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| 1 | Puerarin prevents bone loss in ovariectomized mice and inhibits osteoclast formation in vitro显示文摘The present study aimed at investigating the effects of Puerarin(PR), a major isoflavonoid isolated from the Chinese medicinal herb Puerariae radix, on bone metabolism and the underlying mechanism of action. The in vivo assay, female mice were ovariectomized(OVX), and the OVX mice were fed with a diet containing low, middle, and high doses of PR(2, 4, and 8 mg·d^(-1), respectively) or 17β-estradiol(E_2, 0.03 μg·d^(-1)) for 4 weeks. In OVX mice, the uterine weight declined, and intake of PR at any dose did not affect uterine weight, compared with the control. The total femoral bone mineral density(BMD) was significantly reduced by OVX, which was reversed by intake of the diet with PR at any dose, especially at the low dose. In the in vitro assay, RAW264.7 cells were used for studying the direct effect of PR on the formation of osteoclasts. PR reduced the formation of tartrate resistant acid phosphatase(TRAP)-positive multi-nucleated cells in the RAW 264.7 cells induced by receptor activator for nuclear factor-κB Ligand(RANKL). MC3T3-E1 cells were used for studying the effects of PR on the expression of osteoprotegerin(OPG) and RANKL m RNA expression in osteoblasts. The expression of OPG m RNA and RANKL m RNA was detected by RT-PCR on Days of 5, 7, 10, and 12 after PR exposure. PR time-dependently enhanced the expression of OPG m RNA and reduced the expression of RANKL m RNA in MC3T3-E1 cells. In conclusion, our results suggest that PR can effectively prevent bone loss in OVX mice without any hyperplastic effect on the uterus, and the antiosteoporosis activity of PR may be related to its effects on the formation of osteoclasts and the expression of RANKL OPG in osteoblasts. | YUAN Si-Yuan SHENG Tong LIU Lian-Qi ZHANG Yun-Ling LIU Xue-Mei MA Tao ZHENG Hong YAN Yan ISHIMI Yoshiko WANG Xin-Xiang | 2016 | Chinese Journal of Natural Medicines2016，14，4： | 20 |
| 2 | Isoflavone Regulates Lipid Metabolism via Expression of Related Genes in OVX Rats Fed on a High-fat Diet显示文摘Objective To investigate the effects of isoflavone on body weight,fat mass,and gene expression in relation to lipid metabolism.Methods Thirty-six female SD rats were ovariectomized or sham-operated and fed on a high-fat diet.Two months later,abdominal incision was made,blood was collected to separate serum,and the liver and adipose tissue were immediately collected and weighed.Some portions of these tissues were frozen in liquid nitrogen and stored at -80℃.Results Ovariectomy(OVX) with a high-fat diet could induce obesity in rats,while treatment with isoflavone significantly inhibited the increase in body weight and fat mass in abdomen.Serum total cholesterol and leptin were significantly decreased in isoflavone group,compared with the OVX group.The mRNA expression of liver fatty acid synthase(FAS) in the OVX group was significantly higher than that in sham-operated group,while this difference was not observed in the isoflavone group.The mRNA expression of liver hormone-sensitive lipase(HSL) in the OVX rats tended to be lower than that in the sham-operated rats.Furthermore,a large amount of isoflavone maintained the mRNA expression at a sham level.Conclusion Isoflavone may prevent obesity induced by ovariectomy with a high-fat diet,in part by modulating gene expression related to lipid metabolism. | XIAO-LIN NA JUNKO EZAKI FUMIE SUGIYAMA HONG-BIN CUI AND YOSHIKO ISHIMI | 2008 | Biomedical and Environmental Sciences2008，21，5： | 2 |
| 3 | Phosphorylation of MCM4 at sites inaetivating DNA helicase activity of the MCM4-MCM6-MCM7 complex during Epstein-Barr virus productive Replication显示文摘 | Kudoh A Daikoku T Ishimi Y | 2006 | J Virol2006，80，10： | 1 |
| 4 | Difference in effective dosage of Genistein on bone and uterus in ovariectomized mice显示文摘 | Ishimi Y Arai N Wang X | 2002 | Biochem and Biophy Res Comm2002，，： | 1 |
| 5 | Selective effects of genistein, a soybean isoflavone on B-lymphopoiesis and bone loss caused by estradiol deficiency 显示文摘 | Ishimi Y Miyaura C Ohmura M | 1999 | Endocrinology1999，140，11： | 1 |
| 6 | Roles of MCM7 and MCM4 subunits in the DNA helicase activity of the mouse MCM4/6/7显示文摘 | You Z Ishimi Y Masai H | 2002 | J Biol Chem2002，277，42： | 1 |
| 7 | Difference in effective,dosage of genistein on bone and uterus in ovariectomized mice显示文摘 | Ishimi Y Arai N Wang X | 2000 | Biochem and Biophy Res Comm2000，274，： | 1 |
| 8 | Difference in effective dosage of genistein on bone and uterus in ovariectomized mice显示文摘 | Arai N Wang X | 2000 | Biochem Biophys Res Commun2000，274，3： | 1 |
| 9 | Inhibition of Mcm4,6,7 helicase activity by phosphorylation with cyclin A/Cdk2显示文摘 | Ishimi Y Komamura Kohno Y You Z | 2000 | J Biol Chem2000，275，16： | 1 |
| 10 | Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix显示文摘 | Ishimi Y Okayasu I Kato C | 2003 | Eur J Biochem2003，270，6： | 1 |
| 11 | Effects of extracted soy isoflavones alone on blood total and LDL cholesterol : Meta-analysis of randomized controlled trials 显示文摘 | Taku K Umegaki K Ishimi Y | 2008 | Ther Clin Risk Manag2008，4，5： | 1 |
| 12 | 显示文摘 | Ishimi Y | 2000 | Biochem Biophys Res Commun2000，274，3： | 1 |
| 13 | Enhanced expression of MCM proteins in cancer cells derived from uterine cervix 显示文摘 | Ishimi Y Okayasu I Kato C | 2003 | Eur J Biochem2003，270，6： | 1 |
| 14 | Roles of Mcm7 and Mcm4 subunits in the DNA heliease activity of the mouse Mere4/6/7 eomplex显示文摘 | You Z Ishimi Y Masai H | 2002 | Biol Chem2002，277，42： | 1 |
| 15 | Seasonal variations in office, home and 24 h ambulatory blood pressure in patients with essential hypertension显示文摘 | Minami J Kawano Y Ishimi*su T | 1996 | J Hypertens1996，14，12： | 1 |
| 16 | IL-6 is produce by osteoblasts and induces bone resorption显示文摘 | Ishimi YC Miyaura CH Jim T | 1990 | Immunol1990，145，6： | 1 |
| 17 | Difference in effective dosage of genistein on bone and uterus in ovariectomized mice显示文摘 | Ishimi Y Arai N Wang X | 2000 | Biochem Biophys Res Commun2000，274，3： | 1 |
| 18 | Difference in effective dosage of genistein on bone and uterus in ovariectomized mice 显示文摘 | Ishimi Y Arai N Wang X | 2000 | Biochem Biophys Res Commun2000，274，： | 1 |
| 19 | Biochemical function of mouse minichromosome maintenance protein 2 显示文摘 | Ishimi Y Komamura Y You Z | 1998 | J Biol Chem1998，273，： | 1 |
| 20 | IL-6 is produced by osteoblasts and induces bone resorption 显示文摘 | Ishimi Y Miyaura C Jin C H | 1990 | J Immunol1990，145，10： | 1 |
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