摘 | Klammer H Mladenov E Li F Iliakis G | 2015 | Cancer Lett2015，356，： | 1 |
| 5 | Backup pathways of nhej in cells of higher eu- karyotes : Cell cycle dependence 显示文摘 | Iliakis G | 2009 | Radiother Oncol2009，92，3： | 1 |
| 6 | Possible importance of PLD repair in the modulation of BrdUrd and IdUrd-mediated radiosensitization in plateau-phase C3H10T1/2 mouse embryo cells 显示文摘 | Iliakis G Wright E Ngo FQ | 1987 | Int J Radiat Biol Relat Stud Phys Chem Med1987，51，3： | 1 |
| 7 | Evidence for an S-phase checkpoint regulating DNA replication after heat shock 显示文摘 | Iliakis G Krieg T Guan J | 2004 | Int J Hyperthermia2004，20，2： | 1 |
| 8 | Down-regulation of DNA replication in extracts of camptothecin-treated cells:activation of an S-phase checkpoint?显示文摘 | Wang Y Perrault AR Iliakis G | 2007 | Cancer Res2007，57，9： | 1 |
| 9 | Application of non-hypoxic cell sensitizers in radiobiology and radiotherapy:rationale and future prospects显示文摘 | Iliakis G Kurtzman S | 1989 | Int J Radiat Oncol Biol Phys1989，16，5： | 1 |
| 10 | Rationalization of the activity of medical ozone on intervertebral diac:a histological and biochemical study显示文摘 | Iliakis E Valadakis V Vynios DH | 2001 | Riv Neuroradiol2001，14，1： | 1 |
| 11 | Induction and repair of DNA double strand breaks : The increasing spectrum of non-homologans end joining pathways 显示文摘 | Mladenov E Iliakis G | 2011 | Mutat Res2011，711，12： | 1 |
| 12 | Effects of hyperthermia on the repair of radiation-induced DNA single-and double-strand breaks in DNA double-strand break repair-deficient and repair-proficient cell lines显示文摘 | Iliakis G Seaner R Okayasu R | 1990 | Int J Hyperthermia1990，6，4： | 1 |
| 13 | Rationalization of the activity of medical ozone on intervertebral diac:a histological and biochemical study显示文摘 | Iliakis E Valadakis V Vynios DH | 2001 | Riv Neuroradiol2001，14，1： | 1 |
| 14 | Evidence for an S-phase check- point regulating DNA replication after heat shock显示文摘 | Iliakis G Krieg T Guan J | 2004 | Int J Hy- per-thermia2004，20，2： | 1 |
| 15 | Evidence for an S-phase checkpoint regulating DNA replication after heat shock: a re- view显示文摘 | Iliakis G Krieg T Guan J | 2004 | International Journal of Hyperthermia2004，20，2： | 1 |
| 16 | GammaH2AX in recognition and signaling of DNA doublestrand breaks in the context of chromatin显示文摘 | Kinner A Wu W Staudt C Iliakis G | 2008 | Nucleic Acids Res2008，36，17： | 1 |
| 17 | Clinical outcomes in descemet stripping automated endothelial keratoplasty with internationally shipped precut donor corneas 显示文摘 | NAKAGAWA H INATOMI T HIEDA O SOTOZONO C YOKOI N ILIAKIS B | 2014 | Am J Ophthalmol2014，157，1： | 1 |
| 18 | Photothrombosis of retinal and choroidal vessels in rabbit eyes using chloroaluminum sulfonated phthalocyanine and a diode laser显示文摘 | Iliaki OE Naoumidi II Tsilimbaris MK | 1996 | Lasers Surg Med1996，19，3： | 1 |
| 19 | Complex H2AX phosphorylation patterns by multiple kinases including ATM and DNA-PK in human cells exposed to ionizing radiation and treated with kinase inhi-bitors显示文摘 | Wang H Wang M Wang H Bocker W Iliakis G | 2005 | J Cell Physiol2005，202，2： | 1 |
| 20 | Rationalization of the activity of medical ozone on intervertebral diac:a histological and biochemical study显示文摘 | Iliakis E Valadakis V Vynios DH | 2001 | Riv Neuroradiol2001，14，1： | 1 |
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