|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 查看详情显示文摘 | S.Salzbrum J.Simon G.K.S.Prakash | | 0，，： | 1 |
| 2 | Variation in Defence Strategies in Two Species of the Genus Beilschmiedia under Differing Soil Nutrient and Rainfall Conditions显示文摘 | J.Simon R. E.Miller I. E.Woodrow | 2008 | Plant Biology2008，，1： | 1 |
| 3 | Mechanism of butane skeletal isomerization on sulfated zirconia显示文摘 | Xuebing Li Katsutoshi Nagaoka Laurent J.Simon | | 0，，02： | 1 |
| 4 | Effects of BDNF,T3,and corticosterone on expression of the hypothalamic obesity gene network in vivo and in vitro显示文摘 | Byerly M.S J.Simon E.Lebihan-Duval M.J.Duclos L.A.Cogburn and T.E.Porter | | 0，，： | 1 |
| 5 | Functional genomics in chickens:Development of integrated-systems microarrays for transcriptional profiling and discovery of regulatory pathways显示文摘 | Cogburn L.A X.Wang W.CarTe L.Rejto S.E.Aggrey M.J.Duclos J.Simon and T.E.Porter | | 0，，： | 1 |
| 6 | Evaluation of mulitprobe radiofrequency technology in a porcine model显示文摘 | WILLIAM W.HOPE JASON M.ARRU JASON Q.MCKEE DENNISVROCHIDES BASSAMASWAD CAROLINE J.SIMON DAMIAN E.DUPUY DAVID A.IANNITTI | 2009 | HPB2009，，5： | 1 |
| 7 | Pharmacological applications of a novel neoepitope antibody to a modified amyloid precursor protein-derived beta-secretase product显示文摘We have previously described a novel artificial NFEVβ-secretase(BACE1)cleavage site,which when introduced into the amyloid-βprecursor protein(APP),significantly enhances APP cleavage by BACE1 in in vitro and cellular assays.In this study,we describe the identification and characterization of a single chain fragment of variable region(scFv),specific to the EV neo-epitope derived from BACE1 cleavage of the NFEV-containing peptide,and its conversion to IgG1.Both the scFv displayed on phage and EV-IgG1 show exquisite specificity for binding to the EV neoepitope without cross-reactivity to other NFEV containing peptides or WT-APP KMDA cleavage products.EV-IgG1 can detect as little as 0.3 nmol/L of the EV peptide.EV-IgG1 antibody was purified,conjugated with alkaline phosphatase and utilized in various biological assays.In the BACE1 enzymatic assay using NFEV substrate,a BACE1 inhibitor MRK-3 inhibited cleavage with an IC50 of 2.4 nmol/L with excellent reproducibility.In an APP_NFEV stable SH-SY5Y cellular assay,the EC_(50) for inhibition of EV-Aβ peptide secretion with MRK-3 was 236 nmol/L,consistent with values derived using an EV polyclonal antibody.In an APP_NFEV knock-in mouse model,both Aβ_EV40 and Aβ_EV42 peptides in brain homogenate showed excellent gene dosage dependence.In conclusion,the EV neoepitope specific monoclonal antibody is a novel reagent for BACE1 inhibitor discovery for both in vitro,cellular screening assays and in vivo biochemical studies.The methods described herein are generally applicable to novel synthetic substrates and enzyme targets to enable robust screening platforms for enzyme inhibitors. | Guoxin Wu Sethu Sankaranarayanan Donna L.Montgomery Adam J.Simon Zhiqiang An Mary J.Savage | 2011 | Protein & Cell2011，2，7： | 0 |
| 8 | A good gut feeling on Sarml-mediated axon degeneration显示文摘Axons,the cable-like projections of neurons,experience a wide range of stressors throughout their lifetimes.The result of any one of these challenges can be catastrophic,promoting degeneration of the axon.Axon degeneration is an active signaling process observed in traumatic brain injury,chemotherapy-induced peripheral neuropathies,and neu-rodegenerative disease(Simon and Watkins,2018;Coleman and Hoeke,2020). | Victoria E.von Saucken David J.Simon | 2021 | Protein & Cell2021，12，8： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员