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您的检索式：作者名="Iliakis George"
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| 1 | DNA-PKcs subunits in radiosensitization by hyperthermia on hepatocellular carcinoma hepG_2 cell line显示文摘AIM: To investigate the role of DNA-PKcs subunits inradiosensitization by hyperthermia on hepatocellularcarcinoma HepG2 cell lines.METHODS: Hep G2 cells were exposed to hyperthermiaand irradiation. Hyperthermia was given at 45.5 ℃Cellsurvival was determined by an in vitro clonogenic assay forthe cells treated with or without hyperthermia at varioustime points. DNA DSB rejoining was measured usingasymmetric field inversion gel electrophoresis (AFIGE). TheDNA-PKcs activities were measured using DNA-PKcs enzymeassay system.RESULTS: Hyperthermia can significantly enhanceirradiation-killing cells. Thermal enhancement ratio ascalculated at 10 % survival was 2.02. The difference inradiosensitivity between two treatment modes manifestedas a difference in the α components and the almost sameβ components, which α value was considerably higher inthe cells of combined radiation and hyperthermia ascompared with irradiating cells (1.07 Gy-1 versus 0.44 Gy1). Survival fraction showed 1 logarithm increase after an8-hour interval between heat and irradiation, whereas DNA-PKcs activity did not show any recovery. The cells wereexposed to heat 5 minutes only, DNA-PKcs activity wasinhibited at the nadir, even though the exposure time waslengthened. Whereas the ability of DNA DSB rejoining wasinhibited with the increase of the length of hyperthermictime. The repair kinetics of DNA DSB rejoining aftertreatment with Wortmannin is different from thehyperthermic group due to the striking high slow rejoiningcomponent.CONCLUSION: Determination with the cell extracts andthe peptide phosphorylation assay, DNA-PKcs activity wasinactivated by heat treatment at 45.5 C, and could notrestore. Cell survival is not associated with the DNA-PKcsinactivity after heat. DNA-PKcs is not a unique factor affectingthe DNA DSB repair. This suggests that DNA-PKcs do notplay a crucial role in the enhancement of cellularradiosensitivity by hyperthermia. | Walter J.Curran George Iliakis | 2002 | World Journal of Gastroenterology2002，8，5： | 87 |
| 2 | 转化的大鼠胚胎成纤维细胞系差异表达基因的筛选研究显示文摘来源于转化的大鼠胚胎成纤维细胞系的两株细胞,A1-5细胞与B4细胞相比表现出非常强的抗辐射性并伴随不同寻常强的G2延迟效应;用PCR选择性抑制消减杂交方法对这两株细胞进行差减,希望找到对A1-5细胞表现出的不同寻常的表型起关键作用的某一个或某一些基因。结果得到了160个差减转化子,逐个进行序列测定,并进行Dotblot杂交,共得到35个差异表达基因片段(EST)。通过对美国国家生物技术信息中心(NCBI)的非冗余序列库(NT)、鼠EST库及人EST库的BLAST进行同源检索,发现其中21个代表了尚未登录的新基因,另外14个分别与已知基因高度同源。 | 胡宝成 王翔 George Iliakis 王亚 | 2002 | 生物技术通讯2002，13，2： | 8 |
| 3 | 转化的大鼠胚胎成纤维细胞系与p53^(val135)功能关系的研究显示文摘A1-5是一株典型的温度敏感的p53val135细胞系,许多实验室用它来研究p53的功能。我们首次报道了A1-5细胞表现出非常强的抗辐射性并伴随不同寻常强的G2延迟效应。B4是另一株温度敏感的p53val135细胞系,A1-5与B4细胞都是来源于同样的原代转化的大鼠胚胎成纤维细胞系,只是来自不同的转染实验。本文阐明了A1-5细胞非常强的抗辐射性及不同寻常强的G2停滞效应与p53val135的功能无关。A1-5细胞系具有的特殊表型是研究放射敏感性新性质的独特的模型。 | 胡宝成 Iliakis George 王亚 | 2002 | 生物技术通讯2002，13，1： | 7 |
| 4 | G_1和G_2期细胞对X射线引起双链DNA断裂修复的影响显示文摘目的:探讨X射线对G_1和G_2期细胞双链DNA断裂损伤修复的动力学影响。方法:采用3%多聚甲醛或70%乙醇固定受X射线放射细胞的M059J、M059K和Hela,用流式细胞仪分选G_1和G_2期Hela细胞,以脉冲场电泳分析双链DNA断裂修复动力学。结果:乙醇固定不影响X射线放射引起的双链DNA断裂修复动力学,可用于固定Hela细胞G_1和G_2期的分选,且G_2期细胞双链DNA断裂修复速率显著慢于G_1期细胞。结论:G_1和G_2期细胞中的双链DNA断裂可能采用不同的修复方式。G_1期细胞主要采用快速的末端连接修复方式,而G_2期细胞主要采用相对较慢的同源性重组修复方式。 | 吴伟忠 George Iliakis 刘康达 | 2007 | 中国临床医学2007，14，6： | 0 |
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