| 1 | Exosomes derived from circBCRC-3-knockdown mesenchymal stemcells promoted macrophage polarization显示文摘Macrophages play an essential role in the myocardial ischemia-reperfusion injury(MIRI),and the macrophage shifting from M1 to M2 phenotypes might be a potential strategy for the treatment of MIRI.It has been reported that miR-182 plays an important role in MSC-Exo-associated macrophage polarization.As circBCRC-3 is a newly discovered circle RNA that worked as a sponge of miR-182,this research aimed to find if circBCRC-3 plays a role in MSC-Exo-associated macrophage polarization.Firstly,circBCRC-3 was identified by divergent primers in mesenchymal stem cells(MSCs).Secondly,the exosome of MSCs was isolated and identified by transmission electron microscopy(TEM),nanoparticle-tracking analysis,and western blotting analysis.The expression level of circBCRC-3 in MSCexos was detected by RT-PCR.Finally,the polarization of the RAW264.7 cell phenotype was analyzed by flow cytometry.Moreover,we first identified circBCRC-3 in MSCs.The results further confirmed that MSCexo could effectively shift the macrophage polarization state from M1 towards the M2 phenotype,which indicated its role in MIRI cure. | QI SONG JUN ZHANG QIANG ZHANG JING LIU KE LV JIALU YAO YAFENG ZHOU | 2020 | BIOCELL2020，44，4： | 0 |
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