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您的检索式：作者名="J.Joe Hull"
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| 1 | Molecular cloning and comparative analysis of transcripts encoding chemosensory proteins from two plant bugs, Lygus lineolaris and Lygus hesperus显示文摘Chemosensory proteins(CSPs)are soluble carrier proteins typically characterized by a six‐helix bundle structure joined by two disulfide bridges and a conserved Cys spacing pattern(C1‐X6‐8‐C2‐X16‐21‐C3‐X2‐C4).CSPs are functionally diverse with reported roles in chemosensation,immunity,development,and resistance.To expand our molecular understanding of CSP function in plant bugs,we used recently developed transcriptomic resources for Lygus lineolaris and Lygus hesperus to identify 17 and 14 CSP‐like sequences,respectively.The Lygus CSPs are orthologous and share significant sequence identity with previously annotated CSPs.Three of the CSPs are predicted to deviate from the typical CSP structure with either five or seven helical segments rather than six.The seven helix CSP is further differentiated by an atypical C3‐X3‐C4 Cys spacing motif.Reverse transcriptase PCR‐based profiling of CSP transcript abundance in adult L.lineolaris tissues revealed broad expression for most of the CSPs with antenna specific expression limited to a subset of the CSPs.Comparative sequence analyses and homology modeling suggest that variations in the amino acids that comprise the Lygus CSP binding pockets affect the size and nature of the ligands accommodated. | J.Joe Hull Omaththage P.Perera Mei-Xian Wang | 2020 | Insect Science2020，27，3： | 1 |
| 2 | Efficient nanoparticle-based CRISPR-Cas13d induced mRNA disruption of an eye pigmentation gene in the white-backed planthopper,Sogatella furcifera显示文摘The discovery of the clustered regularly interspaced short palindromic repeat(CRISPR)system has driven gene manipulation technology to a new era with applications reported in organisms that span the tree of life.The utility of CRISPR-mediated editing was further expanded to mRNA following identification of the RNA-targeting Cas13 family of smaller endonuclease proteins.Application of this family to insect research,however,has been more limited.In this study,the smallest Cas13 family member,Cas13d,and guide RNAs(gRNAs)were complexed with a versatile nanomaterial(star polycation,SPc)to generate a proof-of-concept RNA-editing platform capable of disrupting mRNA expression of the eye pigmentation gene tryptophan 2,3-dioxygenase(SfTO)in white-backed planthoppers(WBPHs).The resulting red-eye phenotype was present in 19.76%(with SPc)and 22.99%(without SPc)of the treatment groups and was comparable to the red-eye phenotype generated following conventional RNA interference knockdown(22.22%).Furthermore,the Cas13/gRNA phenotype manifested more quickly than RNA interference.Consistent with the expected Cas13d mechanism,SfTO transcript levels were significantly reduced.Taken together,the results indicate that the SPc-CRISPR-Cas13d/gRNA complex negatively impacted expression of the target gene.These findings confirm the utility of this novel mRNA disruption system in insects and lay the foundation for further development of these tools in the implementation of green agricultural pest management tactics. | Yun-Feng Ma Meng-Qi Zhang Lang-Lang Gong Xuan-Zheng Liu Gui-Jun Long Huan Guo J.Joe Hull Youssef Dewer Ming He Peng He | 2023 | Insect Science2023，30，6： | 0 |
| 3 | Assessment of suitable reference genes for qRT-PCR analysis in Adelphocoris suturalis显示文摘Quantitative reverse transcription polymerase chain reaction(qRT-PCR) is the most commonly-used tool for measurement of gene expression, but its accuracy and reliability depend on appropriate data normalization with the use of one or more stable reference genes. Adelphocoris suturalis is one of the most destructive pests of cotton, but until recently knowledge of its underlying molecular physiology had been hindered by a lack of molecular resources. To facilitate research on this pest, we evaluated 12 common housekeeping genes studied in insects(GAPDH, ACT, βACT, TBP, SDH, βTUB, EF1γ, EF1α, EF1δ, RPL32, RPS15, and RPL27) for their expression stability in A. suturalis when subjected to various experimental treatments, including three biotic(developmental stage and sex, tissue type, and metathoracic scent gland for varying developmental stages and sexes) and one abiotic(RNA interference injection) conditions. Four dedicated algorithms(ΔCt method, geNorm, BestKeeper and NormFinder) were used to analyze gene expression stability. In addition, RefFinder provided an overall ranking of the stability/suitability of these candidates. This study is the first to provide a comprehensive list of suitable reference genes for gene expression analyses in A. suturalis, which can serve to facilitate transcript expression study of related biological processes in this and related species. | LUO Jing MA Chao LI Zhe ZHU Bang-qin ZHANG Jiang LEI Chao-liang JIN Shuang-xia J.Joe Hull CHEN Li-zhen | 2018 | Journal of Integrative Agriculture2018，17，12： | 0 |
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