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| 1 | Second-line Therapy With Levofloxacin After Failure of Treatment to Eradicate Helicobacter pylori Infection: Time Trends in a Spanish Multicenter Study of 1000 Patients显示文摘 | Javier P. Gisbert ángeles Pérez-Aisa Fernando Bermejo Manuel Castro-Fernández Pedro Almela Jesús Barrio ángel Cosme Inés Modolell Felipe Bory Miguel Fernández-Bermejo Luis Rodrigo Jesús Ortu?o Pilar Sánchez-Pobre Sam Khorrami Alejandro Franco Albert Tomas | 2013 | Journal of Clinical Gastroenterology2013，，2： | 5 |
| 2 | Preclinical evaluation of azathioprine plus buthionine sulfoximine in the treatment of human hepatocarcinoma and colon carcinoma显示文摘AIM: To evaluate the efficacy and the safety of azathioprine (AZA) and buthionine sulfoximine (BSO) bylocalized application into HepG2 tumor in vivo.METHODS: Different hepatoma and colon carcinoma cell lines (HepG2, HuH7, Chang liver, LoVo, RKO, SW-48, SW-480) were grown in minimal essencial medium supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic solution and maintained in a humidified 37 ℃ incubator with 5% CO2. These cells were pretreated with BSO for 24 h and then with AZA for different times. We examined the effects of this combination on some proteins and on cellular death. We also studied the eff icacy and the safety of AZA (6 mg/kg per day) and BSO (90 mg/kg per day) in HepG2 tumor growth in vivo using athymic mice. We measured safety by serological markers such as aminotransferases and creatine kinase.RESULTS: The in vitro studies revealed a new mechanism of action for the AZA plus BSO combination in the cancer cells compared with other thiopurines (6-mercaptopurine, 6-methylmercaptopurine, 6-thioguanine and 6-methylthioguanine) in combination with BSO. The cytotoxic effect of AZA plus BSO in HepG2 cells resulted from necroptosis induction in a mitochondrial-dependent manner. From kinetic studies we suggest that glutathione (GSH) depletion stimulates c-Jun amino-terminal kinase and Bax translocation in HepG2 cells with subsequent deregulation of mitochondria (cytochrome c release, loss of membrane potential), and proteolysis activation leading to loss of membrane integrity, release of lactate dehydrogenase and DNA degradation. Some of this biochemical and cellular changes could be reversed by N-acetylcysteine (a GSH replenisher). In vivo studies showed that HepG2 tumor growth was inhibited when AZA was combined with BSO.CONCLUSION: Our studies suggest that a combination of AZA plus BSO could be useful for localizedtreatment of hepatocellular carcinoma as in the currently used transarterial chemoembolization method. | Borja Hernández-Breijo Jorge Monserrat Sara Ramírez-Rubio Eva P Cuevas Diana Vara Inés Díaz-Laviada M Dolores Fernández-Moreno Irene D Román Javier P Gisbert Luis G Guijarro | 2011 | World Journal of Gastroenterology2011，17，34： | 2 |
| 3 | Emulsifying propertyof whey peptide fractions as a function of pH and IonicStrength显示文摘 | Turgeon S L Cauthier SF Paqu in P | 1992 | Food Sci1992，57，60： | 1 |
| 4 | Cross-national variation in the gender gap in depression 显示文摘 | Van de Velde S Bracke P Levecque Gender differences in de- pression in 23 European countries | 2010 | Soc Sci Med2010，71，2： | 1 |
| 5 | Nonexpanded Primary Lung and Bone Marrow-derived Mesenchymal Cells Promote the Engraftment of Umbilical Cord Blood-derived CD34^+ Cells in NOD/SCID Mice显示文摘 | In't Ankera P S Noortb W A Kruisselbrinkb A B | 2003 | Experimental Hematology2003，31，： | 1 |
| 6 | Hashimoto's encephalopathy:postmortem findings after fatal status epilepticus显示文摘 | Duffey P Yee S Reid IN | 2003 | Neurology2003，61，8： | 1 |
| 7 | Integrating XML with relational database using middleware approach显示文摘 | Computer Science and Information Lecture Notes in Computer Science Lum L C Lee S P | 2005 | Malaysian Journal of Computer Science2005，18，2： | 1 |
| 8 | 000-mobocular-weighl membrane protein modulates virus release显示文摘 | Liljestrom P Lusa S Huylebroeck D In vitro mutagenesis of full-length cDNA clone of Semliki Foresl virus: the 6 | 1991 | J Virol1991，65，： | 1 |
| 9 | Application of gas-diffusion microextraction to the analysis of free and bound acetaldehyde in wines by HPLC-UV and characterization of the extracted compounds by MS/MS detection显示文摘 | Manuel P Cruz Inês M Valente Luís M | 2012 | Analytical and Bioanalytical Chemistry2012，，403： | 1 |
| 10 | Diamond 显示文摘 | P Keblinski S R Philiipot D Wolf and H Gleiter on the Nature of Grain Boundaries in Nanocrystalline | 1999 | Nanostructured Materials1999，，12： | 1 |
| 11 | Retardation of polymer oxidation by natural antioxidant gossypol:Part 1-Kinetics of oxygen consumption显示文摘 | Mar'in A P Shlyapnikov Y A Mametov E S | 1992 | Polymer Degradation and Stability1992，35，2： | 1 |
| 12 | Mesenchymal stem cells promote engraftment of human umbilical cord blood-derived CD34^+ cells in NOD/SCID mice 显示文摘 | Noort W A Kruisselbrink A B In't Anker P S | 2002 | Exp Hematol2002，30，8： | 1 |
| 13 | Alkyldibenzofuranes in terrestrial rocks:Influence of organic facies and maturation显示文摘 | Radke M Vriend S P Ramanampisoa In R | 2000 | Geochimica et Cosmochimica Acta2000，64，2： | 1 |
| 14 | Mesenchymal stem ceils promote engraftment of human umbilical cord blood-derived CD34 (+) cells in NOD/SCID mice显示文摘 | Noort W A Kruisselbrink A B In't Anker P S | 2002 | Exp Hematol2002，30，8： | 1 |
| 15 | Hashimoto's encephalopathy postmortem findings after fatal status epilepticus 显示文摘 | Duffey P Yee S Reid IN | 2003 | Neurology2003，61，： | 1 |
| 16 | Diffusion-Limited Aggregation显示文摘 | MEAK IN P TOLMAN S | 1989 | Proc R Soc Lond A1989，423，： | 1 |
| 17 | japon- icum infections: a proof-of-concept study显示文摘 | van Dam GJ Odermatt P Acosta L Evaluation of banked urine samples for the detection of circulating anodic and cathodic antigens in Schistosoma mekongi and S | 2015 | Acta Trop2015，，： | 1 |
| 18 | 75Wt Pct Ti and W-Ni-Fe during high strain rate deformation显示文摘 | Zurek A K Follansbee P S A comparison of shear localization susceptibility in U-0 | 1995 | Met Trans A1995，26，： | 1 |
| 19 | ese black and white teenages显示文摘 | Owens S Cutin B Barrbeau P Visceral adipose tissue and markers of the insulin resistance syndrome in ob | 2000 | Obes Res2000，8，4： | 1 |
| 20 | Disease of the gallbladder and pancreas in pregnancy 显示文摘 | Ram in K D Ramsey P S | 2001 | Obstet Gynaecol Clin North Am2001，28，3： | 1 |
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