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| 1 | 2018年急性缺血性卒中患者早期管理指南美国心脏协会/美国卒中协会为医疗专业人员制定的指南显示文摘背景和目的本指南旨在在单个文件中为治疗成年急性动脉性缺血性卒中患者的临床医生提供最新全面的系列推荐意见。目标读者为院前急救人员、医生、综合医疗保健人员和医院管理人员。本指南将取代2013年版指南及其后续更新。方法写作组成员由美国心脏协会卒中委员会的科学声明监督委员任命,代表各领域的医学专家。严格遵循美国心脏协会的利益冲突原则。不允许写作组成员对存在企业利益关系的相关议题进行讨论或投票。所有推荐意见必须得到写作组成员的一致通过,除非企业利益关系妨碍了成员投票。由4名同行评议专家以及卒中委员会的科学声明监督委员会和领导委员会成员对指南草案进行发布前评审。本指南采用了美国心脏病学学会/美国心脏协会2015年推荐意见分类和证据级别标准以及新版美国心脏协会指南格式。结果本指南详细介绍了院前医疗、紧急和急诊评估、静脉和血管内治疗以及院内管理,包括在发病后最初2周内启用的二级预防措施。本指南支持院前和院内卒中医疗系统的一体化概念。结论本指南基于目前可获得的最佳证据。然而,许多情况资料有限,迫切需要对急性缺血性卒中的治疗进行持续研究。 | William J. Powers Alejandro A. Rabinstein Teri Ackerson Opeolu M. Adeoye Nicholas C. Bambakidis Kyra Becker José Biller Michael Brown Bart M. Demaerschalk Brian Hoh Edward C. Jauch Chelsea S. Kidwell Thabele M. Leslie-Mazwi Bruce Ovbiagele Phillip A. Scott Kevin N. Sheth Andrew M. Southerland Deborah V. Summers David L. Tirschwell 徐加平 刘慧慧 张霞 石际俊 黄志超 尤寿江 郭志良 肖国栋 杜万良 曹勇军 | 2018 | 国际脑血管病杂志2018，26，2： | 19 |
| 2 | Establishment of Withania somnifera Hairy Root Cultures for the Production of Withanolide A显示文摘Withania sominifera (Indian ginseng) was transformed by Agrobacterium rhizogenes. Explants from seedling roots, stems, hypocotyls, cotyledonary nodal segments, cotyledons and young leaves were inoculated with A. rhizogenes strain R1601. Hairy (transformed) roots were induced from cotyledons and leaf explants. The transgenic status of hairy roots was confirmed by polymerase chain reaction using nptII and rolB specific primers and, subsequently, by Southern analysis for the presence of nptII and rolB genes in the genomes of transformed roots. Four clones of hairy roots were established; these differed in their morphology. The doubling time of faster growing cultures was 8-14 d with a fivefold increase in biomass after 28 d compared with cultured, non-transformed seedling roots. MS-based liquid medium was superior for the growth of transformed roots compared with other culture media evaluated (SH, LS and N6), with MS-based medium supplemented with 40 g/L sucrose being optimal for biomass production. Cultured hairy roots synthesized withanolide A, a steroidal lactone of medicinal and therapeutic value. The concentration of withanolide A in transformed roots (157.4 μg/g dry weight) was 2.7-fold more than in non-transformed cultured roots (57.9 μg/g dry weight). | Hosakatte N. Murthy Camelia Dijkstra Paul Anthony Daniel A. White Mike R. Davey J. Brian Power Eun J. Hahn Kee Y. Paek | 2008 | Journal of Integrative Plant Biology2008，50，8： | 3 |
| 3 | Genetic transformation of wheat: progress during the 1990s into the Millennium显示文摘 | Heidi M. Ingram Nancy L. Livesey J. Brian Power Michael R. Davey | 2001 | Acta Physiologiae Plantarum2001，，2： | 1 |
| 4 | Novel approaches for regulating gas supply to plant systems in vitro: Application and benefits of artificial gas carriers显示文摘 | Kenneth C. Lowe Paul Anthony J. Brian Power Michael R. Davey | 2003 | In Vitro Cellular & Developmental Biology - Plant2003，，6： | 1 |
| 5 | Plant regeneration by somatic embryogenesis and organogenesis in commercial pineapple (Ananas comosus L.)显示文摘 | Suneerat Sripaoraya Robert Marchant J. Brian Power Michael R. Davey | 2003 | In Vitro Cellular & Developmental Biology - Plant2003，，5： | 1 |
| 6 | Expression of a chitinase transgene in rose (Rosa hybrida L.) reduces development of blackspot disease (Diplocarpon rosae Wolf)显示文摘 | Robert Marchant Michael R. Davey John A. Lucas Chris J. Lamb Richard A. Dixon J. Brian Power | 1998 | Molecular Breeding1998，，3： | 1 |
| 7 | Plant protoplasts: status and biotechnological perspectives显示文摘 | Michael R. Davey Paul Anthony J. Brian Power Kenneth C. Lowe | 2004 | Biotechnology Advances2004，，2： | 1 |
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