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| 1 | Absolute dominance of hydrogenotrophic methanogens in full-scale anaerobic sewage sludge digesters显示文摘Anaerobic digestion(AD)is gaining increasing attention due to the ability to covert organic pollutants into energy-rich biogas and,accordingly,growing interest is paid to the microbial ecology of AD systems.Despite extensive eforts,AD microbial ecology is still limitedly understood,especially due to the lack of quantitative information on the structures and dynamics of AD microbial communities.Such knowledge gap is particularly pronounced in sewage sludge AD processes although treating sewage sludge is among the major practical applications of AD.Therefore,we examined the microbial communities in three full-scale sewage sludge digesters using qualitative and quantitative molecular techniques in combination:denaturing gradient gel electrophoresis(DGGE)and real-time polymerase chain reaction(PCR).Eight out of eleven bacterial sequences retrieved from the DGGE analysis were not afliated to any known species while all eleven archaeal sequences were assigned to known methanogen species.Quantitative real-time PCR analysis revealed that,based on the 16S rRNA gene abundance,the hydrogenotrophic order Methanomicrobiales is the most dominant methanogen group(>94%of the total methanogen population)in all digesters.This corresponds well to the prevailing occurrence of the DGGE bands related to Methanolinea and Methanospirillum,both belonging to the order Methanomicrobiales,in all sludge samples.It is therefore suggested that hydrogenotrophic methanogens,especially Methanomicrobiales strains,are likely the major players responsible for biogas production in the digesters studied.Our observation is contrary to the conventional understanding that aceticlastic methanogens generally dominate methanogen communities in stable AD environments,suggesting the need for further studies on the dominance relationship in various AD systems. | Jaai Kim Woong Kim Changsoo Lee | 2013 | Journal of Environmental Sciences2013，25，11： | 2 |
| 2 | Isolation and identification of thiocyanate utilizing chemolithotrophs from gold mine soils显示文摘 | JAAI KIM JINSOO CHANG | 2003 | Biodegradation2003，，14： | 1 |
| 3 | Effect of rocuronium compared with succinylcholine on intraocular pressure during rapid sequence induction of anaesthesia 显示文摘 | Chiu CL Jaais F Wang CY | 1999 | Br J Anaesth1999，82，： | 1 |
| 4 | Trabeculectomy - a review and 2 year follow up 显示文摘 | Jaais F | 2004 | Med J Malaysia2004，59，3： | 1 |
| 5 | Effect of roeuronium compared with succinylcholine on intraocular pressure during rapid sequence induction of anaesthesia显示文摘 | Chiu CL Jaais F Wang CY | 1999 | Br J Anaesth1999，82，5： | 1 |
| 6 | Trabeculectomy-a review and 2 year follow up显示文摘 | Jaais F | 2004 | Med J Malaysia2004，59，3： | 1 |
| 7 | Trabeculectomy-a review and 2 year follow up显示文摘 | Jaais F | 2004 | Med J Malaysia2004，59，3： | 1 |
| 8 | A prognostics and health management roadmap for information and electronics-rich systems显示文摘 | PECHT M JAAI R | 2010 | Microelectronics Reliability2010，50，3： | 1 |
| 9 | A prognostics and health man- agement roadmap for information and electronics-rich systems 显示文摘 | Michael Pecht Rubyca Jaai | 2010 | Microelectronics Reliability2010，50，： | 1 |
| 10 | Thermo-alkaline pretreatment of waste activated sludge at low-temperatures: Effects on sludge disintegration, methane production, and methanogen community structure显示文摘 | Jaai Kim Youngseob Yu Changsoo Lee | 2013 | Bioresource Technology2013，，： | 1 |
| 11 | A prognostics and health management roadmap for information and electronics-rich systems显示文摘 | Pecht M Jaai R | 2010 | Microelectronics Reliability2010，50，3： | 1 |
| 12 | Do Immigrants Save Less than Natives? Immigrant and Native Saving Behaviour in Australia显示文摘 | Asadul Islam Jaai Parasnis Dietrich Fausten | 2013 | Econ Rec2013，，284： | 1 |
| 13 | Isolation and identification of thiocyanate utilizing chemolithotrophs from gold mine soils显示文摘 | JAAI KIM JINSOO CHANG | 2003 | Biodegradation2003，，14： | 1 |
| 14 | 有趣小故事慈善大事件显示文摘慈善无小事,生活中有很多有趣又可爱的慈善事件值得我们盘点一番!一起来看看吧! | Jaay Adriannae | 2015 | 小读者2015，0，2： | 0 |
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