| 3 | Environmental drivers and genomic architecture of trait differentiation in fire-adapted Banksia attenuata ecotypes显示文摘Trait divergence between populations is considered an adaptive response to different environments,but to what extent this response is accompanied by genetic differentiation is less clear since it may be phenotypic plasticity. In this study, we analyzed phenotypic variation between two Banksia attenuata growth forms, lignotuberous(shrub) and epicormic resprouting(tree), in fire-prone environments to identify the environmental factors that have driven this phenotypic divergence. We linked genotype with phenotype and traced candidate genes using differential gene expression analysis. Fire intervals determined the phenotypic divergence between growth forms in B. attenuata. A genome-wide association study identified 69 single nucleotide polymorphisms, putatively associated with growth form, whereas no growth form-or phenotype-specific genotypes were eidentified. Genomic differentiation between the two growth forms was low(F_(st)=0.024). Differential gene expression analysis identified 37 genes/transcripts that were differentially expressed in the two growth forms. A small heat-shock protein gene, associated with lignotuber presence, was differentially expressed in the two forms.We conclude that different fire regimes induce phenotypic polymorphism in B. attenuata, whereas phenotypic trait divergence involves the differential expression of a small fraction of genes that interact strongly with the disturbance regime. Thus, phenotypic plasticity among resprouters is the general strategy for surviving varying fire regimes. | Tianhua He Byron B.Lamont Neal J.Enright Haylee M.D'Agui William Stock | 2019 | Journal of Integrative Plant Biology2019，61，4： | 0 |
| 4 | Characterization of Leaf Transcriptome in Banksia hookeriana显示文摘Banksia is a significant element in vegetation of southwestern Australia, a biodiversity hotspot with global significance. In particular, Banksia hookeriana represents a species with significant economic and ecological importance in the region. For better conservation and management,we reported an overview of transcriptome of B. hookeriana using RNA-seq and de novo assembly.We have generated a total of 202.7 million reads(18.91 billion of nucleotides) from four leaf samples in four plants of B. hookeriana, and assembled 59,063 unigenes(average size = 1098 bp)through de novo transcriptome assembly. Among them, 39,686 unigenes were annotated against the Swiss-Prot, Clusters of Orthologous Groups(COG), and NCBI non-redundant(NR) protein databases. We showed that there was approximately one single nucleotide polymorphism(SNP)per 5.6–7.1 kb in the transcriptome, and the ratio of transitional to transversional polymorphisms was approximately 1.82. We compared unigenes of B. hookeriana to those of Arabidopsis thaliana and Nelumbo nucifera through sequence homology, Gene Ontology(GO) annotation, and KEGG pathway analyses. The comparative analysis revealed that unigenes of B. hookeriana were closely related to those of N. nucifera. B. hookeriana, N. nucifera, and A. thaliana shared similar GO annotations but different distributions in KEGG pathways, indicating that B. hookeriana has adapted to dry-Mediterranean type shrublands via regulating expression of specific genes. In total 1927 potential simple sequence repeat(SSR) markers were discovered, which could be used in the genotype and genetic diversity studies of the Banksia genus. Our results provide valuable sequence resource for further study in Banksia. | Sim Lin Lim Haylee M.D'Agui Neal J.Enright Tianhua He | 2017 | Genomics, Proteomics & Bioinformatics2017，15，1： | 0 |
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